The Effects of Root Aquatic Extract of Salvia staminea on Neuronal Density of Alpha Motoneurons in Spinal Cord Anterior Horn after Sciatic Nerve Compression in Rats

Background: Increase of SIRT1 expression inhibit the increase of activated Caspase 3 expression in acute phase of traumatic brain injury (TBI). However, the SIRT1 expression on rat spinal cord in acute phase after peripheral nerve injury was unknown. Objective: To reveal SIRT1and activated Caspase 3 expression on rat spinal cord in acute phase after sciatic nerve injury and to reveal the correlation between SIRT1 and activated Caspase 3 expression after sciatic nerve injury. Method: Thirty male Wistar rats aged 3 months were divided into sham operated and crush injury group. Termination was performing at days 3, 7 and 14. Lumbar spinal cord segments 4-5 (VTh12-VL1 high) embedded in paraffin blocks. Imunohistochemistry staining was performed using antibody of SIRT1 nuclear marker and activated Caspase 3. Observations were performed by two double blinded observers in 400x magnification. The data were analyzed using unpaired t-test and Pearson correlation. All data were processed using statistical analysis with confidence interval (CI) 95% and limit of significance (p-values)<0.05. Results: SIRT1 expression on the anterior horn was higher compared with control at days 7 and 14 (p<0.05), but no differences were found on posterior horn at all termination days(p>0.05). Activated Caspase 3 expression on the anterior horn was higher at all termination days(p<0.05) and at day 7 on posterior horn (p<0.05). Thereis a positive correlation between SIRT1 and activated Caspase 3 expression on anterior horn at day 7 (p<0.05). Conclusion: SIRT1 expression on the anterior horn was higher compared with the control at days 7 and 14. Activated Caspase 3 expression on the anterior horn was higher on all termination days and at day 7 on posterior horn. There is a positive correlation between SIRT1 and activated Caspase 3 expression on anterior horn on day 7. Bangladesh Journal of Medical Science Vol.18(1) 2019 p.50-56

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Sciatic nerve injury alters the spatial arrangement of neurons and glial cells in the anterior horn of the spinal cord

Neural Regeneration Research ◽

10.4103/1673-5374.257539 ◽

2019 ◽

Vol 14 (10) ◽

pp. 1833 ◽

Cited By ~ 4

Author(s):

Mohammad-Amin Abdollahifar ◽

RezaMastery Farahni ◽

Ali Rashidiani-Rashidabadi ◽

MohammadHassan Heidari ◽

Ensieh Sajadi ◽

...

Keyword(s):

Spinal Cord ◽

Sciatic Nerve ◽

Nerve Injury ◽

Glial Cells ◽

Spatial Arrangement ◽

Sciatic Nerve Injury ◽

Anterior Horn

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Neuroprotection effects of ethyl acetate and n-butanol fractions of the hydroalcoholic extract of Tanacetum bodjnordens on sciatic nerve compression in male rats

Journal of Birjand University of Medical Sciences ◽

10.32592/jbirjandunivmedsci.2021.28.2.102 ◽

2021 ◽

pp. 120-128

Keyword(s):

Sciatic Nerve ◽

Ethyl Acetate ◽

Aqueous Phase ◽

Nerve Compression ◽

Male Rats ◽

Thigh Muscle ◽

Neuroprotective Effects ◽

Neuronal Density ◽

Treatment Groups ◽

Neuron Cell Body

Background and Aims: When a neuronal axon is damaged, it returns to the neuron cell body and destroys it. Tanacetum bodjnordens as antioxidant and anti-apoptotic effects. This study aimed to determine the neuroprotective effects of ethyl acetate and n-butanol and hydroalcoholic extracts of Tanacetum bodjnordens on sciatic nerve compression in male rats. Materials and Methods: In this experimental study, 36 male Wistar rats weighing 200-250 g were randomly divided into 6 groups )n=6). In the control group, the right thigh muscle of the rats was split after the anesthetization of the rats, while in the compression and treatment groups, the sciatic nerve was compressed for 60 seconds. The plant extract was injected intraperitoneally on the day of compression and seven days later. After 28 days, samples were taken from the lumbar spinal cord subsequent to performing the perfusion method. Afterward, 7-μm serial sections were prepared and stained using toluidine blue stain after tissue passage. Eventually, the neuronal density of rats in the six groups was compared. Results: Based on the results, the neuronal density in the compression group decreased significantly compared to controls and showed a significant increase in the hydroalcoholic, n-butanol, and aqueous phase treatment groups compared to that in the compression group (P<0.001). Conclusion: According to the results, it seems that Tanacetum bodjnordens leaf extract has neuroprotective effects that promote the regeneration process in damaged neurons and these effects are higher in the aqueous phase fraction.

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Faculty Opinions recommendation of Bortezomib treatment produces nocifensive behavior and changes in the expression of TRPV1, CGRP, and substance P in the rat DRG, spinal cord, and sciatic nerve.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.726421087.793519748 ◽

2016 ◽

Author(s):

Miriam Freimer

Keyword(s):

Spinal Cord ◽

Substance P ◽

Sciatic Nerve ◽

Nocifensive Behavior ◽

Bortezomib Treatment

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Micro- and Astroglia Activity in the Spinal Cord Ventrolateral Nucleus Sciatic Nerve Injury in Rats

Cell and Tissue Biology ◽

10.1134/s1990519x20040100 ◽

2020 ◽

Vol 14 (4) ◽

pp. 263-269

Author(s):

A. A. Starinets ◽

E. L. Egorova ◽

A. A. Tyrtyshnaia ◽

I. V. Dyuisen ◽

A. N. Baryshev ◽

...

Keyword(s):

Spinal Cord ◽

Sciatic Nerve ◽

Nerve Injury ◽

Sciatic Nerve Injury ◽

Ventrolateral Nucleus

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SUR1, newly expressed in astrocytes, mediates neuropathic pain in a mouse model of peripheral nerve injury

Molecular Pain ◽

10.1177/17448069211006603 ◽

2021 ◽

Vol 17 ◽

pp. 174480692110066

Author(s):

Orest Tsymbalyuk ◽

Volodymyr Gerzanich ◽

Aaida Mumtaz ◽

Sanketh Andhavarapu ◽

Svetlana Ivanova ◽

...

Keyword(s):

Spinal Cord ◽

Neuropathic Pain ◽

Sciatic Nerve ◽

Peripheral Nerve ◽

Dorsal Horn ◽

Nerve Injury ◽

Peripheral Nerve Injury ◽

Thermal Sensitivity ◽

Gradual Improvement ◽

Pain Behaviors

Background Neuropathic pain following peripheral nerve injury (PNI) is linked to neuroinflammation in the spinal cord marked by astrocyte activation and upregulation of interleukin 6 (IL -6 ), chemokine (C-C motif) ligand 2 (CCL2) and chemokine (C-X-C motif) ligand 1 (CXCL1), with inhibition of each individually being beneficial in pain models. Methods Wild type (WT) mice and mice with global or pGfap-cre- or pGFAP-cre/ERT2-driven Abcc8/SUR1 deletion or global Trpm4 deletion underwent unilateral sciatic nerve cuffing. WT mice received prophylactic (starting on post-operative day [pod]-0) or therapeutic (starting on pod-21) administration of the SUR1 antagonist, glibenclamide (10 µg IP) daily. We measured mechanical and thermal sensitivity using von Frey filaments and an automated Hargreaves method. Spinal cord tissues were evaluated for SUR1-TRPM4, IL-6, CCL2 and CXCL1. Results Sciatic nerve cuffing in WT mice resulted in pain behaviors (mechanical allodynia, thermal hyperalgesia) and newly upregulated SUR1-TRPM4 in dorsal horn astrocytes. Global and pGfap-cre-driven Abcc8 deletion and global Trpm4 deletion prevented development of pain behaviors. In mice with Abcc8 deletion regulated by pGFAP-cre/ERT2, after pain behaviors were established, delayed silencing of Abcc8 by tamoxifen resulted in gradual improvement over the next 14 days. After PNI, leakage of the blood-spinal barrier allowed entry of glibenclamide into the affected dorsal horn. Daily repeated administration of glibenclamide, both prophylactically and after allodynia was established, prevented or reduced allodynia. The salutary effects of glibenclamide on pain behaviors correlated with reduced expression of IL-6, CCL2 and CXCL1 by dorsal horn astrocytes. Conclusion SUR1-TRPM4 may represent a novel non-addicting target for neuropathic pain.

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Some Points in the Histology of Lymphogenous and Hæmatogenous Toxic Lesions of the Spinal Cord

Journal of Mental Science ◽

10.1192/bjp.54.226.560 ◽

1908 ◽

Vol 54 (226) ◽

pp. 560-561

Author(s):

David Orr ◽

R. G. Rows

Keyword(s):

Spinal Cord ◽

Central Nervous System ◽

Nervous System ◽

Sciatic Nerve ◽

Morphological Type ◽

Broth Culture ◽

Anatomical Distribution ◽

Tabes Dorsalis ◽

The Central Nervous System ◽

The Brain

At a quarterly meeting of this Association held last year at Nottingham, we showed the results of our experiments with toxins upon the spinal cord and brain of rabbits. Our main conclusion was, that the central nervous system could be infected by toxins passing up along the lymph channels of the perineural sheath. The method we employed in our experiments consisted in placing a celloidin capsule filled with a broth culture of an organism under the sciatic nerve or under the skin of the cheek; and we invariably found a resulting degeneration in the spinal cord or brain, according to the situation of the capsule. These lesions we found to be identical in morphological type and anatomical distribution with those found in the cord of early tabes dorsalis and in the brain and cord of general paralysis of the insane. The conclusion suggested by our work was that these two diseases, if toxic, were most probably infections of lymphogenous origin.

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