Combination of α-fetoprotein mRNA-based detection of hematogenously disseminating hepatocellular carcinoma cells and analysis of cancer cell membrane fluidity is more accurate in screening patients at risk of postoperative recurrence

In recent decades, the use of plants as a natural remedy has been widely applied in traditional medicine and the treatment of various diseases, including cancer. However, in order to confirm the potential benefits of anticancer drug development from natural sources, in-depth screening assessments are necessary. In the present study, we aimed to evaluate the cytotoxic effects of eight medicinal plants against breast carcinoma and hepatocellular carcinoma cell lines. Remarkably, among all the tested plant extracts, Pyracantha angustifolia and Paullinia cupana extracts showed maximum inhibition in the two cancer cell line models, as detected by cell viability assays, but not in normal mammary epithelial cells. Moreover, induction of cell cycle arrest was seen in both cancer cell models after treatment with extracts derived from the fruits of P. angustifolia and the seeds of P. cupana. Phytochemical and antioxidant analyses demonstrated the presence of high phenolic and flavonoid contents, including an increase in 2,2-diphenyl-1-picrylhydrazyl (DPPH) activity. The growth inhibition of human breast carcinoma and hepatocellular carcinoma cells mediated by both extracts appears to be associated with apoptosis and upregulated expression of pro-apoptotic genes (caspase-3, caspase-7, tumor suppressor protein-p53, cytochrome c, poly (ADP-ribose) polymerase, p53 upregulated modulator of apoptosis, and Bcl-2-associated X-protein). Together, these results indicate that P. angustifolia and P. cupana offer a promising approach for the development of anticancer agents. However, further detailed research is required to make these plants applicable for therapeutic use.

Download Full-text

Attenuation of neuroblastoma cell growth by nisin is mediated by modulation of phase behavior and enhanced cell membrane fluidity

Physical Chemistry Chemical Physics ◽

10.1039/c8cp06378h ◽

2019 ◽

Vol 21 (4) ◽

pp. 1980-1987 ◽

Cited By ~ 4

Author(s):

Ashutosh Prince ◽

Anuj Tiwari ◽

Pankaj Ror ◽

Padmani Sandhu ◽

Jyoti Roy ◽

...

Keyword(s):

Cell Growth ◽

Cell Membrane ◽

Antimicrobial Peptide ◽

Phase Behavior ◽

Membrane Fluidity ◽

Cancer Cell ◽

Neuroblastoma Cell ◽

Cancer Cell Growth ◽

Cell Membrane Fluidity

Antimicrobial peptide mediated fluidization of cancer membrane reduces cancer cell growth.

Download Full-text

Anti-Oxidant and Anticancerous Effect of Fomitopsis officinalis (Vill. ex Fr. Bond. et Sing) Mushroom on Hepatocellular Carcinoma Cells In Vitro through NF-kB Pathway

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520621666210608101152 ◽

2021 ◽

Vol 21 ◽

Author(s):

Nyamsambuu Altannavch ◽

Xi Zhou ◽

Md. Asaduzzaman Khan ◽

Ashfaque Ahmed ◽

Shinen Naranmandakh ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Cell Lines ◽

Cancer Cell ◽

Annexin V ◽

Carcinoma Cells ◽

Stress Disorders ◽

Phase Cell ◽

Hepatocellular Carcinoma Cells ◽

The Impact

Background: Fomitopsis officinalis (Vill. ex Fr. Bond. et Sing) is a medicinal mushroom, commonly called ‘Agarikon’, traditionally used to treat cough and asthma in the Mongolian population. Objective: The objective of this study was to examine the significance of biological activity of F. officinalis, and evaluate the antioxidant and anticancer activity of six fractions of F. officinalis residues (Fo1-powder form dissolved in ethanol, Fo2-petroleum ether residue, Fo3-chloroformic, Fo4-ethylacetate, Fo5-buthanolic, and Fo6-water-ethanolic) against hepatocellular carcinoma cells. Methods: We performed in vitro studies of cell proliferation and viability assay, annexin V-FITC/Propidium Iodide assay, and NF-kB signaling pathway by immunoblot analysis. Results: Our findings revealed that all six fractions/extracts have antioxidant activity, and somehow, they exert anticancerous effects against cancer cells. In cancerous cell lines (HepG2 and LO2), Fo3 chloroformic extract promoted the cancer cell apoptosis, cell viability, activated G2/M-phase cell cycle, and selectively induced NF-kB proteins, revealing itself as a novel antitumor extract. Conclusion: This study reports that Fo3-chloroformic extract is rich in antitumor activity; it was previously not investigated in cancer. To study the impact of F. officinalis among natural products to treat/prevent oxidative stress disorders or cancers, further examinations are needed. However, this study assessed only one extract, Fo3-chloroformic, which has a significant impact on cancer cell lines.

Download Full-text