Low Light Affacting Alkaloids Accumulation and Related Biosynthetic Pathway Genes Expression in Leaves of Catharanthus roseus Seedlings

2014 ◽  
Vol 522-524 ◽  
pp. 311-315
Author(s):  
Ying Liu ◽  
De Wen Li ◽  
Zhong Hua Zhang ◽  
Yuan Gang Zu
Keyword(s):  
Gene Expression ◽  
Catharanthus Roseus ◽  
High Performance ◽  
Light Condition ◽  
Content Increase ◽  
Biosynthesis Pathway ◽  
Expression Change ◽  
Low Light ◽  
Plastic Films ◽  
Genes Expression

The alkaloids contents were investigated by High performance liquid chromatography (HPLC) in Catharanthus roseus aseptic seedlings leaves, the expressions of terpenoid indole alkaloids (TIAs) biosynthesis pathway genes were detected by semi-quantitative RT-PCR. The plastic films treatment induces a low light condition. HPLC analysis shows, the contents of vindoline (VIN) and catharanthine (CAT) are slight increase first and significant decrease (p<0.05) under plastic films, the vinblastine (VBL) content increase and reach the maximum (0.028 mg g-1±0.0051) on the 15th day of treatment. TIA biosynthesis pathway genes expression change under low light. The correlation analysis indicates, VIN accumulation is significantly correlated with the gene expression of desacetoxyvindoline-4-hydroxylase (D4h) and deacetylvindoline-4-O-acetyl transferase (Dat), and CAT accumulation is significantly correlated with strictosidine synthase (Str) gene expression (p<0.05). The low light treatment with plastic films accelerates the accumulation of VBL in C. roseus seedlings leaves.

scholarly journals Changing in the production of anticancer drugs (vinblastine and vincristine) in Catharanthus roseus (L.) G. Don by potassium and ascorbic acid treatments  

2022 ◽  
Author(s):  
Neda Sahi ◽  
Akbar Mostajeran ◽  
Mustafa Ghanadian
Keyword(s):  
Gene Expression ◽  
Ascorbic Acid ◽  
Anticancer Drugs ◽  
Catharanthus Roseus ◽  
Peroxidase Activity ◽  
Foliar Application ◽  
Control Plant ◽  
Microtubule Assembly ◽  
Positive Interaction ◽  
Genes Expression

Catharanthus roseus seedling was treated with different concentrations (1.5, 3.16, 15, and 30 mmol) and forms (K<sub>2</sub>SO<sub>4</sub> and KNO<sub>3</sub>) of potassium (K<sup>+</sup>) via Hoagland’s nutrient solution. Ascorbic acid (AsA) was sprayed twice (plant days 68 and 78) with different concentrations (750 and 1 500 mg/L) on the leaves. Vinblastine, vincristine, tryptophan contents, D4H and DAT genes expression, peroxidase activity, and H<sub>2</sub>O<sub>2</sub> content of leaves were measured. Potassium in KNO<sub>3</sub> form increased vinblastine (60%) and vincristine (50%), compared to 30% and 20% using K<sub>2</sub>SO<sub>4</sub>. Vinblastine and vincristine inhibit microtubule assembly and ultimately metaphase-arrested caused by the polymerisation. The genes expression was higher 3 times in KNO<sub>3</sub> and 2.5 times in K<sub>2</sub>SO<sub>4</sub> in excess of K<sup>+</sup>. Foliar application of 750 mg/L AsA led to an increase in vinblastine (20%) and vincristine (16%). Both concentrations of AsA had the same additional effect on the expression of D4H and DAT about 30% and 60%, respectively, compared to the control plant. Tryptophan decreased 2.5 times in excess of K<sup>+</sup> and 35% due to the exterior of AsA. H<sub>2</sub>O<sub>2</sub> decreased while peroxidase activity increased along with AsA treatment. A positive interaction existed between the K<sup>+</sup> and AsA on the amount of vinblastine, vincristine, tryptophan, and gene expression.  

scholarly journals High-performance chemical- and light-inducible recombinases in mammalian cells and mice

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Benjamin H. Weinberg ◽  
Jang Hwan Cho ◽  
Yash Agarwal ◽  
N. T. Hang Pham ◽  
Leidy D. Caraballo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammalian Cells ◽  
High Performance ◽  
Genome Engineering ◽  
Genetic Circuits ◽  
Control Of Gene Expression ◽  
Expression Control ◽  
Gene Expression Control ◽  
High Performance Systems ◽  
Spatiotemporal Control

Abstract Site-specific DNA recombinases are important genome engineering tools. Chemical- and light-inducible recombinases, in particular, enable spatiotemporal control of gene expression. However, inducible recombinases are scarce due to the challenge of engineering high performance systems, thus constraining the sophistication of genetic circuits and animal models that can be created. Here we present a library of >20 orthogonal inducible split recombinases that can be activated by small molecules, light and temperature in mammalian cells and mice. Furthermore, we engineer inducible split Cre systems with better performance than existing systems. Using our orthogonal inducible recombinases, we create a genetic switchboard that can independently regulate the expression of 3 different cytokines in the same cell, a tripartite inducible Flp, and a 4-input AND gate. We quantitatively characterize the inducible recombinases for benchmarking their performances, including computation of distinguishability of outputs. This library expands capabilities for multiplexed mammalian gene expression control.

scholarly journals Proteomic analysis of haem-binding protein from Arabidopsis thaliana and Cyanidioschyzon merolae

2020 ◽  
Vol 375 (1801) ◽  
pp. 20190488 ◽  
Author(s):  
Takayuki Shimizu ◽  
Rintaro Yasuda ◽  
Yui Mukai ◽  
Ryo Tanoue ◽  
Tomohiro Shimada ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Proteomic Analysis ◽  
High Performance ◽  
Biochemical Analysis ◽  
Cyanidioschyzon Merolae ◽  
Genes Expression ◽  
Retrograde Signalling ◽  
Coordinated Expression ◽  
Retrograde Signal ◽  
Affinity Beads

Chloroplast biogenesis involves the coordinated expression of the plastid and nuclear genomes, requiring information to be sent from the nucleus to the developing chloroplasts and vice versa. Although it is well known how the nucleus controls chloroplast development, it is still poorly understood how the plastid communicates with the nucleus. Currently, haem is proposed as a plastid-to-nucleus (retrograde) signal that is involved in various physiological regulations, such as photosynthesis-associated nuclear genes expression and cell cycle in plants and algae. However, components that transduce haem-dependent signalling are still unidentified. In this study, by using haem-immobilized high-performance affinity beads, we performed proteomic analysis of haem-binding proteins from Arabidopsis thaliana and Cyanidioschyzon merolae . Most of the identified proteins were non-canonical haemoproteins localized in various organelles. Interestingly, half of the identified proteins were nucleus proteins, some of them have a similar function or localization in either or both organisms. Following biochemical analysis of selective proteins demonstrated haem binding. This study firstly demonstrates that nucleus proteins in plant and algae show haem-binding properties. This article is part of the theme issue ‘Retrograde signalling from endosymbiotic organelles’.

scholarly journals Shade-Inducible Gene Expression Change in <i>Arabidopsis thaliana</i> at Different Temperatures

2016 ◽  
Vol 07 (02) ◽  
pp. 352-423
Author(s):  
ByungHoon B. Kim ◽  
Kaiesa L. Peets ◽  
Jamekia S. Grant ◽  
Joshua S. Hicks ◽  
Dominique C. Zellous ◽  
...  
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Gene Expression Change ◽  
Expression Change ◽  
Inducible Gene Expression ◽  
Different Temperatures ◽  
Inducible Gene

scholarly journals Diabetes Induced Renal Complications by Leukocyte Activation of Nuclear Factor κ-B and its Regulated Genes Expression

2020 ◽  
Author(s):  
Noura Darwish ◽  
Yousif Elnahas ◽  
Fatmah AlQahtany
Keyword(s):  
Gene Expression ◽  
Large Scale ◽  
Structural Changes ◽  
Kidney Injury ◽  
Leukocyte Activation ◽  
Disease Biomarkers ◽  
Genes Expression ◽  
Nuclear Factor Κ B ◽  
Adhesion Process

Type 2 diabetes mellitus (T2D) is a metabolic disorder characterized by inappropriate insulin function. Despite wide progress in genome studies, defects in gene expression for diabetes prognosis still incompletely identified. Prolonged hyperglycemia activates NF-&kappa;B, which is a main player in vascular dysfunctions of diabetes. Activated NF-&kappa;B, triggers expression of various genes that promote inflammation and cell adhesion process. Alteration of pro-inflammatory and profibrotic gene expression contribute to the irreversible functional and structural changes in the kidney resulting in diabetic nephropathy (DN). To identify the effect of some important NF-&kappa;B related genes on mediation of DN progression, we divided our candidate genes on the basis of their function exerted in bloodstream into three categories (Proinflammatory; NF-&kappa;B, IL-1B, IL-6, TNF-&alpha; and VEGF); (Profibrotic; FN, ICAM-1, VCAM-1) and (Proliferative; MAPK-1 and EGF). We analyzed their expression profile in leukocytes of patients and explored their correlation to diabetic kidney injury features. Our data revealed the overexpression of both proinflammatory and profibrotic genes in DN group when compared to T2D group and were associated positively with each other in DN group indicating their possible role in DN progression. In DN patients, increased expression of proinflammatory genes correlated positively with glycemic control and inflammatory markers indicating their role in DN progression. Our data revealed that the persistent activation NF-&kappa;B and its related genes observed in hyperglycemia might contribute to DN progression and might be a good diagnostic and therapeutic target for DN progression. Large-scale studies are needed to evaluate the potential of these molecules to serve as disease biomarkers.

scholarly journals Diabetes mellitus increased integrins gene expression in rat endometrium at the time of embryo implantation

2019 ◽  
Author(s):  
Abbas Bakhteyari ◽  
Yasaman Zarrin ◽  
Parvaneh Nikpour ◽  
Zeinab Sadat Hosseiny ◽  
Zeinab Sadat Hosseiny ◽  
...  
Keyword(s):  
Gene Expression ◽  
Diabetes Mellitus ◽  
Group Treatment ◽  
Embryo Implantation ◽  
Treated Group ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Control Group ◽  
Genes Expression ◽  
Normal Menstrual Cycle

Background: Diabetes mellitus deeply changes the genes expression of integrin (Itg) subunits in several cells and tissues such as monocytes, arterial endothelium, kidney glomerular cells, retina. Furthermore, hyperglycemia could impress and reduce the rate of successful assisted as well as non-assisted pregnancy. Endometrium undergoes thorough changes in normal menstrual cycle and the question is: What happens in the endometrium under diabetic condition? Objective: The aim of the current study was to investigate the endometrial gene expression of α3, α4, αv, Itg β1 and β3 subunits in diabetic rat models at the time of embryo implantation. Materials and Methods: Twenty-eight rats were randomly divided into 4 groups: control group, diabetic group, pioglitazone-treated group, and metformin-treated group. Real-time PCR was performed to determine changes in the expression of Itg α3, α4, αv, β1, and β3 genes in rat’s endometrium. Results: The expression of all Itg subunits increased significantly in diabetic rats’ endometrium compared with control group. Treatment with pioglitazone significantly reduced the level of Itg subunits gene expression compared with diabetic rats. While metformin had a different effect on α3 and α4 and elevated these two subunits gene expression. Conclusion: Diabetes mellitus significantly increased the expression of studied Itg subunits, therefore untreated diabetes could be potentially assumed as one of the preliminary elements in embryo implantation failure.

scholarly journals Identification of gene expression and DNA methylation of SERPINA5 and TIMP1 as novel prognostic markers in lower-grade gliomas

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9262
Author(s):  
Wen-Jing Zeng ◽  
Yong-Long Yang ◽  
Zhi-Peng Wen ◽  
Peng Chen ◽  
Xiao-Ping Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Classification System ◽  
Differentially Expressed ◽  
Integrated Analysis ◽  
Lower Grade ◽  
Kaplan Meier ◽  
Genome Wide ◽  
Genes Expression ◽  
Tumors Classification ◽  
Long Term Survivors

Background Lower-grade gliomas (LGGs) is characteristic with great difference in prognosis. Due to limited prognostic biomarkers, it is urgent to identify more molecular markers to provide a more objective and accurate tumor classification system for LGGs. Methods In the current study, we performed an integrated analysis of gene expression data and genome-wide methylation data to determine novel prognostic genes and methylation sites in LGGs. Results To determine genes that differentially expressed between 44 short-term survivors (<2 years) and 48 long-term survivors (≥2 years), we searched LGGs TCGA RNA-seq dataset and identified 106 differentially expressed genes. SERPINA5 and TIMP1 were selected for further study. Kaplan–Meier plots showed that SERPINA5 and TIMP1 expression were significantly correlated with overall survival (OS) and relapse-free survival (RFS) in TCGA LGGs patients. We next validated the correlation between the candidate genes expression and clinical outcome in CGGA LGGs patients. Multivariate analysis showed that TIMP1 mRNA expression had a significant prognostic value independent of other variables (HR = 4.825, 95% CI = 1.370–17.000, P = 0.014). Then, differential methylation sites were identified from differentially candidate gene expression groups, and all four methylation sites were significantly negatively correlated with gene expression (spearman r <  − 0.5, P < 0.0001). Moreover, hyper-methylation of four methylation sites indicated better OS (P < 0.05), and three of them also shown statistical significantly association with better RFS, except for SERPINA5 cg15509705 (P = 0.0762). Conclusion Taken together, these findings indicated that the gene expression and methylation of SERPINA5 and TIMP1 may serve as prognostic predictors in LGGs and may help to precise the current histology-based tumors classification system and to provide better stratification for future clinical trials.

A 5.5 megapixel high-performance low-light military video camera

2009 ◽  
Author(s):  
Gerald B. Heim ◽  
Brian Biesterfeld ◽  
Jon Burkepile ◽  
Wayne W. Frame ◽  
Tyson Harding ◽  
...  
Keyword(s):  
High Performance ◽  
Video Camera ◽  
Low Light
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