scholarly journals The Role of Follicular Fluid Anti-Mullerian Hormone in Success Rate of Intracytoplasmic Sperm Injection

2020 ◽  
Vol 8 (B) ◽  
pp. 962-965
Author(s):  
Hiathm Ahmed Baha El-Din ◽  
Essam Ahmed El Gindy ◽  
Ahmed Kotb Ahmed ◽  
Osama Ahmed Ibrahim ◽  
Ayman Nady Abdelmegeed
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Ovarian Function ◽  
Implantation Rate ◽  
Inverse Correlation ◽  
Clinical Pregnancy ◽  
University Hospital ◽  
Blastocyst Development ◽  
Vitro Fertilization

BACKGROUND: The role of anti-Mullerian hormone (AMH) in the ovary is to participate in the regulation of ovarian function, especially in follicle development and selection. It inhibits the initiation of human primordial follicle growth and prevents multiple selection of a dominant follicle by reducing the sensitivity of follicles to follicle stimulating hormone. MATERIALS AND METHODS: In this prospective clinical trial, outcomes were followed in 60 women undergoing cycles of in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) within El-Minia University Hospital. AMH concentration was estimated in pooled follicle fluid (FF) on day of oocyte pickup. Cycles were sorted into low and high groups according to median (50th centile) values of measurement. The fertilization rate (FR), implantation rate, blastocyst development, embryo quality, chemical pregnancy, clinical pregnancy, and ongoing pregnancy after ICSI were counted as the main outcomes. RESULTS: Low FF AMH group shows significantly higher percentage of top-quality oocytes (67.1 ± 24.3 vs. 49.6 ± 30.3 %, p = 0.014), FR (83.9 ± 20.9 vs. 72.4 ± 21.4%, p = 0.021), clinical pregnancy (57.57 vs. 16.67%, p > 0.0001), and embryo implantation rates (57.7 vs. 16.7%, p = 0.001) compared to high FF AMH group. FF AMH shares an inverse correlation with FF E2 (Pearson r = −0.409, p < 0.001) and clinical pregnancy (Pearson r = −0.618, p < 0.001). Threshold value of FF AMH for pregnancy is >1.75 ng/mg protein. CONCLUSION: FF AMH is a plausible specific indicator of functional viability and quality of oocyte in IVF cycles.  

Role of Hysteroscopy in Repeated IVF/ICSI – ETs Failure and its Impact Effect on Subsequent Attempts

2021 ◽  
Vol 19 (5) ◽  
pp. 86-94
Author(s):  
Shaima Gaber Mohmeed Gadalla Elgebally ◽  
Mahmoud Salah El Din Hamouda ◽  
Ahmed Tawfeek Soliman ◽  
Dina Gamal El Deen El-Kholi
Keyword(s):  
Prospective Observational Study ◽  
Uterine Cavity ◽  
Clinical Pregnancy ◽  
University Hospital ◽  
Cervical Canal ◽  
Vitro Fertilization ◽  
Reproductive Unit ◽  
Infertile Patients

Background: Recurrent implantation breakdown is characterized as a complication to accomplish a clinical pregnancy subsequent to transfer of 4 or further high-quality embryos in a least amount of 2 in vitro fertilization (IVF) cycles in a woman less than the age of 40. This investigation intended to the responsibility of hysteroscopy in repeated IVF/ICSI failure and its impact on subsequent trials. Methods: Prospective observational study was conducted at assisted reproductive unit in obstetric and gynecology department in Tanta University Hospital from Jan 2018 to Jan 2020. Included 50 infertile patients with more then two IVF/ICSI failure in spite of transmit of as a minimum two better quality embryos. All patients were subjected to 4D US examination with same device (Siemens acuson X300). Results: We found that from the 30 patients who had normal findings in hysteroscopy, 25 had normal findings also in 4DUS, 2 had polyps, 2 had myoma and 1 patient had septum. From the 3 patients who had polyps in hysterectomy, 1 had normal findings in 4DUS, and 2 patients had polyps. Regarding the two patients who had myoma by hysteroscopy, 1 had normal findings in 3DUS and the other had Myoma. While for the 3 considered patients who had septum in hysteroscopy, all had normal findings in 4DUS. Conclusion: According to this study, hysteroscopy is regarded the gold standard not only for envisaging the cervical canal and uterine cavity, however also for healing several types of benign pathologies localized to that area, it is a secure and uncomplicated process.

scholarly journals Should rescue ICSI be re-evaluated considering the deferred transfer of cryopreserved embryos in in-vitro fertilization cycles? A systematic review and meta-analysis

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Alessio Paffoni ◽  
Marco Reschini ◽  
Valerio Pisaturo ◽  
Cristina Guarneri ◽  
Simone Palini ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Implantation Rate ◽  
Clinical Pregnancy ◽  
Frozen Embryo Transfer ◽  
Fertilization Failure ◽  
Vitro Fertilization ◽  
Rescue Icsi

Abstract Background Total fertilization failure represents a particularly frustrating condition for couples undergoing in vitro fertilization. With the aim of reducing the occurrence of total fertilization failure, intracytoplasmic sperm injection (ICSI) has become the first choice over conventional in vitro fertilization (IVF) procedures although evidence of improved results is still debated and its use in couples without male factor infertility is not recommended. Among the strategies potentially useful to promote the use of conventional IVF, we herein call attention to the late rescue ICSI, which consists in performing ICSI after 18–24 h from conventional insemination on oocytes that show no signs of fertilization. This treatment has however been reported to be associated with a low success rate until recent observations that embryos derived from late rescue ICSI may be transferred after cryopreservation in a frozen-thawed cycle with improved results. The aim of the present study was to assess whether frozen embryos deriving from rescue ICSI performed about 24 h after conventional IVF may represent a valuable option for couples experiencing fertilization failure. Methods A systematic review on the efficacy of late rescue ICSI was performed consulting PUBMED and EMBASE. Results Including twenty-two original studies, we showed that clinical pregnancy rate per embryo transfer and implantation rate obtainable with fresh embryo transfers after rescue ICSI are not satisfactory being equal to 10 and 5%, respectively. The transfer of cryopreserved rescue ICSI embryos seems to offer a substantial improvement of success rates, with pregnancy rate per embryo transfer and implantation rate equal to 36 and 18%, respectively. Coupling rescue ICSI with frozen embryo transfer may ameliorate the clinical pregnancy rate for embryo transfer with an Odds Ratio = 4.7 (95% CI:2.6–8.6). Conclusion Results of the present review support the idea that r-ICSI coupled with frozen embryo transfer may overcome most of the technical and biological issues associated with fresh transfer after late r-ICSI, thus possibly representing an efficient procedure for couples experiencing fertilization failure following conventional IVF cycles. Trial registration Prospero registration ID: CRD42021239026.

scholarly journals Results and experiences after 1000 IVF cycles at the Clinic for gynecology and obstetrics in Novi Sad

2011 ◽  
Vol 64 (11-12) ◽  
pp. 565-569
Author(s):  
Vesna Kopitovic ◽  
Stevan Milatovic ◽  
Aleksandra Trninic-Pjevic ◽  
Artur Bjelica ◽  
Irena Bujas ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Intracytoplasmic Sperm Injection ◽  
Clinical Pregnancy ◽  
Reproductive Age ◽  
Male Factor ◽  
Fertilization Program ◽  
Vitro Fertilization ◽  
Gynecology And Obstetrics ◽  
Novi Sad

Introduction. Infertility affects 15-17% of reproductive age couples in our country, and 10-15% of couples worldwide. The aim of this paper was to present results and experience gained after the first 1000 cycles of the national In Vitro Fertilization (IVF) program, to offer professional standard of work in our country and to compare it with results obtained in Europe and worldwide. Material and methods. The study prospectively included 1000 women who had undergone national In Vitro Fertilization program from October 2006 until November 2009 at the Department of Gynecology and Obstetrics, Clinical Center of Vojvodina, Novi Sad. Results. The analysis included 1000 in vitro fertilization cycles. Male factor infertility was the leading cause (56.9%) followed by tubal factor (45.9%). The classic method of in vitro fertilization constituted 72.3% of all cycles, while intracytoplasmic sperm injection (ICSI) method was used in 27.7% of all cycles. The average number of embryos transferred was 2.67. The cycle cancellation rate was 14.10% and the aspiration rate was 94.40%. The clinical pregnancy and live birth rate were 33.41% and 26.78% per embryo transfer, respectively. Discussion and conclusion. The results in our study showed that in our setting there are far less intracytoplasmic sperm injection cycles compared with the European average of 66.5% of all fresh cycles, and that we transferred more embryos on average. Our success rates are comparable with those in other European countries where the clinical pregnancy rates per aspiration and per transfer for in vitro fertilization were 29 and 32.4%, respectively in the period of observation. For intracytoplasmic sperm injection, the corresponding rates were 29.9 and 33%.

scholarly journals The Role of Interleukin-18 in Serum and Follicular Fluid during In Vitro Fertilization and Intracytoplasmic Sperm Injection

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Veronika Günther ◽  
Ibrahim Alkatout ◽  
Corinna Fuhs ◽  
Ali Salmassi ◽  
Liselotte Mettler ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Intracytoplasmic Sperm Injection ◽  
Ovarian Stimulation ◽  
The Body ◽  
Interleukin 18 ◽  
Vitro Fertilization ◽  
Median Level

Cytokines are key modulators of the immune system and play an important role in the ovarian cycle. IL-18 levels in serum and follicular fluid were analyzed in women undergoing in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) treatment. The cohort study group consisted of 90 women, who were undergoing IVF or ICSI. The body mass index (BMI) was determined in all patients; IL-18 levels were measured in follicular fluid and serum. IL-18 levels in serum were significantly higher than those in follicular fluid. The median level in serum was 162.75 (80.21) pg/mL and that in follicular fluid, 138.24 (91.78) pg/mL. Women undergoing IVF treatment had lower IL-18 levels in serum (median, 151.19 (90.73) pg/mL) than those treated with ICSI (median, 163.57 (89.97) pg/mL). The correlation between IL-18 levels in serum and BMI was statistically significant, as well as the correlation between IL-18 levels in follicular fluid and ovarian stimulation response (p=0.003). IL-18 was correlated with the response to ovarian stimulation and was the reason for successful pregnancy after IVF or ICSI treatment. Among other cytokines, IL-18 appears to be a promising prognostic marker of success in reproductive treatment and should be evaluated as such in further prospective studies.

The role of diagnostic hysteroscopy before the first in vitro fertilization/intracytoplasmic sperm injection cycle

2012 ◽  
Vol 286 (5) ◽  
pp. 1323-1328 ◽  
Author(s):  
Hsing-Tse Yu ◽  
Chin-Jung Wang ◽  
Chyi-Long Lee ◽  
Hong-Yuan Huang ◽  
Chun-Kai Chen ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Intracytoplasmic Sperm Injection ◽  
Vitro Fertilization ◽  
Diagnostic Hysteroscopy ◽  
Injection Cycle

scholarly journals 146COMPARISON OF EMBRYO CULTURE MEDIA FOR BLASTOCYST DEVELOPMENT AFTER INTRACYTOPLASMIC SPERM INJECTION OF IN VITRO-MATURED EQUINE OOCYTES

2004 ◽  
Vol 16 (2) ◽  
pp. 195
Author(s):  
Y.H. Choi ◽  
D.D. Varner ◽  
K. Hinrichs
Keyword(s):  
In Vitro Culture ◽  
Embryo Culture ◽  
Intracytoplasmic Sperm Injection ◽  
Culture Media ◽  
Polar Body ◽  
Developmental Competence ◽  
Blastocyst Development ◽  
Vitro Fertilization ◽  
Significant Difference

Research on in vitro culture of equine embryos has been scant, due to failure of equine in vitro fertilization to be repeatably successful. We have recently obtained high fertilization rates of equine oocytes via intracytoplasmic sperm injection (ICSI) using a piezo drill (Choi et al., 2002 Reproduction 123, 455–465). Culture of presumptive zygotes in G1.2/2.2 medium resulted in 63% cleavage and an average of 15 cells at 4d, but only 2 to 9% blastocyst development at 7 days (Choi et al., 2003 Theriogenology 59, 1219–1229). In the present study, we evaluated the effect of two different culture media, G1.3/G2.3 v. DMEM/F-12, with or without FBS, on blastocyst development after ICSI. Oocytes were collected from slaughterhouse-derived ovaries by follicular scraping and were matured in vitro for 24h in M199 with 10% FBS and 5μUmL−1 FSH. After culture, oocytes having a polar body (198/305; 65%) were fertilized by ICSI with frozen-thawed equine sperm using a piezo drill. Presumptive zygotes were cultured in 1 of 4 media: G1.3/G2.3 (which includes 0.8% BSA) with or without 10% FBS, or in DMEM/F-12 with 0.5% BSA, with or without 10% FBS. Culture was performed in microdroplets at 5μL/zygote under oil at 38.2°C in an atmosphere of 5% CO2, 5% O2 and 90% N2 for 7.5 days. In G1.3/2.3 treatments, G1.3 media were completely refreshed at 48h, zygotes were transferred to G2.3 (with or without FBS as per the first stage) at 96h, and were completely refreshed with the same media at 144h. In DMEM/F-12 treatments, media were completely refreshed every other day. Three to 5 replicates were performed in each treatment, and data were analyzed by chi-square test. There were no significant differences in cleavage rates (59–64%) among treatments. The rate of development to blastocyst, per oocyte injected, in G1.3/G2.3/BSA (1/49, 2%) was significantly lower (P&lt;0.05) than that for the other three treatments: G1.3/2.3/BSA/FBS (9/49, 18%), DMEM/F-12/BSA (9/50, 18%), or DMEM/F-12/BSA/FBS (10/50, 20%). There was no significant difference in blastocyst development among the latter three treatments. These findings indicate that G1.3/2.3 media with BSA only do not adequately support growth of equine embryos. Development of up to 20% of injected oocytes to the blastocyst stage in G media supplemented with FBS, in DMEM/F-12/BSA or in DMEM/F-12/BSA/FBS represents the highest in vitro equine blastocyst rate in medium alone (i.e. without co-culture) yet reported. The success of DMEM/F-12 as an embryo culture medium may provide a relatively simple basis for equine in vitro culture programs. To determine whether this medium was able to support further developmental competence, we cultured equine embryos resulting from nuclear transfer of in vitro-matured oocytes in DMEM/F-12+10% FBS (without BSA). We transferred 4 resulting blastocysts to recipient mares by transcervical transfer; one pregnancy is ongoing at 230d gestation at the time of this writing. This work was supported by the Link Equine Research Endowment Fund, Texas A&amp;M University.

Sign in / Sign up

Export Citation Format

Share Document