Ultrasonic Extraction of Thai Propolis for Antimicrobial and Antioxidant Properties

2012 ◽  
Vol 506 ◽  
pp. 371-374 ◽  
Author(s):  
S. Sanpa ◽  
Krit Sutjarittangtham ◽  
T. Tunkasiri ◽  
Sukum Eitssayeam ◽  
P. Chantawannakul
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Extraction Methods ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Inhibitory Effects ◽  
Ultrasound Technology ◽  
Efficient Extraction

Propolis is the mixture of resins that honeybees collect from plant buds, leaves and exudates. Antimicrobial and antioxidant properties of propolis have been widely studied, however, there have few reports on Thai propolis. To increase the efficient extraction of organic propolis compounds, extraction methods have been developed. We applied the ultrasound technology to reduce extraction time and increase extraction yields of propolis. The propolis was extracted using ultrasound technology for 15-60 minutes and the propolis extracts were examined to compare their biological activities. Antimicrobial activities of the propolis were investigated using the agar well diffusion method. The free radical-scavenging and antioxidant activities of propolis extracts were measured using the DPPH and FolinCiocalteau colorimetric methods. The propolis extracted using ultrasound for 15 and 30 minutes showed inhibitory effects on tested fungi and bacteria as well as antioxidant activities.

Carbonic Anhydrase Inhibition Potential and Some Bioactivities of The Peripherally Tetrasubstituted Cobalt(II), Titanium(IV), Manganese(III) Phthalocyanines

2020 ◽  
Vol 17 ◽  
Author(s):  
Ayça Aktaş Karaçelik ◽  
Murat Küçük ◽  
Derya Efe ◽  
Volkan Çakır ◽  
Zekeriya Bıyıklıoğlu
Keyword(s):  
Antibacterial Activity ◽  
Carbonic Anhydrase ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Carbonic Anhydrase Activity ◽  
Diffusion Method ◽  
Antioxidant Power ◽  
Carbonic Anhydrase Inhibition

Background: Peripherally tetrasubstituted cobalt(II), titanium(IV), manganese(III) phthalocyanines carrying redox-active metal centers were previously synthesized. Metallophthalocyanines (MPcs) have exhibited the potential to use in medicinal applications due to biological activities such as antibacterial, antioxidant and enzyme inhibition. Objective: This study aim was to evaluate the previously synthesized metallophthalocyanines in terms of carbonic anhydrase inhibition, antioxidant, and antimicrobial activities. Methods: Carbonic anhydrase activity was assayed by following esterase activity. The antibacterial activity was tested by the disc diffusion method against eight bacteria. The antioxidant activity was determined by two common methods, 2,2- diphenyl-1-picrylhydrazyl (DPPH•) radical scavenging and ferric ion (III) reducing/antioxidant power (FRAP) assays. Results and Discussion: In this study, the inhibition potential of the metallophthallocyanines against carbonic anhydrase (CA) enzyme, which is important for treatments of many disorders, was evaluated. The metallophthalocyanines showed high CA inhibitory activity with IC50 values in the range of 74-317 nM, which was similar or better when compared with the standard CA inhibitors sulfanilamide and acetazolamide. In addition, antibacterial and antioxidant activities were determined. The metallophthallocyanines exhibited moderate antibacterial activity, especially against S. aureus and S. epidermis. The antioxidant activities of the compounds in both tests were quite high even exceeding the standards Trolox and BHT, with SC50 values of 0.0048-0.0257 and TEAC values of 1143.3-1543.7 µM, which meant 2 to 73 fold better activity. Conclusion: In conclusion, all three metallophthalocyanines exhibited excellent carbonic anhydrase and antioxidant potential and have deserved further interest in the synthesis of new derivatives.

Punica granatum as a Source of Natural Antioxidant and Antimicrobial Agent; a Comprehensive Review on Related Investigations

2020 ◽  
Vol 17 ◽  
Author(s):  
Atefeh Jalali ◽  
Mohammadreza Kiafar ◽  
Masih Seddigh ◽  
Mohammad M. Zarshenas
Keyword(s):  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Punica Granatum ◽  
Antimicrobial Activities ◽  
Natural Antioxidant ◽  
Antiviral Activities ◽  
Natural Foods

Background: The consumption of natural antioxidants is increasing due to the demand and tendency to natural foods. Punica granatum L. [Punicaceae] is a fruit with various bioactive ingredients. The effectiveness of this plant has been proved against various disorders such as hyperglycemia, hyperlipidemia, blood coagulation, infections, cancer, and dentistry. Among them, there are numerous researches on antimicrobial and antioxidant properties. Subsequently, the present study aimed to compile a review of those properties to outline this herb as a possible natural antioxidant and preservative. Methods: Synchronically, keywords "Punica granatum" with antimicrobial, or antibacterial, antifungal, antiviral, antioxidant and radical scavenging were searched through "Scopus" database up to 31st September 2019. Papers focusing on agriculture, genetics, chemistry, and environmental sciences were excluded and also related papers were collected. Results: Among 201 papers focusing on related activities, 111 papers have dealt with antioxidant activities focusing based on DPPH assay, 59 with antibacterial, on both gram+ and gram- bacteria, 24 with antifungal effects, mostly on Aspergillus niger and Candida albicans, and 7 papers with antiviral activities. There were about 50 papers focusing on in-vivo antioxidant activities of this plant. Conclusion: Taken together, botanical parts of P. granatum have possessed notable radical scavenging and antimicrobial activities that with these properties, this plant can be introduced as a natural safe source of preservative and antioxidant. Accordingly, P. granatum can be applied as excipient with the aforementioned properties in the pharmaceutical and food industries.

In Vitro Antimicrobial and Antioxidant Activities of Organic and Aqueous Extracts of Bacillus clausii KP10

2019 ◽  
Vol 41 (1) ◽  
pp. 161-161
Author(s):  
Farzana Nighat and Zahid Mushtaq Farzana Nighat and Zahid Mushtaq
Keyword(s):  
Hot Springs ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Aqueous Extracts ◽  
Current Case ◽  
Bacillus Clausii

Humans and plants are experiencing different kinds of diseases. Many of these diseases are mainly caused by microorganisms like fungi, bacteria and viruses that adversely affect their hosts. Bioactive compounds produced by bacteria can be used to treat the infections caused by these microorganisms. Free radicals that can cause serious damage to the cell can be repressed by antioxidants that are produced by bacteria as secondary compounds. The current case study was therefore planned to investigate the antimicrobial and antioxidant potentials of the organic and aqueous extracts of Bacillus clausii KP10 isolated recently by our group from hot springs of Tatta Pani, Poonch, Azad Kashmir. The antimicrobial activities were assessed using well diffusion method against two Gram positive bacteria; B. subtilis, and S. aureus, and two Gram negative bacteria; E. coli and P. multocida and three fungal strains; A. niger, A. parasiticus and A. ustus. Antioxidant activities of the extracts were done by DPPH free radical scavenging assay. In our experiments the maximum zones of inhibition were shown by ethanolic extract against P. multocida (21 mm), S. aureus (13 mm) and methanol soluble fraction showed zone of inhibition against A. ustus (21 mm) as compared to the standard ciprofloxacin for bacteria and as antifungal agents with terbinafine. In our studies n-hexane fraction of B. clausii KP10 showed maximum 88.67% antioxidant activity. Finally, results were analyzed statistically through analysis of variance (ANOVA). Hence it is proved that the bioactive fractions obtained from B. clausii KP10 are good source of antimicrobials and antioxidants and they can be used as medicines in future for the respective treatments.

scholarly journals Analysis of Phytoconstituents and Biological Activities on The Selected Medicinal Plants of Dolakha and Sindhualchowk District of Nepal

2018 ◽  
Vol 22 (2) ◽  
pp. 140-147 ◽  
Author(s):  
Bimala Subba ◽  
Susila Thapa
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Antioxidant Power ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Pharmacologically Active

Phytochemical and biological activities of methanolic extract of seven plants viz. Scindapsus officinalis Schott. , Lepisorus loriformis, Nicotiana tabacum L., Clematis buchananiana DC., Astilbe rivularis D. Don, Potentilla fulgens Wall. ex Hook and Taxus wallichiana Zucc. were carried out. The brine shrimp bioassay showed that N. tabacum, A. rivularis, and P. fulgens were pharmacologically active. The antibacterial potential was studied against Staphylococcus aureus (gram positive bacteria) and Escherichia coli (gram negative bacteria) using Agar Well Diffusion Method. Roots of P. fulgens showed inhibition against gram positive bacteria while the rhizome of A. rivularis showed inhibition against both gram positive and gram negative bacteria. Antioxidant activity was evaluated by 2, 2-diphenyl-1-picryl hydrazyl (DPPH) free radical scavenging activity and ferric reducing antioxidant power FRAP assay. Both assays showed that P. fulgens has high antioxidant activities with (IC50 =15.57 ± 3.6) lower than standard ascorbic acid.  Journal of Institute of Science and TechnologyVolume 22, Issue 2, January 2018, Page: 140-147

scholarly journals Free Radical Scavenging, Antimicrobial Activities and Effect of Sub-Acute Exposure to Nigerian Xylopia aethiopica Seed Extract on Liver and Kidney Functional Indices of Albino Rat

2018 ◽  
Vol 12 (3) ◽  
pp. 51-58
Author(s):  
Abubakar Awwal Yusuf ◽  
◽  
Bashir Lawal ◽  
Mumeen Abdulganeey Yusuf ◽  
Mumeen Abdulganeey Yusuf ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Seed Extract ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Frap Assay ◽  
Albino Rat ◽  
Liver And Kidney ◽  
Xylopia Aethiopica

Background: Xylopia aethiopica is highly reputed for its numerous medicinal properties. In the present study, antioxidant, antimicrobial, and toxicity profile of methanol seed extract of Nigerian X. aethiopica in rat were evaluated. Methods: Phytochemical compositions were evaluated using standard procedures. The antibacterial study was carried out using agar well diffusion method, while antioxidant activities were evaluated by DPPH and FRAP assay. Twenty-five rats (5 each) were given 0, 75, 150, 300 and 600 mg/kg bwt of the extract orally for 28 days. Results: The extract had total phenolic and total flavonoid contents of 15.98±0.03mg GAE/g and 2.29±0.02 mg/g CE respectively. The extract had IC50 values of 52.45±3.05 µg/mL and 73.45±3.89 μg/mL in DPPH and FRAP assay respectively. The E. coli showed the highest susceptibility (20.27±0.90mm) while P. aeuruginisa showed the least (15.08±0.20mm). The MIC ranged from 25-50 µg/mL while MBC ranged between 50µg/mL and 100 µg/mL. In comparison with the control rats, the levels of serum creatinine, bicarbonate total proteins, albumin, and ALP were significantly higher in rat dosed 600 mg/kg bwt while urea decreases in rat dose 300 and 600 mg/kg. However, serum concentration of ALT, AST, bilirubin, Na+, K+ and Cl- compared favorably (P>0.05) with control at all doses. Conclusion: The study revealed the antioxidant and antimicrobial activities of Nigerian X. aethiopica, the extract at 75, 150 and 300 mg/kg/b.wt did not provoke toxic effects to the animals’ liver and kidney; however, caution should be exercised when using as a prolonged oral remedy at high doses.

scholarly journals PHYTOCHEMICAL SCREENING, ANTIOXIDANT AND ANTIMICROBIAL ASSESSMENT OF PLUCHEA INDICA (L.) LESS EXTRACT AS AN ACTIVE INGREDIENT IN NATURAL LOTION BAR

2021 ◽  
pp. 51-57
Author(s):  
SIRIKHWAN TINRAT
Keyword(s):  
Broad Spectrum ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Antimicrobial Activities ◽  
Dilution Method ◽  
Reducing Ability ◽  
Phytochemical Compounds ◽  
Antioxidant And Antimicrobial Activities

Objective: To screen phytochemical compounds and study antioxidant and antimicrobial activities of Pluchea indica (L.) Less (PI) leaves extracts for assessment the moisturizing lotion bar formulation containing it’s as a bioactive ingredient. Methods: PI leaves were extracted in different solvents. Crude extracts were screened phytochemical compounds and evaluated antioxidant and antimicrobial activities before formulating to antibacterial moisturizing lotion bar. Results: 95% ethanolic extract displayed high antioxidant activities. DPPH radical scavenging capacity with IC50 was 6.80±0.04 mg/ml (61.37±0.41%) and the ferric reducing ability power was 381.20±0.10 mg AAE/100 gDW. Additionally, 95% ethanolic extract served as a broad spectrum bacteriostatic agents against gram-positive and gram-negative species with an inhibition zone of 6.50±0.00-46.67±0.58 mm. It also was simultaneously as a broad spectrum bactericidal agents (MIC and MBC value of 25-200 mg/ml) by broth micro-dilution method. The screened phytochemical constituents (saponins, tannins, flavonoids, terpenoids, steroids, and alkaloids) in PI leaves extract may be responsible for high biological activities. Thus, 95% ethanolic extract was the most appropriate bioactive ingredient for formulated the product development. Conclusion: The developed moisturizing lotion bar with 0.0041% of 95% ethanolic extract was the most eligible formula that showed good appearance, odor, color, homogeneity, after feel and stability. This extract-containing lotion bar formulation achieved a high level of customer satisfaction by sensory evaluation and development of this specific formulation should be subject to further clinical investigation.

scholarly journals Phytoconstituents and biological analysis of Acorus calamus rhizome of Sindhupalchowk District, Nepal

BIBECHANA ◽  
2020 ◽  
Vol 17 ◽  
pp. 104-109
Author(s):  
Shanta Pokhrel ◽  
Kalpana Chaulagain
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Acorus Calamus ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative

Phytochemical and biological activities of methanolic and hexane extract of Acorus calamus rhizome were carried out. Phytochemical analysis showed the presence of flavonoid, glycoside, saponin, resin and carbohydrates which are responsible for the antibacterial activities. The antibacterial potential was studied against Staphylococcus aureus (gram positive bacteria) and Escherichia coli, Salmonella typhi (gram negative bacteria) using Agar Well Diffusion Method. The activity was shown by both methanolic and hexane extract of Acorus calamus rhizome against both Gram positive and Gram negative bacteria. Antioxidant activity of methanolic extract was evaluated by 2, 2-diphenyl-1-picryl hydrazyl (DPPH) free radical scavenging activity showed potent antioxidant activities with IC50 value 3.74 μg/mL slightly higher than standard ascorbic acid (IC50 = 3.56 μg/mL). BIBECHANA 17 (2020) 104-109

scholarly journals Evaluation of Phytochemical, Antimicrobial and Antioxidant Capacities of Pennisetum purpureum (Schumach) Extracts

2020 ◽  
pp. 1-14
Author(s):  
I. R. Jack ◽  
P. D. Clark ◽  
G. I. Ndukwe
Keyword(s):  
Antifungal Activity ◽  
Vitamin C ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Antimicrobial Activities ◽  
Pennisetum Purpureum ◽  
Diffusion Method ◽  
Methanolic Extracts ◽  
Test Organisms

Medicinal plants are valuable natural sources for the development of potentially safe drugs. The biological activities associated with these plants are due to the presence of certain phytochemicals that act individually or synergistically. Thus, this study examined the phytochemical components, antioxidant and antimicrobial activities of n-hexane, ethylacetate and methanolic extracts of Pennisetum purpureum (Schumach). Qualitative and quantitative phytochemical assays of P. purpureum showed the presence of alkaloids (0.004%), saponins (0.002%), flavonoids (0.021%), steroids, terpenoids and glycosides (0.008%). Methanol, n-hexane and ethylacetate extracts of P. purpureum were examined for antimicrobial activity using the disc diffusion method. Six microbial strains were exposed to six different concentrations of each extracts; 200 mg/ml, 100 mg/ml, 50 mg/ml, 25 mg/ml, 12.5 mg/ml and 6.25 mg/ml. The three extracts demonstrated varied concentration-dependent antimicrobial activities against the test organisms. The methanolic extract showed antibacterial activity against E. coli, S. aureus, B. cereus and antifungal activity against T. mentagrophyte and A. niger. Among all extracts, the methanolic extract of P. purpureum exhibited relatively strong antifungal activity against A. niger (10.3±0.12 mm) when compared to the standard antifungal agent, fluconazole (13.9±0.12 mm). Furthermore, antioxidant activities were spectrophotometrically studied using vitamin C as standard; methanol and ethylacetate extracts of P. purpureum showed pronounced scavenging activity on 2,2-diphenyl-1-picrylhydrazyl (DPPH); and had a potent reductive ability on ferric ion and phosphomolybdate. However, only the non-polar extract of P. purpureum showed a non-significant correlation and significant differences when compared to vitamin C. Antioxidant activities of the plant extracts were observed in the order of methanol >ethylacetate> n-hexane extracts. The results showed that P. purpureum contains phytochemicals that significantly contributed to the observed antimicrobial and antioxidant abilities of the plant and could be used as a potential source for the development of novel therapeutic drugs.

scholarly journals Tunisian Native Mentha pulegium L. Extracts: Phytochemical Composition and Biological Activities

Molecules ◽  
2022 ◽  
Vol 27 (1) ◽  
pp. 314
Author(s):  
Jed Jebali ◽  
Hanene Ghazghazi ◽  
Chedia Aouadhi ◽  
Ines ELBini-Dhouib ◽  
Ridha Ben Salem ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Inhibition Zone ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Mentha Pulegium ◽  
Leaf Extracts ◽  
Traditional Remedies

Mint species (Lamiaceae family) have been used as traditional remedies for the treatment of several diseases. In this work, we aimed to characterize the biological activities of the total phenolic and flavonoid contents of Mentha pulegium L. extracts collected from two different regions of Tunisia. The highest amounts of total phenols (74.45 ± 0.01 mg GAE/g DW), flavonoids (28.87 ± 0.02 mg RE/g DW), and condensed tannins (4.35 ± 0.02 mg CE/g DW) were found in the Bizerte locality. Methanolic leaf extracts were subjected to HPLC-UV analysis in order to identify and quantify the phenolic composition. This technique allowed us to identify seven phenolic compounds: two phenolic acids and five flavonoid compounds, such as eriocitrin, hesperidin, narirutin, luteolin, and isorhoifolin, which were found in both extracts with significant differences between samples collected from the different regions (p < 0.05). Furthermore, our results showed that the methanolic extract from leaves collected from Bizerte had the highest antioxidant activities (DPPH IC50 value of 16.31 μg/mL and 570.08 μmol Fe2+/g, respectively). Both extracts showed high radical-scavenging activity as well as significant antimicrobial activity against eight tested bacteria. The highest antimicrobial activities were observed against Gram-positive bacteria with inhibition zone diameters and MIC values ranging between 19 and 32 mm and 40 and 160 µg/mL, respectively. Interestingly, at 10 μg/mL, the extract had a significant effect on cell proliferation of U87 human glioblastoma cells. These findings open perspectives for the use of Mentha pulegium L. extract in green pharmacy, alternative/complementary medicine, and natural preventive therapies for the development of effective antioxidant, antibacterial, and/or antitumoral drugs.

scholarly journals Investigation of the Chemical Composition and Antioxidant and Antimicrobial Activities of Lobularia maritima: Potent Therapeutic Applications

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Soumaya Kouidhi ◽  
Oumaima Zidi ◽  
Soukaina Abdelwahed ◽  
Yasmine Souissi ◽  
Najla Trabelsi ◽  
...  
Keyword(s):  
Biological Activities ◽  
Antioxidant Properties ◽  
Extraction Methods ◽  
Antimicrobial Activities ◽  
Biological Properties ◽  
Aspergillus Ochraceus ◽  
Therapeutic Applications ◽  
Lobularia Maritima ◽  
Promising Source ◽  
Antioxidant And Antimicrobial Activities

Lobularia maritima, commonly known as sweet alyssum, is an annual ornamental halophyte widely spread along the Tunisian seashore. However, little is known about the phytochemical, antioxidant, and antimicrobial activities of Lobularia maritima. The present study aimed to investigate the potential biological properties of different parts (flowers, leaves, roots, and stems) of Tunisian L. maritima using diverse extraction methods. Extracts were then studied for their antioxidant properties, and the highest antioxidant activity was presented in the roots’ fractions. Added to this, flower, leaf, and root fractions showed interesting antimicrobial and antifungal activities against different Gram+ and Gram− bacteria and against Aspergillus ochraceus. Finally, the most active fractions (presenting the highest biological activities) were analyzed using silica gel purification and mass spectrometry coupled to gas chromatography (GC-MS) analysis, and different compounds were identified such as camphor, amide of oleic acids, tributyl acetylcitrate, betulinaldehyde, menthol, 1′-(butyn-3-one-1-yl)-, (1S, 2S, 5R), benzyl benzoate, 7-acetyl-6-ethyl-1,1,4,4-tetramethyltetralin, 2,4-heptadienal, (E,E), and nootkaton-11,12-epoxide. This work represents the first in-depth investigation of the content of bioactive compounds from Lobularia maritima. This species could potentially be a promising source of useful compounds for therapeutic applications.

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