scholarly journals The optimization of fermentation conditions for producing cellulase of Bacillus amyloliquefaciens and its application to goose feed

2017 ◽  
Vol 4 (10) ◽  
pp. 171012 ◽  
Author(s):  
Miao Ye ◽  
Linghong Sun ◽  
Ru Yang ◽  
Zaigui Wang ◽  
KeZong Qi
Keyword(s):  
Incubation Time ◽  
Bacillus Amyloliquefaciens ◽  
Egg Production ◽  
Fertilization Rate ◽  
Culture Conditions ◽  
Liquid Volume ◽  
Hatching Rate ◽  
Culture Temperature ◽  
Egg Weight ◽  
Initial Ph

The proper culture conditions for producing cellulase of Bacillus amyloliquefaciens S1, isolated from the cecum of goose was optimized by single-factor experiment combined with orthogonal test. The properties of the cellulase were investigated by DNS method. The appropriate doses of B. amyloliquefaciens S1 were obtained by adding them to goose feed. It indicated that the suitable culture conditions of producing cellulase were the culture temperature of 37°C, the initial pH of 7.0, the incubation time of 72 h and the loaded liquid volume of 75 ml per 250 ml. The effects of each factor on producing cellulase by B. amyloliquefaciens S1 were as follows: initial pH > incubation time = culture temperature > loaded liquid volume. The optimum reaction temperature and pH were 50°C and 7.0, respectively. This enzyme is a kind of neutral cellulase that possesses resistance to heat and acidity. It showed high activity to absorbent cotton, soya bean meal and filter paper. By adding different doses of B. amyloliquefaciens S1 to the goose feed, it was found that the egg production, average egg weight, fertilization rate and the hatching rate were promoted both in experiment 1 (1.5 g kg −1 ) and experiment 2 (3 g kg −1 ). Also the difference of egg production, fertilization rate and hatching rate between experiment 1 and control group was obvious ( p  < 0.05), and the average egg weight was significantly increased in experiment 2 ( p  < 0.05).

scholarly journals Reproductive performance of asian catfish (Hemibagrus wyckii Bagridae), a candidate species for aquaculture

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 683 ◽  
Author(s):  
Netti Aryani ◽  
Indra Suharman ◽  
Hafrijal Syandri
Keyword(s):  
Reproductive Performance ◽  
Fertilization Rate ◽  
Culture Conditions ◽  
Hatching Rate ◽  
Male Fish ◽  
Egg Weight ◽  
Study Results ◽  
Candidate Species ◽  
Asian Catfish ◽  
Egg Diameter

Background: Hemibagrus wyckii Bagridae is one of the most important economic fish species that lives in the rivers and reservoir in Riau Province, Indonesia. The present study aimed to determine the reproductive performance of H.wyckii under culture conditions. Methods: A total of 10 female and 10 male fish were selected, and weight, length, characteristics of egg and sperm, and hatchery performance were measured. Eggs were fertilized using the dry method. Egg weight and egg diameters were measured for 50 eggs per female. Egg size (50 eggs for each fish) was measured using an Olympus microscope (CX40). Then, saline solution was added over the eggs, followed by the addition of pooled sperm from 10 males. Results: Average relative fecundity, egg weight and egg diameter were 2060±512 eggs/kg fish, 29.86±1.21 mg and 2.67±0.26 mm, respectively. The fertilization rate and hatching rate were 60.91±4.68% and 42.91±2.92% respectively. Sperm characteristics such as volume per fish (mL), pH, concentration (per mL), motility (%) and duration of motility (second) were 0.82±0.20, 7.15±0.12, 3.68±0.15, 72.77±1.46 and 47.5±4.84, respectively. Conclusion: The study results and scientific observations regarding reproductive performance suggest that H. wyckii can be considered a new candidate species for aquaculture.

scholarly journals Reproductive performance of asian catfish ( Hemibagrus wyckii Bleeker, 1858), a candidate species for aquaculture

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 683 ◽  
Author(s):  
Netti Aryani ◽  
Indra Suharman ◽  
Hafrijal Syandri
Keyword(s):  
Reproductive Performance ◽  
Fertilization Rate ◽  
Culture Conditions ◽  
Hatching Rate ◽  
Male Fish ◽  
Egg Weight ◽  
Study Results ◽  
Candidate Species ◽  
Asian Catfish ◽  
Egg Diameter

Background: Hemibagrus wyckii Bagridae is one of the important economic fish species that lives in the rivers and reservoir in Riau Province, Indonesia. The present study aimed to determine the reproductive performance of H.wyckii under culture conditions. Method s: A total of 10 female and 10 male fish were selected, and weight, length, characteristics of egg and sperm, and hatchery performance were measured. Eggs were fertilized using the dry method. Egg weight and egg diameters were measured for 50 eggs per female. Egg size (50 eggs for each fish) was measured using Labo microscope model  L-711 using software camera 3. Then, saline solution was added over the eggs, followed by the addition of pooled sperm from 10 males. Results: Average relative fecundity, egg weight and egg diameter were 2060±512 eggs/kg fish, 29.86±1.21 mg and 2.67±0.26 mm, respectively. The fertilization rate and hatching rate were 60.91±4.68% and 42.91±2.92% respectively. Sperm characteristics such as volume per fish (mL), pH, concentration (per mL), motility (%) and duration of motility (second) were 0.82±0.20, 7.15±0.12, 3.68±0.15, 72.77±1.46 and 47.5±4.84, respectively. Conclusion: The study results and scientific observations regarding reproductive performance suggest that H. wyckii can be considered a new candidate species for aquaculture.

Optimization of Culture Conditions for Biosynthesis of Lipopeptide by Bacillus Subtilis with Starch

2013 ◽  
Vol 291-294 ◽  
pp. 225-229 ◽  
Author(s):  
Zong Ming Zheng ◽  
Ti Peng Wang ◽  
Qin Wu
Keyword(s):  
Bacillus Subtilis ◽  
Culture Conditions ◽  
Culture Temperature ◽  
Ph Optimum ◽  
Linear Effect ◽  
Initial Ph ◽  
Culture Cycle ◽  
Optimization Of Culture Conditions ◽  
Fermentation Parameters ◽  
Main Effects

Optimization of culture conditions for lipopeptide production of lipopeptide by Bacillus subtilis NEL-01 was carried out in shaker flask batch fermentations using composite central design of response surface methodology (RSM). A five-level three-factor central composite design was employed to determine the maximum lipopeptide production at optimum levels for culture temperature, initial pH and culture cycle. Culture temperature and culture cycle showed the significant linear main effects, while pH had no significant linear effect. The production was also significantly affected by quadratic effect of culture temperature and initial pH. Optimum fermentation parameters were predicted at temperature, initial pH, and culture cycle of 34.81 °C, 7.33g/l, 49.26 h, respectively. The prediction lipopeptide yield was 1879.56 mg/l. The subsequent experiments confirmed the prediction.

Primary Study on Optimization of the Processing Technology in Aspergillus oryzae Seed Koji by Homogeneous Design

2013 ◽  
Vol 798-799 ◽  
pp. 1071-1074
Author(s):  
Ying Ying Ma ◽  
Zhong Kai Zhang ◽  
Xun Hai Pan ◽  
Jian Gang Yang
Keyword(s):  
Aspergillus Oryzae ◽  
Incubation Time ◽  
Culture Conditions ◽  
Wood Ash ◽  
Processing Technology ◽  
Primary Study ◽  
Culture Temperature ◽  
Culture Time ◽  
Spore Number

The homogeneous was used to optimize the processing technology of Aspergillus oryzae seed koji. The results show that the culture time is the most significant factor and the sequence of the residual factors are the square of culture time, the interaction between temperature and the time, the interaction of time and wood ash addition, the square of wood ash addition and the inoculation quantity. The results reveal that the optimized culture conditions are proved to be that the incubation time is 9 days, the inoculation quantity is 0.1×107/g, the dosage of wood ash is 1.267% and the culture temperature is 40°C. Under this condition, the prospective spore number of seed koji has reached to 1.89×109 /g.

scholarly journals DOMESTICATION OF FRESHWATER PUFFER FISH OR BUNTAL (Tetraodon palembangensis)

2008 ◽  
Vol 3 (2) ◽  
pp. 133
Author(s):  
I Wayan Subamia ◽  
Nina Meilisza ◽  
Sudarto Sudarto ◽  
Slamet Sugito
Keyword(s):  
Egg Production ◽  
Fertilization Rate ◽  
Reproductive Period ◽  
Hatching Rate ◽  
Good Growth ◽  
High Survival ◽  
Egg Hatching ◽  
Live Feed ◽  
Reproductive Ability ◽  
Puffer Fish

The Research Institute for Freshwater Ornamental Fish Culture has been conducting domestication research since 2004 on adult freshwater puffer fish or buntal broodstock in which the fish were reared in aquaria and fed with small feed fish teri (anchovy) and earthworm (Lumbricus sp.). The domestication of freshwater puffer fish or buntal has resulted in successful environmental adaptation and reproductive ability of the fish and further rearing of its fry. The adaptive ability was indicated by its high survival rate, good growth and development, and the reproductive success in spawning, egg production, hatching as well as growing of the larvae to fry stages. The mature gonad stage of the fish was reached at an average body weight of 150 g with a reproductive period of 5 months. The spawning occurred naturally and the eggs were gradually released at interval periods of 14—18 days with egg production rates of 1,000—1,900 eggs per spawning. The highest egg fertilization rate was 96%, with the highest egg hatching rate of 78.6%. Hatching process took place on the 6th to 13th days after spawning process. The surviving larvae began feeding filtered natural foods Moina sp. on the 3rd day up to the 6th day, whereas unfiltered Moina sp. was given from the 7th to 14th days. The fish were fed the live feed Culex sp. from the 15th to 30th days. In the following period the fish was fed earthworm and small feed fish teri (anchovy).

scholarly journals Kars İli Yetiştirici Koşullarındaki Kazların (Anser anser) Yumurta Verimi, Kuluçka, Büyüme, Kesim ve Karkas Özellikleri (I. Yumurta Verimi ve Kuluçka Özellikleri)

2019 ◽  
Vol 7 (3) ◽  
pp. 543 ◽  
Author(s):  
Kadir Önk ◽  
Turgut Kırmızıbayrak
Keyword(s):  
Maternal Age ◽  
Egg Production ◽  
Shape Index ◽  
Fertility Rate ◽  
Egg Laying ◽  
Hatching Rate ◽  
Anser Anser ◽  
Egg Weight ◽  
Family Enterprises ◽  
Effect Of Age

In order to determine egg production, yield records of 200 mature geese were used from 60 family enterprises. Total 2365 eggs were examined in order to determine the traits of egg yield. While examining mature geese to determine egg production and its traits, geese were grouped according to their feather color (white, yellow, black, piebald, and grey) and ages (1, 2, 3, 4, and >4). In determining the hatchability characteristics, age, feather color and egg weight (170.00). The general means of egg production and egg laying period was 12.66 ± 0.07 per geese and 28.61 ± 0.19 per day respectively, While the general means egg weight and egg shape index values were 163.74 ± 0.38 g and 65.78% respectively. The effect of age and feather color groups on egg production and laying period were significant. The general mean of natural hatchability traits of the geese eggs such as fertility rate, hatchability rate, hatching rate, embryonic mortality rate, and mortality in shell rate were found 76.10%, 60.88%, 80.00%, 13.16% and 6.84% respectively. While the effect of feather color on the natural hatching traits was insignificant, the effect of maternal age on fertility rate was significant but the effect on the other properties examined was insignificant. As a result, the egg production and laying period of Turkish domestic geese were lower than those of many goose breeds but egg weight was similar to many goose breeds. It was determined that hatching yield increased as egg weight increased. In terms of egg yield, it was determined that 3-year-old geese were higher than other age geese.

scholarly journals PENGARUH SUMBER SPERMATOFORA PADA INSEMINASI BUATAN INDUK BETINA UDANG WINDU TURUNAN PERTAMA (F-1) TERHADAP PEMIJAHAN, KUALITAS TELUR, DAN LARVA TURUNANNYA (F-2)

2019 ◽  
Vol 14 (2) ◽  
pp. 63
Author(s):  
Samuel Lante ◽  
Usman Usman
Keyword(s):  
Amino Acid ◽  
Artificial Insemination ◽  
Egg Production ◽  
Amino Acid Profile ◽  
Fertilization Rate ◽  
Hatching Rate ◽  
Wild Male ◽  
Tiger Shrimp ◽  
In The Wild ◽  
Egg Diameter

Penelitian ini bertujuan mengevaluasi pemijahan udang windu betina F-1 dan mutu telur, serta larva turunannya (F-2) dengan inseminasi buatan menggunakan sumber spermatofora udang jantan yang berbeda. Perlakuan yang dicobakan adalah induk udang windu betina F-1 diinseminasi menggunakan spermatofora dari sumber induk jantan berbeda yaitu: spermatofora induk jantan F-1 hasil budidaya (S-1), dan spermatofora induk jantan alam (S-A). Data pemijahan induk betina, kualitas telur, dan profil asam amino pada daging dan hepatopankreas jantan, serta morfologi larva dianalisis secara deskriptif, sedangkan data uji vitalitas larva dianalisis uji t. Hasil penelitian menunjukkan bahwa induk udang windu betina F-1 yang diinseminasi dengan perlakuan S-1 memiliki tingkat pemijahan 67%; fekunditas 179.257 butir/induk; total produksi telur 1.434.053 butir; tingkat pembuahan telur 86,2%; daya tetas telur 59,8%; dan total produksi nauplii 738.439 ekor yang lebih rendah dibandingkan pada induk udang F-1 yang diinseminasi perlakuan S-A yang memiliki tingkat pemijahan 75%; fekunditas 215.489 butir/induk; total produksi telur 1.939.399 butir; tingkat pembuahan telur 88,9%; daya tetas telur 62,7%; dan total produksi nauplii 1.081.140 ekor. Sementara diameter telur (248-255 µm) dan mutu larva relatif sama di antara kedua perlakuan. Profil asam amino hepatopankreas dan daging pada induk udang jantan alam lebih tinggi dibandingkan pada induk udang jantan F-1. Penggunaaan spematofora jantan alam masih lebih baik daripada jantan budidaya pada inseminasi induk betina F-1 udang windu.This study was aimed at evaluating the spawning rate of F-1 female tiger shrimp and the quality of their egg and larvae derivatives (F-2) by artificial insemination using different sources of male shrimp spermatophore. The treatments consisted of broodstock of F-1 female tiger shrimp inseminated with different male spermatophores, namely: cultivated F-1 male spermatophore (S-1), and wild male spermatophore (S-A). Data on spawning performance of F-1 female tiger shrimp and amino acid profile in the hepatopancreas and muscle of male tiger shrimp and larval morphology were analyzed descriptively.The vitality of larvae was analyzed using t-test. The results showed that the broodstock of F-1 female tiger shrimp inseminated with S-1 treatment had spawning rate of 67%; fecundity of 179,257 egg; total egg production of 1,434,053 eggs; egg fertilization rate of 86.2%; hatching rate of 59.8%; and total nauplii production of 738,439 ind. Broodstock of F-1 female inseminated with S-A treatment had higher values for spawning rate of 75%, fecundity of 215,489 egg; total egg production of 1,939,399 egg; egg fertilization rate of 88.9%; hatching rate of 62.7%; and total nauplii production of 1,081,140 ind. The produced egg diameter (248-255 ¼m) and larva quality were relatively same between the two treatments. Amino acid profiles in the hepatopancreas and muscle were higher in the wild male broodstock compared to the cultivated (F-1) male broodstock. In conclusion, wild male spermatophore is generally better than the cultivated F-1 male spermatophore for artificial insemination of female broodstock F-1. 

Optimization of Lipase Production from Arthrobacter sp.SD5

2013 ◽  
Vol 791-793 ◽  
pp. 116-119 ◽  
Author(s):  
Peng Ren ◽  
Wei Hua Lin
Keyword(s):  
Carbon Source ◽  
Culture Condition ◽  
Ph Value ◽  
Tween 80 ◽  
Lipase Production ◽  
Medium Composition ◽  
Liquid Volume ◽  
Optimum Conditions ◽  
Culture Temperature ◽  
Initial Ph

Lipase is a kind of important hydrolase. It was widely used in the fields of food, leather, detergent and pharmaceutical. The production of lipase from Arthrobacter sp. SD5 was studied in the present paper. The medium composition and culture condition were optimized in order to improve lipase production. The results showed the best optimum conditions were on the following: culture temperature (40°C); initial pH value (8.0); liquid volume (20%); carbon source (olive oil, 2.5%); nitrogen source (peptone, 1.0%); biosurfactant (Tween-80, 0.2%).

scholarly journals Growth Optimization of Glyphosate-based Herbicides Utilizing Bacteria isolated from Lotic Water of Ogini Stream, Nigeria

2021 ◽  
Vol 2 (6) ◽  
pp. 1-5
Author(s):  
R. E. Aso ◽  
C. Hammuel ◽  
M. Daji ◽  
J. Briska
Keyword(s):  
Bacillus Cereus ◽  
Incubation Time ◽  
Stenotrophomonas Maltophilia ◽  
Incubation Temperature ◽  
High Capacity ◽  
Enterobacter Aerogenes ◽  
Culture Conditions ◽  
Catabolic Genes ◽  
Degrading Bacteria ◽  
Initial Ph

Glyphosate-based herbicides are often used for the control of weeds grown on agricultural fields or farms. Different health problems have been reported to be associated with the use of glyphosate-based herbicides mainly due to their toxicity level. Thus, finding glyphosate utilizing microorganisms to remediate the glyphosate-based herbicides in the environment is crucial. The culture conditions for maximum utilization of glyphosate by bacterial isolates, Stenotrophomonas maltophilia, Bacillus cereus and Enterobacter aerogenes previously isolated from Ugini stream close to corn fields treated with glyphosate-based herbicide at Ofagbe, Delta State, Nigeria were optimized using mineral salt medium containing glyphosate as carbon source. The varied culture parameters assessed were temperature (30, 37 and 40 oC), pH (5, 6, 7, 8 and 9), initial glyphosate concentration (1, 3, 5, 7 and 9 g/L) and incubation time (2-14 days). Optical density (OD) at 560 nm of the culture was used to estimate cell growth or cell load of the glyphosate utilizing bacteria strains at every 2 days for 14 days. The following optimal conditions were determined: initial pH 9.0, incubation temperature 30 °C, initial concentration of glyphosate (1g/L) and incubation time of 12 days. Of the isolates on the medium containing the herbicide as sole carbon and energy source, Bacillus cereus showed the highest growth level (OD average, 0.127, pH average, 6.26. This was followed by Stenotrophomonas maltophilia (OD average = 0.114, pH average = 6.44) and Enterobacter aerogenes (OD average = 0.100, pH average, 6.56). At the increased of glyphosate in the medium there was decreased in growth of the bacteria. Bacillus cereus, Stenotrophomonas maltophilia and Enterobacter aerogenes indicated a high capacity to be able to degrade glyphosate. It is therefore concluded that the bacteria employed in this research can be recommended for bioremediation of environments contaminated with this chemical and further research should conducted to ascertain the catabolic genes present in these individual glyphosate degrading bacteria.

Optimization of Fermentation Medium and Culture Conditions of Hydrocarbon-Degrading Bacterium Brevibacillus panacihumi W25

2014 ◽  
Vol 1010-1012 ◽  
pp. 69-72
Author(s):  
Xin Xin Wang ◽  
Chen Li ◽  
Li Bin Zhao ◽  
Liang Wu ◽  
Ping Zhou ◽  
...  
Keyword(s):  
Contaminated Soils ◽  
Polynomial Regression ◽  
Ph Value ◽  
Fermentation Medium ◽  
Culture Conditions ◽  
Culture Temperature ◽  
Degrading Bacterium ◽  
Initial Ph ◽  
Optimization Of Fermentation ◽  
First Time

Members belonging to the genus Brevibacillus had potentials in the bioremediation of hydrocarbon-contaminated soils. However, little is known about the fermentation medium and culture conditions of Brevibacillus panacihumi. The effect of concentrations of glucose and (NH4)2SO4, initial pH value and culture temperature on the biomass of B. panacihumi W25 was measured with single factor experiments, which was a hydrocarbon-degrading bacterium. Polynomial regression analysis showed optimal concentration of glucose and (NH4)2SO4, initial pH value and culture temperature were 45.2 g/L, 0.6 g/L, pH 7.3 and 30.3°C, respectively. The optimization of fermentation of B. panacihumi was reported for the first time.

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