Correlation Analysis between Single Nucleotide Polymorphism of Fatty Acid Synthase and Fatness Traits in Chickens

2012 ◽  
Vol 535-537 ◽  
pp. 2283-2286
Author(s):  
Yu Xiang Wang
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Fatty Acid ◽  
Fatty Acid Synthase ◽  
Abdominal Fat ◽  
Least Square ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Chicken Population ◽  
Body Weights ◽  
F2 Chicken Population

The F2 chicken population from China Agricultural University was selected to explore the correlation between single nucleotide polymorphism of fatty acid synthase (FAS) and fatness traits. The primers of FAS gene were designed according to the sequence of chicken fatty acid synthase gene (accession number: J02839). The PCR-SSCP was used for genotype analysis. The results showed that the insertion or deletion of the 41st intron (1418-1424 bp) in chicken FAS gene could result in AA, AB and BB genotypes. In addition, least square analysis revealed that the deletion or mutation insertion of the 41st intron (1418-1424 bp) in FAS gene could affect body weights, abdominal fat weights and abdominal fat ratios of chickens. Therefore, polymorphic loci can be used for marker-assisted selection breeding of chickens.

​Identification of Single Nucleotide Polymorphism (SNP) C-2161G in the Promoter Region of Prolactin Gene and its Association with Egg Production in Tellicherry Native Chicken

2021 ◽  
Author(s):  
Azhaguraja Manoharan ◽  
S. Sankaralingam ◽  
P. Anitha ◽  
Binoj Chacko ◽  
T.V. Aravindakshan
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Promoter Region ◽  
Egg Production ◽  
Rflp Analysis ◽  
Genotypic Frequency ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Chicken Population ◽  
Native Chicken ◽  
Prolactin Gene

Background: Prolactin is a physiological candidate gene which has significant effects on egg production in poultry. Also, it plays a major role on incubation behaviour (broodiness) in birds. The main objective of the present study was identification of single nucleotide polymorphism (SNP) C-2161G in the promoter region of prolactin gene and its association with egg production in Tellicherry native chicken population. Methods: A total of 200 blood samples were collected from the randomly selected birds of Tellicherry native chicken in All India Co-ordinated Research Project on Poultry improvement (AICRP) farm, Mannuthy, Thrissur, Kerala. Isolation of Genomic DNA was done and the isolated samples were subjected to polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analysis to identify the SNP C-2161G of prolactin gene. Result: On the basis of RFLP patterns, birds were designated with three different genotypes namely CC, CG and GG. The allelic and genotypic frequency was calculated. The observed genotypic frequency at the SNP site C-2161G was CC (0.61), CG (0.160) and GG (0.23) and the frequency of allele was 0.69 for C and 0.31 for G. The egg production was shown to be statistically similar for the genotypes of SNP C-2161G.

scholarly journals The Effect of Maternal Fatty Acid Desaturase Single Nucleotide Polymorphism rs174602 and Prenatal Supplementation with Docosahexaenoic Acid on the Offspring Metabolome

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1276-1276
Author(s):  
Sonia Tandon ◽  
Ines Gonzalez-Casanova ◽  
Albino Barraza-Villarreal ◽  
Isabelle Romieu ◽  
Hans Demmelmair ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Fatty Acid ◽  
Fatty Acid Metabolism ◽  
Acid Metabolism ◽  
High Resolution Mass Spectrometry ◽  
Interaction Analysis ◽  
Fatty Acid Desaturase ◽  
Pathway Enrichment Analysis ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide

Abstract Objectives To assess if maternal fatty acid desaturase (FADS) single nucleotide polymorphism (SNP) rs174602 modifies the effect of prenatal DHA supplementation on the offspring metabolome at age 3 months. Methods Data were obtained from POSGRAD, a double-blind randomized controlled trial in Mexico in which 1094 women received 400 mg/day of algal DHA or a placebo (corn and soy oil) from mid-gestation until delivery. Genotyping was performed using maternal blood samples collected from women at baseline. Using liquid chromatography with high-resolution mass spectrometry, untargeted metabolomics was performed on plasma samples obtained from a random subsample of 112 offspring of POSGRAD participants at 3 months of age. Discriminatory metabolic features were selected via linear regression (P < 0.05); false discovery rate (FDR) was controlled for using the Benjamini-Hochberg method (q = 0.2). Analyses were adjusted for infant sex. Effect modification by FADS SNP rs174602 was assessed using two-way analysis of variance. Pathway enrichment analysis was performed with Mummichog (P < 0.05). Subgroup analyses were performed by rs174602, where the study population was grouped into carriers (TT, TC; n = 70) and non-carriers (CC; n = 42) of the minor allele. Results We identified 279 metabolic features that differed significantly between infants whose mothers received prenatal DHA (n = 59) versus placebo (n = 53); however, zero features remained significant after FDR correction. In the DHA * SNP rs174602 interaction analysis, following FDR correction, 346 differentially expressed features were identified. In the subgroup analysis, positively enriched fatty acid metabolism and decreased amino acid and vitamin B3 metabolism pathways were seen among carriers in the DHA group compared to placebo, whereas differentially enriched TCA cycle and omega-6 fatty acid metabolism pathways were observed by treatment group among non-carriers. Conclusions Maternal SNP rs174602 modified the effect of prenatal DHA supplementation on the infant metabolome at 3 months of age, particularly with regards to fatty acid metabolism. These findings provide additional support for the suggestion that differences in FADS genotype may explain inconsistent results observed across DHA supplementation trials. Funding Sources NIH, Nutricia Foundation, Laney Graduate School, and Conacyt, Mexico.

scholarly journals Single nucleotide polymorphism discovery of peroxisome proliferatorsactivated receptors gamma gene and its association with carcass traits in duck

2008 ◽  
Vol 51 (3) ◽  
pp. 276-282 ◽  
Author(s):  
Y. Wu ◽  
X. Liu ◽  
S. Hou ◽  
J. Wang
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Critical Role ◽  
Single Strand Conformation Polymorphism ◽  
Ppar Gamma ◽  
Abdominal Fat ◽  
Single Nucleotide Polymorphism Discovery ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Weight Percentage ◽  
Ppar Γ

Abstract. Peroxisome proliferators-activated receptor gamma (PPAR-γ) is thought to play a critical role in glucose and lipid metabolism and in inflammation control. The objective of this study was to detect the single nucleotide polymorphism (SNPs) in whole coding regions of PPAR-gamma gene with single strand conformation polymorphism (SSCP) in Peking duck Z4, Z2, Hybrid and Cherry Valley duck populations. The results showed that there were two alleles and three genotypes in the experimental populations. And one novel polymorphism, a synonymous mutation G-to-T at 213 of 5th exon, was found. The birds with homozygote (TT) was highest for carcass weight, sebum weight, percentage of sebum, abdominal fat weight, percentage of abdominal fat and IMF in four duck populations; however, for breast muscular weight and carcass net weight, the birds with heterozygote (GT) was highest in them. The association analysis results showed that the SNP of the polymorphism of PPAR-γ gene was linked with potential major loci or genes affecting some growth and fatness traits.

scholarly journals Identification of Single Nucleotide Polymorphism and Association Analysis of Alpha 2-Heremans Schmid Glycoprotein (AHSG) Gene Related to Fatty Acid Traits in Sheep

2019 ◽  
pp. 351-360
Author(s):  
J. P. Munyaneza ◽  
A. Gunawan ◽  
R. R. Noor
Keyword(s):  
Fatty Acids ◽  
Single Nucleotide Polymorphism ◽  
Fatty Acid ◽  
Linolenic Acid ◽  
Arachidic Acid ◽  
Nervonic Acid ◽  
Nucleotide Polymorphisms ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Ahsg Gene

Fatty acid (FA) composition of meat is regulated by many genes. The aim of this study was to identify Single Nucleotide Polymorphism (SNP) of Alpha 2-Heremans Schmid Glycoprotein (AHSG) gene and analyze its association with fatty acid (FA) traits in lambs. The study used a total of 67 rams of 12 months with average body size of 25-30 kg, consisted of 20 heads of Javanese Fat-Tailed (JFT) sheep, 17 heads of Javanese Thin-Tailed (JTT) sheep, 10 heads of Composite Garut (CG) sheep, 10 heads of Compass Agrinak (CA) sheep and 10 heads of Barbados Black Belly Cross (BC) sheep. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) were used to identify the single nucleotide polymorphisms (SNP) of AHSG gene. Association of AHSG genotypes with fatty acid traits was performed using General Linear Model by SAS 9.4 program. The SNP of AHSG gene was polymorphic with three genotypes (GG, GA and AA). In combined population, the genotype frequency of GG, GA and AA were 0.25, 0.13 and 0.62, respectively. The Chi-square test revealed that the locus of AHSG (g. 198655287 (G>A) was in Hardy-Weinberg equilibrium, except in Composite Garut (CG), Compass Agrinak (CA) and Barbados Black Belly Cross (BC) sheep breeds. The g.198655287 (G>A) SNP of AHSG gene was significantly associated (P<0.05) with saturated fatty acid, including <a href="https://en.wikipedia.org/wiki/Capric_acid">capric acid (C10:0)</a>, palmitic acid (C16:0), heptadecanoic acid (17:0), <a href="https://en.wikipedia.org/wiki/Arachidic_acid">arachidic acid (C20:0)</a>, heneicosylic acid (C21:0), <a href="https://en.wikipedia.org/wiki/Behenic_acid">behenic acid (C22:0)</a>, <a href="https://en.wikipedia.org/wiki/Tricosylic_acid">tricosylic acid (C23:0)</a>, <a href="https://en.wikipedia.org/wiki/Lignoceric_acid">lignoceric acid (C24:0)</a>; with monounsaturated fatty acids, including <a href="https://en.wikipedia.org/wiki/Palmitoleic_acid">palmitoleic acid (C16:1)</a>; oleic acid (C18:1n9c); eicosenoic acid (C20:1); nervonic acid (C24:1) and with polyunsaturated fatty acids, including linoleic acid (C18:2n6c); γ-Linolenic acid; α-Linolenic acid; <a href="https://en.wikipedia.org/w/index.php?title=Eicosadienoic_acid&action=edit&redlink=1">eicosadienoic acid (C20:2)</a>; dihomo-gamma-linolenic acid; arachidonic acid; <a href="https://en.wikipedia.org/w/index.php?title=Docosadienoic_acid&action=edit&redlink=1">docosadienoic acid(C22:2)</a>; eicosapentanoic and docosahexaenoic acid. The SNP g. 198655287 (G>A) of AHSG gene may be a useful marker for selecting and producing sheep meat having desirable fatty acids.

Sign in / Sign up

Export Citation Format

Share Document