scholarly journals Effect of silk fibroin biomaterial coating on cell viability and intestinal adhesion of probiotic bacteria

2021 ◽  
Author(s):  
Gicheol Kwon ◽  
Bohye Heo ◽  
Mi Jin Kwon ◽  
Insu Kim ◽  
Jaeryang Chu ◽  
...  
Keyword(s):  
Cell Viability ◽  
Silk Fibroin ◽  
Probiotic Bacteria ◽  
Intestinal Adhesion

scholarly journals Chitosan hydrogel/silk fibroin/Mg(OH)2 nanobiocomposite as a novel scaffold with antimicrobial activity and improved mechanical properties

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Reza Eivazzadeh-Keihan ◽  
Fateme Radinekiyan ◽  
Hooman Aghamirza Moghim Aliabadi ◽  
Sima Sukhtezari ◽  
Behnam Tahmasebi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Cell Viability ◽  
Silk Fibroin ◽  
Analytical Techniques ◽  
Structural Features ◽  
Mechanical Tests ◽  
Chitosan Hydrogel ◽  
Biological Capacity ◽  
Ft Ir ◽  
Novel Scaffold

AbstractHerein, a novel nanobiocomposite scaffold based on modifying synthesized cross-linked terephthaloyl thiourea-chitosan hydrogel (CTT-CS hydrogel) substrate using the extracted silk fibroin (SF) biopolymer and prepared Mg(OH)2 nanoparticles was designed and synthesized. The biological capacity of this nanobiocomposite scaffold was evaluated by cell viability method, red blood cells hemolytic and anti-biofilm assays. According to the obtained results from 3 and 7 days, the cell viability of CTT-CS/SF/Mg(OH)2 nanobiocomposite scaffold was accompanied by a considerable increment from 62.5 to 89.6% respectively. Furthermore, its low hemolytic effect (4.5%), and as well, the high anti-biofilm activity and prevention of the P. aeruginosa biofilm formation confirmed its promising hemocompatibility and antibacterial activity. Apart from the cell viability, blood biocompatibility, and antibacterial activity of CTT-CS/SF/Mg(OH)2 nanobiocomposite scaffold, its structural features were characterized using spectral and analytical techniques (FT-IR, EDX, FE-SEM and TG). As well as, given the mechanical tests, it was indicated that the addition of SF and Mg(OH)2 nanoparticles to the CTT-CS hydrogel could improve its compressive strength from 65.42 to 649.56 kPa.

scholarly journals A Silk Fibroin Based Hydration Accelerator for Root Canal Filling Materials

Polymers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 994
Author(s):  
Ching-Shuan Huang ◽  
Sung-Chih Hsieh ◽  
Nai-Chia Teng ◽  
Wei-Fang Lee ◽  
Poonam Negi ◽  
...  
Keyword(s):  
Cell Viability ◽  
Silk Fibroin ◽  
Root Canal ◽  
Setting Time ◽  
Testing Machine ◽  
Mineral Trioxide Aggregate ◽  
Filling Material ◽  
Root Canal Filling ◽  
Initial Setting Time

Mineral trioxide aggregate (MTA) is widely used in various dental endodontic applications such as root-end filling, furcal perforation repair, and vital pulp therapy. In spite of many attempts to improve handling properties and reduce the discoloration of MTA, the ideal root canal filling material has yet to be fully developed. The objective of this study was to investigate the setting time, mechanical properties, and biocompatibility of MTA set by a silk fibroin solution. A 5 wt% silk fibroin (SF) solution (a novel hydration accelerant) was used to set SavDen® MTA and ProRoot® white MTA (WMTA). Changes in setting time, diametral tensile strength (DTS), material crystallization, in vitro cell viability, and cell morphology were assessed by Vicat needle measurement, a universal testing machine, scanning electron microscopy (SEM), and WST-1 assay, respectively. The initial setting time of ProRoot® MTA and SavDen® MTA experienced a drastic decrease of 83.9% and 42.1% when deionized water was replaced by 5 wt% SF solution as the liquid phase. The DTS of SavDen® MTA showed a significant increase after set by the SF solution in 24 h. A human osteoblast-like cell (MG-63)-based WST-1 assay revealed that both ProRoot® MTA and SavDen® MTA hydrated using SF solution did not significantly differ (p > 0.05) in cell viability. MG-63 cells with pseudopodia attachments and nuclear protrusions represent a healthier and more adherent status on the surface of MTA when set with SF solution. The results suggest that the 5 wt% SF solution may be used as an alternative hydration accelerant for MTA in endodontic applications.

scholarly journals Cell Viability and Functionality of Probiotic Bacteria in Dairy Products

2011 ◽  
Vol 2 ◽  
Author(s):  
Gabriel Vinderola ◽  
Ana Binetti ◽  
Patricia Burns ◽  
Jorge Reinheimer
Keyword(s):  
Cell Viability ◽  
Dairy Products ◽  
Probiotic Bacteria

scholarly journals Effect of Silk Fibroin on Cell Viability in Electrospun Scaffolds of Polyethylene Oxide

Polymers ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 451
Author(s):  
Gabriela Carrasco-Torres ◽  
Manuel Valdés-Madrigal ◽  
Verónica Vásquez-Garzón ◽  
Rafael Baltiérrez-Hoyos ◽  
Eduard De la Cruz-Burelo ◽  
...  
Keyword(s):  
Cell Viability ◽  
Silk Fibroin ◽  
Healing Process ◽  
Breast Implants ◽  
Human Keratinocytes ◽  
Prosthesis Implantation ◽  
Electrospun Scaffolds ◽  
Angle Measurements ◽  
The Matrix ◽  
Poly Ethylene

In this study, a coating from electrospun silk fibroin was performed with the aim to modify the surface of breast implants. We evaluated the effect of fibroin on polymeric matrices of poly (ethylene oxide) (PEO) to enhance cell viability, adhesion, and proliferation of HaCaT human keratinocytes to enhance the healing process on breast prosthesis implantation. We electrospun six blends of fibroin and PEO at different concentrations. These scaffolds were characterized by scanning electron microscopy, contact angle measurements, ATR-FTIR spectroscopy, and X-ray diffraction. We obtained diverse network conformations at different combinations to examine the regulation of cell adhesion and proliferation by modifying the microstructure of the matrix to be applied as a potential scaffold for coating breast implants. The key contribution of this work is the solution it provides to enhance the healing process on prosthesis implantation considering that the use of these PEO–fibroin scaffolds reduced (p < 0.05) the amount of pyknotic nuclei. Therefore, viability of HaCaT human keratinocytes on PEO–fibroin matrices was significantly improved (p < 0.001). These findings provide a rational strategy to coat breast implants improving biocompatibility.

Effect of Thai Silk Sericin and its Extraction Methods on L929 Mouse Fibroblast Cell Viability

2010 ◽  
Vol 93-94 ◽  
pp. 385-388 ◽  
Author(s):  
Piyanut Thitiwuthikiat ◽  
Pornanong Aramwit ◽  
Sorada Kanokpanont
Keyword(s):  
Cell Viability ◽  
Bombyx Mori ◽  
Silk Fibroin ◽  
Extraction Methods ◽  
Proliferation Rate ◽  
Population Doubling ◽  
Population Doubling Time ◽  
Mouse Fibroblasts ◽  
L929 Mouse ◽  
Cells Cultured

Sericin, one of the two main proteins of silk cocoon besides fibroin, has been wildly used as ingredient in cosmetic products and nutrition supplements; however there are considerable controversial reports on its toxicity to cells and its advantages. This work aimed to investigate cell biocompatibility of sericin both in the systems of blended silk fibroin/sericin and pure sericin films using L929 mouse fibroblasts. The effect of concentration of commercial heat extracted sericin on cell viability was first investigated in the system of silk fibroin/sericin (F/S) films using 2 types of silk fibroin, Bombyx mori Nangnoi Srisaket 1 and Bombyx mori Jul 1/1. For both types of silk fibroin, it was found the lower cell number attached and proliferated on the blended F/S films at all sericin concentrations, compared to that on glass and pure fibroin. However, proliferation rate of cells cultured on the blended F/S films was similar to that of cells cultured on glass and pure fibroin films, as confirmed by population doubling time (PDT). Cytotoxicity of sericin extracted from 4 different methods including heat, acid, alkali and urea treatments was further studied in this work in the system of pure sericin films. It could be seen that acid and urea extracted sericin films showed high percentage of cell attachment at 92% and 88%, respectively. However, number of cells proliferated on all sericin films after 48 h culture was not significantly different. This indicated that L929 cells had different proliferation rate when cultured on different types of sericin films. Among 4 extraction methods, the PDT of cells proliferated on urea extracted sericin film was lowest (27 h) and also lower than that of the blended F/S films. This study suggested that sericin extracted by urea treatment could enhance proliferation rate of L929 mouse fibroblasts.

Observation of Proliferation and Attachment of Three Different Cell Types on Nano-Fibrous Silk Film & Scaffold

2007 ◽  
Vol 342-343 ◽  
pp. 85-88 ◽  
Author(s):  
Hyun Sook Baek ◽  
Young Hwan Park ◽  
Ki Chang Seok ◽  
Jong Chul Park ◽  
Don Kyun Rah
Keyword(s):  
Alkaline Phosphatase ◽  
Cell Viability ◽  
Phosphatase Activity ◽  
Silk Fibroin ◽  
Alkaline Phosphatase Activity ◽  
Mtt Assay ◽  
Cell Attachment ◽  
Three Dimensional ◽  
Cell Types ◽  
Different Cell Types

Attachment and viability of different cell types(fibrioblast, chondrocyte and osteoblast ) was observed on two forms of silk (mat & Three-dimensional scaffolds). The osteoblasts behaviors cultured on silk mat were significantly higher than that found on 3-D silk fibroin scaffold (3-D SF scaffold). In the MTT assay, the cell viability of fibroblasts, chondrocyte and osteoblasts seeded on 2-D nanofiber mat was (2-D mat) significantly higher than that found on 3-D SF scaffold. Similar result could be seen from SEM observation and cell attachment study. However, alkaline phosphatase activity was significantly increased on 3-D SF scaffold than on2-D nanofiber

Photo-Driven Synthesis of Anisotropic Gold Nanoparticles Using Silk Fibroin—Cell Viability Activities in Lymphocyte and Jurkat Cancer Cells

2020 ◽  
Vol 10 (4) ◽  
pp. 864-875
Author(s):  
R. Ranjana ◽  
N. Parushuram ◽  
K. S. Harisha ◽  
B. Narayana ◽  
Y. Sangappa
Keyword(s):  
Gold Nanoparticles ◽  
Cell Viability ◽  
Cancer Cells ◽  
Silk Fibroin ◽  
Anisotropic Gold Nanoparticles

Cytoprotective Effect of Tocotrienol-Rich Fraction and α-Tocopherol Vitamin E Isoforms Against Glutamate-Induced Cell Death in Neuronal Cells

2014 ◽  
Vol 84 (3-4) ◽  
pp. 0140-0151 ◽  
Author(s):  
Thilaga Rati Selvaraju ◽  
Huzwah Khaza’ai ◽  
Sharmili Vidyadaran ◽  
Mohd Sokhini Abd Mutalib ◽  
Vasudevan Ramachandran ◽  
...  
Keyword(s):  
Vitamin E ◽  
Cell Viability ◽  
Neuronal Cells ◽  
Positive Control ◽  
Post Treatment ◽  
Mitochondrial Activity ◽  
Before And After ◽  
Pre Treatment ◽  
Tocotrienol Rich Fraction ◽  
Major Mediator

Glutamate is the major mediator of excitatory signals in the mammalian central nervous system. Extreme amounts of glutamate in the extracellular spaces can lead to numerous neurodegenerative diseases. We aimed to clarify the potential of the following vitamin E isomers, tocotrienol-rich fraction (TRF) and α-tocopherol (α-TCP), as potent neuroprotective agents against glutamate-induced injury in neuronal SK-N-SH cells. Cells were treated before and after glutamate injury (pre- and post-treatment, respectively) with 100 - 300 ng/ml TRF/α-TCP. Exposure to 120 mM glutamate significantly reduced cell viability to 76 % and 79 % in the pre- and post-treatment studies, respectively; however, pre- and post-treatment with TRF/α-TCP attenuated the cytotoxic effect of glutamate. Compared to the positive control (glutamate-injured cells not treated with TRF/α-TCP), pre-treatment with 100, 200, and 300 ng/ml TRF significantly improved cell viability following glutamate injury to 95.2 %, 95.0 %, and 95.6 %, respectively (p < 0.05).The isomers not only conferred neuroprotection by enhancing mitochondrial activity and depleting free radical production, but also increased cell viability and recovery upon glutamate insult. Our results suggest that vitamin E has potent antioxidant potential for protecting against glutamate injury and recovering glutamate-injured neuronal cells. Our findings also indicate that both TRF and α-TCP could play key roles as anti-apoptotic agents with neuroprotective properties.

Sign in / Sign up

Export Citation Format

Share Document