Fabrication of Nano-Fibrous Poly(L-Lactic Acid) Scaffold Enhanced by Silane Modified Chitosan Fibers

2012 ◽  
Vol 152-154 ◽  
pp. 609-612
Author(s):  
Xue Jun Wang ◽  
Tao Lou ◽  
Xiu Ting Lang ◽  
Guo Jun Song
Keyword(s):  
Composite Scaffold ◽  
Fiber Composite ◽  
Fiber Surface ◽  
High Porosity ◽  
Fibrous Scaffold ◽  
Nano Composite ◽  
Modified Chitosan ◽  
Fiber Size ◽  
Chitosan Fiber ◽  
Surface Modified

In this study, A PLLA/silane modified chitosan fiber composite scaffold was sucessfully prepared using thermal induced phase separation method. The PLLA was chemically coupled with chitosan fiber surface using γ-methacryloxypropyl-trimethoxysilane.The composite scaffold had a nano-fibrous PLLA matrix (fiber size 200-750 nm), an interconnective pores ((1-6 μm), high porosity (>90%). Introduced surface modified chitosan fibers into PLLA matrix, it significantly enhanced the nano-fibrous scaffold. The new nano composite scaffold is potentially a very promising scaffold for tissue engineering.

Preparation of Nano-Fibrous Poly(L-lactic acid) Scaffold with Hierarchical Pores

2011 ◽  
Vol 418-420 ◽  
pp. 303-306
Author(s):  
Xue Jun Wang ◽  
Tao Lou ◽  
Guo Jun Song
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Pore Size ◽  
Composite Scaffold ◽  
Compressive Modulus ◽  
High Porosity ◽  
Nano Composite ◽  
Hierarchical Pores ◽  
Fiber Size ◽  
Hierarchical Pore

In this study, a nano-fibrous PLLA scaffold with hierarchical pore was sucessfully fabricated using combined TIPS and particle leaching method.The scaffold had a nano-fibrous PLLA matrix (fiber size 100-800 nm), an interconnective hierarchical pores (1.0- 425 μm), high porosity (>96%). The compressive modulus of scaffold with different pore size was between 0.16 MPa to 0.2 Mpa and it decreased with the increased salt size embedded in. The new nano composite scaffold is potentially a very promising scaffold for tissue engineering.

Carboxymethyl Chitosan/Hydroxyapatite Composite Scaffold for Bone Regeneration

2010 ◽  
Vol 123-125 ◽  
pp. 299-302 ◽  
Author(s):  
Xu Xu Bao ◽  
Yuan Li ◽  
Akira Teramoto ◽  
Koji Abe
Keyword(s):  
Bone Regeneration ◽  
Composite Scaffold ◽  
Carboxymethyl Chitosan ◽  
Fibrous Scaffold ◽  
Modified Chitosan ◽  
Micro Particles ◽  
Proliferation And Differentiation ◽  
Chitosan Scaffolds ◽  
Ft Ir ◽  
Surface Morphologies

Natural bone is a composite mainly made from nano/micro-structure of hydroxyapatite and collagen fibers. For bone regeneration by tissue engineering, it is important to synthesize nano-composites with good biocompatibility, high bioactivity and great bonding property as potentially useful scaffold. In this study, we fabricated chitosan nano-nonwoven scaffold via electrospinning and modified chitosan scaffolds by carboxymethylation (CM). Moreover scaffolds were macerated in SBF (simulated body fluid) to form hydroxyapatite on its surface. Surface morphologies (SEM) showed that nano/micro particles formed on the surface of the carboxymethyl chitosan fibrous scaffold. Results of FT-IR and XRD confirmed that the nano/micro particles were hydroxyapatite crystalline. Moreover by employed mice osteoblast (MC3T3-E1) cell for adhesion, proliferation and differentiation assays, and the hydroxyapatite particles appeared to have a great effect on the late stages of osteoblast behavior (alkaline phosphatase ).

Preparation and properties of biodegradable polymer/nano-hydroxyapatite bioceramic scaffold for spongy bone regeneration

2019 ◽  
Vol 39 (2) ◽  
pp. 134-142 ◽  
Author(s):  
Mohammed Mizanur Rahman ◽  
Md. Shahruzzaman ◽  
Md. Sazedul Islam ◽  
M. Nuruzzaman Khan ◽  
Papia Haque
Keyword(s):  
Compressive Strength ◽  
Biodegradable Polymer ◽  
Composite Scaffold ◽  
Sol Gel ◽  
Nanocrystalline Structure ◽  
Poly Lactic Acid ◽  
Cytotoxicity Test ◽  
High Porosity ◽  
Spongy Bone ◽  
Excellent Thermal Stability

Abstract Biodegradable polymer/bioceramic composite scaffolds can overcome the limitations of conventional ceramic bone substitutes, such as brittleness and difficulty in shaping. To better mimic the mineral components and microstructure of natural bone, a novel nano-hydroxyapatite (nHAp)–chitosan composite scaffold including gelatin and polymer (poly(lactic acid)) with high porosity was developed using a sol-gel method and subsequently lyophilized for efficient bone tissue engineering. The nanocrystalline structure of hydroxyapatite was observed using X-ray diffraction analysis and the composite showed crystallinity due to the presence of nHAp. The pore diameter of the composite containing 5% nHAp was found to be 125 μm, while the composites with 10%, 15%, and 20% nHAp revealed a smaller pore size in the range of 15–28 μm. The highest compressive strength of 5.5 MPa was observed for the 10% nHAp-containing scaffold, whereas thermogravimetric analysis showed 90%–94% degradation at a temperature of 600°C, which demonstrated its excellent thermal stability. Antibacterial and cytotoxicity test results revealed that the composite is resistant toward microbial attack and has low sensitivity in cytotoxicity. The compressive strength data suggests that the composite does not have enough strength as that of human compact bone; however, the highly porous structure as observed in scanning electron microscopy makes it possible for use as an excellent substrate in the spongy bone of humans.

scholarly journals In-situ Preparation of Nano-calcium Carbonate/Cellulose Fiber Composite and Its Application in Fluff Pulp

2017 ◽  
Vol 12 (3) ◽  
pp. 155892501701200 ◽  
Author(s):  
Yongjian Xu ◽  
Chunmei Jiang ◽  
Chao Duan ◽  
Weipeng Zhang
Keyword(s):  
Hydrogen Bonding ◽  
Calcium Carbonate ◽  
Adsorption Capacity ◽  
Fiber Composite ◽  
Fiber Surface ◽  
Cellulose Fiber ◽  
Natural Polymers ◽  
Inorganic Particles ◽  
In Situ Preparation

Calcium carbonate/cellulose fiber composites combining natural polymers and inorganic particles are promising materials for preparing fluff pulp. In this study, calcium carbonate (CaCO3) particles were in-situ precipitated on cellulose fiber surfaces to decrease the level of hydrogen bonding. The results showed that nano CaCO3 particles precipitated and dispersed well on the fiber surface. The fluff pulp made from cellulose/CaCO3 modified fibers exhibited high effectiveness in the dry defibration process and good adsorption capacity due to the weak hydrogen bonding. The burst index of the handsheets decreased 31% (based on the ash content of 1.48 wt. %) without compromising the adsorption capacity of the fluff pulp.

scholarly journals A biodegradable porous composite scaffold of PCL/BCP containing Ang-(1-7) for bone tissue engineering

Cerâmica ◽  
2012 ◽  
Vol 58 (348) ◽  
pp. 481-488 ◽  
Author(s):  
F. A. Macedo ◽  
E. H. M. Nunes ◽  
W. L. Vasconcelos ◽  
R. A. Santos ◽  
R. D. Sinisterra ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Composite Scaffold ◽  
Weight Ratio ◽  
High Porosity ◽  
X Rays ◽  
Solvent Casting

Highly porous three-dimensional biodegradable scaffolds was obtained from beta-tricalcium phosphate-hydroxyapatite bioceramic (BCP), PCL, and Angiotensin-(1-7). We used the solvent casting and particulate leaching methods (SC/PL). The processed scaffolds were characterized by X-ray microtomography (µ-CT). Biocompatibility tests in vitro were performed during three and seven days using MTT and Alkaline Phosphatase Activity (APA) assays. Both the MTT activity and APA were evaluated using a one-way ANOVA test. The µ-CT results showed that the increase of the PCL:BCP weight ratio leads to structures with lower pore sizes. The pore interconnectivity of the processed scaffolds was evaluated in terms of the fragmentation index (FI). We observed that the obtained composites present poorly connected structures, with close values of FI. However, as the polymer phase is almost transparent to the X-rays, it was not taken into consideration in the µ-CT tests. The MTT activity assay revealed that scaffolds obtained with and without Angiotensin-(1-7) present mild and moderate cytotoxic effects, respectively. The APA assay showed that the rat osteoblasts, when in contact for three days with the PCL composites, presented an APA similar to that observed for the control cells. Nevertheless, for an incubation time of seven days we observed a remarkable decrease in the alkaline phosphatase activity. In conclusion, using the solvent casting and salt leaching method we obtained 3D porous that are composites of PCL, BC and Ang-(1-7), which have suitable shapes for the bone defects, a high porosity and interconnect pores. Furthermore, the viability in vitro showed that the scaffolds have potential for drug delivery system and could be used in future in vivo tests.

XPS study of PBO fiber surface modified by incorporation of hydroxyl polar groups in main chains

2010 ◽  
Vol 256 (7) ◽  
pp. 2073-2075 ◽  
Author(s):  
Tao Zhang ◽  
Dayong Hu ◽  
Junhong Jin ◽  
Shenglin Yang ◽  
Guang Li ◽  
...  
Keyword(s):  
Fiber Surface ◽  
Polar Groups ◽  
Pbo Fiber ◽  
Surface Modified ◽  
Xps Study

Nanofibrous Scaffold Engineering Using Electrospinning

2007 ◽  
Vol 7 (12) ◽  
pp. 4595-4603 ◽  
Author(s):  
R. Murugan ◽  
Z. M. Huang ◽  
F. Yang ◽  
S. Ramakrishna
Keyword(s):  
Tissue Engineering ◽  
Cell Attachment ◽  
Dimensional Space ◽  
High Surface ◽  
Critical Role ◽  
High Surface Area ◽  
Structural Features ◽  
Tissue Growth ◽  
High Porosity ◽  
Fibrous Scaffold

Scaffold plays a critical role in tissue engineering where it provides necessary structural support for the cells to accommodate and to guide their growth in the three dimensional space into a specific tissue. Therefore, engineering scaffolds favorable for cell/tissue growth is of great importance and a pre-requisite for scaffold-based tissue engineering. Electrospinning is a versatile method that has been recently adapted in engineering nano-fibrous scaffolds that mimic the structural features of biological extracellular matrix (ECM). It offers many advantages over conventional scaffold methodologies, for example, capable of producing ultra-fine fibers with high porosity, high spatial orientation, high aspect ratio, and high surface area, which are highly required for the initial cell attachment, tissue formation, and continued function. Considering these astonishing merits, this article emphasis on nano-fibrous scaffold engineering by electrospinning.

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