scholarly journals Chitinase 3-like 1 secreted from cancer-associated fibroblasts promotes tumor angiogenesis via interleukin-8 secretion in colorectal cancer

2021 ◽  
Vol 60 (1) ◽  
Author(s):  
Kaori Watanabe ◽  
Kazuyoshi Shiga ◽  
Anri Maeda ◽  
Shinnosuke Harata ◽  
Takeshi Yanagita ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Tumor Angiogenesis ◽  
Interleukin 8 ◽  
Cancer Associated Fibroblasts

RPS24c Isoform Facilitates Tumor Angiogenesis Via Promoting the Stability of MVIH in Colorectal Cancer

2020 ◽  
Vol 20 (5) ◽  
pp. 388-395 ◽  
Author(s):  
Yue Wang ◽  
Youjun Wu ◽  
Kun Xiao ◽  
Yingjie Zhao ◽  
Gang Lv ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Ribosomal Protein ◽  
Real Time ◽  
Tumor Angiogenesis ◽  
Real Time Pcr ◽  
Molecular Mechanisms ◽  
Migration And Invasion ◽  
Binding Interaction ◽  
Tubule Formation ◽  
The Stability

Background: Colorectal cancer (CRC) is the second leading cause of death worldwide, and distant metastasis is responsible for the poor prognosis in patients with advanced-stage CRC. RPS24 (ribosomal protein S24) as a ribosomal protein, multiple transcript variant encoding different isoforms have been found for this gene. Our previous studies have demonstrated that RPS24 is overexpressed in CRC. However, the mechanisms underlying the role of RPS24 in tumor development have not been fully defined. Methods: Expression of RPS24 isoforms and lncRNA MVIH in CRC tissues and cell lines were quantified by real-time PCR or western blotting assay. Endothelial tube formation assay was performed to determine the effect of RPS24 on tumor angiogenesis. The cell viability of HUVEC was determined by MTT assay, and the migration and invasion ability of HUVEC were detected by transwell assay. PGK1 secretion was tested with a specific ELISA kit. Results: Here, we found that RPS24c isoform was a major contributor to tumor angiogenesis, a vital process in tumor growth and metastasis. Real-time PCR revealed that RPS24c isoform was highly expressed in CRC tissues, while other isoforms are present in both normal and CRC tissues with no statistical difference. Moreover the change of RPS24 protein level is mainly due to the fluctuation of RPS24c. Furthermore, we observed that silencing RPS24c could decrease angiogenesis by inhibiting tubule formation, HUVEC cell proliferation and migration. Additionally, we investigated the molecular mechanisms and demonstrated that RPS24c mRNA interacted with lncRNA MVIH, the binding-interaction enhanced the stability of each other, thereby activated angiogenesis by inhibiting the secretion of PGK1. Conclusion: RPS24c facilitates tumor angiogenesis via the RPS24c/MVIH/PGK1 pathway in CRC. RPS24c inhibition may be a novel option for anti-vascular treatment in CRC.

scholarly journals ING4 suppresses tumor angiogenesis and functions as a prognostic marker in human colorectal cancer

Oncotarget ◽  
2016 ◽  
Vol 7 (48) ◽  
pp. 79017-79031 ◽  
Author(s):  
Yansu Chen ◽  
Yefei Huang ◽  
Pingfu Hou ◽  
Zhe Zhang ◽  
Yafei Zhang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Prognostic Marker ◽  
Tumor Angiogenesis ◽  
Human Colorectal Cancer

scholarly journals Interleukin-34 Enhances the Tumor Promoting Function of Colorectal Cancer-Associated Fibroblasts

Cancers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3537
Author(s):  
Eleonora Franzè ◽  
Antonio Di Grazia ◽  
Giuseppe Sigismondo Sica ◽  
Livia Biancone ◽  
Federica Laudisi ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Proliferation ◽  
Cell Growth ◽  
Cancer Associated Fibroblasts ◽  
Cancer Cell Proliferation ◽  
Fibroblast Growth ◽  
Stromal Compartment ◽  
Large Numbers ◽  
And Migration ◽  
Proliferation And Migration

The stromal compartment of colorectal cancer (CRC) is marked by the presence of large numbers of fibroblasts, termed cancer-associated fibroblasts (CAFs), which promote CRC growth and progression through the synthesis of various molecules targeting the neoplastic cells. Interleukin (IL)-34, a cytokine over-produced by CRC cells, stimulates CRC cell growth. Since IL-34 also regulates the function of inflammatory fibroblasts, we hypothesized that it could regulate the tumor promoting function of colorectal CAFs. By immunostaining and real-time PCR, we initially showed that IL-34 was highly produced by CAFs and to lesser extent by normal fibroblasts isolated from non-tumoral colonic mucosa of CRC patients. CAFs and normal fibroblasts expressed the functional receptors of IL-34. IL-34 induced normal fibroblasts to express α-SMA, vimentin and fibroblast activation protein and enhanced fibroblast growth, thus generating a cellular phenotype resembling that of CAFs. Consistently, knockdown of IL-34 in CAFs with an antisense oligonucleotide (AS) decreased expression of such markers and inhibited cell proliferation. Co-culture of CRC cells with IL-34 AS-treated CAFs supernatants resulted in less cancer cell proliferation and migration. Among CAF-derived molecules known to promote CRC cell growth/migration, only netrin-1 and basic-fibroblast growth factor were induced by IL-34. Data suggest a role for IL-34 in the control of colorectal CAF function.

Abstract 1811: Evaluation of anti-CXCR2 small molecule inhibitors as novel chemotherapy targeting the Interleukin-8 pathway in colorectal cancer

2014 ◽  
Author(s):  
Yinghui Jane E. Huang ◽  
Kevin J. Gaffney ◽  
Ethan Gerdts ◽  
Nicos A. Petasis ◽  
Heinz-Josef Lenz ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Small Molecule ◽  
Small Molecule Inhibitors ◽  
Interleukin 8

Abstract 4330: Activation ofAZIN1RNA editing facilitates and promotes invasive potential of cancer associated fibroblasts in colorectal cancer

2019 ◽  
Author(s):  
Sho Takeda ◽  
Kunitoshi Shigeyasu ◽  
Yoshinaga Okugawa ◽  
Kazuhiro Yoshida ◽  
Yoshiko Mori ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Associated Fibroblasts ◽  
Invasive Potential

Interleukin 8 as predictive factor for response to chemotherapy in colorectal cancer patients

2019 ◽  
pp. 1-6
Author(s):  
Claudia Burz ◽  
Anca Bojan ◽  
Loredana Balacescu ◽  
Vlad-Vasile Pop ◽  
Ciprian Silaghi ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Patients ◽  
Predictive Factor ◽  
Interleukin 8 ◽  
Response To Chemotherapy ◽  
Colorectal Cancer Patients
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