Application of Plackett-Burman Design in Screening the Significant Factors Effecting Denitrification of Salinity Wastewater by Biofortification SBR

2014 ◽  
Vol 675-677 ◽  
pp. 770-773
Author(s):  
Jing Tang ◽  
Shan Shan Qu ◽  
Kun You ◽  
Jin Xiang Fu
Keyword(s):  
Significant Influence ◽  
High Salinity ◽  
Ammonia Concentration ◽  
Operating Parameters ◽  
Critical Factors ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Salinity Wastewater ◽  
Significant Factors ◽  
Sludge Concentration

A Plackett–Burman design was employed to investigate the effect of various parameters on the denitrification effect of high salinity wastewater treated by biofortification SBR.The purposes of this study were to investigate the significance of pH, DO, C/N, the initial ammonia concentration, salinity, the amount of bacteria and sludge concentration (MLSS).Factors which have significant influence on the denitrification effect of biofortification SBR treatment are quickly screened and used to establish a mathematical model.The results of quick screening provide critical factors for the optimization of operating parameters.

scholarly journals Optimization of Fungal Dextranase Production and Its Antibiofilm Activity, Encapsulation and Stability in Toothpaste

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4784 ◽  
Author(s):  
Nucharee Juntarachot ◽  
Duangporn Kantachote ◽  
Sartjin Peerajan ◽  
Sasithorn Sirilun ◽  
Chaiyavat Chaiyasut
Keyword(s):  
Dental Caries ◽  
Yeast Extract ◽  
Suitable Condition ◽  
Alginate Beads ◽  
Antibiofilm Activity ◽  
Inoculum Age ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Plaque Biofilm ◽  
Significant Factors

Dextranase catalyzes the degradation of the substrate dextran, which is a component of plaque biofilm. This enzyme is involved in antiplaque accumulation, which can prevent dental caries. The activity of crude dextranase from Penicillium roquefortii TISTR 3511 was assessed, and the maximum value (7.61 unit/g) was obtained at 37 °C and pH 6. The Plackett–Burman design was used to obtain significant factors for enhancing fungal dextranase production, and three influencing factors were found: Dextran, yeast extract concentration and inoculum age. Subsequently, the significant factors were optimized with the Box–Behnken design, and the most suitable condition for dextranase activity at 30.24 unit/g was achieved with 80 g/L dextran, 30 g/L yeast extract and five day- old inoculum. The use of 0.85% alginate beads for encapsulation exhibited maximum dextranase activity at 25.18 unit/g beads, and this activity was stable in toothpaste for three months of testing. This study explored the potential production of fungal dextranase under optimal conditions and its encapsulation using alginate for the possibility of applying encapsulated dextranase as an additive in toothpaste products for preventing dental caries.

Parameter Screening for the Important Factors Influencing the As(V) Adsorption Using a Plackett-Burman Design

2014 ◽  
Vol 931-932 ◽  
pp. 178-182
Author(s):  
Nusavadee Pojananukij ◽  
Kitirote Wantala ◽  
Sutasinee Neramittagapong ◽  
Arthit Neramittagapong
Keyword(s):  
Aqueous Solution ◽  
Batch Reactor ◽  
Operating Parameters ◽  
Adsorption Efficiency ◽  
Initial Concentration ◽  
Factors Influencing ◽  
Adsorption Temperature ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Adsorbent Dose

The adsorption efficiency of As (V) ions from an aqueous solution was investigated. The adsorption experiments were carried out in a batch reactor. The effects of operating parameters; i.e. the absorbent dose (g/L), the pH, the adsorption temperature (°C), the As (V) initial concentration (ppb), and type of adsorbents, on the adsorption efficiencies were studied. A PlackettBurman experimental design was used to screen for the important factors that influence As (V) adsorption. It has been found that the most important effect on the As (V) adsorption capacity was the type of adsorbent. The factor importance could be written in descending order as follows: the type of adsorbents, the adsorbent dose, the As (V) initial concentration, the adsorption temperature, and the pH.

Optimization of Culture Parameters for Expression of Recombinant Human Basic Fibroblast Growth Factor in Pichia pastoris Using Response Surface Methodology Combining with Plackett-Burman Design

2013 ◽  
Vol 647 ◽  
pp. 456-461 ◽  
Author(s):  
Rui Jiang ◽  
Li Wei Sun ◽  
Yu Zhao ◽  
Kai Feng ◽  
Shou Yan Chen
Keyword(s):  
Response Surface Methodology ◽  
Growth Factor ◽  
Pichia Pastoris ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
Fermentation Time ◽  
Initial Ph ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Significant Factors

In this study, we used plackett-burman design (PBD) and response surface methodology (RSM) methods to optimize media and culture conditions for expression of recombinant human basic fibroblast growth factor (rhbFGF) in Pichia pastoris in order to effectively enhance the production of rhbFGF. PBD was used to screen the most significant factors from nine important factors, which were fermentation time, methanol, temperature, initial pH, OD600 for inducement, concentrated volume, YNB peptone, and casein. Then RSM was further used to optimize the significant parameters. Our results had shown that three significant factors selected by PBD were temperature, fermentation time, and methanol. Base on the 3-factor-3-level central composite design (CCD) of RSM, the final optimized conditions were fermentation time 126.7h, methanol 1.3%, temperature 21.3°C, initial pH 6.0±0.1, OD600 for inducement 26, concentrated volume 100 ml, YNB 13.4%, peptone 4%, and casein 0.1%. The maximum production of rhbFGF was 302.4mg/L, which was 2 times higher than the production of rhbFGF reported in the previous studies. Those results provided the foundation for further researches on higher density cultivation condition of rhbFGF explored in the fermenter and accelerate the industrialization development of rhbFGF.

scholarly journals Berberine as siderophore from Talaromyces trachyspermus: augmentation and characterization”.

2021 ◽  
Author(s):  
Sharda Sahu ◽  
ANIL Prakash
Keyword(s):  
Response Surface Methodology ◽  
Succinic Acid ◽  
Central Composite Design ◽  
Endophytic Fungus ◽  
Scale Up ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite ◽  
Important Drug ◽  
Significant Factors

In the present study, a siderophore compound produced by an endophytic fungus, Talaromyces trachyspermus was optimized for maximum production, 88.9 % SU by applying Plackett-Burman design and Response Surface Methodology through Central Composite Design that showed the succinic acid (1.141 g/L), sucrose (31.028 g/L) and temperature (27.475 ºC) as significant factors. On scale up, a further increase in siderophore yield was obtained (by 3%) The compound was extracted, purified and detected chemically as catecholate siderophore showing max. λ absorbance at 279nm. Contained of hydroxy benzene as shown by GC-MS analysis and further identified as berberine by HRLC-MS studies. The compound berberine is clinically a very important drug with several ethnobotanical properties. This is rare to report fungal catecholate and first to report the production of berberine from Talaromyces species .In the present study, a siderophore compound produced by an endophytic fungus, Talaromyces trachyspermus was optimized for maximum production, 88.9 % SU by applying Plackett-Burman design and Response Surface Methodology through Central Composite Design that showed the succinic acid (1.141 g/L), sucrose (31.028 g/L) and temperature (27.475 ºC) as significant factors. On scale up, a further increase in siderophore yield was obtained (by 3%) The compound was extracted, purified and detected chemically as catecholate siderophore showing max. λ absorbance at 279nm. Contained of hydroxy benzene as shown by GC-MS analysis and further identified as berberine by HRLC-MS studies. The compound berberine is clinically a very important drug with several ethnobotanical properties. This is rare to report fungal catecholate and first to report the production of berberine from Talaromyces species .

Design-Expert Optimization of Skin-Care Material Fabrication Process

2013 ◽  
Vol 746 ◽  
pp. 132-136 ◽  
Author(s):  
Yan Li Yan ◽  
Zhu Cui Yong ◽  
Jun Liu Guo ◽  
Yan Wei Chun
Keyword(s):  
Skin Care ◽  
Fabrication Process ◽  
Functional Material ◽  
Statistical Tool ◽  
Design Expert ◽  
Burman Design ◽  
Material Fabrication ◽  
Plackett Burman Design ◽  
Significant Factors

Skin-care functional material was mainly produced by three aftertreatments, namely carboxylic acid, alkali and sericin. The emphasis of this study lies in the optimization of material fabrication process, which was developed based on Plackett-Burman design to determine the best impact factor for optimum skin-care effect. The experiment was carried out using Design-Expert 8.0.5 statistical tool. The results of optimization showed that moisture character has been improved markedly and the most 3 significant factors were selected from 6 impacts on moisture rate. This research suggested that fabrics with amino acids own the quality of skin-care effect, which moisture rate has been improved. Plackett-Burman design can successfully sift dominant factors, accordingly confirm the feasibility.

scholarly journals Lactose Inducible Fermentation in Escherichia coli for Improved Production of Recombinant Urate Oxidase: Optimization by Statistical Experimental Designs

2020 ◽  
Author(s):  
Hamid shahbaz mohammadi ◽  
Abbas Najjari ◽  
Eskandar Omidinia
Keyword(s):  
Yeast Extract ◽  
Urate Oxidase ◽  
Optimum Conditions ◽  
Iptg Induction ◽  
Lactose Induction ◽  
Induction Strategy ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite ◽  
Significant Factors

Abstract The enzyme urate oxidase (UOX) is used as a drug for preventing and treatment of chemotherapy-induced hyperuricemia. This study deals with the statistical optimization of lactose inducible fermentation for production of soluble recombinant Aspergillus flavus UOX. 10 variables were investigated by Plackett–Burman design (PBD), and the most significant factors were further optimized by central composite design (CCD). PBD results indicated that glycerol, yeast extract, tryptone, and lactose affected UOX activity significantly. The CCD results showed that the maximum enzyme activity (19.34 U/ml) could be achieved under the optimum conditions of glycerol 0.87 g/L, yeast extract 9.11 g/L, tryptone 10.29 g/L, K2HPO4 1.81 g/L, and lactose 12.79 g/L. When the same induction strategy was tested at shake flask, 19.34 U/mL of UOX activity was obtained, which was 12.5 folds higher than IPTG induction protocol. Furthermore, the lower total cost (0.7 vs. 13.5 €) was additionally feature that confirmed the suitability of the lactose induction method. Collectively, our results showed that design of experiment methodology can be applied as a suitable tool for improved production of UOX using lactose as the inducer.

Assessment of liquid chromatographic method robustness by use of Plackett-Burman design

2010 ◽  
Vol 22 (2) ◽  
pp. 281-296 ◽  
Author(s):  
M. Mašković ◽  
B. Jančić-Stojanović ◽  
A. Malenović ◽  
D. Ivanović ◽  
M. Medenica
Keyword(s):  
Chromatographic Method ◽  
Liquid Chromatographic Method ◽  
Burman Design ◽  
Liquid Chromatographic ◽  
Plackett Burman Design
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