Investigation of probiotic bacteria as dental caries and periodontal disease biotherapeutics

2014 ◽  
Vol 5 (4) ◽  
pp. 447-460 ◽  
Author(s):  
S. Saha ◽  
C. Tomaro-Duchesneau ◽  
L. Rodes ◽  
M. Malhotra ◽  
M. Tabrizian ◽  
...  
Keyword(s):  
Dental Caries ◽  
Periodontal Disease ◽  
Lactobacillus Reuteri ◽  
Bacterial Attachment ◽  
Oral Disease ◽  
Probiotic Bacteria ◽  
Oral Diseases ◽  
Gingival Epithelial Cells ◽  
Health Promoting ◽  
Oral Inflammation

Oral diseases, specifically dental caries and periodontal disease, are characterised by increases in pathogenic microorganisms, increased demineralisation and increased inflammation and levels of inflammatory markers. Despite the therapeutic strategies, oral diseases have elevated prevalence rates. Recent work has demonstrated that probiotic bio-therapeutics can decrease oral pathogen counts, including caries-causing Streptococcus mutans and oral inflammation. The aim of this work was to investigate putative probiotic bacteria, selected for S. mutans inhibition and for their oral health-promoting characteristics. The probiotic bacteria were screened for S. mutans inhibition, probiotic bacteriocin activity, salivary pH modulation, probiotic nutrient (sucrose) competition, probiotic co-aggregation with S. mutans, bacterial attachment to oral epithelial keratinocytes, bacterial nitric oxide production and bacterial antioxidant activity. The results indicate that Lactobacillus reuteri strains NCIMB 701359, NCIMB 701089, NCIMB 702655 and NCIMB 702656 inhibited S. mutans to non-detectable levels (<10 cfu/ml). L. reuteri strains also demonstrated the highest antioxidant capacity of the tested strains (7.73-13.99 µM Trolox equivalents), suggesting their use as both caries and periodontal disease therapeutics. Although Lactobacillus fermentum NCIMB 5221 inhibited S. mutans at lower levels, it significantly buffered the pH (4.18) of saliva containing S. mutans, co-aggregated with S. mutans (10.09%), demonstrated high levels of sucrose consumption (138.11 mM) and successfully attached to gingival epithelial cells (11%). This study identified four L. reuteri strains and one L. fermentum strain to be further investigated as oral disease biotherapeutics.

scholarly journals Systematic Review of the Literature on Dental Caries and Periodontal Disease in Socio-Economically Disadvantaged Individuals

2021 ◽  
Vol 18 (23) ◽  
pp. 12360
Author(s):  
Stefano Cianetti ◽  
Chiara Valenti ◽  
Massimiliano Orso ◽  
Giuseppe Lomurno ◽  
Michele Nardone ◽  
...  
Keyword(s):  
Dental Caries ◽  
Periodontal Disease ◽  
Economically Disadvantaged ◽  
Tooth Loss ◽  
Severe Disease ◽  
Oral Disease ◽  
Tooth Decay ◽  
Oral Diseases ◽  
Policy Makers ◽  
Socially Disadvantaged

Dental caries and periodontal disease represent a health problem and a social cost for the entire population, and in particular for socio-economically disadvantaged individuals who are less resistant to disease. The aim of this review is to estimate the prevalence and severity of the two dental pathologies, caries and periodontal disease, in the different classes of socio-economically disadvantaged subjects and to understand which of them are most affected. A systematic search of the literature was performed in MEDLINE (via PubMed), EMBASE and Web of Science after establishing a suitable search strategy for each database, using keywords related to socio-economically vulnerable classes and health outcomes. Socio-economically disadvantaged individuals are more susceptible to tooth decay and periodontal disease (with relative tooth loss) than non-vulnerable people. Additionally, when multiple vulnerabilities are combined in the same subject, these oral diseases worsen. There is no type of vulnerability more affected by caries and periodontitis than others, since overall they all have severe disease indices. The data from this systematic literature review might be useful for health policy makers looking to allocate more resources and services to socially disadvantaged individuals, resulting in making them more resilient to oral disease due to their social marginalization.

Evaluation of Salivary Interleukin-1ß Levels and Oral Health in Pakistani Male Smokers

2021 ◽  
Vol 15 (6) ◽  
pp. 1158-1163
Author(s):  
S. A. Hamza ◽  
S. Asif ◽  
S. A. H. Bokhari
Keyword(s):  
Oral Health ◽  
Dental Caries ◽  
Low Income ◽  
Smoking Status ◽  
Saliva Sample ◽  
Oral Disease ◽  
Oral Diseases ◽  
Missing Teeth ◽  
Significance Level ◽  
Inflammatory Biomarker

Aim: Smoking induces changes in salivary inflammatory biomarker levels associated with oral diseases. This study status and explored association among salivary Interleukin-1β, oral diseases and smoking. Methods: Data of male smokers of a private institute recruited for a randomized clinical trial is analyzed for this paper. Demographic and systemic information were collected. Oral disease status was examined and saliva sample collected for IL-1β levels. IL-1β levels and other study variables were analyzed with respect to smoking status categorized into smoking years and cigarettes per day. The student's t-test and one-way ANOVA were used for statistical analysis using SPSS version 22) with significance level of p≤0.050. Results: Analysis of baseline data of seventy-eight smokers showed elevated levels of IL-1β with increasing smoking, higher BMI. Smoking was higher among aged, married and low-income individuals. Pearson partial correlation analysis, after controlling age, marital status, education, income, and BMI, demonstrated a positive significant relationship of smoking per day with smoking years; dental caries with missing teeth and calculus; gingivitis with missing teeth and calculus; periodontitis with dental caries, calculus and gingivitis. Conclusion: This analysis demonstrates that levels of IL-1β were raised in smokers, however; there was no association with oral disease parameters. Large studies may be conducted to observe status and association of smoking, oral disease and salivary biomarkers. Keywords: Smoking, Oral Health, IL-1β, Males

Oral Health and Nutrition

2020 ◽  
Author(s):  
Carole. A. Palmer ◽  
Zhangmuge Cheng
Keyword(s):  
Oral Health ◽  
Dental Caries ◽  
Periodontal Diseases ◽  
Oral Disease ◽  
Oral Diseases ◽  
Oral Infections ◽  
Surgeon General ◽  
Optimal Health ◽  
Health And Disease ◽  
Oral Conditions

Oral diseases are among the most prevalent diseases affecting global health. In his report on the crisis in oral disease in America, the Surgeon General warned that one cannot be truly healthy without oral health. Oral health means freedom from all oral health problems; tooth decay (dental caries), periodontal diseases, tooth loss, oral-facial pain, oral cancer and the effects of its treatment, oral infections, craniofacial birth defects and more. The relationships between oral conditions and systemic health and disease are many and synergistic, and most involve dietary and/or systemic nutritional factors. Diet and nutrition can play important roles in the etiology, prevention, and/or management of oral conditions, as they do in overall health and disease. Today, all health professionals and educators need to be aware of and consider oral issues and their possible diet/nutritional implications as a component of optimal health care and education. This review article provides a brief overview of how diet and nutrition impact and are impacted by oral conditions, and offers general guidelines and resources for providing meaningful interventions throughout the life cycle. This review contains 3 figures, 3 tables, and 57 references Key Words: biofilm, cariogenic, dental caries, dental plaque, ECC-early childhood caries, lactobacillus, mucositis, non-cariogenic, periodontal disease, Streptococcus mutans

Prevalence of oral diseases in dogs in Tirana urban area

2016 ◽  
Author(s):  
Haxhi Allmuca ◽  
Péllumb Zalla ◽  
Egon Andoni ◽  
Brunilda Mazari
Keyword(s):  
Periodontal Disease ◽  
Small Animal ◽  
Oral Disease ◽  
Gingival Hyperplasia ◽  
Dental Calculus ◽  
Oral Diseases ◽  
High Prevalence ◽  
Dental Diseases ◽  
The Right ◽  
Veterinary Hospital

The purpose of this study was to determine the frequency of oral diseases in dogs examined at the Small Animal Veterinary Hospital during 2013 - 2014. The dogs underwent a special control for diseases of oral cavity. Site specifications and severity of dental diseases were assessed using modified indexing systems. A total number of 776 dogs, presented at a private Tirana/Albania urban veterinary hospital for different reasons, during this period were analyzed. Analysis by oral disease type revealed 692 dogs or 89.1% dent-gingival disorders. Of these animals, 441 (56.8%) of 776 dogs had periodontal disease; 468 (60.3%) had dental calculus; 241 (31.0%) had teeth loss and 50 (6.4%) had abnormal attrition. Two cases of dogs suffering from tumours, one case with dental caries, two cases with the enamel hypoplasia and one case with gingival hyperplasia, were also observed. Data from this study showed that periodontitis and dental calculus are among the most common diseases prevalent in dogs. There were also observed changes in the deposit of calculus between the upper and lower jaw; with the most calculus deposits observed in the upper jaw. No differences were observed between the right side and the left side of the jaw. Dental calculus index was almost the same between the right side and left side. Frequency of periodontal changes and the degree of inflammation increased with an increase in the age of the dog. Dental calculus was observed at an earlier age in small breeds of dogs. Dental calculus index was lower in large dog breeds compared to the small dog breeds and increased with age. Results of the study showed a high prevalence of oral diseases in dogs and confirmed that periodontal disease is the most common oral disease in dogs.

scholarly journals Protein-Based Salivary Profiles as Novel Biomarkers for Oral Diseases

2018 ◽  
Vol 2018 ◽  
pp. 1-22 ◽  
Author(s):  
Alejandro I. Lorenzo-Pouso ◽  
Mario Pérez-Sayáns ◽  
Susana B. Bravo ◽  
Pía López-Jornet ◽  
María García-Vence ◽  
...  
Keyword(s):  
Dental Caries ◽  
Molecular Biology ◽  
Periodontal Disease ◽  
Global Burden ◽  
Oral Diseases ◽  
Biological Matrix ◽  
Severe Periodontal Disease ◽  
Novel Biomarkers ◽  
Proteins And Peptides ◽  
Untreated Dental Caries

The Global Burden of Oral Diseases affects 3.5 billion people worldwide, representing the number of people affected by the burden of untreated dental caries, severe periodontal disease, and edentulism. Thus, much more efforts in terms of diagnostics and treatments must be provided in the fight of these outcomes. In this sense, recently, the study of saliva as biological matrix has been identified as a new landmark initiative in the search of novel and useful biomarkers to prevent and diagnose these conditions. Specifically, saliva is a rich reservoir of different proteins and peptides and accessible due to recent advances in molecular biology and specially in targeted and unbiased proteomics technologies. Nonetheless, emerging barriers are an obstacle to the study of the salivary proteome in an effective way. This review aims at giving an overall perspective of salivary biomarkers identified in several oral diseases by means of molecular biology approaches.

Protection of probiotic bacteria in a synbiotic matrix following aerobic storage at 4 °C

2012 ◽  
Vol 3 (3) ◽  
pp. 175-187 ◽  
Author(s):  
S. Chaluvadi ◽  
A.T. Hotchkiss Jr. ◽  
J.E. Call ◽  
J.B. Luchansky ◽  
J.G. Phillips ◽  
...  
Keyword(s):  
Lactobacillus Reuteri ◽  
Bifidobacterium Longum ◽  
Storage Conditions ◽  
Probiotic Bacteria ◽  
The Matrix ◽  
Health Promoting ◽  
Pectic Oligosaccharides ◽  
Lactic Acids ◽  
Alginate Matrices ◽  
Alginate Matrix

The survival of single strains of Bifidobacterium breve, Bifidobacterium longum, Lactobacillus acidophilus, and Lactobacillus reuteri was investigated in synbiotics that included 10 mg/ml of fructo-oligosaccharides, inulin and pectic-oligosaccharides in an alginate matrix under refrigerated (4 °C) aerobic storage conditions. When the matrices were cross-linked with calcium (45 mM), 102-103 cfu/ml of L. acidophilus and L. reuteri, and 0-103 cfu/ml of B. breve and B. longum survived refrigerated aerobic storage for 28 days. Following refrigerated storage, acetic (3-9 mM), butyric (0-2 mM), propionic (5-16 mM) and lactic acids (1-48 mM) were produced during the growth of probiotics in BHI broth at 37 °C, suggesting their metabolic activity after storage was stressed. When calcium cross-linking was not used in synbiotics, the matrix remained more gel-like after inoculation when compared to the calcium cross-linked matrix. At least 107 cfu/ml of probiotic bacteria survived after 21 days of storage within these gel-like alginate matrices. Significantly higher levels of B. breve, L. acidophilus and L. reuteri were obtained from the synbiotic matrices supplemented with fructo-oligosaccharides, inulin and pectic-oligosaccharides compared to alginate alone. B. longum survival was the same (~7 logs) in all gel-like synbiotic matrices. These results show that synbiotics protected probiotic bacteria and extended their shelf-life under refrigerated aerobic conditions. Synbiotics represent a viable delivery vehicle for health-promoting bacteria.

scholarly journals How oral infections can influence chronic kidney disease – a review of the literature

2020 ◽  
Vol 23 (1) ◽  
Author(s):  
Carla Cruvinel Pontes ◽  
Usuf ME Chikte
Keyword(s):  
Chronic Kidney Disease ◽  
Oral Health ◽  
Kidney Disease ◽  
Periodontal Disease ◽  
Negative Impact ◽  
The Body ◽  
Oral Disease ◽  
Oral Diseases ◽  
Oral Infections ◽  
Source Of Infection

A healthy mouth is necessary for optimal health and quality of life. However, oral health is often compromised in adults with chronic kidney disease (CKD). The aim of this review was to present the scientific foundations behind the connection between oral diseases and chronic kidney disease (CKD) in adults, discuss common oral conditions and their systemic effects, investigate biological pathways through which oral infections affect the body and provide guidelines for physicians/nephrologists. Prevalence of oral disease is increased in CKD, including periodontal disease, oral mucosal lesions, edentulousness, xerostomia, gingival overgrowth in immunosuppressed patients and potentially caries. There is moderate to strong evidence to support a negative impact of oral infections in CKD, particularly periodontal disease, with systemic inflammation, bacteraemia of oral origin, endothelial function and gut dysbiosis being potential pathways for this interaction. Poor oral health can be a hidden source of infection and has been associated with increased mortality in CKD patients. Elimination of potential foci for oral infections is crucial before renal transplantation. Frequent dental monitoring is crucial for these patients and should be part of a multidisciplinary approach to manage CKD, with special attention to end-stage kidney disease.

scholarly journals Role of cranberry juice in prevention of dental caries and periodontal disease`

2019 ◽  
Author(s):  
Sana Farista ◽  
Akhilesh Jakhete ◽  
Shanin Farista
Keyword(s):  
Dental Caries ◽  
Adverse Effects ◽  
Periodontal Disease ◽  
Dental Plaque ◽  
Oral Diseases ◽  
Cranberry Juice ◽  
Limited Success ◽  
The Many ◽  
Potential Use

Dental caries and Periodontal Disease (periodontitis) are the most common oral diseases. The aetiology of both these diseases can be traced back to the microorganisms in the dental plaque. Over the years numerous anti plaque agents have been developed with limited success and varying degrees of adverse effects. The current research is in the direction of using plant or herbal based products as anti-plaque agent. Out of the many agents studied, cranberry (Vacciniummacrocarpon) has shown maximum potential for use as anti-plaque agent. The current paper aims to review the existing literature on cranberry and its potential use in dentistry in minimizing the prevalence of dental caries and periodontal disease. KEY WORDS: Cranberry Juice, Anti Plaque Agent, Periodontitis, Non Dialyzable Material

scholarly journals Microbiota populations in supragingival plaque, subgingival plaque, and saliva habitats of adult dogs

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Patrícia M. Oba ◽  
Meredith Q. Carroll ◽  
Celeste Alexander ◽  
Helen Valentine ◽  
Amy J. Somrak ◽  
...  
Keyword(s):  
Oral Health ◽  
Oral Cavity ◽  
Periodontal Disease ◽  
Bacterial Species ◽  
Oral Disease ◽  
Oral Microbiota ◽  
Subgingival Plaque ◽  
Oral Diseases ◽  
Supragingival Plaque

Abstract Background Oral diseases are common in dogs, with microbiota playing a prominent role in the disease process. Oral cavity habitats harbor unique microbiota populations that have relevance to health and disease. Despite their importance, the canine oral cavity microbial habitats have been poorly studied. The objectives of this study were to (1) characterize the oral microbiota of different habitats of dogs and (2) correlate oral health scores with bacterial taxa and identify what sites may be good options for understanding the role of microbiota in oral diseases. We used next-generation sequencing to characterize the salivary (SAL), subgingival (SUB), and supragingival (SUP) microbial habitats of 26 healthy adult female Beagle dogs (4.0 ± 1.2 year old) and identify taxa associated with periodontal disease indices. Results Bacterial species richness was highest for SAL, moderate for SUB, and lowest for SUP samples (p < 0.001). Unweighted and weighted principal coordinates plots showed clustering by habitat, with SAL and SUP samples being the most different from one another. Bacteroidetes, Proteobacteria, Firmicutes, Fusobacteria, Actinobacteria, and Spirochaetes were the predominant phyla in all habitats. Paludibacter, Filifactor, Peptostreptococcus, Fusibacter, Anaerovorax, Fusobacterium, Leptotrichia, Desulfomicrobium, and TG5 were enriched in SUB samples, while Actinomyces, Corynebacterium, Leucobacter, Euzebya, Capnocytophaga, Bergeyella, Lautropia, Lampropedia, Desulfobulbus, Enhydrobacter, and Moraxella were enriched in SUP samples. Prevotella, SHD-231, Helcococcus, Treponema, and Acholeplasma were enriched in SAL samples. p-75-a5, Arcobacter, and Pasteurella were diminished in SUB samples. Porphyromonas, Peptococcus, Parvimonas, and Campylobacter were diminished in SUP samples, while Tannerella, Proteocalla, Schwartzia, and Neisseria were diminished in SAL samples. Actinomyces, Corynebacterium, Capnocytophaga, Leptotrichia, and Neisseria were associated with higher oral health scores (worsened health) in plaque samples. Conclusions Our results demonstrate the differences that exist among canine salivary, subgingival plaque and supragingival plaque habitats. Salivary samples do not require sedation and are easy to collect, but do not accurately represent the plaque populations that are most important to oral disease. Plaque Actinomyces, Corynebacterium, Capnocytophaga, Leptotrichia, and Neisseria were associated with higher (worse) oral health scores. Future studies analyzing samples from progressive disease stages are needed to validate these results and understand the role of bacteria in periodontal disease development.

ORAL DISEASES STATUS AND KNOWLEDGE, PRACTICE FOR ORAL CARE OF THE PRIMARY SCHOOL’S PUPILS IN THE CITY AND MOUNTAIN OF THUA THIEN-HUE PROVINCE

2014 ◽  
pp. 177-184
Author(s):  
Tan Tai Tran ◽  
Toai Nguyen ◽  
Ngoc Hoat Luu
Keyword(s):  
Dental Caries ◽  
Dental Care ◽  
Oral Hygiene ◽  
Primary Schools ◽  
Oral Care ◽  
Oral Disease ◽  
Oral Diseases ◽  
Caries Prevalence ◽  
Knowledge And Practice ◽  
Primary Pupils

Background: Oral disease is now the major public health problems. There is few research on the status of dental caries and its relationship to the knowledge and practice of oral hygiene practices among schoolchildren. The objectives of this study were to (1) describe the current status of oral diseases; (2) find out knowledge and practice of oral care and a number of factors related to dental caries. Subjects and Methods: This is a cross-section study what was carried out on 1406 schoolchildren aged 7-11-year- olds attending Grade 1–5 at two primary schools of the Hue city and four primary schools of mountain. Clinical examination and interview questions of knowledge and practice to dental care for all subjects. Results: Dental caries prevalence was 77.6%, the mean dmft/DMFT score was 4.22/1.51; gingivitis, tarta, dental plaque were 33.2%, 31.2%, 55.5% respectively. The questionnaires about oral hygiene, 26.5% of children had not good knowledge, 67.6% had not good practice. Dental caries related with practicing daily oral hygiene (OR = 2.21, p <0.01) and with the knowledge of dental care (OR = 1.44, p <0.05). There is the relationship between knowledge and practice not yet good (OR = 5.25, p <0.01). Conclusions: Oral diseases, particularly dental caries prevalence is still high in primary pupils. It’s necessary for education on knowledge and practice of oral disease prevention for primary pupils. Keywords: Oral disease, primary pupils, oral health KP

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