scholarly journals Anti-obesity effects of yellow catfish protein hydrolysate on mice fed a 45% kcal high-fat diet

2017 ◽  
Vol 40 (3) ◽  
pp. 784-800 ◽  
Author(s):  
Mi-Ryung Kim ◽  
Joo-Wan Kim ◽  
Jeong Been Park ◽  
Yong-Ki Hong ◽  
Sae Kwang Ku ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Protein Hydrolysate ◽  
High Fat ◽  
Yellow Catfish

Role of potato protein hydrolysate and exercise in preventing high‐fat diet‐induced hepatocyte apoptosis in senescence‐accelerated mouse

2020 ◽  
Vol 44 (12) ◽  
Author(s):  
Ho‐Lin Chuang ◽  
Rathinasamy Baskaran ◽  
Cecilia Hsuan Day ◽  
Yueh‐Min Lin ◽  
Chih‐Chu Ho ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Protein Hydrolysate ◽  
Hepatocyte Apoptosis ◽  
High Fat ◽  
Potato Protein ◽  
Senescence Accelerated Mouse

scholarly journals Herring Milt and Herring Milt Protein Hydrolysate Are Equally Effective in Improving Insulin Sensitivity and Pancreatic Beta-Cell Function in Diet-Induced Obese- and Insulin-Resistant Mice

Marine Drugs ◽  
2020 ◽  
Vol 18 (12) ◽  
pp. 635
Author(s):  
Yanwen Wang ◽  
Sandhya Nair ◽  
Jacques Gagnon
Keyword(s):  
Insulin Resistance ◽  
High Fat Diet ◽  
Cell Function ◽  
Protein Hydrolysate ◽  
High Fat ◽  
Β Cell ◽  
Insulin Resistant ◽  
Β Cell Function ◽  
Casein Protein ◽  
Dietary Casein

Although genetic predisposition influences the onset and progression of insulin resistance and diabetes, dietary nutrients are critical. In general, protein is beneficial relative to carbohydrate and fat but dependent on protein source. Our recent study demonstrated that 70% replacement of dietary casein protein with the equivalent quantity of protein derived from herring milt protein hydrolysate (HMPH; herring milt with proteins being enzymatically hydrolyzed) significantly improved insulin resistance and glucose homeostasis in high-fat diet-induced obese mice. As production of protein hydrolysate increases the cost of the product, it is important to determine whether a simply dried and ground herring milt product possesses similar benefits. Therefore, the current study was conducted to investigate the effect of herring milt dry powder (HMDP) on glucose control and the associated metabolic phenotypes and further to compare its efficacy with HMPH. Male C57BL/6J mice on a high-fat diet for 7 weeks were randomized based on body weight and blood glucose into three groups. One group continued on the high-fat diet and was used as the insulin-resistant/diabetic control and the other two groups were given the high-fat diet modified to have 70% of casein protein being replaced with the same amount of protein from HMDP or HMPH. A group of mice on a low-fat diet all the time was used as the normal control. The results demonstrated that mice on the high-fat diet increased weight gain and showed higher blood concentrations of glucose, insulin, and leptin, as well as impaired glucose tolerance and pancreatic β-cell function relative to those on the normal control diet. In comparison with the high-fat diet, the replacement of 70% dietary casein protein with the same amount of HMDP or HMPH protein decreased weight gain and significantly improved the aforementioned biomarkers, insulin sensitivity or resistance, and β-cell function. The HMDP and HMPH showed similar effects on every parameter except blood lipids where HMDP decreased total cholesterol and non-HDL-cholesterol levels while the effect of HMPH was not significant. The results demonstrate that substituting 70% of dietary casein protein with the equivalent amount of HMDP or HMPH protein protects against obesity and diabetes, and HMDP is also beneficial to cholesterol homeostasis.

scholarly journals Herring Milt and Herring Milt Protein Hydrolysate Reduce Weight Gain and Improve Insulin Sensitivity and Pancreatic Beta-Cell Function in Diet-Induced Obese Mice

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1699-1699
Author(s):  
Yanwen Wang ◽  
Sandhya Nair ◽  
Jacques Gagnon
Keyword(s):  
Insulin Resistance ◽  
High Fat Diet ◽  
Cell Function ◽  
Protein Hydrolysate ◽  
Oral Glucose ◽  
High Fat ◽  
Low Fat ◽  
Insulin Resistant ◽  
Low Fat Diet ◽  
Dietary Casein

Abstract Objectives The present study was designed to examine the effect of herring milt dry powder (HMDP) on glucose homeostasis and related metabolic phenotypes and compare its efficacy with herring milt protein hydrolysate (HMPH) in diet-induced obese and insulin resistant mice. Methods Male C57BL/6 J mice were pretreated with a high-fat diet for 7 weeks were divided into 3 groups where one group continued on the high-fat diet and used as the obese and insulin resistant control (HFC) and the other two groups were fed a modified HFC diet where 70% of casein was replaced with an equal percentage of protein derived from HMDP or HMPH. A group of mice fed a low-fat diet all the time was used as the normal or low-fat control (LFC). Body weight was obtained weekly and food intake was recorded daily. Semi-fating (4–6 hr) blood glucose was measured every other week using a glucometer using the blood from tail vein. Oral glucose tolerance was measured twice during weeks 5 and 9, respectively, and insulin tolerance was determined during week 7 of the treatment. At the end of the experiment, serum was obtained following overnight fasting for the measurement of fasting insulin, leptin, free fatty acids and lipids as well as other glucose metabolism-related biomarkers. Results During the 9-week treatment period, mice on the high-fat diet maintained significantly higher body weight and semi-fasting blood glucose levels and exhibited impaired oral glucose tolerance and insulin resistance relative to mice on the low-fat diet. At the end of the study, the analysis of fasting blood samples revealed that mice on the high-fat diet had increases in serum insulin, leptin, free fatty acids and cholesterol levels. Mice fed the high-fat diet also showed an increase in insulin resistance index and a decrease in β-cell function index. Compared to mice on the high-fat diet, the 70% replacement of dietary casein with an equal percentage of protein derived from HMDP or HMPH reversed or markedly improved these parameters, and HMDP and HMPH showed similar effects. Conclusions The results demonstrate that replacing dietary casein with the same amount of protein derived from either HMDP or HMPH prevents and improves high-fat-diet-induced obesity and insulin resistance. Funding Sources Atlantic Canada Opportunity Agency through the Atlantic Innovation Fund grant (no. 193,594) and National Research Council of Canada – NHP program.

scholarly journals Chlorella Protein Hydrolysate Attenuates Glucose Metabolic Disorder and Fatty Liver in High-fat Diet-induced Obese Mice

2016 ◽  
Vol 65 (7) ◽  
pp. 613-620 ◽  
Author(s):  
Naoto Noguchi ◽  
Teruyoshi Yanagita ◽  
Shaikh Mizanoor Rahman ◽  
Yotaro Ando
Keyword(s):  
Fatty Liver ◽  
High Fat Diet ◽  
Metabolic Disorder ◽  
Protein Hydrolysate ◽  
Obese Mice ◽  
High Fat

scholarly journals Protein hydrolysate from potato confers hepatic-protection in hamsters against high fat diet induced apoptosis and fibrosis by suppressing Caspase-3 and MMP2/9 and by enhancing Akt-survival pathway

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Shibu Marthandam Asokan ◽  
Tsu-Han Hung ◽  
Zong-Yan Li ◽  
Wen-Dee Chiang ◽  
Wan-Teng Lin
Keyword(s):  
Liver Damage ◽  
High Fat Diet ◽  
Interstitial Fibrosis ◽  
Protein Hydrolysate ◽  
Serum Markers ◽  
Tunel Staining ◽  
High Fat ◽  
Molecular Events ◽  
Extracellular Signal ◽  
Induced Apoptosis

Abstract Background A potato protein hydrolysate, APPH is a potential anti-obesity diet ingredient. Since, obesity leads to deterioration of liver function and associated liver diseases, in this study the effect of APPH on high fat diet (HFD) associated liver damages was investigated. Methods Six week old male hamsters were randomly separated to six groups (n = 8) as control, HFD (HFD fed obese), L-APPH (HFD + 15 mg/kg/day of APPH), M-APPH (HFD + 30 mg/kg/day), H-APPH (HFD + 75 mg/kg/day of APPH) and PB (HFD + 500 mg/kg/day of probucol). HFD fed hamsters were administered with APPH 50 days through oral gavage. The animals were euthanized and the number of apoptotic nuclei in liver tissue was determined by TUNEL staining and the extent of interstitial fibrosis was determined by Masson’s trichrome staining. Modulation in the molecular events associated with apoptosis and fibrosis were elucidated from the western blotting analysis of the total protein extracts. Results Hamsters fed with high fat diet showed symptoms of liver damage as measured from serum markers like alanine aminotransferase and aspartate aminotransferase levels. However a 50 day long supplementation of APPH effectively ameliorated the effects of HFD. HFD also modulated the expression of survival and apoptosis proteins in the hamster liver. Further the HFD groups showed elevated levels of fibrosis markers in liver. The increase in fibrosis and apoptosis was correlated with the increase in the levels of phosphorylated extracellular signal-regulated kinases (pERK1/2) revealing a potential role of ERK in the HFD mediated liver damage. However APPH treatment reduced the effect of HFD on the apoptosis and fibrosis markers considerably and provided hepato-protection. Conclusion APPH can therefore be considered as an efficient therapeutic agent to ameliorate high fat diet related liver damages.

scholarly journals Effects of Fat and Fatty Acids on the Formation of Autolysosomes in the Livers from Yellow Catfish Pelteobagrus Fulvidraco

Genes ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 751 ◽  
Author(s):  
Wu ◽  
Wei ◽  
Yang ◽  
Zhao ◽  
Luo
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Fatty Acid ◽  
High Fat Diet ◽  
Pelteobagrus Fulvidraco ◽  
Mrna Levels ◽  
High Fat ◽  
Primary Hepatocytes ◽  
Yellow Catfish ◽  
Transcriptional Responses

The autophagy-lysosome pathway, which involves many crucial genes and proteins, plays crucial roles in the maintenance of intracellular homeostasis by the degradation of damaged components. At present, some of these genes and proteins have been identified but their specific functions are largely unknown. This study was performed to clone and characterize the full-length cDNA sequences of nine key autolysosome-related genes (vps11, vps16, vps18, vps33b, vps41, lamp1, mcoln1, ctsd1 and tfeb) from yellow catfish Pelteobagrus fulvidraco. The expression of these genes and the transcriptional responses to a high-fat diet and fatty acids (FAs) (palmitic acid (PA) and oleic acid (OA)) were investigated. The mRNAs of these genes could be detected in heart, liver, muscle, spleen, brain, mesenteric adipose tissue, intestine, kidney and ovary, but varied with the tissues. In the liver, the mRNA levels of the nine autolysosome-related genes were lower in fish fed a high-fat diet than those fed the control, indicating that a high-fat diet inhibited formation of autolysosomes. Palmitic acid (a saturated FA) significantly inhibited the formation of autolysosomes at 12 h, 24 h and 48 h incubation. In contrast, oleic acid (an unsaturated FA) significantly induced the formation of autolysosomes at 12 h, but inhibited them at 24 h. At 48 h, the effects of OA incubation on autolysosomes were OA concentration-dependent in primary hepatocytes of P. fulvidraco. The results of flow cytometry and laser confocal observations confirmed these results. PA and OA incubation also increased intracellular non-esterified fatty acid (NEFA) concentration at 12 h, 24 h and 48 h, and influenced mRNA levels of fatty acid binding protein (fabp) and fatty acid transport protein 4 (fatp4) which facilitate FA transport in primary hepatocytes of P. fulvidraco. The present study demonstrated the molecular characterization of the nine autolysosome-related genes and their transcriptional responses to fat and FAs in fish, which provides the basis for further exploring their regulatory mechanism in vertebrates.

scholarly journals Common Bean (Phaseolus vulgaris L.) Flour Can Improve the Gut Microbiota Composition and Function in Mice Fed a High-Fat Diet

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 1159-1159
Author(s):  
Juste Contin Gomes Mariana ◽  
Juliana Soares da Silva ◽  
Andressa Assis ◽  
Elvira Gonzalez de Mejia ◽  
Hilário Cuquetto Mantovani ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Common Bean ◽  
High Fat Diet ◽  
Protein Hydrolysate ◽  
Microbiota Composition ◽  
Gut Health ◽  
High Fat ◽  
Bean Flour ◽  
Cecum Content ◽  
And Function

Abstract Objectives To evaluate the effects of whole flour and protein hydrolysate from common bean on gut health in mice fed a High-Fat Diet. Methods BALB/c adults mice (n = 48) where divided in 4 groups (n = 12 each): normal control (NC standard diet AIN-93M); High-Fat Diet (HFD) only; HFD plus bean flour (HFBF) and HFD plus bean protein hydrolysate (HFPH; 700 mg/kg of body/day). After 9 weeks, the animals were euthanized. Cecum weight was measured and cecum content was collected. Cecum content was used to analyze moisture by gravimetric method, lipids by extraction in Soxhlet apparatus, short chain fatty acids (SCFA) by HPLC and DNA extraction and sequencing of the gut microbiota. Total genomic DNA was extracted from fecal samples and loaded using the Illumina MiSeq platform at Argonne National Laboratory. Data were analyzed by ANOVA and post-hoc of Newman-Kews. Nonparametric and independent samples were submitted to Kruskal-Wallis with a Dunn's multiple comparison test (P < 0.05). Results HFBF increased cecum weight (+69%), moisture (+104.6%) and lipids (+11.5%) in the feces compared to HFD group (P < 0.05), and the Beta diversity was different from HFD. Acetic acid concentration decreased (−37.7%) in cecal content of HFBF group compared to HFD group (P < 0.05), and propionic and butyric acids cecal concentration did not differ (P > 0.05) among experimental groups. The abundance of Bacteroidetes increased and the Firmicutes/Bacteroidetes ratio decreased in the HFBF compared to control groups. The operational taxonomic units (OTUs) enriched by HFBF were mainly assigned to Muribaculaceae family, which show high potential to improve gut health. The functional analysis of the microbiota shown beneficial changes in the host's genetic capacity, especially in the metabolic pathways involved with glucose metabolism. KEGG metabolic pathways involved with starch and sucrose metabolism, as well as the galactose metabolism were enriched in the HFBF group compared to the HFD group (P < 0.05). Conclusions The intake of common bean flour modulates the microbiota composition and abundance of SCFA-producing bacteria, and attenuates the effects of HFD, showing potential to improve gut microbiota composition and function of mice. Funding Sources CNPq, CAPES, Fapemig and Fulbright (Brazil).

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