scholarly journals Heat inactivation partially preserved barrier and immunomodulatory effects of Lactobacillus gasseri LA806 in an in vitro model of bovine mastitis

2021 ◽  
pp. 1-12
Author(s):  
F. Blanchet ◽  
L. Rault ◽  
V. Peton ◽  
Y. Le Loir ◽  
C. Blondeau ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Bovine Mastitis ◽  
Antibiotic Use ◽  
Cytokine Expression ◽  
Heat Inactivation ◽  
Lactobacillus Gasseri ◽  
Bovine Mammary Epithelial Cells ◽  
E Coli ◽  
Vitro Model

Probiotics could help combat infections and reduce antibiotic use. As use of live bacteria is limited in some cases by safety or regulatory concerns, the potential of inactivated bacteria is worth investigating. We evaluated the potential of live and heat-inactivated Lactobacillus gasseri LA806 to counteract Staphylococcus aureus and Escherichia coli infection cycles in an in vitro model of bovine mastitis. We assessed the ability of live and inactivated LA806 to impair pathogen colonisation of bovine mammary epithelial cells (bMECs) and to modulate cytokine expression by pathogen-stimulated bMECs. Live LA806 induced a five-fold decrease in S. aureus adhesion and internalisation (while not affecting E. coli colonisation) and decreased pro-inflammatory cytokine expression by S. aureus-stimulated bMECs (without interfering with the immune response to E. coli). The ability of inactivated LA806 ability to diminish S. aureus colonisation was two-fold lower than that of the live strain, but its anti-inflammatory properties were barely impacted. Even though LA806 effects were impaired after inactivation, both live and inactivated LA806 have barrier and immunomodulatory properties that could be useful to counteract S. aureus colonisation in the bovine mammary gland. As S. aureus is involved in various types of infection, LA806 potential would worth exploring in other contexts.

Growth factor and cytokine expression of human mesenchymal stromal cells is not altered in an in vitro model of tissue damage

Cytotherapy ◽  
2010 ◽  
Vol 12 (7) ◽  
pp. 870-880 ◽  
Author(s):  
Katrin Montzka ◽  
Tobias Führmann ◽  
Jochen Müller-Ehmsen ◽  
Michael Wöltje ◽  
Gary A. Brook
Keyword(s):  
Growth Factor ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Tissue Damage ◽  
In Vitro Model ◽  
Cytokine Expression ◽  
Human Mesenchymal Stromal Cells ◽  
Vitro Model

scholarly journals Obesity and inflammation: reduced cytokine expression due to resveratrol in a human in vitro model of inflamed adipose tissue

2015 ◽  
Vol 6 ◽  
Author(s):  
Ivana Zagotta ◽  
Elitsa Y. Dimova ◽  
Klaus-Michael Debatin ◽  
Martin Wabitsch ◽  
Thomas Kietzmann ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
In Vitro Model ◽  
Cytokine Expression ◽  
Vitro Model

Lactococcus lactis V7 inhibits the cell invasion of bovine mammary epithelial cells by Escherichia coli and Staphylococcus aureus

2015 ◽  
Vol 6 (6) ◽  
pp. 879-886 ◽  
Author(s):  
B. Seridan Assis ◽  
P. Germon ◽  
A.M. Silva ◽  
S. Even ◽  
J.R. Nicoli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Mammary Gland ◽  
Lactococcus Lactis ◽  
Cell Invasion ◽  
Bovine Mastitis ◽  
Antibiotic Use ◽  
Mammary Epithelial ◽  
Bovine Mammary Epithelial Cells ◽  
E Coli

Bovine mastitis, an inflammatory disease of the mammary gland often associated to bacterial infection, is the first cause of antibiotic use in dairy cattle. Because of the risk of antibioresistance emergence, alternative non-antibiotic strategies are needed to prevent or to cure bovine mastitis and reduce the antibiotic use in veterinary medicine. In this work, we investigated Lactococcus lactis V7, a strain isolated from the mammary gland, as a probiotic option against bovine mastitis. Using bovine mammary epithelial cell (bMEC) culture, and two representative strains for Escherichia coli and for Staphylococcus aureus, two major mastitis pathogens, we investigated L. lactis V7 ability to inhibit cell invasion (i.e. adhesion and internalization) of these pathogens into bMEC. L. lactis V7 ability to modulate the production of CXCL8, a key chemokine IL-8 responsible for neutrophil influx, in bMEC upon challenge with E. coli was investigated by an ELISA dosage of CXCL8 in bMEC culture supernatants. We showed that L. lactis V7 inhibited the internalisation of both E. coli and S. aureus strains into bMEC, whereas it inhibited the adhesion of only one out of the two S. aureus strains and of none of the E. coli strains tested. Investigation of the bMEC immune response showed that L. lactis V7 alone induced a slight increase in CXCL8 production in bMEC and that it increased the inflammatory response in bMEC challenged with the E. coli strains. Altogether these features of L. lactis V7 make it a potential promising candidate for a probiotic prevention strategy against bovine mastitis.

Leukocyte antibacterial functions are not impaired by perfluorocarbon exposure in vitro

2008 ◽  
Vol 295 (1) ◽  
pp. L134-L142 ◽  
Author(s):  
Dirk Haufe ◽  
Eva Koenigshausen ◽  
Lilla Knels ◽  
Martina Wendel ◽  
Sebastian N. Stehr ◽  
...  
Keyword(s):  
Host Defense ◽  
In Vitro Model ◽  
Polymorphonuclear Neutrophils ◽  
Human Neutrophils ◽  
Intracellular Uptake ◽  
E Coli ◽  
Transmission Electron ◽  
Membrane Bound ◽  
Vitro Model

Application of liquid, aerosolized, and vaporized perfluorocarbons (PFC) in acute lung injury has shown anti-inflammatory effects. Although this may be beneficial in states of pulmonary hyperinflammation, it also could increase susceptibility to nosocomial lung infection. We hypothesized that PFC impair cellular host defense and therefore investigated in an in vitro model the influence of perfluorohexane (PFH) on crucial mechanisms of bacterial elimination in human neutrophils and monocytes. Using scanning and transmission electron microscopy, we could show membrane-bound and ingested PFH particles that morphologically did not alter adherence and phagocytosis of Escherichia coli or leukocyte viability. The amount of adherent and phagocytosed bacteria as determined by flow cytometry was not influenced in cells only pretreated with PFH for 1 and 4 h. When PFH was present during E. coli challenge, bacterial adherence was decreased in polymorphonuclear neutrophils, but respective intracellular uptake was not impaired and was even significantly promoted in monocytes. Overall, E. coli-induced respiratory burst capacity was not reduced by PFH. Our findings provide evidence that key functions of innate host defense are not compromised by PFH treatment in vitro.

scholarly journals Sophorolipid treatment decreases inflammatory cytokine expression in an in vitro model of experimental sepsis

2006 ◽  
Vol 20 (4) ◽  
Author(s):  
Cathy M Mueller ◽  
Yin‐yao Lin ◽  
Domenico Viterbo ◽  
Joelle Pierre ◽  
Shirley A Murray ◽  
...  
Keyword(s):  
Inflammatory Cytokine ◽  
In Vitro Model ◽  
Cytokine Expression ◽  
Experimental Sepsis ◽  
Vitro Model

scholarly journals Nanodiamonds facilitate killing of intracellular uropathogenic E. coli in an in vitro model of urinary tract infection pathogenesis

PLoS ONE ◽  
2018 ◽  
Vol 13 (1) ◽  
pp. e0191020 ◽  
Author(s):  
Janaki Kannan Iyer ◽  
Alexia Dickey ◽  
Parvaneh Rouhani ◽  
Anil Kaul ◽  
Nirmal Govindaraju ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
In Vitro Model ◽  
E Coli ◽  
Vitro Model

scholarly journals Precision-cut bovine udder slices (PCBUS) as an in-vitro-model of an early phase of infection of bovine mastitis

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Viviane Filor ◽  
Monique Petry ◽  
Jessica Meißner ◽  
Manfred Kietzmann
Keyword(s):  
Infectious Diseases ◽  
Early Phase ◽  
In Vitro Model ◽  
Antimicrobial Agents ◽  
Bovine Mastitis ◽  
Therapeutic Approaches ◽  
New Therapeutic Approaches ◽  
Pharmacological Interactions ◽  
Vitro Model

Abstract Background The aim of this study was to establish precision-cut bovine udder slices (PCBUS) as an in-vitro-model to investigate pathophysiological processes in the early phase of mastitis in order to have the possibility to investigate new therapeutic approaches for the treatment of such udder inflammation in later studies. Furthermore, this model should contribute to substitute in-vivo-experiments. Bovine mastitis is one of the most common and costly infectious diseases in the dairy industry, which is largely associated with the use of antimicrobial agents. Given this problem of antimicrobial resistance, it is essential to step up research into bacterial infectious diseases. Thus, the transfer of the in-vitro-model of precision-cut tissue slices to the bovine udder enables broad research into new therapeutic approaches in this area and can also be used to address issues in basic research or the characterisation of complex pathophysiological processes. Results A stimulation with LPS, PGN or the combination of both substances (LPS:PGN) demonstrates the ability of the PCBUS to react with a significant secretion of IL-1ß, TNF-α and PGE2. Conclusion The slices represent an instrument for investigating pharmacological interactions with udder tissue, which can be useful for studies on pharmacological questions and the understanding of complex pathophysiological processes of infection and inflammation.

scholarly journals Precision-Cut Bovine Udder Slices (PCBUS) as an in-vitro-model of an early phase of infection of bovine mastitis

2020 ◽  
Author(s):  
Viviane Filor ◽  
Monique Petry ◽  
Jessica Meißner ◽  
Manfred Kietzmann
Keyword(s):  
Infectious Diseases ◽  
Early Phase ◽  
In Vitro Model ◽  
Antimicrobial Agents ◽  
Bovine Mastitis ◽  
Therapeutic Approaches ◽  
New Therapeutic Approaches ◽  
Pharmacological Interactions ◽  
Vitro Model

Abstract Background: The aim of this study was the establishment of precision-cut bovine udder slices (PCBUS) as an in-vitro -model to investigate pathophysiological processes in the early phase of mastitis in order to have the possibility to investigate new therapeutic approaches for the treatment of such udder inflammation in later studies. Furthermore, this model should contribute to substitute in-vivo -experiments. Bovine mastitis is one of the most common and costly infectious diseases in the dairy industry, which is largely associated with the use of antimicrobial agents. Given this problem of antimicrobial resistance, it is essential to step up research into bacterial infectious diseases. Thus, the transfer of the in-vitro -model of precision-cut tissue slices to the bovine udder enables broad research into new therapeutic approaches in this area and can also be used to address issues in basic research or the characterization of complex pathophysiological processes. Results: A stimulation with LPS, PGN or the combination of both substances (LPS:PGN) demonstrated the ability of the PCBUS to react with a significant secretion of IL-1ß, TNF-α and PGE 2 . Conclusion: The slices represent an instrument for investigating pharmacological interactions with udder tissue, which can be useful for studies on pharmacological questions and the understanding of complex pathophysiological processes of infection and inflammation.

Impact of bacterial species and baseline resistance on fosfomycin efficacy in urinary tract infections

2019 ◽  
Vol 75 (4) ◽  
pp. 988-996 ◽  
Author(s):  
Iain J Abbott ◽  
Jordy Dekker ◽  
Elke van Gorp ◽  
Rixt A Wijma ◽  
Merel N Raaphorst ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Bacterial Species ◽  
Clinical Isolates ◽  
Infection Model ◽  
Limit Of Quantification ◽  
E Coli ◽  
Mutant Prevention Concentration ◽  
Vitro Model ◽  
Tract Infections

Abstract Objectives To assess the antibacterial effects of a single 3 g oral fosfomycin dose on Escherichia coli and Klebsiella pneumoniae clinical isolates within a dynamic bladder infection model. Methods An in vitro model simulating dynamic urinary fosfomycin concentrations was used. Target fosfomycin exposure (Cmax = 1984 mg/L and Tmax = 7.5 h) was validated by LC-MS/MS. Pharmacodynamic responses of 24 E. coli and 20 K. pneumoniae clinical isolates were examined (fosfomycin MIC ≤0.25–128 mg/L). Mutant prevention concentration (MPC), fosfomycin heteroresistance, fosfomycin resistance genes and fosA expression were examined. Pathogen kill and emergence of high-level resistance (HLR; MIC >1024 mg/L) were quantified. Results Following fosfomycin exposure, 20 of 24 E. coli exhibited reductions in bacterial counts below the lower limit of quantification without regrowth, despite baseline fosfomycin MICs up to 128 mg/L. Four E. coli regrew (MIC = 4–32 mg/L) with HLR population replacement. At baseline, these isolates had detectable HLR subpopulations and MPC >1024 mg/L. All E. coli isolates were fosA negative. In contrast, 17 of 20 K. pneumoniae regrew post exposure, 6 with emergence of HLR (proportion = 0.01%–100%). The three isolates without regrowth did not have a detectable HLR subpopulation after dynamic drug-free incubation. All K. pneumoniae had MPC >1024 mg/L and were fosA positive. WGS analysis and fosA expression failed to predict fosfomycin efficacy. Conclusions E. coli and K. pneumoniae isolates demonstrate discrepant responses to a single fosfomycin dose in a dynamic bladder infection in vitro model. Treatment failure against E. coli was related to an HLR subpopulation, not identified by standard MIC testing. Activity against K. pneumoniae appeared limited, regardless of MIC testing, due to universal baseline heteroresistance.

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