Study on Synergistic Antimicrobial Effect of Origanum Vulgare L and Forsythia suspensa Essential Oils

2011 ◽  
Vol 236-238 ◽  
pp. 2332-2335
Author(s):  
Ren Yong Gu ◽  
Ai Hua Zhu ◽  
Li Zhang
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Antimicrobial Effect ◽  
Dilution Method ◽  
Fractional Inhibitory Concentration ◽  
Origanum Vulgare ◽  
Combined Use ◽  
Forsythia Suspensa ◽  
Carbon Dioxide Extraction

Supercritical carbon dioxide extraction (SCDE) technique was used to extract essential oils of Origanum vulgare L and Forsythia suspensa. The minimum inhibitory concentrations (MIC) of Origanum vulgare L essential oil and Forsythia suspensa essential oil used alone and in combination were determined by the same geometric dilution method, respectively. Then the synergistic antimicrobial effect of two essential oils was evaluated according to the calculated FIC values. The results showed that in light of the criteria of fractional inhibitory concentration (FIC), the combined use of Origanum vulgare L and Forsythia suspensa essential oils had synergistic effect on three bacteria (FIC=0.2-0.4) and on two mildews (FIC=0.1), and additive effect on two yeasts (FIC=0.8).

Antimicrobial Activity of Individual and Combined Essential Oils against Foodborne Pathogenic Bacteria

2016 ◽  
Vol 79 (2) ◽  
pp. 309-315 ◽  
Author(s):  
FATIMA REYES-JURADO ◽  
AURELIO LÓPEZ-MALO ◽  
ENRIQUE PALOU
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Synergistic Effects ◽  
Gas Chromatography Mass Spectrometry ◽  
Thymus Vulgaris ◽  
Chemical Compositions ◽  
Fractional Inhibitory Concentration ◽  
Mustard Essential Oil

ABSTRACTThe antimicrobial activities of essential oils from Mexican oregano (Lippia berlandieri Schauer), mustard (Brassica nigra), and thyme (Thymus vulgaris) were evaluated alone and in binary combinations against Listeria monocytogenes, Staphylococcus aureus, or Salmonella Enteritidis. Chemical compositions of the essential oils were analyzed by gas chromatography–mass spectrometry. The MICs of the evaluated essential oils ranged from 0.05 to 0.50% (vol/vol). Mustard essential oil was the most effective, likely due to the presence of allyl isothiocyanate, identified as its major component. Furthermore, mustard essential oil exhibited synergistic effects when combined with either Mexican oregano or thyme essential oils (fractional inhibitory concentration indices of 0.75); an additive effect was obtained by combining thyme and Mexican oregano essential oils (fractional inhibitory concentration index = 1.00). These results suggest the potential of studied essential oil mixtures to inhibit microbial growth and preserve foods; however, their effect on sensory quality in selected foods compatible with their flavor needs to be assessed.

scholarly journals TheIn VitroAntimicrobial Activity ofLavandula angustifoliaEssential Oil in Combination with Other Aroma-Therapeutic Oils

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Stephanie de Rapper ◽  
Guy Kamatou ◽  
Alvaro Viljoen ◽  
Sandy van Vuuren
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Citrus Sinensis ◽  
Inhibitory Concentration ◽  
Fractional Inhibitory Concentration ◽  
Ionization Detector ◽  
Cinnamomum Zeylanicum ◽  
Oil Blends ◽  
Flame Ionization

The antimicrobial activity ofLavandula angustifoliaessential oil was assessed in combination with 45 other oils to establish possible interactive properties. The composition of the selected essential oils was confirmed using GC-MS with a flame ionization detector. The microdilution minimum inhibitory concentration (MIC) assay was undertaken, whereby the fractional inhibitory concentration (ΣFIC) was calculated for the oil combinations. When lavender oil was assayed in 1 : 1 ratios with other oils, synergistic (26.7%), additive (48.9%), non-interactive (23.7%), and antagonistic (0.7%) interactions were observed. When investigating different ratios of the two oils in combination, the most favourable interactions were whenL. angustifoliawas combined withCinnamomum zeylanicumor withCitrus sinensis,againstC. albicansandS. aureus,respectively. In 1 : 1 ratios, 75.6% of the essential oils investigated showed either synergistic or additive results, lendingin vitrocredibility to the use of essential oil blends in aroma-therapeutic practices. Within the field of aromatherapy, essential oils are commonly employed in mixtures for the treatment of infectious diseases; however, very little evidence exists to support the use in combination. This study lends some credence to the concomitant use of essential oils blended with lavender.

scholarly journals α-Terpinyl Acetate: Occurrence in Essential Oils Bearing Thymus pulegioides, Phytotoxicity, and Antimicrobial Effects

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1065
Author(s):  
Vaida Vaičiulytė ◽  
Kristina Ložienė ◽  
Jurgita Švedienė ◽  
Vita Raudonienė ◽  
Algimantas Paškevičius
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Poa Pratensis ◽  
Antimicrobial Effect ◽  
Forage Legume ◽  
Economic Productivity ◽  
Microdilution Method ◽  
Broth Microdilution Method ◽  
Phytotoxic Effect ◽  
Lower Effect

The aim of this study was to evaluate occurrence of T. pulegioides α-terpinyl acetate chemotype, as source of natural origin α-terpinyl acetate, to determine its phytotoxic and antimicrobial features. Were investigated 131 T. pulegioides habitats. Essential oils were isolated by hydrodistillation and analyzed by GC-FID and GC-MS. Phytotoxic effect of essential oil of this chemotype on monocotyledons and dicotyledons through water and air was carried out in laboratory conditions; the broth microdilution method was used to screen essential oil effect against human pathogenic microorganisms. Results showed that α-terpinyl acetate was very rare compound in essential oil of T. pulegioides: it was found only in 35% of investigated T. pulegioides habitats. α-Terpinyl acetate (in essential oil and pure) demonstrated different behavior on investigated plants. Phytotoxic effect of α-terpinyl acetate was stronger on investigated monocotyledons than on dicotyledons. α-Terpinyl acetate essential oil inhibited seeds germination and radicles growth for high economic productivity forage grass monocotyledon Poa pratensis, but stimulated seed germination for high economic productive forage legume dicotyledon Trifolium pretense. α-Terpinyl acetate essential oil showed high antimicrobial effect against fungi and dermatophytes but lower effect against bacteria and Candida yeasts. Therefore, T. pulegioides α-terpinyl acetate chemotype could be a potential compound for developing preventive measures or/and drugs for mycosis.

scholarly journals Evaluation of Antibacterial Effect againstPorphyromonas gingivalisand Biocompatibility of Essential Oil Extracted from the Gum ofPistacia atlantica Kurdica

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Shokhan H. Azeez ◽  
Shanaz M. Gaphor
Keyword(s):  
Wound Healing ◽  
Essential Oil ◽  
Wistar Rats ◽  
Antibacterial Effect ◽  
Subcutaneous Tissue ◽  
Dorsal Surface ◽  
Antimicrobial Effect ◽  
Histological Evaluation ◽  
Dilution Method ◽  
Molecular Technique

Objective. This study investigates the antibacterial effect of essential oil extracted from the gum ofPistacia atlantica KurdicaagainstPorphyromonas gingivalisand histological evaluation of an experimental gel of the extract in wound healing in the back subcutaneous tissue of Wistar rats.Methods. Clinical strains of bacteria obtained from subgingival plaque samples of individuals having periodontitis, isolation, and confirmation were done by conventional microbiological tests and molecular technique. Essential oil was extracted by using hydrodistillation method; antibacterial activity has been determined by two fold serial dilution method. Histological evaluation conducted on fifteen Wistar rats. Incisions were made on the dorsal surface of each animal for implanting of 3 polysilicone tubes (empty, tetracycline gel, and the experimental gel). After 1, 4, and 8 weeks, the animals were euthanized and the specimens were prepared histologically.Result. The extract demonstrated antimicrobial effect and significant wound healing in the different study durations particularly our product showed progression in epidermal wound healing and decrease in cellularity and scoring of inflammatory cells.Conclusion. The extract was able to pose inhibitory and bactericidal activity againstP. gingivalisand the experimental gel was able to have a good wound healing that enable it to be considered as a compatible material.

scholarly journals Chemical Composition and Antifungal Activity of the Essential Oils of Schinus weinmannifolius Collected in the Spring and Winter

2014 ◽  
Vol 9 (9) ◽  
pp. 1934578X1400900
Author(s):  
Camila Hernandes ◽  
Silvia H. Taleb-Contini ◽  
Ana Carolina D. Bartolomeu ◽  
Bianca W. Bertoni ◽  
Suzelei C. França ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Biological Activities ◽  
Antimicrobial Action ◽  
Pharmacological Profile

Reports on the chemical and pharmacological profile of the essential oil of Schinus weinmannifolius do not exist, although other Schinus species have been widely investigated for their biological activities. This work aimed to evaluate the chemical composition and antimicrobial activity of the essential oil of S. weinmannifolius collected in the spring and winter. The essential oils were extracted by hydrodistillation, analyzed by GC/MS and submitted to microdilution tests, to determine the minimum inhibitory concentration. The oils displayed different chemical composition and antimicrobial action. Bicyclogermacrene and limonene predominated in the oils extracted in the winter and spring, respectively, whereas only the latter oil exhibited antifungal activity.

Physicochemical and Antimicrobial Properties of Hydroxypropyl Methylcellulose-Cinnamon Essential Oil Emulsion: Effects of Micro- and Nanodroplets

2019 ◽  
Vol 15 (9) ◽  
Author(s):  
Shuzhi Li ◽  
Jingyang Zhou ◽  
Yanan Wang ◽  
Anguo Teng ◽  
Kai Zhang ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Food Processing ◽  
Antibacterial Agent ◽  
Inhibitory Concentration ◽  
Hydroxypropyl Methylcellulose ◽  
Antimicrobial Properties ◽  
Oil Emulsion ◽  
Oil Emulsions ◽  
Cinnamon Essential Oil

AbstractEmulsion is an efficient encapsulation tool for enhancing the functional properties of essential oils (EOs). Herein, two two-dimensional cinnamon essential oil emulsions (from micro- to nanoscales) were emulsified by hydroxypropyl methylcellulose, and their antimicrobial and physicochemical properties were investigated. For the models of Escherichia coli CGMCC 1.0907, Salmonella enterica subsp. Enterica serovar Typhi (CICC 10867), Staphylococcus aureus CGMCC 1.0089, and Listeria monocytogene CGMCC 1.9144, the minimum inhibitory concentration (MIC) of the nanoemulsion was 31.25 μL/mL compared to that of the microemulsion (62.5–125 μL/mL) and pure oil (125–250 μL/mL), indicating the superiority of nanoemulsion as an antibacterial agent. The results showed that the highest activity was seen in the gram-positive L. monocytogenes whereas the lowest was in the gram-negative S. enterica. The identified properties of HPMC (hydroxypropyl methylcellulose) provide the potential for emulsifying and enhancing essential oils in light industries, especially for food processing.

Application of essential oils as natural cosmetic preservatives

2013 ◽  
Vol 59 (4) ◽  
pp. 142-156 ◽  
Author(s):  
Mariola Dreger ◽  
Karolina Wielgus
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Contact Allergy ◽  
Skin Irritation ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Synergistic Activity ◽  
Minimum Concentration ◽  
Current State ◽  
Risk Benefit Assessment

Abstract Nowadays, safety of chemical preservatives has been questioned by a big number of consumers. Traditionally used preservatives often cause skin irritation and lead to allergenic reactions. Growing demands for more natural and preservative-free cosmetics promoted an idea of the replacement of synthetic preservatives with essential oils (EOs) of antimicrobial properties. The antimicrobial effect of essential oil depends on content, concentration and interactions between the main active compounds. Effective preservatives should be characterized by a broad spectrum of antimicrobial activity at a minimum concentration. Formulations containing both types of preservatives: essential oil and a synthetic one have been tested and proposed as a compromise that allows for reducing concentration of both components due to their synergistic activity. Although most essential oils are regarded as safe, some of them may cause risk of contact allergy or phototoxic reaction. A well balanced risk-benefit assessment of essential oils is one of the great challenges for scientists or health policy authorities. This paper presents current state of knowledge on essential oils focused on their antimicrobial properties, the assessment of their efficacy and safety as cosmetic preservatives.

scholarly journals The Chemical Composition and Anti-mycobacterial Activities of Trachyspermum copticum and Pelargonium graveolens Essential Oils

2020 ◽  
Vol 15 (1) ◽  
pp. 68-74 ◽  
Author(s):  
Jalil Kardan-Yamchi ◽  
Mohaddese Mahboubi ◽  
Hossein Kazemian ◽  
Gholamreza Hamzelou ◽  
Mohammad M. Feizabadi
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Chemical Composition ◽  
Minimum Inhibitory Concentration ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Mycobacterium Fortuitum ◽  
Drug Resistant ◽  
Pelargonium Graveolens ◽  
Trachyspermum Copticum

Background: Microbial resistance to antibiotics and their adverse effects related to these antibiotics are a matter of global public health in the 21th century. The emergence of drug-resistant strains, has gained the interest of the scientists to discover new antimicrobial agents from the essential oil of medicinal plants. Methods: Anti-mycobacterial effects of Trachyspermum copticum and Pelargonium graveolens essential oils were determined against multi-drug resistant clinical strains of Mycobacterium tuberculosis, Mycobacterium kansasii, Mycobacterium fortuitum and standard strain of Mycobacterium tuberculosis H37Rv by a Broth micro-dilution method. Pelargonium graveolens plant named Narmada was discovered by Kulkarni R.N et al. (Patent ID, USPP12425P2) and a formulation comprising thymol obtained from Trachyspermum is useful in the treatment of drug-resistant bacterial infections (Patent ID, US6824795B2). The chemical composition of hydro-distilled essential oils was determined by GC and GC-MS. Results: Minimum Inhibitory Concentration (MIC) values for T. copticum essential oil against tested isolates were ranged from 19.5 µg/mL to 78 µg/mL. The least minimum inhibitory concentration of P. graveolens extract against M. Kansasii and MDR-TB was 78 µg/ml. Conclusion: The results of the present research introduced T. copticum and P. graveolens essential oils as a remarkable natural anti-mycobacterial agent, but more pharmacological studies are required to evaluate their efficacy in animal models.

scholarly journals In VitroAntimicrobial Activity of Essential Oil ofThymus schimperi,Matricaria chamomilla,Eucalyptus globulus, andRosmarinus officinalis

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Awol Mekonnen ◽  
Berhanu Yitayew ◽  
Alemnesh Tesema ◽  
Solomon Taddese
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Drug Preparation ◽  
Bacteria And Fungi

In this study, thein vitroantimicrobial activities of four plant essential oils (T. schimperi,E. globulus,R. officinalis, andM. Chamomilla) were evaluated against bacteria and fungi. The studies were carried out using agar diffusion method for screening the most effective essential oils and agar dilution to determine minimum inhibitory concentration of the essential oils. Results of this study revealed that essential oils ofT. schimperi,E. globulus, andR. officinaliswere active against bacteria and some fungi. The antimicrobial effect ofM. chamomillawas found to be weaker and did not show any antimicrobial activity. The minimum inhibitory concentration values ofT. schimperiwere<15.75 mg/mL for most of the bacteria and fungi used in this study. The minimum inhibitory concentration values of the other essential oils were in the range of 15.75–36.33 mg/mL against tested bacteria. This study highlighted the antimicrobial activity of the essential oil ofE. globulus,M. chamomilla,T. Schimperi, andR. officinalis. The results indicated thatT. schimperihave shown strong antimicrobial activity which could be potential candidates for preparation of antimicrobial drug preparation.

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