<i>In Vitro</i> Dissolution Behavior of Biphasic Tricalcium Phosphate Composite Powders Composed of α-Tricalcium Phosphate and β-Tricalcium Phosphate

Biphasic tricalcium phosphate (BTCP) powders composed of α-tricalcium phosphate (α-TCP) and β-tricalcium phosphate (β-TCP) were prepared using amorphous calcium phosphate (ACP) precursor after heat treatment at 800oC. The in vitro dissolution behavior of the powders was examined after soaked in 0.1M NaAc-HAc buffer solution for different times. It was revealed that the Ca2+ and PO4 3- concentration, and pH value of the BTCP-soaked solution are higher than those of the α-TCP- and β-TCP-soaked solutions. The dissolution behavior of BTCP powders was explained. The specific dissolution behavior of BTCP powders can widen the biodegradation range of calcium phosphate family.

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Preparation, characterization and in vitro dissolution behavior of porous biphasic α/β-tricalcium phosphate bioceramics

Materials Science and Engineering C ◽

10.1016/j.msec.2015.11.040 ◽

2016 ◽

Vol 59 ◽

pp. 1007-1015 ◽

Cited By ~ 19

Author(s):

Lu Xie ◽

Haiyang Yu ◽

Yi Deng ◽

Weizhong Yang ◽

Li Liao ◽

...

Keyword(s):

Tricalcium Phosphate ◽

In Vitro Dissolution ◽

Dissolution Behavior

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Long-Acting Risperidone Dual Control System: Preparation, Characterization and Evaluation In Vitro and In Vivo

Pharmaceutics ◽

10.3390/pharmaceutics13081210 ◽

2021 ◽

Vol 13 (8) ◽

pp. 1210

Author(s):

Xieguo Yan ◽

Shiqiang Wang ◽

Kaoxiang Sun

Keyword(s):

Drug Loading ◽

Entrapment Efficiency ◽

In Vitro Dissolution ◽

Dissolution Behavior ◽

In Vivo Evaluation ◽

Long Term Treatment ◽

Long Acting

Schizophrenia, a psychiatric disorder, requires long-term treatment; however, large fluctuations in blood drug concentration increase the risk of adverse reactions. We prepared a long-term risperidone (RIS) implantation system that can stabilize RIS release and established in-vitro and in-vivo evaluation systems. Cumulative release, drug loading, and entrapment efficiency were used as evaluation indicators to evaluate the effects of different pore formers, polymer ratios, porogen concentrations, and oil–water ratios on a RIS implant (RIS-IM). We also built a mathematical model to identify the optimized formulation by stepwise regression. We also assessed the crystalline changes, residual solvents, solubility and stability after sterilization, in-vivo polymer degradation, pharmacokinetics, and tissue inflammation in the case of the optimized formulation. The surface of the optimized RIS microspheres was small and hollow with 134.4 ± 3.5 µm particle size, 1.60 SPAN, 46.7% ± 2.3% implant drug loading, and 93.4% entrapment efficiency. The in-vitro dissolution behavior of RIS-IM had zero-order kinetics and stable blood concentration; no lag time was released for over three months. Furthermore, the RIS-IM was not only non-irritating to tissues but also had good biocompatibility and product stability. Long-acting RIS-IMs with microspheres and film coatings can provide a new avenue for treating schizophrenia.

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Antibiotic loaded β-tricalcium phosphate/calcium sulfate for antimicrobial potency, prevention and killing efficacy of Pseudomonas aeruginosa and Staphylococcus aureus biofilms

Scientific Reports ◽

10.1038/s41598-020-80764-6 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Nan Jiang ◽

Devendra H. Dusane ◽

Jacob R. Brooks ◽

Craig P. Delury ◽

Sean S. Aiken ◽

...

Keyword(s):

Tricalcium Phosphate ◽

Calcium Sulfate ◽

Bone Graft Substitute ◽

In Vitro Assays ◽

In Vivo Experiments ◽

Orthopaedic Infections ◽

Clinical Investigations ◽

Antimicrobial Potency

AbstractThis study investigated the efficacy of a biphasic synthetic β-tricalcium phosphate/calcium sulfate (β-TCP/CS) bone graft substitute for compatibility with vancomycin (V) in combination with tobramycin (T) or gentamicin (G) evidenced by the duration of potency and the prevention and killing efficacies of P. aeruginosa (PAO1) and S. aureus (SAP231) biofilms in in vitro assays. Antibiotic loaded β-TCP/CS beads were compared with antibiotic loaded beads formed from a well characterized synthetic calcium sulfate (CS) bone void filler. β-TCP/CS antibiotic loaded showed antimicrobial potency against PAO1 in a repeated Kirby-Bauer like zone of inhibition assay for 6 days compared to 8 days for CS. However, both bead types showed potency against SAP231 for 40 days. Both formulations loaded with V + T completely prevented biofilm formation (CFU below detection limits) for the 3 days of the experiment with daily fresh inoculum challenges (P < 0.001). In addition, both antibiotic loaded materials and antibiotic combinations significantly reduced the bioburden of pre-grown biofilms by between 3 and 5 logs (P < 0.001) with V + G performing slightly better against PAO1 than V + T. Our data, combined with previous data on osteogenesis suggest that antibiotic loaded β-TCP/CS may have potential to stimulate osteogenesis through acting as a scaffold as well as simultaneously protecting against biofilm infection. Future in vivo experiments and clinical investigations are warranted to more comprehensively evaluate the use of β-TCP/CS in the management of orthopaedic infections.

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Effects of Mn-doping on the structure and in vitro degradation of β-tricalcium phosphate

Ceramics International ◽

10.1016/j.ceramint.2021.05.013 ◽

2021 ◽

Author(s):

Junjian Che ◽

Hubiao Wang ◽

Yunhai Ma ◽

Feipeng Cao ◽

Guoqin Liu ◽

...

Keyword(s):

Tricalcium Phosphate ◽

In Vitro Degradation ◽

Mn Doping

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Fabrication, Characterization and In Vitro Cytotoxicity of Mesoporous β-Tricalcium Phosphate Using the Spray Drying Method

Crystals ◽

10.3390/cryst11030252 ◽

2021 ◽

Vol 11 (3) ◽

pp. 252

Author(s):

Henni Setia Ningsih ◽

Leonhard Tannesia ◽

Hsiang-Ho Chen ◽

Shao-Ju Shih

Keyword(s):

Spray Drying ◽

Tricalcium Phosphate ◽

Low Cost ◽

In Vitro Cytotoxicity ◽

Formation Mechanisms ◽

Synthesis Methods ◽

Average Pore ◽

Calcination Temperatures ◽

Future Demands

Mesoporous beta tricalcium phosphate (β-TCP) has recently attracted significant interest as an artificial bone tissue in orthopedics. However, a scalable process is required to meet future demands. Spray drying is one of the potential synthesis methods owing to its low cost and scalable production. In this study, various mesoporous β-TCP powders were calcined in the range of 800 to 1100 °C, with particle sizes ranging from ~0.3 to ~1.8 μm, specific surface areas from ~16 to ~64 m2/g, and average pore sizes of 3 nm. Except for the 800 °C calcined powder, the other β-TCP powders (calcination temperatures of 900, 1000, and 1100 °C) exhibited no cytotoxicity. These results indicate that spray-dried mesoporous β-TCP powders were obtained. Finally, the corresponding formation mechanisms are discussed.

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Effect of Gold Nanoparticles and Silicon on the Bioactivity and Antibacterial Properties of Hydroxyapatite/Chitosan/Tricalcium Phosphate-Based Biomicroconcretes

Materials ◽

10.3390/ma14143854 ◽

2021 ◽

Vol 14 (14) ◽

pp. 3854

Author(s):

Joanna Czechowska ◽

Ewelina Cichoń ◽

Anna Belcarz ◽

Anna Ślósarczyk ◽

Aneta Zima

Keyword(s):

Gold Nanoparticles ◽

Antibacterial Activity ◽

Tricalcium Phosphate ◽

Setting Time ◽

Antibacterial Properties ◽

Bone Substitutes ◽

Bacterial Strains ◽

Setting Times ◽

Biological Studies

Bioactive, chemically bonded bone substitutes with antibacterial properties are highly recommended for medical applications. In this study, biomicroconcretes, composed of silicon modified (Si-αTCP) or non-modified α-tricalcium phosphate (αTCP), as well as hybrid hydroxyapatite/chitosan granules non-modified and modified with gold nanoparticles (AuNPs), were designed. The developed biomicroconcretes were supposed to combine the dual functions of antibacterial activity and bone defect repair. The chemical and phase composition, microstructure, setting times, mechanical strength, and in vitro bioactive potential of the composites were examined. Furthermore, on the basis of the American Association of Textile Chemists and Colorists test (AATCC 100), adapted for chemically bonded materials, the antibacterial activity of the biomicroconcretes against S. epidermidis, E. coli, and S. aureus was evaluated. All biomicroconcretes were surgically handy and revealed good adhesion between the hybrid granules and calcium phosphate-based matrix. Furthermore, they possessed acceptable setting times and mechanical properties. It has been stated that materials containing AuNPs set faster and possess a slightly higher compressive strength (3.4 ± 0.7 MPa). The modification of αTCP with silicon led to a favorable decrease of the final setting time to 10 min. Furthermore, it has been shown that materials modified with AuNPs and silicon possessed an enhanced bioactivity. The antibacterial properties of all of the developed biomicroconcretes against the tested bacterial strains due to the presence of both chitosan and Au were confirmed. The material modified simultaneously with AuNPs and silicon seems to be the most promising candidate for further biological studies.

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