Preparation and Characterization of Layer Structured Porous Chitosan Scaffold for Tissue Engineering

2012 ◽  
Vol 198-199 ◽  
pp. 179-182
Author(s):  
Guo Jun Song ◽  
Xue Jun Wang ◽  
Tao Lou ◽  
Li Yong Lv
Keyword(s):  
Tissue Engineering ◽  
Acetic Acid ◽  
Phase Separation ◽  
Layer Structure ◽  
Compressive Modulus ◽  
High Porosity ◽  
Chitosan Scaffold ◽  
Chitosan Concentration ◽  
Porous Chitosan

In this study, layer structured porous chitosan scaffold was successfully fabricated using thermal induced phase separation method. The scaffold had a layer structure with interconnective pores (50- 300 μm) and high porosity (>90%) using citric or acetic acid as the solvent. However, the results of compressive modulus of the scaffold showed that acetic acid was a better choice, and the compressive modulus of scaffold increased with chitosan concentration in acetic acid. The scaffold is very promising for tissue engineering.

Preparation of Nano-Fibrous Poly(L-lactic acid) Scaffold with Hierarchical Pores

2011 ◽  
Vol 418-420 ◽  
pp. 303-306
Author(s):  
Xue Jun Wang ◽  
Tao Lou ◽  
Guo Jun Song
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Pore Size ◽  
Composite Scaffold ◽  
Compressive Modulus ◽  
High Porosity ◽  
Nano Composite ◽  
Hierarchical Pores ◽  
Fiber Size ◽  
Hierarchical Pore

In this study, a nano-fibrous PLLA scaffold with hierarchical pore was sucessfully fabricated using combined TIPS and particle leaching method.The scaffold had a nano-fibrous PLLA matrix (fiber size 100-800 nm), an interconnective hierarchical pores (1.0- 425 μm), high porosity (>96%). The compressive modulus of scaffold with different pore size was between 0.16 MPa to 0.2 Mpa and it decreased with the increased salt size embedded in. The new nano composite scaffold is potentially a very promising scaffold for tissue engineering.

Characterization of novel calcium hydroxide‐mediated highly porous chitosan‐calcium scaffolds for potential application in dentin tissue engineering

2020 ◽  
Vol 108 (6) ◽  
pp. 2546-2559 ◽  
Author(s):  
Diana Gabriela Soares ◽  
Ester Alves Ferreira Bordini ◽  
Fernanda Balestrero Cassiano ◽  
Erika Soares Bronze‐Uhle ◽  
Leandro Edgar Pacheco ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Calcium Hydroxide ◽  
Potential Application ◽  
Porous Chitosan ◽  
Highly Porous

scholarly journals Synthesis and Characterization of Biocompatible Methacrylated Kefiran Hydrogels: Towards Tissue Engineering Applications

Polymers ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1342
Author(s):  
Hajer Radhouani ◽  
Susana Correia ◽  
Cristiana Gonçalves ◽  
Rui L. Reis ◽  
Joaquim M. Oliveira
Keyword(s):  
Tissue Engineering ◽  
Biological Properties ◽  
Synthesis And Characterization ◽  
Extrusion Force ◽  
High Porosity ◽  
Micro Ct ◽  
Pseudoplastic Fluid ◽  
Physical And Chemical ◽  
Chemical Cross Linking

Hydrogel application feasibility is still limited mainly due to their low mechanical strength and fragile nature. Therefore, several physical and chemical cross-linking modifications are being used to improve their properties. In this research, methacrylated Kefiran was synthesized by reacting Kefiran with methacrylic anhydride (MA). The developed MA-Kefiran was physicochemically characterized, and its biological properties evaluated by different techniques. Chemical modification of MA-Kefiran was confirmed by 1H-NMR and FTIR and GPC-SEC showed an average Mw of 793 kDa (PDI 1.3). The mechanical data obtained revealed MA-Kefiran to be a pseudoplastic fluid with an extrusion force of 11.21 ± 2.87 N. Moreover, MA-Kefiran 3D cryogels were successfully developed and fully characterized. Through micro-CT and SEM, the scaffolds revealed high porosity (85.53 ± 0.15%) and pore size (33.67 ± 3.13 μm), thick pore walls (11.92 ± 0.44 μm) and a homogeneous structure. Finally, MA-Kefiran revealed to be biocompatible by presenting no hemolytic activity and an improved cellular function of L929 cells observed through the AlamarBlue® assay. By incorporating methacrylate groups in the Kefiran polysaccharide chain, a MA-Kefiran product was developed with remarkable physical, mechanical, and biological properties, resulting in a promising hydrogel to be used in tissue engineering and regenerative medicine applications.

scholarly journals Preparation and Characterization of Porous Scaffolds Based on Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)

Life ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 935
Author(s):  
Asiyah Esmail ◽  
João R. Pereira ◽  
Chantal Sevrin ◽  
Christian Grandfils ◽  
Ugur Deneb Menda ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Porous Scaffolds ◽  
High Porosity ◽  
Solvent Casting ◽  
Elongation At Break ◽  
Particulate Leaching ◽  
Copolymer Poly ◽  
Water Swelling ◽  
Emulsion Templating

Poly(hydroxyalkanoates) (PHAs) with different material properties, namely, the homopolymer poly(3-hydroxybutyrate), P(3HB), and the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate, P(3HB-co-3HV), with a 3HV of 25 wt.%, were used for the preparation of porous biopolymeric scaffolds. Solvent casting with particulate leaching (SCPL) and emulsion templating were evaluated to process these biopolymers in porous scaffolds. SCPL scaffolds were highly hydrophilic (>170% swelling in water) but fragile, probably due to the increase of the polymer’s polydispersity index and its high porosity (>50%). In contrast, the emulsion templating technique resulted in scaffolds with a good compromise between porosity (27–49% porosity) and hydrophilicity (>30% water swelling) and without impairing their mechanical properties (3.18–3.35 MPa tensile strength and 0.07–0.11 MPa Young’s Modulus). These specifications are in the same range compared to other polymer-based scaffolds developed for tissue engineering. P(3HB-co-3HV) displayed the best overall properties, namely, lower crystallinity (11.3%) and higher flexibility (14.8% elongation at break. Our findings highlight the potency of our natural biopolyesters for the future development of novel porous scaffolds in tissue engineering, thanks also to their safety and biodegradability.

Preparation and Properties of Biodegradable Polyurethane Scaffolds for Tissue Engineering

2014 ◽  
Vol 513-517 ◽  
pp. 95-99
Author(s):  
Xian Yun He ◽  
Yong Jun Xu ◽  
Ying Jun Wang ◽  
Gang Wu ◽  
Bo Yuan
Keyword(s):  
Tissue Engineering ◽  
Phase Separation ◽  
Porous Scaffolds ◽  
High Porosity ◽  
Inverse Technique ◽  
Compression Property ◽  
Salt Leaching ◽  
Biodegradable Polyurethane

3D porous scaffolds were produced by a combined salt leaching-phase inverse technique. Results showed that micorpores and macropores distributed in the produced scaffolds with high porosity (more than 75%). The interconnectivity can be influenced by the use of methanol and water as a nonsolvent to induce liquidliquid phase separation. The addition of the nonsolvent and NaCl had influence on compression property of the scaffolds.

Lactose Modified Chitosan Scaffold for Hepatocytes Culture

2007 ◽  
Vol 353-358 ◽  
pp. 2147-2150 ◽  
Author(s):  
Ji Lun Pan ◽  
Zhi Ming Bao ◽  
Wei Chen ◽  
Li Li ◽  
Jie Liang Li ◽  
...  
Keyword(s):  
Cell Attachment ◽  
Porous Scaffold ◽  
Porous Scaffolds ◽  
High Porosity ◽  
Urea Synthesis ◽  
Albumin Secretion ◽  
Liver Support ◽  
Chitosan Scaffold ◽  
Bioartificial Liver Support ◽  
Porous Chitosan

The chitosan scaffold we prepared have a high porosity of about 90% with pore sizes from 50 to 200m. Lactose was conjugated onto the inner surface of the highly porous chitosan scaffold. It was used as substrate for rat hepatocytes culture. The cell attachment ratio was much higher than on monolayer membrane and non-modified porous scaffold. Metabolic activities of the cells were evaluated in terms of albumin secretion and urea synthesis. It was found that hepatocytes cultured on the modified scaffolds showed an increase in albumin secretion during the first 4 days and were more stable than that on non-modified scaffold. The results showed that the microstructure of porous scaffolds provides large surface for cells to adhere and facilitates nutrient and oxygen transportation. Such lactose modified scaffold has a potential application in bioartificial liver support system.

Micro and Nano Design and Fabrication of a Novel Artificial Photosynthesis Device

2012 ◽  
Author(s):  
Xiang Ren ◽  
Qingwei Zhang ◽  
Ho-lung Li ◽  
Jack Zhou
Keyword(s):  
Acetic Acid ◽  
Rapid Prototyping ◽  
Artificial Photosynthesis ◽  
3D Scaffold ◽  
Light Reaction ◽  
Chitosan Scaffold ◽  
Reaction Solution ◽  
Micro Channels ◽  
Porous Chitosan ◽  
Proper Material

Artificial photosynthesis is a new method to generate sustainable energy. In order to constrain reaction solution in a solid state structure and increase the reaction efficiency, we designed a novel artificial photosynthesis device with porous chitosan scaffold with interconnected micro-channels. We built 3D interconnected chitosan channels with a home-made heterogeneous 3D rapid prototyping machine, and we used lyophilization method to generate the nano pores inside the chitosan scaffold. Chitosan gel in acetic acid can form different viscosities by different chitosan’s molecular weight and the different concentrations of both chitosan and the acetic acid, so we found a proper material recipe to construct 3D scaffold by our own rapid prototyping machine. Optional support material sodium bicarbonate is used in printing 3D scaffold for holding the printed structure permanently, and the result shows that this method can make the scaffold stronger and harmless to further processes such as adding light reaction units and dark reaction solution into the device.

Preparation and Characterization of Porous PLGA Scaffold for Tissue Engineering

2014 ◽  
Vol 898 ◽  
pp. 322-325
Author(s):  
Xue Jun Wang ◽  
Tao Lou ◽  
Zhen Yang ◽  
Kun Peng He
Keyword(s):  
Tissue Engineering ◽  
Mixed Solvent ◽  
Solvent System ◽  
High Porosity ◽  
Thermally Induced Phase Separation ◽  
Plga Scaffold ◽  
Thermally Induced ◽  
Fibrous Matrix ◽  
Solvent Systems

Scaffold plays an important role in tissue engineering. In this study, porous PLGA scaffold was successfully prepared by mixed solvent systems using the thermally induced phase separation method. The PLGA scaffold shows fibrous matrix and interconnective pores, and the scaffold has high porosity and compressive modulus with dioxane/THF solvent system, which could be a very promising scaffold for tissue engineering.

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