Design-Expert Optimization of Skin-Care Material Fabrication Process

Skin-care functional material was mainly produced by three aftertreatments, namely carboxylic acid, alkali and sericin. The emphasis of this study lies in the optimization of material fabrication process, which was developed based on Plackett-Burman design to determine the best impact factor for optimum skin-care effect. The experiment was carried out using Design-Expert 8.0.5 statistical tool. The results of optimization showed that moisture character has been improved markedly and the most 3 significant factors were selected from 6 impacts on moisture rate. This research suggested that fabrics with amino acids own the quality of skin-care effect, which moisture rate has been improved. Plackett-Burman design can successfully sift dominant factors, accordingly confirm the feasibility.

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Application of Plackett-Burman Design in Screening the Significant Factors Effecting Denitrification of Salinity Wastewater by Biofortification SBR

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.675-677.770 ◽

2014 ◽

Vol 675-677 ◽

pp. 770-773

Author(s):

Jing Tang ◽

Shan Shan Qu ◽

Kun You ◽

Jin Xiang Fu

Keyword(s):

Significant Influence ◽

High Salinity ◽

Ammonia Concentration ◽

Operating Parameters ◽

Critical Factors ◽

Burman Design ◽

Plackett Burman Design ◽

Salinity Wastewater ◽

Significant Factors ◽

Sludge Concentration

A Plackett–Burman design was employed to investigate the effect of various parameters on the denitrification effect of high salinity wastewater treated by biofortification SBR.The purposes of this study were to investigate the significance of pH, DO, C/N, the initial ammonia concentration, salinity, the amount of bacteria and sludge concentration (MLSS).Factors which have significant influence on the denitrification effect of biofortification SBR treatment are quickly screened and used to establish a mathematical model.The results of quick screening provide critical factors for the optimization of operating parameters.

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Optimization of Fungal Dextranase Production and Its Antibiofilm Activity, Encapsulation and Stability in Toothpaste

Molecules ◽

10.3390/molecules25204784 ◽

2020 ◽

Vol 25 (20) ◽

pp. 4784 ◽

Cited By ~ 1

Author(s):

Nucharee Juntarachot ◽

Duangporn Kantachote ◽

Sartjin Peerajan ◽

Sasithorn Sirilun ◽

Chaiyavat Chaiyasut

Keyword(s):

Dental Caries ◽

Yeast Extract ◽

Suitable Condition ◽

Alginate Beads ◽

Antibiofilm Activity ◽

Inoculum Age ◽

Burman Design ◽

Plackett Burman Design ◽

Plaque Biofilm ◽

Significant Factors

Dextranase catalyzes the degradation of the substrate dextran, which is a component of plaque biofilm. This enzyme is involved in antiplaque accumulation, which can prevent dental caries. The activity of crude dextranase from Penicillium roquefortii TISTR 3511 was assessed, and the maximum value (7.61 unit/g) was obtained at 37 °C and pH 6. The Plackett–Burman design was used to obtain significant factors for enhancing fungal dextranase production, and three influencing factors were found: Dextran, yeast extract concentration and inoculum age. Subsequently, the significant factors were optimized with the Box–Behnken design, and the most suitable condition for dextranase activity at 30.24 unit/g was achieved with 80 g/L dextran, 30 g/L yeast extract and five day- old inoculum. The use of 0.85% alginate beads for encapsulation exhibited maximum dextranase activity at 25.18 unit/g beads, and this activity was stable in toothpaste for three months of testing. This study explored the potential production of fungal dextranase under optimal conditions and its encapsulation using alginate for the possibility of applying encapsulated dextranase as an additive in toothpaste products for preventing dental caries.

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Understanding the quality of protein loaded PLGA nanoparticles variability by Plackett–Burman design

International Journal of Pharmaceutics ◽

10.1016/j.ijpharm.2009.12.040 ◽

2010 ◽

Vol 389 (1-2) ◽

pp. 186-194 ◽

Cited By ~ 95

Author(s):

Ziyaur Rahman ◽

Ahmed S. Zidan ◽

Muhammad J. Habib ◽

Mansoor A. Khan

Keyword(s):

Plga Nanoparticles ◽

Burman Design ◽

Plackett Burman Design

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Optimization of Culture Parameters for Expression of Recombinant Human Basic Fibroblast Growth Factor in Pichia pastoris Using Response Surface Methodology Combining with Plackett-Burman Design

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.647.456 ◽

2013 ◽

Vol 647 ◽

pp. 456-461 ◽

Cited By ~ 1

Author(s):

Rui Jiang ◽

Li Wei Sun ◽

Yu Zhao ◽

Kai Feng ◽

Shou Yan Chen

Keyword(s):

Response Surface Methodology ◽

Growth Factor ◽

Pichia Pastoris ◽

Fibroblast Growth Factor ◽

Fibroblast Growth ◽

Fermentation Time ◽

Initial Ph ◽

Burman Design ◽

Plackett Burman Design ◽

Significant Factors

In this study, we used plackett-burman design (PBD) and response surface methodology (RSM) methods to optimize media and culture conditions for expression of recombinant human basic fibroblast growth factor (rhbFGF) in Pichia pastoris in order to effectively enhance the production of rhbFGF. PBD was used to screen the most significant factors from nine important factors, which were fermentation time, methanol, temperature, initial pH, OD600 for inducement, concentrated volume, YNB peptone, and casein. Then RSM was further used to optimize the significant parameters. Our results had shown that three significant factors selected by PBD were temperature, fermentation time, and methanol. Base on the 3-factor-3-level central composite design (CCD) of RSM, the final optimized conditions were fermentation time 126.7h, methanol 1.3%, temperature 21.3°C, initial pH 6.0±0.1, OD600 for inducement 26, concentrated volume 100 ml, YNB 13.4%, peptone 4%, and casein 0.1%. The maximum production of rhbFGF was 302.4mg/L, which was 2 times higher than the production of rhbFGF reported in the previous studies. Those results provided the foundation for further researches on higher density cultivation condition of rhbFGF explored in the fermenter and accelerate the industrialization development of rhbFGF.

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Berberine as siderophore from Talaromyces trachyspermus: augmentation and characterization”.

10.1101/2021.04.13.439607 ◽

2021 ◽

Author(s):

Sharda Sahu ◽

ANIL Prakash

Keyword(s):

Response Surface Methodology ◽

Succinic Acid ◽

Central Composite Design ◽

Endophytic Fungus ◽

Scale Up ◽

Burman Design ◽

Plackett Burman Design ◽

Central Composite ◽

Important Drug ◽

Significant Factors

In the present study, a siderophore compound produced by an endophytic fungus, Talaromyces trachyspermus was optimized for maximum production, 88.9 % SU by applying Plackett-Burman design and Response Surface Methodology through Central Composite Design that showed the succinic acid (1.141 g/L), sucrose (31.028 g/L) and temperature (27.475 ºC) as significant factors. On scale up, a further increase in siderophore yield was obtained (by 3%) The compound was extracted, purified and detected chemically as catecholate siderophore showing max. λ absorbance at 279nm. Contained of hydroxy benzene as shown by GC-MS analysis and further identified as berberine by HRLC-MS studies. The compound berberine is clinically a very important drug with several ethnobotanical properties. This is rare to report fungal catecholate and first to report the production of berberine from Talaromyces species .In the present study, a siderophore compound produced by an endophytic fungus, Talaromyces trachyspermus was optimized for maximum production, 88.9 % SU by applying Plackett-Burman design and Response Surface Methodology through Central Composite Design that showed the succinic acid (1.141 g/L), sucrose (31.028 g/L) and temperature (27.475 ºC) as significant factors. On scale up, a further increase in siderophore yield was obtained (by 3%) The compound was extracted, purified and detected chemically as catecholate siderophore showing max. λ absorbance at 279nm. Contained of hydroxy benzene as shown by GC-MS analysis and further identified as berberine by HRLC-MS studies. The compound berberine is clinically a very important drug with several ethnobotanical properties. This is rare to report fungal catecholate and first to report the production of berberine from Talaromyces species .

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Preparation and formulation of transferosomes containing an antifungal agent for transdermal delivery: Application of Plackett-Burman design to identify significant factors influencing vesicle size

Journal of Pharmacy And Bioallied Sciences ◽

10.4103/0975-7406.94140 ◽

2012 ◽

Vol 4 (5) ◽

pp. 60 ◽

Cited By ~ 4

Author(s):

BRutu Patel ◽

RH Parikh

Keyword(s):

Transdermal Delivery ◽

Antifungal Agent ◽

Vesicle Size ◽

Factors Influencing ◽

Burman Design ◽

Plackett Burman Design ◽

Significant Factors

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Optimization of Lanthanide Borosilicate Frit Compositions for the Immobilization of Actinides Using a Plackett-Burman/Simplex Algorithm Design

MRS Proceedings ◽

10.1557/proc-465-105 ◽

1996 ◽

Vol 465 ◽

Cited By ~ 9

Author(s):

M. G. Mesko ◽

T. F. Meaker ◽

W. G. Ramsey ◽

J. C. Marra ◽

D. K. Peeler

Keyword(s):

Optimization Technique ◽

Algorithm Design ◽

The United States ◽

Simplex Algorithm ◽

Savannah River ◽

Statistical Tool ◽

Noticeable Improvement ◽

Burman Design ◽

The Individual ◽

Plackett Burman Design

ABSTRACTImmobilization by vitrification is one potential disposition option for a portion of the United States' excess plutonium inventory. Research has been performed at the Savannah River Site (SRS) to determine the optimum composition of a lanthanide borosilicate frit for the vitrification of plutonium using a Plackett-Burman design and simplex algorithm as a statistical tool. This technique uses various response variables to rank and optimize a composition. The variables used in this study correspond to homogeneity, durability, actinide solubility and devitrification after heat-treatment.The optimized frit composition was determined using a constant ThO2 loading of 20 wt%. No noticeable trends were followed with respect to the individual components which may indicate a relatively robust system able to accommodate variations in the feed.Batches containing various loadings of ThO2 were melted to determine if actinide solubility was improved in the optimized composition compared to that of a similar lanthanide borosilicate glass. No noticeable improvement in ThO2 solubility was realized as a result of using this optimization technique.

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Lactose Inducible Fermentation in Escherichia coli for Improved Production of Recombinant Urate Oxidase: Optimization by Statistical Experimental Designs

10.21203/rs.3.rs-93296/v1 ◽

2020 ◽

Author(s):

Hamid shahbaz mohammadi ◽

Abbas Najjari ◽

Eskandar Omidinia

Keyword(s):

Yeast Extract ◽

Urate Oxidase ◽

Optimum Conditions ◽

Iptg Induction ◽

Lactose Induction ◽

Induction Strategy ◽

Burman Design ◽

Plackett Burman Design ◽

Central Composite ◽

Significant Factors

Abstract The enzyme urate oxidase (UOX) is used as a drug for preventing and treatment of chemotherapy-induced hyperuricemia. This study deals with the statistical optimization of lactose inducible fermentation for production of soluble recombinant Aspergillus flavus UOX. 10 variables were investigated by Plackett–Burman design (PBD), and the most significant factors were further optimized by central composite design (CCD). PBD results indicated that glycerol, yeast extract, tryptone, and lactose affected UOX activity significantly. The CCD results showed that the maximum enzyme activity (19.34 U/ml) could be achieved under the optimum conditions of glycerol 0.87 g/L, yeast extract 9.11 g/L, tryptone 10.29 g/L, K2HPO4 1.81 g/L, and lactose 12.79 g/L. When the same induction strategy was tested at shake flask, 19.34 U/mL of UOX activity was obtained, which was 12.5 folds higher than IPTG induction protocol. Furthermore, the lower total cost (0.7 vs. 13.5 €) was additionally feature that confirmed the suitability of the lactose induction method. Collectively, our results showed that design of experiment methodology can be applied as a suitable tool for improved production of UOX using lactose as the inducer.

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