Dynamics Analysis of Positioning Platform in LED Packaging Equipment

2014 ◽  
Vol 494-495 ◽  
pp. 743-746
Author(s):  
Yong Shan Xiao ◽  
Jun Xia Jiang ◽  
Zhen Yu Zhao
Keyword(s):  
Theoretical Basis ◽  
Dynamics Simulation ◽  
Dynamics Analysis ◽  
Assembly Process ◽  
Machine Performance ◽  
Operation Speed ◽  
Practical Guidance ◽  
The Stability ◽  
Assembly Precision ◽  
Speed And Accuracy

With the continuous development of LED industry, speed and accuracy requirements of LED packaging equipment is more and more high. To further improve the operation speed, it is very important to improve the stability of the positioning platform. Based on MSC.ADAMS(Automatic Dynamic Analysis of Mechanical Systems),the dynamics simulation model of the positioning platform in LED packaging equipment is established, and the effects of the assembly precision is researched on the machine performance. The method provides the theoretical basis and practical guidance for LED assembly process, and thereby can improve the operation speed and stability of the packaging equipment.

Functional characterization and structural modelling of peptidoglycan degrading β-N-acetyl-glucosaminidase from a dental isolate of Serratia marcescens

2020 ◽  
Vol 23 ◽  
Author(s):  
Aditi Rathee ◽  
Anil Panwar ◽  
Seema Kumari ◽  
Sanjay Chhibber ◽  
Ashok Kumar
Keyword(s):  
Functional Characterization ◽  
Major Change ◽  
Crystal Form ◽  
Bound State ◽  
Dynamics Simulation ◽  
Gram Positive ◽  
Structural Cell ◽  
Stability Structure ◽  
Arginine Residues ◽  
The Stability

Introduction:: Enzymatic degradation of peptidoglycan, a structural cell wall component of Gram-positive bacteria, has attracted considerable attention being a specific target for many known antibiotics. Methods:: Peptidoglycan hydrolases are involved in bacterial lysis through peptidoglycan degradation. β-N-acetylglucosaminidase, a peptidoglycan hydrolase, acts on O-glycosidic bonds formed by N-acetylglucosamine and N-acetyl muramic acid residues of peptidoglycan. Aim of present study was to study the action of β-N-acetylglucosaminidase, on methicillin- resistant Staphylococcus aureus (MRSA) and other Gram-negative bacteria. Results:: We investigated its dynamic behaviour using molecular dynamics simulation and observed that serine and alanine residues are involved in catalytic reaction in addition to aspartic acid, histidine, lysine and arginine residues. When simulated in its bound state, the RMSD values were found lesser than crystal form in the time stamp of 1000 picoseconds revealing its stability. Structure remained stably folded over 1000 picoseconds without undergoing any major change further confirming the stability of complex. Conclusion:: It can be concluded that enzymes belonging to this category can serve as a tool in eradicating Gram-positive pathogens and associated infections.

scholarly journals Rational thermostabilisation of four-helix bundle dimeric de novo proteins

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shin Irumagawa ◽  
Kaito Kobayashi ◽  
Yutaka Saito ◽  
Takeshi Miyata ◽  
Mitsuo Umetsu ◽  
...  
Keyword(s):  
Protein Design ◽  
De Novo ◽  
Protein Complexes ◽  
Salt Bridge ◽  
Dynamics Simulation ◽  
Saturation Mutagenesis ◽  
Helix Bundle ◽  
Stable Mutant ◽  
Four Helix Bundle ◽  
The Stability

AbstractThe stability of proteins is an important factor for industrial and medical applications. Improving protein stability is one of the main subjects in protein engineering. In a previous study, we improved the stability of a four-helix bundle dimeric de novo protein (WA20) by five mutations. The stabilised mutant (H26L/G28S/N34L/V71L/E78L, SUWA) showed an extremely high denaturation midpoint temperature (Tm). Although SUWA is a remarkably hyperstable protein, in protein design and engineering, it is an attractive challenge to rationally explore more stable mutants. In this study, we predicted stabilising mutations of WA20 by in silico saturation mutagenesis and molecular dynamics simulation, and experimentally confirmed three stabilising mutations of WA20 (N22A, N22E, and H86K). The stability of a double mutant (N22A/H86K, rationally optimised WA20, ROWA) was greatly improved compared with WA20 (ΔTm = 10.6 °C). The model structures suggested that N22A enhances the stability of the α-helices and N22E and H86K contribute to salt-bridge formation for protein stabilisation. These mutations were also added to SUWA and improved its Tm. Remarkably, the most stable mutant of SUWA (N22E/H86K, rationally optimised SUWA, ROSA) showed the highest Tm (129.0 °C). These new thermostable mutants will be useful as a component of protein nanobuilding blocks to construct supramolecular protein complexes.

scholarly journals Treatment of Rheumatoid Arthritis with Traditional Chinese Medicine

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Wen-Yuan Lee ◽  
Hsin-Yi Chen ◽  
Kuan-Chung Chen ◽  
Calvin Yu-Chian Chen
Keyword(s):  
Rheumatoid Arthritis ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Protein Complex ◽  
Chronic Inflammatory Disease ◽  
Dynamics Simulation ◽  
Current Therapy ◽  
Potential Candidate ◽  
Interleukin 6 Receptor ◽  
The Stability

Rheumatoid arthritis (RA) is a chronic inflammatory disease that will affect quality of life and, working efficiency, and produce negative thoughts for patients. Current therapy of RA is treated with disease-modifying antirheumatic drugs (DMARDs). Although most of these treatment methods are effective, most patients still have a pleasant experience either due to poor efficacy or side effects or both. Interleukin-6 receptor (IL6R) is important in the pathogenesis of RA. In this study, we would like to detect the potential candidates which inhibit IL6R against RA from traditional Chinese medicine (TCM). We use TCM compounds from the TCM Database@Taiwan for virtually screening the potential IL6R inhibitors. The TCM candidate compound, calycosin, has potent binding affinity with IL6R protein. The molecular dynamics simulation was employed to validate the stability of interaction in the protein complex with calycosin. The analysis indicates that protein complex with calycosin is more stable. In addition, calycosin is known to be one of the components ofAngelica sinensis, which has been indicated to have an important role in the treatment of rheumatoid arthritis. Therefore, calycosin is a potential candidate as lead compounds for further study in drug development process with IL6R protein against rheumatoid arthritis.

Comprehensive identification of aircraft coordination feature based on complete importance modeling and its engineering application

2018 ◽  
Vol 38 (4) ◽  
pp. 398-411 ◽  
Author(s):  
Feiyan Guo ◽  
Fang Zou ◽  
Jian Hua Liu ◽  
Bo Zhao ◽  
Zhongqi Wang
Keyword(s):  
Engineering Application ◽  
Laboratory Method ◽  
Correction Coefficient ◽  
Assembly Process ◽  
Quality Loss ◽  
Content Type ◽  
Aircraft Manufacturing ◽  
Close Relationship ◽  
Geometric Size ◽  
Assembly Precision

Purpose Coordination feature (CF) is the information carrier in dimension and shape transfer process in aircraft manufacturing. The change of its geometric size, shape, position or other attributes would affect the consistency of accumulated errors between two or more assemblies. To identify these “key characteristics” that have a close relationship with the assembly precision, a comprehensive method was developed under digital manufacturing environment, which was based on importance calculation. The multi-hierarchy and multi-station assembly process of aircraft products were also taken into consideration. Design/methodology/approach First, the interaction and evaluation relationship between components at different manufacturing stages was decomposed with a hierarchical net. Second, to meet coordination accuracy requirements, with the integrated application of Taguchi quality loss function, accuracy principal and error correction coefficient H, the quality loss between target features and candidate features at adjacent assembly hierarchies were calculated, which was based on their precision variation. Third, the influence degree and affected degree of the features were calculated with DEMATEL (decision-making trial and evaluation laboratory) method, and the concepts of centrality degree index and cause degree index were proposed for calculating the complete importance degree to eventually identify the CFs. Findings Based on the proposed methodology, CFs, affecting the skin profile and the flush coordination accuracy, were successfully identified at different assembly hierarchies to a certain type of wing flap component. Originality/value Benefit results for the engineering application showed that the deviation of skin profile was more accurate than before, and the tolerance was also closer to the centerline of required assembly precision range. Moreover, the stability in the assembly process was increased by 26.9 per cent, which could bring a higher assembly quality and an enhancement on aircraft’s flight performance.

A Modal Analysis of Giant Shipbuilding Tower Crane

2012 ◽  
Vol 239-240 ◽  
pp. 473-477 ◽  
Author(s):  
Guo Jian Huang ◽  
Cheng Zhong He ◽  
Xin Hua Wang
Keyword(s):  
Modal Analysis ◽  
Natural Frequency ◽  
Theoretical Basis ◽  
Safety Evaluation ◽  
Structural Instability ◽  
Dynamics Analysis ◽  
Vibration Frequencies ◽  
Corrosive Environment ◽  
Tower Crane ◽  
Modal Vibration

Old giant cranes in corrosive environment are in serious hidden danger of structural instability and failure, the modal analysis is an important part of dynamics analysis in the Crane Safety Evaluation to guarantee the safety of cranes. A Modal Analysis of a 28-year old 100t giant shipbuilding tower crane was done using ANSYS. Comparing with the vibration frequencies and modal shapes, Modal analysis shows that the Natural Frequency of the crane reduces from 1.0024Hz to 1.0003Hz, beside all the Modal vibration frequencies (first 10 Order) of the crane are lower than the designed value, means the strength of the tower crane has decreased; the Modal Vibration Frequencies (first 10 Order) of the tower crane are between 1 Hz and 2 Hz. The Mmodal analysis provided theoretical basis for safe use, design and transformation of this crane.

Virtual Screening of Phyto Chemicals Against SARS-CoV-2 Targets

2021 ◽  
Vol 6 (3) ◽  
pp. 61-76
Author(s):  
Shahanas Naisam ◽  
Vidhya V. S. ◽  
Suvanish Kumar ◽  
Nidhin Sreekumar
Keyword(s):  
Dynamics Simulation ◽  
Spike Protein ◽  
Ligand Interaction ◽  
Docking Analysis ◽  
Drug Molecules ◽  
In Vivo Analysis ◽  
Protein Ligand Interaction ◽  
The Stability ◽  
3Cl Protease

The COVID-19 pandemic wave has recommenced and is spreading like wildfire across the globe. The well-reported antiviral potency of phyto compounds could offer potential drug molecules for the current predicament. The present study analyses the molecular interaction of selected phyto compounds and SARS-CoV-2 molecular target proteins, namely spike protein, RNA-dependent RNA polymerase, 3C-like proteases, and papain-like protease. Ten newly modeled ligands were also considered for the study. Molecular docking analysis was carried out independently using MOE, AutoDock Vina, Schrodinger-Glide, and the stability of protein-ligand interaction was validated through molecular dynamics simulation. Petunidin interacts with spike protein resulting in a good Gscore, binding energy, and H-bond interaction. Also, alions, letestuianin-A, (+)-pinitol show better interaction with RdRp, 3CL-protease, and papain-like protease, respectively. The presented work screens through 2314 ligands to yield top-ranked molecules which could be taken up to develop potential lead molecules via in-vivo analysis.

Reframing Information System Design as Learning Across Communities of Practice

2009 ◽  
pp. 2052-2070
Author(s):  
Kevin Gallagher ◽  
Robert M. Mason
Keyword(s):  
Knowledge Transfer ◽  
Communities Of Practice ◽  
Theoretical Basis ◽  
Systems Design ◽  
Past Research ◽  
Three Dimensions ◽  
Information System Design ◽  
Requirements Definition ◽  
Practical Guidance ◽  
Definition Phase

This article frames the requirements definition phase of systems design as a problem of knowledge transfer and learning between two communities of practice: IS designers and system users. The theoretical basis for the proposed approach is Wenger’s (1998) framework for social learning, which involves three dimensions: alignment, imagination, and engagement. The article treats the requirements definition task in systems design as a set of activities involving mutual learning and knowledge transfer between two communities of practice (CoP) along these three dimensions. In taking this approach, the article maps the results of past research on the systems design process onto this CoP framework and illustrates that the proposed framework encompasses the same activities used by traditional methods of requirements definition. However, this approach focuses attention on the learning that must take place between the two CoPs and thereby helps resolve some of the inherent shortcomings of prior efforts and approaches. The framework provides both a more encompassing conceptual lens for research on improving the requirements definition task and practical guidance for managers who are charged with a systems design project.

Sign in / Sign up

Export Citation Format

Share Document