miR-140-5p Affects TCC8113 Cell Biological Activity and Matrix Metalloproteinases 9 (MMP-9) in Oral Squamous Cell Carcinoma (OSCC) Cell Line

Whether the miR-140-5p affects the biological activity of Tca8113 cells and MMP-9 in OSCC cell line was explored. Tca8113 cells were divided into Tc group (normal Tca8113 cells), Tm group (Tca8113 cell transfection+miR-140-5p mimic), and Nc group (Tca8113 cell transfection+miR-140-5p-negative control) followed by analysis of MMP-9 expression by western blot, cell migration by Transwell assay, cell viability by MTT method, and apoptosis by flow cytometry. Compared with TC group and NC group, mir-140-5p in TM group was significantly higher (P <0.05), however, the MMP-9 level of TM group was significantly lower (P <0.05).Western blot analysis showed that there was no significant difference (P <0.05) in the comparison of MMP-9 protein expression between TC group and NC group, and MMP-9 expression in the TM group was decreased significantly (P <0.05). MTT assay showed that the cell viability of TM group was increased slowly and lower than that of group (P <0.05). In conclusion, mir-140-5p can induce apoptosis of OSCC cell line Tca8113, which may be achieved by reducing the expression of MMP-9.

Inhibition of HIF-1αAffects Autophagy Mediated Glycosylation in Oral Squamous Cell Carcinoma Cells

Purpose. To validate the function of autophagy with the regulation of hypoxia inhibitor-induced glycosylation in oral squamous cell carcinoma cell.Methods. Human Tca8113 cell line was used to detect autophagy and glycosylation related protein expression by western blotting and immunofluorescence with HIF-1αinhibitor. Short interfering RNA (siRNA) transfection blocked human ATG12 and ATG1.Results. HIF-1αinhibitor PX-478 reduced the amount of LC3-II and LC3-I in Tca8113 cells. PX-478 decreased the expression of O-GlcNAc and OGT and increased OGA expression. The tendency of O-GlcNAc showed a similar pattern to OGT. PX-478 gradually decreased OGT expression in Tca8113 cells. Protein level of O-GlcNAc and OGT increased in ATG12 and ATG1 depletion. The expression of OGT decreased at first and then rose slowly with the treatment of Atg12 and Atg1 siRNA and PX-478 fluctuant. Autophagy affected the stability of OGT when HIF-1αsignaling was blocked.Conclusions. Autophagy reduced by hypoxic stress inhibited. HIF-1αinhibitor decreased glycosylation. OGT became unstable in the absence of autophagy when HIF-1αsignaling was blocked.