scholarly journals A case of mistaken identity: Genetic and morphological evidence for the presence of Redeye Bass in the Verde River, Arizona

2021 ◽  
Author(s):  
Matthew Valente ◽  
Catherine Benson ◽  
Matthew Chmiel ◽  
Matthew Lewis ◽  
Eric Peatman ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Lateral Line ◽  
River System ◽  
Smallmouth Bass ◽  
Morphological Evidence ◽  
Genetic Sequencing ◽  
Nadh Dehydrogenase Subunit 2 ◽  
Verde River ◽  
Black Bass ◽  
Redeye Bass

We report genetic and morphological evidence for the presence of Redeye Bass Micropterus coosae, in the Verde River of Arizona, previously thought to be Smallmouth Bass Micropterus dolomieu. We performed meristic measurements on 15 individuals sampled from the Upper Verde River Wildlife Area, Yavapai County, Arizona. Meristic data for lateral line scales, scales above lateral line, and scales below lateral line were all consistent with Redeye Bass and not Smallmouth Bass. We analyzed mitochondrial and nuclear genetic data to determine if one of the black bass (Genus Micropterus) species historically introduced to the Verde River was Redeye Bass and that they persist in the system. We extracted DNA from fin clips of five individuals for phylogenetic analysis of the NADH dehydrogenase subunit 2 (ND2) mitochondrial gene and for analysis of nuclear DNA using a diagnostic Single Nucleotide Polymorphism (SNP) panel. Results of the ND2 genetic sequencing and phylogenetic analysis indicated that these fish likely originated from native Redeye Bass stock from the Coosa River system of Alabama, Georgia, and Tennessee. Similarly, nuclear SNP data from the five individuals collected from the Verde River aligned with Redeye Bass reference genotypes based on STRUCTURE analysis. These results support the hypothesis that at least one of the introductions of black bass in Arizona’s Verde River founded a previously unrecognized population of Redeye Bass.  Further work is needed to determine the extent of the Redeye Bass presence in Arizona, whether Smallmouth Bass are also present in the Verde River system, and if hybridization of Redeye Bass and other black basses is occurring.

Black Bass Diversity: Multidisciplinary Science for Conservation

2015 ◽  
Keyword(s):  
River System ◽  
Smallmouth Bass ◽  
Savannah River ◽  
River Systems ◽  
Nadh Dehydrogenase Subunit 2 ◽  
Black Bass ◽  
Apalachicola River ◽  
Cryptic Biodiversity ◽  
Redeye Bass ◽  
River Drainages

<em>Abstract</em>.—Shoal basses are a cryptic clade composed of <em>Micropterus </em>spp. restricted to the Apalachicola River system and three southeastern Atlantic slope river drainages in the southeastern United States. This reciprocally monophyletic clade includes the Shoal Bass <em>M. cataractae </em>(endemic to the Apalachicola River system), the Chattahoochee Bass <em>M. chattahoochae</em>, and two undescribed forms from the Altamaha, Ogeechee, and Savannah River drainages. Members of the shoal bass clade can be distinguished from all other species of <em>Micropterus </em>basses using 20 diagnostic characters (characteristic attributes) found in mitochondrial DNA (NADH dehydrogenase subunit 2) gene sequences. Each member of the clade additionally possesses unique characteristic attributes, which along with morphological and meristic characters can be used to diagnose this cryptic biodiversity. Biologists and managers have previously regarded the shoal basses in the Chattahoochee, Savannah, Altamaha and Ogeechee River systems as belonging to a single taxon synonymous with the Redeye Bass <em>M. coosae</em>, which is endemic to the Mobile River drainage. With these and previous analyses (including description of the Shoal Bass), we now recognize that what was once considered a single taxon actually comprises seven species, each of which is endemic to a single southeastern drainage. Recognizing and documenting the actual diversity of <em>Micropterus </em>spp. provides important information for managers who may wish to avoid stocking or translocations that could compromise the genetic integrity of native bass populations. Introductions of nonnative basses, including Alabama Bass <em>M. henshalli</em>, Spotted Bass <em>M. punctulatus</em>, and Smallmouth Bass <em>M. dolomieu </em>currently threaten the integrity of native shoal bass species in streams of the Chattahoochee, Altamaha, Ogeechee, and Savannah River systems.

Black Bass Diversity: Multidisciplinary Science for Conservation

2015 ◽  
Keyword(s):  
River Basin ◽  
River System ◽  
Smallmouth Bass ◽  
Genetic Integrity ◽  
Isolated Populations ◽  
High Incidence ◽  
Conservation Actions ◽  
Black Bass ◽  
Flint River ◽  
Chattahoochee River

<em>Abstract</em>.—Shoal Bass <em>Micropterus cataractae</em> are native only to the Apalachicola–Chattahoochee–Flint River system of Georgia, Alabama, and Florida. Shoal Bass are vulnerable to extinction as a result of population fragmentation and introduction of nonnative <em>Micropterus</em> species. We assessed the genetic integrity of four isolated populations of Shoal Bass in the upper Chattahoochee River basin (above Lake Lanier, in Big Creek, below Morgan Falls Dam, and at Cochran Shoals) and sought to determine rates of hybridization with nonnative, illegally stocked Smallmouth Bass <em>M. dolomieu</em> and Alabama Bass <em>M. henshalli</em>. Collected specimens were evaluated using 10 highly polymorphic microsatellite markers and Bayesian population assignment software. Shoal Bass below Morgan Falls Dam exhibited high levels of hybridization with Smallmouth Bass and possibly Alabama Bass, coupled with a high incidence of the nonnative parental forms. Rates of hybridization among specimens from Cochran Shoals were similarly high, although fewer nonnative parental forms were observed. Among the 30 tested, three specimens morphologically identified as Shoal Bass in Big Creek were either pure Smallmouth Bass or hybrids of Shoal Bass and Smallmouth Bass. The only Shoal Bass population not significantly impacted by congeneric hybridization was found upstream of Lake Lanier. Conservation actions aimed at reducing or eliminating nonnative black bass species and their hybrids could maintain the genetic integrity of Shoal Bass populations in the upper Chattahoochee River basin and lessen their risk of extirpation.

Morphological evidence for paraphyly of Holopothrips Hood (Thysanoptera: Phlaeothripidae), a Neotropical genus of gall-inducing thrips

2021 ◽  
pp. 1-24
Author(s):  
Mariana F. Lindner ◽  
Augusto Ferrari ◽  
Adriano Cavalleri
Keyword(s):  
Phylogenetic Analysis ◽  
Host Plants ◽  
Morphological Diversity ◽  
Morphological Characters ◽  
Bootstrap Support ◽  
Morphological Evidence ◽  
Diverse Group ◽  
The Matrix ◽  
Internal Relations ◽  
Evolutionary Story

Abstract Holopothrips is a diverse group of thrips associated to galls in the Neotropics, with a variety of host plants and wide morphological diversity. Relationships to other Neotropical groups have been proposed, but are still untested, and the monophyly of the genus remains doubtful. Here, we perform a phylogenetic analysis of Holopothrips, based on morphological characters. A total of 87 species were included in the matrix and eight analyses were carried out, but all of them failed to recover Holopothrips as a monophyletic grouping. Bremer and Bootstrap support values were low, and the topologies varied among all analyses, with uncertain internal relations for the ingroup. These results indicate that the relationships for Holopothrips species, and the proposed related genera, are more complex than previously reported; and morphological characters may not be enough to recover the evolutionary story within this group. We also discuss the influences of different character coding, continuous characters and weighting schemes in our results.

Black Bass Diversity: Multidisciplinary Science for Conservation

2015 ◽  
Keyword(s):  
United States ◽  
South Carolina ◽  
Native Species ◽  
Natural Habitat ◽  
Conservation Strategies ◽  
Southern United States ◽  
Physical Habitat ◽  
River Watershed ◽  
Black Bass ◽  
Redeye Bass

<em>Abstract</em>.—Rivers and streams of the southern United States contain more than 1,800 aquatic species, 500 of which are regionally endemic. At present, 34% of the fish species and 90% of the mussel species in peril nationwide are found in these systems. Declines in these imperiled species are due to many factors, including hydrologic alteration, degraded water quality, loss of instream and watershed connectivity, physical habitat degradation, and the negative effects of nonindigenous species (e.g., predation on, competition with, and hybridization with native forms). In addition, this situation is exacerbated through human population growth, competing water demands, land-use changes, and other interrelated issues. If unchecked, these issues will likely continue to contribute to the imperilment and loss of native species in the region. Of the nine described species and subspecies of black bass, six are endemic to the southern United States: Guadalupe Bass <em>Micropterus treculii</em>, Shoal Bass <em>M. cataractae</em>, Redeye Bass <em>M. coosae</em>, Florida Bass <em>M. floridanus</em>, Alabama Bass <em>M. henshalli</em>, and Suwannee Bass <em>M. notius</em>. In addition, undescribed species and subspecies also exist and all are in need of conservation measures to prevent them from becoming imperiled. In an effort to focus and coordinate actions to support the long-term persistence of endemic black bass populations, local, state, and federal agencies, universities, nongovernmental organizations, and corporations from across the region joined with the National Fish and Wildlife Foundation to form the Native Black Bass Initiative (NBBI). The NBBI provides regional conservation strategies, objectives, and targets to restore and preserve functional processes in those watersheds that support natural habitat conditions and sustainable populations of endemic black bass and other native fishes of the region. Initial actions implemented through the NBBI focus on addressing the conservation needs of Guadalupe Bass in streams of the Edwards Plateau ecoregion of Texas, Redeye Bass in the Savannah River watershed of Georgia and South Carolina, and Shoal Bass populations in the Apalachicola River watershed of Alabama, Florida, and Georgia.

Black Bass Diversity: Multidisciplinary Science for Conservation

2015 ◽  
Keyword(s):  
Population Dynamics ◽  
Management Strategies ◽  
Smallmouth Bass ◽  
Micropterus Dolomieu ◽  
Spawning Period ◽  
Sampling Gear ◽  
Gray Literature ◽  
Black Bass ◽  
Effective Conservation ◽  
Conservation And Management

<em>Abstract</em>.—We reviewed the published and gray literature associated with Neosho Smallmouth Bass <em>Micropterus dolomieu velox</em> and the genetically distinct Ouachita lineage. Substantial interstream variation appears to occur among these populations, particularly related to age. The Neosho subspecies is more abundant, grows faster, and lives longer than the genetically distinct Ouachita lineage. Recruitment is highly variable among streams for both populations and appears to be related to some undescribed aspects of hydrology but also likely reflect bias due to sampling gear. Information on annual and seasonal trends is lacking for the Neosho subspecies and the Ouachita lineages, particularly as related to the spawning period. Conservation efforts for these lineages might benefit from agencies partnering to achieve goals that extend beyond a particular agency’s responsibilities and state boundaries. Recognition of spatial and temporal considerations, combined with a better understanding of the population dynamics as related to abundance, growth, mortality and reproduction, would benefit the creation of more effective conservation and management strategies for genetically distinct populations of Smallmouth Bass <em>M. dolomieu</em>.

Black Bass Diversity: Multidisciplinary Science for Conservation

2015 ◽  
Keyword(s):  
Economic Impact ◽  
Impact Analysis ◽  
Habitat Degradation ◽  
Economic Value ◽  
Smallmouth Bass ◽  
Economic Impact Analysis ◽  
Reservoir Fisheries ◽  
Central Texas ◽  
Black Bass ◽  
Preferred Species

<em>Abstract</em>.—The Guadalupe Bass <em>Micropterus treculii</em> is a central Texas endemic black bass species occurring only in streams and rivers draining the Edwards Plateau ecoregion. It is designated the state fish of Texas and provides a popular sport fishery. In addition to being a popular sport fish, it is listed as a species of special concern due to habitat degradation and hybridization with Smallmouth Bass <em>M. dolomieu</em>. Past socioeconomic surveys of Texas black bass anglers have focused primarily on reservoir fisheries while little is known about fishing patterns, economic impact, and preferences of river and stream anglers. A Web-based open-access survey was used to determine fishing characteristics, assess attitudes and quantify the economic impact of anglers fishing rivers and streams in a 24-county region of Texas from August 20, 2011 to December 20, 2012, with a focus on anglers who specifically fished for Guadalupe Bass. A total of 700 respondents participated in the survey. More than half of respondents were paddlers targeting black bass, and 42% specifically fished for Guadalupe Bass on their trips. An additional 34% of anglers listed black bass species, which included Guadalupe Bass as their preferred species. Similar to previous surveys of Texas river and stream anglers, access was identified as the largest impediment to the future maintenance and improvement of river and stream fishing. Based on 563 surveys used in the economic impact analysis, using IMPLAN (Impact Analysis for Planning) Professional version 2 (Minnesota IMPLAN Group, Minneapolis), an estimated US$74,182,080 in direct angler expenditures was spent on fishing trips to the study region, resulting in a total economic impact (including indirect and induced impacts) of $71,552,492 and 776 full-time jobs. These findings indicate the economic value of river and stream angling to the Texas economy.

Reassignment of 'Triplophysa' jiarongensis (Cypriniformes: Nemacheilidae) to Oreonectes Günther, 1868

Zootaxa ◽  
2019 ◽  
Vol 4565 (3) ◽  
pp. 398 ◽  
Author(s):  
XUAN LIU ◽  
HUAIQING DENG ◽  
ZHIJIN LIU ◽  
JIANG ZHOU
Keyword(s):  
Phylogenetic Analysis ◽  
Short Distance ◽  
River System ◽  
Genetic Distances ◽  
Molecular Evidence ◽  
Karst Cave ◽  
Bayesian Phylogenetic Analysis ◽  
As Species ◽  
Secondary Sexual Dimorphism ◽  
Underground River

Specimens identified as Oreonectes jiarongensis (Cypriniformes: Nemacheilidae) were collected from a karst cave in Jiarong Town and Banzhai Township, Libo County, Guizhou, China. Several lines of morphological and molecular evidence suggested that this species was similar to species of Oreonectes Günther 1868 and not closely related to species of Triplophysa Rendal, 1933. The anterior and posterior nostrils of 'Triplophysa' jiarongensis were separated by a short distance, and there was no secondary sexual dimorphism in male specimens. The Bayesian phylogenetic analysis based on cytochrome b recovered 'T.' jiarongensis in a well-supported clade with Oreonectes daqikongensis, O. shuilongensis, O. furcocaudalis and O. platycephalus, sister to the Triplophysa clade. In addition, the genetic distances between 'T.' jiarongensis and species of Oreonectes were low (O. daqikongensis: 0.114; O. shuilongensis: 0.106; O. platycephalus: 0.180), while distances to species of Triplophysa were higher (T. dorsalis: 0.233; T. yarkandensis: 0.282). Therefore, we reassign 'T.' jiarongensis to Oreonectes. Based on this result, it is now clear that species of both Oreonectes and Triplophysa inhabit the same underground river system in Guizhou. However, as species of Oreonectes are only known from the southern part of Guizhou, it may be that O. jiarongensis inhabits the most northerly part of the range of this genus. 

Revision of the status of Saurolophus (Hadrosauridae) from California, USA

2010 ◽  
Vol 47 (11) ◽  
pp. 1417-1426 ◽  
Author(s):  
Phil R. Bell ◽  
David C. Evans
Keyword(s):  
Phylogenetic Analysis ◽  
Sierra Nevada ◽  
Sister Taxon ◽  
Morphological Evidence ◽  
Magmatic Arc ◽  
The Status ◽  
Previous Assignment ◽  
Geographic Setting

The occurrence of Saurolophus from the Moreno Formation (late Maastrichtian) of California is investigated and an incomplete, poorly preserved, skull (LACM/CIT 2852) is described. The skull lacks the braincase (including the frontals) and much of the nasals, and the preserved portions are crushed or plastically deformed, which makes anatomical interpretations difficult. A preserved midline fragment of the conjoined nasals suggests that it lacked a gryposaur-like “Roman nose”, but the nature of the crest, if present, is impossible to determine with certainty. A phylogenetic analysis places this specimen as either the sister taxon of Saurolophus or as the sister taxon to a clade comprising Edmontosaurus and Anatotitan . There is no compelling morphological evidence to support the previous assignment of LACM/CIT 2852 to Saurolophus rather than to Edmontosaurus, and its poor preservation prevents positive assignment to any taxon below Hadrosaurinae indet. Given its geographic setting and morphological uncertainties, it is also possible that this specimen represents a separate taxon, but more material is needed to clarify the identity of the Moreno hadrosaurine. LACM/CIT 2852 does, however, provide evidence that Maastrichtian hadrosaurines ranged west of the Sierra Nevada magmatic arc, in an area where dinosaur diversity is poorly known.

scholarly journals Guidelines for Sanger sequencing and molecular assay monitoring

2020 ◽  
Vol 32 (6) ◽  
pp. 767-775
Author(s):  
Beate M. Crossley ◽  
Jianfa Bai ◽  
Amy Glaser ◽  
Roger Maes ◽  
Elizabeth Porter ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
American Association ◽  
Sequence Data ◽  
Epidemiologic Studies ◽  
Molecular Assay ◽  
Sequencing Data ◽  
Genetic Sequencing ◽  
Laboratory Services ◽  
Diagnostic Applications ◽  
Laboratory Technology

Genetic sequencing, or DNA sequencing, using the Sanger technique has become widely used in the veterinary diagnostic community. This technology plays a role in verification of PCR results and is used to provide the genetic sequence data needed for phylogenetic analysis, epidemiologic studies, and forensic investigations. The Laboratory Technology Committee of the American Association of Veterinary Laboratory Diagnosticians has prepared guidelines for sample preparation, submission to sequencing facilities or instrumentation, quality assessment of nucleic acid sequence data performed, and for generating basic sequencing data and phylogenetic analysis for diagnostic applications. This guidance is aimed at assisting laboratories in providing consistent, high-quality, and reliable sequence data when using Sanger-based genetic sequencing as a component of their laboratory services.

New crayfish species records from the Sipsey Fork drainage, including Lewis Smith Reservoir (Alabama, USA): native or introduced species?

2015 ◽  
Vol 21 (1) ◽  
pp. 17-32
Author(s):  
Susan B. Adams ◽  
Craig Roghair ◽  
Colin Krause ◽  
Melvin L. Warren ◽  
J. Allison Cochran ◽  
...  
Keyword(s):  
Introduced Species ◽  
Native Species ◽  
River System ◽  
Orconectes Virilis ◽  
Transition Zones ◽  
Intestinal Contents ◽  
Black Bass ◽  
Community Changes ◽  
Black Warrior River ◽  
Crayfish Species

Abstract As part of a study of aquatic faunal community changes along riverine-lacustrine transition zones upstream of Lewis Smith Reservoir in northwest Alabama, USA, we collected crayfish from 60 sites in the Sipsey Fork, Brushy Creek, and selected tributaries (Black Warrior River system). After finding two unexpected and possibly-introduced crayfish species, we expanded our investigation of crayfish distributions to include crayfish obtained from stomachs of black bass (Micropterus spp.) caught at seven sites in the reservoir. To explore what crayfish species were in the drainage historically, we examined museum databases as well as stomach and intestinal contents of a variety of preserved fishes that were caught in the Sipsey Fork and Brushy Creek drainages upstream of the reservoir in the early 1990’s. Of the seven crayfish species collected, one, Orconectes (Procericambarus) sp. nr ronaldi, was not previously reported from Alabama, and another, O. lancifer, was not reported from the Black Warrior River system prior to the study. Three are known or possibly introduced species. Upstream of the reservoir, the native species Cambarus obstipus, C. striatus, and O. validus were common. The same three species were found in fish collected in the 1990’s. Orconectes perfectus was found only in the reservoir but may be native to the drainage. Orconectes lancifer was in the reservoir and in stream reaches influenced by the reservoir. Evidence points to O. lancifer being introduced in the drainage, but this is uncertain. Orconectes sp. nr ronaldi was found in a relatively small portion of Brushy Creek and its tributaries, in both flowing and impounded habitats, and may be introduced. Orconectes virilis is introduced in Alabama and was found only in stomachs of fish collected in the reservoir.

Sign in / Sign up

Export Citation Format

Share Document