Investigation of the Chemical Composition and Antioxidant and Antimicrobial Activities of Lobularia maritima: Potent Therapeutic Applications

Lobularia maritima, commonly known as sweet alyssum, is an annual ornamental halophyte widely spread along the Tunisian seashore. However, little is known about the phytochemical, antioxidant, and antimicrobial activities of Lobularia maritima. The present study aimed to investigate the potential biological properties of different parts (flowers, leaves, roots, and stems) of Tunisian L. maritima using diverse extraction methods. Extracts were then studied for their antioxidant properties, and the highest antioxidant activity was presented in the roots’ fractions. Added to this, flower, leaf, and root fractions showed interesting antimicrobial and antifungal activities against different Gram+ and Gram− bacteria and against Aspergillus ochraceus. Finally, the most active fractions (presenting the highest biological activities) were analyzed using silica gel purification and mass spectrometry coupled to gas chromatography (GC-MS) analysis, and different compounds were identified such as camphor, amide of oleic acids, tributyl acetylcitrate, betulinaldehyde, menthol, 1′-(butyn-3-one-1-yl)-, (1S, 2S, 5R), benzyl benzoate, 7-acetyl-6-ethyl-1,1,4,4-tetramethyltetralin, 2,4-heptadienal, (E,E), and nootkaton-11,12-epoxide. This work represents the first in-depth investigation of the content of bioactive compounds from Lobularia maritima. This species could potentially be a promising source of useful compounds for therapeutic applications.

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Ultrasonic Extraction of Thai Propolis for Antimicrobial and Antioxidant Properties

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.506.371 ◽

2012 ◽

Vol 506 ◽

pp. 371-374 ◽

Cited By ~ 2

Author(s):

S. Sanpa ◽

Krit Sutjarittangtham ◽

T. Tunkasiri ◽

Sukum Eitssayeam ◽

P. Chantawannakul

Keyword(s):

Antioxidant Activities ◽

Biological Activities ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Extraction Methods ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Inhibitory Effects ◽

Ultrasound Technology ◽

Efficient Extraction

Propolis is the mixture of resins that honeybees collect from plant buds, leaves and exudates. Antimicrobial and antioxidant properties of propolis have been widely studied, however, there have few reports on Thai propolis. To increase the efficient extraction of organic propolis compounds, extraction methods have been developed. We applied the ultrasound technology to reduce extraction time and increase extraction yields of propolis. The propolis was extracted using ultrasound technology for 15-60 minutes and the propolis extracts were examined to compare their biological activities. Antimicrobial activities of the propolis were investigated using the agar well diffusion method. The free radical-scavenging and antioxidant activities of propolis extracts were measured using the DPPH and FolinCiocalteau colorimetric methods. The propolis extracted using ultrasound for 15 and 30 minutes showed inhibitory effects on tested fungi and bacteria as well as antioxidant activities.

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Metabolomic Profiling and Biological Activities of Pleurotus columbinus Quél. Cultivated on Different Agri-Food Byproducts

Antibiotics ◽

10.3390/antibiotics10101245 ◽

2021 ◽

Vol 10 (10) ◽

pp. 1245

Author(s):

Paola Angelini ◽

Roberto Maria Pellegrino ◽

Bruno Tirillini ◽

Giancarlo Angeles Flores ◽

Husam B. R. Alabed ◽

...

Keyword(s):

Mass Spectrometry ◽

Biological Activities ◽

Biological Effects ◽

Antioxidant Properties ◽

Principal Component ◽

Antimicrobial Activities ◽

Biological Properties ◽

Ms Analysis ◽

Quantitative Hplc ◽

Mushroom Extracts

The genus Pleurotus (Fr.) P. Kumm (Pleurotaceae, Basidiomycota) comprises a cosmopolitan group of mushrooms highly appreciated for their nutritional value and health-promoting benefits. Despite there being many studies about the phytochemical composition of Pleurotus spp., there are very few reports dealing with the phytochemistry, antioxidant and antimicrobial activities of P. columbinus Quél. In this study, a mass spectrometry ultra-performance liquid chromatography mass spectrometry (UHPLC)-QTOF method, coupled with principal component analysis (PCA), was applied to the P. columbinus metabolome in order to investigate the influence of different agri-food residues as growth substrates for P. columbinus cultivation, on the bioactive chemical profile of fruiting bodies and evaluated their potential as antioxidants and antimicrobials. Additionally, a quantitative HPLC-DAD-MS analysis was conducted on phenolic and flavonoid compounds, that could explain, albeit partially, the observed biological effects of P. columbinus extracts. The qualitative metabolic profile identified 97 metabolites, whereas the quantitative HPLC-DAD-MS analysis confirmed the presence of phenolic and flavonoids, in the mushroom extracts, which also showed intrinsic scavenging/reducing and antimicrobial effects. The antibacterial effects were particularly evident against Escherichia coli, whereas Tricophyton and Aspergillus were the dermatophytes more sensitive to the mushroom extracts. The present study supports more in-depth investigations, aimed at evaluating the influence of growth substrate on P. columbinus antimicrobial and antioxidant properties. The extracts from P. columbinus revealed valuable sources of primary and secondary metabolites, thus suggesting potential applications in the formulation of food supplements with biological properties, above all in terms of antioxidant and antimicrobial properties.

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Chemical Composition and Antioxidant and Antimicrobial Activities of Wormwood (Artemisia absinthiumL.) Essential Oils and Phenolics

Journal of Chemistry ◽

10.1155/2015/804658 ◽

2015 ◽

Vol 2015 ◽

pp. 1-12 ◽

Cited By ~ 35

Author(s):

Kamel Msaada ◽

Nidhal Salem ◽

Olfa Bachrouch ◽

Slim Bousselmi ◽

Sonia Tammar ◽

...

Keyword(s):

Gas Chromatography ◽

Essential Oil ◽

Essential Oils ◽

Methanolic Extract ◽

Biological Activities ◽

Reducing Power ◽

Antimicrobial Activities ◽

Gas Chromatography Mass Spectrometry ◽

Oil Composition ◽

Antioxidant And Antimicrobial Activities

The aim of this study was to determine the chemical variability of wormwood extracts as affected by the growing region. Antioxidant and antimicrobial activities were also investigated. The essential oil composition variability ofA. absinthiumL. aerial parts collected from four different Tunisian regions was assessed by gas chromatography (GC/FID) and by gas chromatography mass spectrometry (GC/MS). In addition, total polyphenols, flavonoids, and condensed tannins as well as antioxidant, antibacterial, and antifungal activities of methanolic extract and essential oils were undertaken. Chromatographic analysis of wormwood essential oils showed the predominance of monoterpene hydrocarbons represented mainly by chamazulene. RP-HPLC analysis of wormwood methanolic extract revealed the predominance of phenolic acids. Antiradical activity was region-dependant and the methanolic extract of Bou Salem region has the strongest activity (CI50=9.38±0.82 µg/mL). Concerning the reducing power, the methanolic extract of Bou Salem, Jérissa, and Boukornine regions was more active than the positive control. Obtained results of antimicrobial activities showed that wormwood essential oil is endowed with important antibacterial activity which was strongly related to the organoleptic quality of oil which appeared strongly region-dependant.A. absinthiumL. EOs investigated are quite interesting from a pharmaceutical standpoint because of their biological activities.

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Volatile Compounds and Antioxidant and Antimicrobial Activities of Selected Citrus Essential Oils Originated from Nepal

Molecules ◽

10.3390/molecules26216683 ◽

2021 ◽

Vol 26 (21) ◽

pp. 6683

Author(s):

Devi Prasad Bhandari ◽

Darbin Kumar Poudel ◽

Prabodh Satyal ◽

Karan Khadayat ◽

Sital Dhami ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Citrus Sinensis ◽

Antioxidant Properties ◽

Antimicrobial Activities ◽

Gas Chromatography Mass Spectrometry ◽

Diffusion Method ◽

Citrus Species ◽

Citrus Peel ◽

Antioxidant And Antimicrobial Activities

Citrus species of plants are among the most commercially cultivated crops, mainly for their fruit. Besides, the generally consumed flesh inside the fruit, the peel is quite important too. Essential oils extracted from the peel have a history of being used by humankind for centuries. These essential oils are rich in antioxidants and antimicrobial agents. Comparative investigation of volatile constituents, and antioxidant and antimicrobial activities were undertaken. The essential oils were evaluated through gas chromatography-mass spectrometry (GC–MS), and enantiomeric composition by chiral GC–MS. Similarly, the antioxidant properties were evaluated by 2,2-diphenyl-1-picrylhydrazyl scavenging assay, and antimicrobial activities were assayed using the disk diffusion method. The highest extraction yield of 1.83% was observed in Citrus sinensis Osbeck. GC–MS analysis showed limonene (63.76–89.15%), γ-terpinene (0.24–6.43%), β-pinene (0.15–6.09%), linalool (0.35–3.5%), sabinene (0.77–2.17%), myrcene (0.74–1.75%), α-terpineol (0.28–1.15%), and α-pinene (0.2–0.58%) as the major constituents of the essential oil of the Citrus species studied. For the first time, through our study, chiral terpenoids have been observed from Citrus grandis Osbeck essential oil. The order of antioxidant activity is as follows: Citrus grandis Osbeck red flesh > Citrus reticulata Blanco > Citrus sinensis Osbeck > Citrus grandis Osbeck white flesh. Except for Citrus grandis Osbeck white flesh (52.34 µL/mL), all samples demonstrated stronger antioxidant activities than those of the positive control, quercetin (5.60 µL/mL). Therefore, these essential oils can be used as a safe natural antioxidant to prevent product oxidation. Likewise, citrus peel essential oil showed antimicrobial activity against tested bacterial strains, albeit marginal.

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Lactic Acid Bacteria Fermentation and Endopeptidase Treatment Improve the Functional and Nutritional Features of Arthrospira platensis

Frontiers in Microbiology ◽

10.3389/fmicb.2021.744437 ◽

2021 ◽

Vol 12 ◽

Author(s):

Michela Verni ◽

Cinzia Dingeo ◽

Carlo Giuseppe Rizzello ◽

Erica Pontonio

Keyword(s):

Amino Acids ◽

Antioxidant Properties ◽

Amino Acid Content ◽

Protein Digestibility ◽

Integrated Approach ◽

Arthrospira Platensis ◽

Antimicrobial Activities ◽

Penicillium Roqueforti ◽

Freeze Dried ◽

Antioxidant And Antimicrobial Activities

This study aimed at investigating the effect of fermentation and enzymatic treatment on the degree of proteolysis of wet (WB), dried at low temperature (DB), and freeze-dried Spirulina (LB) proteins that affect the nutritional (e.g., amino acid content and profiles, and protein digestibility) and functional (e.g., antioxidant and antimicrobial activities) properties. The desiccation treatments influenced the unprocessed Spirulina characteristics because, compared with that in WB, peptides and free amino acids content was 73% lower in DB and 34% higher in LB. An integrated approach, including chromatographic and electrophoresis analyses, was used to evaluate the effect of the different bioprocessing options on protein profiles, release of peptides and amino acids, and the overall protein digestibility. Compared with the application of fermentation with the selected Lactiplantibacillus plantarum T0A10, the treatment with the endopeptidase Alcalase®, alone or combined, determined the most intense proteolysis. Moreover, the treatment with Alcalase® of LB allowed the release of potentially bioactive compounds that are able to inhibit Penicillium roqueforti growth, whereas the combination of fermentation with L. plantarum T0A10 and Alcalase® treatment increased Spirulina antioxidant properties, as determined by the scavenging activity toward ABTS radical (up to 60%) and antimicrobial activity against food pathogen Escherichia coli.

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Potential Anticancer Properties and Mechanisms of Action of Formononetin

BioMed Research International ◽

10.1155/2019/5854315 ◽

2019 ◽

Vol 2019 ◽

pp. 1-11 ◽

Cited By ~ 1

Author(s):

Dongjun Jiang ◽

Azhar Rasul ◽

Rabia Batool ◽

Iqra Sarfraz ◽

Ghulam Hussain ◽

...

Keyword(s):

Trifolium Pratense ◽

Antioxidant Properties ◽

Cancer Therapeutics ◽

Biological Properties ◽

Anticancer Properties ◽

Biological Potential ◽

Pharmacological Potential ◽

Reported Data ◽

Pharmacologically Active ◽

Promising Source

Nature, a vast reservoir of pharmacologically active molecules, has been most promising source of drug leads for the cure of various pathological conditions. Formononetin is one of the bioactive isoflavones isolated from different plants mainly from Trifolium pratense, Glycine max, Sophora flavescens, Pycnanthus angolensis, and Astragalus membranaceus. Formononetin has been well-documented for its anti-inflammatory, anticancer, and antioxidant properties. Recently anticancer activity of formononetin is widely studied. This review aims to highlight the pharmacological potential of formononetin, thus providing an insight of its status in cancer therapeutics. Formononetin fights progression of cancer via inducing apoptosis, arresting cell cycle, and halting metastasis via targeting various pathways which are generally modulated in several cancers. Although reported data acclaims various biological properties of formononetin, further experimentation on mechanism of its action, medicinal chemistry studies, and preclinical investigations are surely needed to figure out full array of its pharmacological and biological potential.

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Bioactive compounds, antioxidant and antimicrobial activities of Arum maculatum leaves extracts as affected by various solvents and extraction methods

Food Science & Nutrition ◽

10.1002/fsn3.815 ◽

2019 ◽

Vol 7 (2) ◽

pp. 465-475 ◽

Cited By ~ 10

Author(s):

Reza Farahmandfar ◽

Reza Esmaeilzadeh Kenari ◽

Maryam Asnaashari ◽

Dina Shahrampour ◽

Tahmineh Bakhshandeh

Keyword(s):

Bioactive Compounds ◽

Extraction Methods ◽

Antimicrobial Activities ◽

Arum Maculatum ◽

Antioxidant And Antimicrobial Activities

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Cornus mas L. Stones: A Valuable by-Product as an Ellagitannin Source with High Antioxidant Potential

Molecules ◽

10.3390/molecules25204646 ◽

2020 ◽

Vol 25 (20) ◽

pp. 4646

Author(s):

Dominika Przybylska ◽

Alicja Z. Kucharska ◽

Iwona Cybulska ◽

Tomasz Sozański ◽

Narcyz Piórecki ◽

...

Keyword(s):

Biological Activities ◽

Antioxidant Properties ◽

Biological Properties ◽

Raw Material ◽

Hydrolyzable Tannins ◽

Cornus Mas ◽

Fruit Processing ◽

High Antioxidant Activity ◽

Total Polyphenolic Content ◽

First Time

The stone of Cornus mas L. remains the least known morphological part of this plant, whereas the fruit is appreciated for both consumption purposes and biological activity. The stone is considered to be a byproduct of fruit processing and very little is known about its phytochemical composition and biological properties. In this study, the complete qualitative determination of hydrolyzable tannins, their quantitative analysis, total polyphenolic content, and antioxidant properties of the stone of C. mas are presented for the first time. The 37 identified compounds included the following: various gallotannins (11), monomeric ellagitannins (7), dimeric ellagitannins (10), and trimeric ellagitannins (7). The presence of free gallic acid and ellagic acid was also reported. Our results demonstrate that C. mas stone is a source of various bioactive hydrolyzable tannins and shows high antioxidant activity which could allow potential utilization of this raw material for recovery of valuable pharmaceutical or nutraceutical substances. The principal novelty of our findings is that hydrolyzable tannins, unlike other polyphenols, have been earlier omitted in the evaluation of the biological activities of C. mas. Additionally, the potential recovery of these bioactive chemicals from the byproduct is in line with the ideas of green chemistry and sustainable production.

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Chemical Profiling and Biological Properties of Extracts from Different Parts of Colchicum Szovitsii Subsp. Szovitsii

Antioxidants ◽

10.3390/antiox8120632 ◽

2019 ◽

Vol 8 (12) ◽

pp. 632 ◽

Cited By ~ 1

Author(s):

Gabriele Rocchetti ◽

Biancamaria Senizza ◽

Gokhan Zengin ◽

Murat Ali Okur ◽

Domenico Montesano ◽

...

Keyword(s):

Biological Activities ◽

Radical Scavenging ◽

Reducing Power ◽

Extraction Methods ◽

Biological Properties ◽

Leaf Extracts ◽

Orthogonal Projections ◽

Antioxidant Power ◽

Latent Structures

Like other members of the Colchicum genus, C. szovitsii subsp. szovitsii is also of medicinal importance in Turkish traditional medicine. However, its biological properties have not been fully investigated. Herein, we focused on the evaluation of the in vitro antioxidant and enzyme inhibitory effects of flower, root and leaf extracts, obtained using different extraction methods. In addition, a comprehensive (poly)-phenolic and alkaloid profiling of the different extracts was undertaken. In this regard, ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) allowed us to putatively annotate 195 polyphenols and 87 alkaloids. The most abundant polyphenols were flavonoids (83 compounds), whilst colchicine and 2-demethylcolchicine were some of the most widespread alkaloids in each extract analyzed. However, our findings showed that C. szovitsii leaf extracts were a superior source of both total polyphenols and total alkaloids (being, on average 24.00 and 2.50 mg/g, respectively). Overall, methanolic leaf extracts showed the highest (p < 0.05) ferric reducing antioxidant power (FRAP) reducing power (on average 109.52 mgTE/g) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging (on average 90.98 mgTE/g). Interestingly, each C. szovitsii methanolic extract was more active than the water extracts when considering enzymatic inhibition such as against tyrosinase, glucosidase, and acetylcholinesterase (AChE). Strong correlations (p < 0.01) were also observed between polyphenols/alkaloids and the biological activities determined. Multivariate statistics based on supervised orthogonal projections to latent structures discriminant analysis (OPLS-DA) allowed for the detection of those compounds most affected by the different extraction methods. Therefore, this is the first detailed evidence showing that C. szovitsii subsp. szovitsii might provide beneficial effects against oxidative stress and the associated chronic diseases. Nevertheless, the detailed mechanisms of action need to be further investigated.

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Phytochemical characterization, and antioxidant and antimicrobial activities of white cabbage extract on the quality and shelf life of raw beef during refrigerated storage

RSC Advances ◽

10.1039/d0ra06727j ◽

2020 ◽

Vol 10 (68) ◽

pp. 41430-41442

Author(s):

Momna Rubab ◽

Ramachandran Chelliah ◽

Kandasamy Saravanakumar ◽

Jong-Rae Kim ◽

Daesang Yoo ◽

...

Keyword(s):

Phenolic Compounds ◽

Biological Activities ◽

Wide Spectrum ◽

Antimicrobial Activities ◽

Refrigerated Storage ◽

White Cabbage ◽

Brassica Vegetables ◽

Cabbage Extract ◽

Antioxidant And Antimicrobial Activities ◽

Quality And Shelf Life

Brassica vegetables are well-characterized, containing a wide-spectrum of phenolic compounds that are responsible for their diverse biological activities like antioxidant and antimicrobial activities.

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