scholarly journals Lentivirus-mediated RNA interference of E2F-1 suppresses Tca8113 cell proliferation

2011 ◽  
Author(s):  
Ning� Chen
Keyword(s):  
Cell Proliferation ◽  
Rna Interference ◽  
Tca8113 Cell

scholarly journals RNA Interference of Human Papillomavirus Type 18 E6 and E7 Induces Senescence in HeLa Cells

2003 ◽  
Vol 77 (10) ◽  
pp. 6066-6069 ◽  
Author(s):  
Allison H. S. Hall ◽  
Kenneth A. Alexander
Keyword(s):  
Cell Proliferation ◽  
Human Papillomavirus ◽  
Rna Interference ◽  
Hela Cells ◽  
Tumor Suppressors ◽  
Small Interfering Rna ◽  
Promote Cell Proliferation ◽  
E6 And E7 ◽  
Cellular Dna ◽  
Interfering Rna

ABSTRACT The human papillomavirus oncoproteins E6 and E7 promote cell proliferation and contribute to carcinogenesis by interfering with the activities of cellular tumor suppressors. We used a small interfering RNA molecule targeting the E7 region of the bicistronic E6 and E7 mRNA to induce RNA interference, thereby reducing expression of E6 and E7 in HeLa cells. RNA interference of E6 and E7 also inhibited cellular DNA synthesis and induced morphological and biochemical changes characteristic of cellular senescence. These results demonstrate that reducing E6 and E7 expression is sufficient to cause HeLa cells to become senescent.

scholarly journals Downregulation of CD147 expression by RNA interference inhibits HT29 cell proliferation, invasion and tumorigenicity in vitro and in vivo

2013 ◽  
Vol 43 (6) ◽  
pp. 1885-1894 ◽  
Author(s):  
RUI LI ◽  
YUQIN PAN ◽  
BANGSHUN HE ◽  
YEQIONG XU ◽  
TIANYI GAO ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Rna Interference ◽  
Ht29 Cell ◽  
Cd147 Expression

scholarly journals Ars2 Links the Nuclear Cap-Binding Complex to RNA Interference and Cell Proliferation

Cell ◽  
2009 ◽  
Vol 138 (2) ◽  
pp. 328-339 ◽  
Author(s):  
Joshua J. Gruber ◽  
D. Steven Zatechka ◽  
Leah R. Sabin ◽  
Jeongsik Yong ◽  
Julian J. Lum ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Rna Interference ◽  
Cap Binding Complex

VEGFR2 inhibition by RNA interference affects cell proliferation, migration, invasion, and response to radiation in Calu-1 cells

2015 ◽  
Vol 18 (2) ◽  
pp. 212-219 ◽  
Author(s):  
Y. Liu ◽  
Y. Qiao ◽  
C. Hu ◽  
L. Liu ◽  
L. Zhou ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Rna Interference

scholarly journals miR-433 Inhibits Neuronal Growth and Promotes Autophagy in Mouse Hippocampal HT-22 Cell Line

2020 ◽  
Vol 11 ◽  
Author(s):  
Chunli Xu ◽  
Qingke Bai ◽  
Chen Wang ◽  
Qiuyu Meng ◽  
Yuming Gu ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Proliferation ◽  
Rna Interference ◽  
Neurodegenerative Diseases ◽  
Cell Line ◽  
Target Genes ◽  
Control Function ◽  
Beclin 1 ◽  
Dependent Manner ◽  
Neuronal Growth

Background: MicroRNAs (miRNAs) have an increasing functional role in some neurodegenerative diseases. Autophagy, the degradation of bulk protein in the cytoplasm, is the quality control function of protein and has a protective role in the survival of neural cells. miR-433 may play a regulatory role in neurodegenerative diseases. Many aspects underlying the mechanism of miR-433 in neural development and neurodegeneration are not clear.Methods: In this study, we established stable cell lines expressing miR-433 by infecting mouse hippocampal neural cell line (HT-22) cells with rLV-miR-433 and the control rLV-miR. Pre-miR-433 expression was analyzed using polymerase chain reaction (PCR). Mature miR-433 expression was measured using quantitative PCR (qPCR). The effect of miR-433 overexpression on cell proliferation was determined using a CCK-8 assay and flow cytometry. RNA interference was used to analyze the function of Cdk12 in mediating the effect of miR-433 on cell proliferation. The effect of miR-433 overexpression on cell apoptosis was determined by flow cytometry. Autophagy-related genes Atg4a, LC3B, and Beclin-1 were determined using qPCR, Western blot, or immunofluorescence. In addition, RNA interference was used to analyze the effect of Atg4a on the induction of autophagy. TargetScan 7.2 was used to predict the target genes of miR-433, and Smad9 was determined using qPCR.Results: Our results indicated that miR-433 increased the expression of Atg4a and induced autophagy by increasing the expression of LC3B-Ⅱ and Beclin-1 in an Atg4a-dependent manner. In addition, miR-433 upregulated the expression of Cdk12 and inhibited cell proliferation in a Cdk12-dependent manner and promoted apoptosis in HT-22 cells under the treatment of 10-hydroxycamptothecin.Conclusion: The results of our study suggest that miR-433 may regulate neuronal growth by promoting autophagy and attenuating cell proliferation. This might be a potential therapeutic intervention in neurodegenerative diseases.

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