Toxicokinetic study and oral bioavailability of deoxynivalenol in turkey poults, and comparative biotransformation between broilers and turkeys

2015 ◽  
Vol 8 (4) ◽  
pp. 533-539 ◽  
Author(s):  
M. Devreese ◽  
G. Antonissen ◽  
N. Broekaert ◽  
T. De Mil ◽  
S. De Baere ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Oral Administration ◽  
Oral Bioavailability ◽  
Broiler Chickens ◽  
High Resolution Mass Spectrometry ◽  
Major Metabolite ◽  
Turkey Poults ◽  
Absolute Oral Bioavailability ◽  
Resolution Mass

The aim of present study was to reveal the toxicokinetic properties and absolute oral bioavailability of deoxynivalenol (DON) in turkey poults. Six turkey poults were administered this Fusarium mycotoxin per os and intravenously in a two-way cross-over design. Based on non-compartmental analysis, DON was absorbed rapidly (Tmax= 0.57 h) but incomplete, as the oral bioavailability was only 20.9%. DON was rapidly eliminated as well, both after oral (T1/2elimination PO=0.86 h) as well as intravenous (IV) (T1/2elimination IV = 0.62 h) administration. Furthermore, semi-quantitative analysis using high-resolution mass spectrometry revealed that DON-3α-sulphate is the major metabolite of DON in turkeys after IV as well as oral administration, with DON-3α-sulphate/DON ratios between 1.3-12.6 and 32.4-140.8 after IV and oral administration, respectively. Glucuronidation of DON to DON-3α-glucuronide is a minor pathway in turkey poults, with DON-3α-glucuronide/DON ratios between 0.009-0.065 and 0.020-0.481 after IV and oral administration, respectively. Only trace amounts of other metabolites were found including 10-DON-sulphonate, de-epoxydeoxynivalenol and 10-de-epoxydeoxynivalenol-sulphonate. In addition, a similar two-way cross-over study was performed in three broiler chickens, in order to compare the biotransformation of DON in both poultry species. High-resolution mass spectrometry revealed that DON-3α-sulphate was the major metabolite of DON in broiler chickens as well, with DON-3α-sulphate/DON ratios between 243-453 and 1,365-29,624 after IV and oral administration, respectively. These ratios indicate that broiler chickens metabolise DON even more extensively to the sulphate conjugate compared to turkey poults. Only trace amounts of other metabolites were detected in broiler chickens. In conclusion, it can be stated that the toxicokinetic behaviour of DON in broiler chickens and turkey poults is comparable (low absolute oral bioavailability, rapid absorption and elimination, extensive biotransformation to DON-3α-sulphate), however, relative differences in DON-3α-sulphate/DON ratios exist between both species which might explain the hypothesised difference in sensitivity of both poultry species to DON.

scholarly journals Metabolic Profile, Bioavailability and Toxicokinetics of Zearalenone-14-Glucoside in Rats after Oral and Intravenous Administration by Liquid Chromatography High-Resolution Mass Spectrometry and Tandem Mass Spectrometry

2019 ◽  
Vol 20 (21) ◽  
pp. 5473 ◽  
Author(s):  
Feifei Sun ◽  
Haiguang Tan ◽  
Yanshen Li ◽  
Marthe De Boevre ◽  
Sarah De Saeger ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Resolution ◽  
Oral Administration ◽  
Intravenous Administration ◽  
Metabolic Profile ◽  
Glucuronic Acid ◽  
High Resolution Mass Spectrometry ◽  
Tandem Mass ◽  
Resolution Mass

Zearalenone-14-glucoside (ZEN-14G), a key modified mycotoxin, has attracted a great deal of attention due to the possible conversion to its free form of zearalenone (ZEN) exerting toxicity. In this study, the toxicokinetics of ZEN-14G were investigated in rats after oral and intravenous administration. The plasma concentrations of ZEN-14G and its major five metabolites were quantified using a validated liquid chromatography tandem mass spectrometry (LC-MS/MS) method. The data were analyzed via non-compartmental analysis using software WinNonlin 6.3. The results indicated that ZEN-14G was rapidly hydrolyzed into ZEN in vivo. In addition, the major parameters of ZEN-14G following intravenous administration were: area under the plasma concentration–time curve (AUC), 1.80 h·ng/mL; the apparent volume of distribution (VZ), 7.25 L/kg; and total body clearance (CL), 5.02 mL/h/kg, respectively. After oral administration, the typical parameters were: AUC, 0.16 h·ng/mL; VZ, 6.24 mL/kg; and CL, 4.50 mL/h/kg, respectively. The absolute oral bioavailability of ZEN-14G in rats was about 9%, since low levels of ZEN-14G were detected in plasma, which might be attributed to its extensive metabolism. Therefore, liquid chromatography high-resolution mass spectrometry (LC-HRMS) was adopted to clarify the metabolic profile of ZEN-14G in rats’ plasma. As a result, eight metabolites were identified in which ZEN-14-glucuronic acid (ZEN-14GlcA) had a large yield from the first time-point and continued accumulating after oral administration, indicating that ZEN-14-glucuronic acid could serve a potential biomarker of ZEN-14G. The obtained outcomes would prompt the accurate safety evaluation of ZEN-14G.

Exploring the Active Compounds of Traditional Mongolian Medicine Agsirga in Intervention of Novel Coronavirus (2019-nCoV) Based on HPLC-Q-Exactive-MS/MS and Molecular Docking Method

2020 ◽  
Author(s):  
Jie Cheng ◽  
Yuchen Tang ◽  
Baoquan Bao ◽  
Ping Zhang
Keyword(s):  
Mass Spectrometry ◽  
Molecular Docking ◽  
High Resolution ◽  
Mass Spectra ◽  
High Resolution Mass Spectrometry ◽  
Structural Formula ◽  
Active Compounds ◽  
High Resolution Mass ◽  
Q Exactive ◽  
Resolution Mass

<p><a></a><a></a><a></a><a><b>Objective</b></a>: To screen all compounds of Agsirga based on the HPLC-Q-Exactive high-resolution mass spectrometry and find potential inhibitors that can respond to 2019-nCoV from active compounds of Agsirga by molecular docking technology.</p> <p><b>Methods</b>: HPLC-Q-Exactive high-resolution mass spectrometry was adopted to identify the complex components of Mongolian medicine Agsirga, and separated by the high-resolution mass spectrometry Q-Exactive detector. Then the Orbitrap detector was used in tandem high-resolution mass spectrometry, and the related molecular and structural formula were found by using the chemsipider database and related literature, combined with precise molecular formulas (errors ≤ 5 × 10<sup>−6</sup>) , retention time, primary mass spectra, and secondary mass spectra information, The fragmentation regularities of mass spectra of these compounds were deduced. Taking ACE2 as the receptor and deduced compounds as the ligand, all of them were pretreated by discover studio, autodock and Chem3D. The molecular docking between the active ingredients and the target protein was studied by using AutoDock molecular docking software. The interaction between ligand and receptor is applied to provide a choice for screening anti-2019-nCoV drugs.</p> <p><b>Result</b>: Based on the fragmentation patterns of the reference compounds and consulting literature, a total of 96 major alkaloids and stilbenes were screened and identified in Agsirga by the HPLC-Q-Exactive-MS/MS method. Combining with molecular docking, a conclusion was got that there are potential active substances in Mongolian medicine Agsirga which can block the binding of ACE2 and 2019-nCoV at the molecular level.</p>

Determination of quaternary ammonium compounds in food products by the method of ultra-performance liquid chromatography/high resolution mass-spectrometry

2020 ◽  
Vol 86 (8) ◽  
pp. 23-31
Author(s):  
V. G. Amelin ◽  
D. S. Bolshakov
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Quaternary Ammonium ◽  
High Resolution Mass Spectrometry ◽  
Food Products ◽  
Quaternary Ammonium Compounds ◽  
High Resolution Mass ◽  
Ammonium Compounds ◽  
Resolution Mass

The goal of the study is developing a methodology for determination of the residual amounts of quaternary ammonium compounds (QAC) in food products by UHPLC/high-resolution mass spectrometry after water-acetonitrile extraction of the determined components from the analyzed samples. The identification and determination of QAC was carried out on an «UltiMate 3000» ultra-high-performance liquid chromatograph (Thermo Scientific, USA) equipped with a «maXis 4G» high-resolution quadrupole-time-of-flight mass spectrometric detector and an ion spray «ionBooster» source (Bruker Daltonics, Germany). Samples of milk, cheese (upper cortical layer), dumplings, pork, chicken skin and ground beef were used as working samples. Optimal conditions are specified for chromatographic separation of the mixture of five QAC, two of them being a mixture of homologues with a linear structure (including isomeric forms). The identification of QAC is carried out by the retention time, exact mass of the ions, and coincidence of the mSigma isotopic distribution. The limits for QAC detection are 0.1 – 0.5 ng/ml, the determination limits are 1 ng/ml for aqueous standard solutions. The determinable content of QAC in food products ranges within 1 – 100 ng/g. The results of analysis revealed the residual amount of QAC present in all samples, which confirms data of numerous sources of information about active use of QAC-based disinfectants in the meat and dairy industry. The correctness of the obtained results is verified by introduction of the additives in food products at a level of 10 ng/g for each QAC. The relative standard deviation of the analysis results does not exceed 0.18. The duration of the analysis is 30 – 40 min.

Measuring Exposome Associated with Health

2020 ◽  
pp. 46-50
Keyword(s):  
Mass Spectrometry ◽  
Remote Sensing ◽  
High Resolution ◽  
Biological Effect ◽  
High Resolution Mass Spectrometry ◽  
The Body ◽  
Ecological Environment ◽  
Special Issue ◽  
The Life Course ◽  
Resolution Mass

The concept of exposome has received increasing discussion, including the recent Special Issue of Science –"Chemistry for Tomorrow's Earth,” about the feasibility of using high-resolution mass spectrometry to measure exposome in the body, and tracking the chemicals in the environment and assess their biological effect. We discuss the challenges of measuring and interpreting the exposome and suggest the survey on the life course history, built and ecological environment to characterize the sample of study, and in combination with remote sensing. They should be part of exposomics and provide insights into the study of exposome and health.

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