The Fluorescence In Situ Hybridization Approach Applied in the Study of the Denitrifying Bacteria on Nitration Tank of the Metal Membrane Bioreactor

2011 ◽  
Vol 291-294 ◽  
pp. 2686-2689
Author(s):  
Pan Zhang ◽  
Lei Chen
Keyword(s):  
Membrane Bioreactor ◽  
Denitrifying Bacteria ◽  
Ammonia Nitrogen ◽  
Space Distribution ◽  
Nitrifying Bacteria ◽  
Active Sludge ◽  
Metal Membrane ◽  
The Relationship

To study the amount changes of different denitrifying bacteria in the process of the metal membrane bioreactor, and to confirm the relationship between the amount of different bacteria and the quality of the treating water, the fluorescence in situ hybridization (FISH) dominant molecular biological technologies were applied in this study to preliminary explore the time and space distribution of different denitrifying bacteria populations in the active sludge on the immersed plate-type metal membrane bioreactor. The result indicates that the involvement of the denitrification tank is beneficial to the growth of all bacteria and nitrifying bacteria, meanwhile the eliminate rate of the total nitrogen and ammonia nitrogen is also improved.

The Study of the Fluorescence In Situ Hybridization Applied in the Denitrifying Bacteria on Metal Membrane Bioreactor’s Denitrification Tank

2011 ◽  
Vol 317-319 ◽  
pp. 250-253
Author(s):  
Pan Zhang ◽  
Lei Chen
Keyword(s):  
Membrane Bioreactor ◽  
Removal Rate ◽  
Denitrifying Bacteria ◽  
Ammonia Nitrogen ◽  
Nitrifying Bacteria ◽  
Active Sludge ◽  
Metal Membrane ◽  
The Relationship ◽  
Plate Type

In order to study changes in the quantity of different denitrifying bacteria during the process of the metal membrane bioreactor, and to determine the relationship between different types of bacteria’s quantity and the treating water quality, the fluorescence in situ hybridization (FISH) dominant molecular biological technologies were used in this study to explore different denitrifying bacteria populations in the active sludge in the denitrification tank on the immersed plate-type metal membrane bioreactor. The study result implies that the addition involvement of the denitrification tank is enormous beneficial to the growth of all bacteria and nitrifying bacteria, meanwhile the removal rate of total nitrogen and ammonia nitrogen is improved as well.

The Research of Fluorescence In Situ Hybridization Approach Applied in Denitrification Bacteria in Metal Membrane Bioreactor

2013 ◽  
Vol 864-867 ◽  
pp. 1490-1493
Author(s):  
Pan Zhang ◽  
Yuan Hua Xie
Keyword(s):  
Population Structure ◽  
In Situ Hybridization ◽  
Nitrogen Removal ◽  
Membrane Bioreactor ◽  
Metal Membrane ◽  
Quality Of Water ◽  
Preliminary Study ◽  
Optimized Conditions

To research the composition and proportion of the denitrying bacterium in denitrification tank and increase the nitrogen removals rate. With the purpose of explore the curve between the amount of different denitrying bacterium and the quality of water treating, then find out the optimum of the species of nitrogen removal bacteria and the amount of the specific type of microorganism in the denitrification tank of metal membrane bioreactor at the optimized conditions of the water treating effects. The fluorescence in situ hybridization (FISH) approach is applied in this research, to set a preliminary study on the space-time character of denitrification bacteria population structure in denitrifical tank.

High-strength nitrogen removal of opto-electronic industrial wastewater in membrane bioreactor - a pilot study

2003 ◽  
Vol 48 (1) ◽  
pp. 191-198 ◽  
Author(s):  
T.K. Chen ◽  
C.H. Ni ◽  
J.N. Chen ◽  
J. Lin
Keyword(s):  
Wastewater Treatment ◽  
Industrial Wastewater ◽  
Organic Nitrogen ◽  
Membrane Bioreactor ◽  
High Strength ◽  
Experimental Period ◽  
Ammonia Nitrogen ◽  
Nitrifying Bacteria ◽  
Biological Degradation ◽  
The Past

The membrane bioreactor (MBR) system has become more and more attractive in the field of wastewater treatment. It is particularly attractive in situations where long solids retention times are required, such as nitrifying bacteria, and physical retention critical to achieving more efficiency for biological degradation of pollutant. Although it is a new technology, the MBR process has been applied for industrial wastewater treatment for only the past decade. The opto-electronic industry, developed very fast over the past decade in the world, is high technology manufacturing. The treatment of the opto-electronic industrial wastewater containing a significant quantity of organic nitrogen compounds with a ratio over 95% in organic nitrogen (Org-N) to total nitrogen (T-N) is very difficult to meet the discharge limits. This research is mainly to discuss the treatment capacity of high-strength organic nitrogen wastewater, and to investigate the capabilities of the MBR process. A 5 m3/day capacity of MBR pilot plant consisted of anoxic, aerobic and membrane bioreactor was installed for evaluation. The operation was continued for 150 days. Over the whole experimental period, a satisfactory organic removal performance was achieved. The COD could be removed with an average of over 94.5%. For TOC and BOD5 items, the average removal efficiencies were 96.3 and 97.6%, respectively. The nitrification and denitrification was also successfully achieved. Furthermore, the effluent did not contain any suspended solids. Only a small concentration of ammonia nitrogen was found in the effluent. The stable effluent quality and satisfactory removal performance mentioned above were ensured by the efficient interception performance of the membrane device incorporated within the biological reactor. The MBR system shows promise as a means of treating very high organic nitrogen wastewater without dilution. The effluent of TKN, NOx-N and COD can fall below 20 mg/L, 30 mg/L and 50 mg/L.

Performance and Microbial Characteristics of a Hybrid Biological Reactor Treating Industrial Wastewater

2012 ◽  
Vol 485 ◽  
pp. 438-441
Author(s):  
Chao Li ◽  
Xian Li Li ◽  
Min Ji ◽  
Yu Kun Ma
Keyword(s):  
Microbial Community ◽  
Industrial Wastewater ◽  
Denitrifying Bacteria ◽  
Nitrifying Bacteria ◽  
Cloning And Sequencing ◽  
Attached Growth ◽  
Microbial Characteristics ◽  
Suspended Growth ◽  
Polymerase Chain

To reveal the succession procedure of microbial community in hybrid biological reactor (HBR), the molecular biological techniques of polymerase chain reaction-denaturing gel gradient electrophoresis (PCR-DGGE), cloning and sequencing and fluorescence in situ hybridization (FISH) were applied. PCR-DGGE results showed that the microbial community accumulated in both suspended-growth and attached-growth biomasses. Proteobacteria was found to be the dominant genera of bacteria in the sludge. Denitrifying bacteria was found accumulated in the biofilms. FISH results showed that there were more nitrifying bacteria in the suspended-growth biomass than the attached-growth biomass, but less denitrifying bacteria in the former biomass.

Monitoring of transparent exopolymer particles (TEP) in a membrane bioreactor (MBR) and correlation with other fouling indicators

2008 ◽  
Vol 58 (10) ◽  
pp. 1903-1909 ◽  
Author(s):  
T. de la Torre ◽  
B. Lesjean ◽  
A. Drews ◽  
M. Kraume
Keyword(s):  
Linear Correlation ◽  
Membrane Fouling ◽  
Membrane Bioreactor ◽  
Membrane Systems ◽  
Transparent Exopolymer Particles ◽  
Critical Flux ◽  
Capillary Suction ◽  
Acidic Fraction ◽  
The Relationship

The occurrence of Transparent Exopolymer Particles (TEP), an acidic fraction of polysaccharides, was monitored for more than six months in the activated sludge of three MBR units, and the relationship between TEP and other fouling indicators was studied. These compounds consist mainly of exopolysaccharides of a sticky nature, a characteristic which makes them a group of interesting substances in processes like sedimentation, flocculation and membrane fouling. The relationship between capillary suction time (CST) and polysaccharides (PS) was linear for the three tested sludges, although the correlation with TEP concentrations was stronger. A slight linear correlation of both TEP and PS was found with the critical flux (CF) measured with a small filtration test cell, which was submerged in the membrane tank to assess the filterability performance of the sludge in situ. However, the correlation CF-PS was clearer. The relationship between TEP, polysaccharides and sludge filterability highlights the potential of this parameter for the monitoring of membrane systems.

scholarly journals Morphology of Cleaved Surface and Observation of In Situ Crack Propagation During Cleaving

2021 ◽  
Vol 15 (4) ◽  
pp. 483-491
Author(s):  
Soshi Iwatsuki ◽  
Hirofumi Hidai ◽  
Souta Matsusaka ◽  
Akira Chiba ◽  
Noboru Morita ◽  
...  
Keyword(s):  
Crack Propagation ◽  
High Speed ◽  
Workpiece Material ◽  
Jet Cooling ◽  
Cleavage Line ◽  
The Relationship ◽  
Glass Edge ◽  
Polarization Camera

In laser cleaving, the thermal stress caused by laser heating and water-jet cooling propagates previously induced cracks in the workpiece material. The laser-cleaving conditions affect the quality of the fracture surface, and therefore, elucidating the relationship between the cleaved surface, cleaving conditions, and crack propagation is essential. Against this backdrop, in this study, we investigated the morphology of the cleaved surface and visualized the crack propagation and stress in situ using a high-speed polarization camera. The distance between the glass edge and cleaved surface was varied. When the laser-cleavage line was close to the glass edge, twist hackles were formed on the cleaved surface. The area in which the twist hackles formed on the cleaved surface coincided with the lagging section of the crack front. Furthermore, the twist hackle reached the specimen surface, and the edge of the surface exhibited a sawtooth shape. Observations with the high-speed polarization camera revealed that the internal stress was asymmetric with respect to the crack when the twist hackles were formed.

Application of a membrane bioreactor system for opto-electronic industrial wastewater treatment - a pilot study

2003 ◽  
Vol 48 (8) ◽  
pp. 195-202 ◽  
Author(s):  
T.K. Chen ◽  
J.N. Chen ◽  
C.H. Ni ◽  
G.T. Lin ◽  
C.Y. Chang
Keyword(s):  
Wastewater Treatment ◽  
Industrial Wastewater ◽  
Organic Nitrogen ◽  
Membrane Bioreactor ◽  
Manufacturing Industry ◽  
Ammonia Nitrogen ◽  
Nitrifying Bacteria ◽  
Biological Degradation ◽  
Industrial Wastewater Treatment ◽  
The Past

The membrane bioreactor (MBR) system has become more and more attractive in the field of wastewater treatment. It is particularly attractive in situations where long solids retention times are required, such as nitrifying bacteria, and physical retention is critical to achieving more efficiency for biological degradation of pollutants. Although it is a new technology, the MBR process has been applied to industrial wastewater treatment for only the past decade. The opto-electronic industry, developed very fast over the past decade in the world, is a high technological manufacturing industry. The treatment of the opto-electronic industrial wastewater containing a significant quantity of organic nitrogen compounds, with a ratio over 95% in organic nitrogen (Org-N) to total nitrogen (T-N), is very difficult to meet the discharge limits. The purpose of this research is mainly to discuss the treatment capacity of high-strength organic nitrogen wastewater, and to investigate the capabilities of the MBR process. A 2 m3/day capacity MBR pilot plant consisting of anoxic and aerobic tanks and a membrane bioreactor was installed for evaluation. The operation was continued for 130 days. Over the whole experimental period, a satisfactory organic removal performance was achieved. The COD could be removed with an average of over 94.5%. For TOC and BOD5, the average removal efficiencies were 96.3 and 97.6%, respectively. The nitrification and denitrification were also successfully achieved. The effluent did not contain any suspended solids. Only a small concentration of ammonia nitrogen was found in the effluent. The stable effluent quality and satisfactory removal performance mentioned above were ensured by the efficient interception performance of the membrane device incorporated within the biological reactor. The MBR system shows promise as a means of treating very high organic nitrogen wastewater without dilution. The effluent of TKN, NOx-N and COD can fall below 20 mg/L, 30 mg/L and 50 mg/L.

Activities of Activated Sludge Affected by Pharmaceutical Wastewater in a Membrane Bioreactor

2012 ◽  
Vol 610-613 ◽  
pp. 1426-1431 ◽  
Author(s):  
Yuan Hong Ding ◽  
Qing Wang ◽  
Hong Qiang Ren
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Organic Compounds ◽  
Membrane Bioreactor ◽  
Ammonia Nitrogen ◽  
Nitrifying Bacteria ◽  
Pharmaceutical Wastewater ◽  
Submerged Membrane Bioreactor ◽  
High Concentrations ◽  
Final Effluent

a submerged membrane bioreactor was used to treat the effluent of a pharmaceutical wastewater treatment system, the treated water is rich in ammonia nitrogen and organic compounds (NH4-N, averaged in 78.1 mg/L; COD, averaged in 189.5 mg/L), the final effluent of membrane bioreactor was stably below 50 mg/L COD and 40 mg/L NH4-N respectively, the activity of nitrifying bacteria was inhibited by high concentrations of organic compounds and ammonia nitrogen, a rapid declination of filtration was probably resulted form high concentrations of organic compounds and biomass.

ON THE RELATIONSHIP BETWEEN MATURE STRUCTURE AND ONTOGENY IN THE GRANA OF CHLOROPLASTS

1967 ◽  
Vol 45 (6) ◽  
pp. 773-782 ◽  
Author(s):  
Dominick J. Paolillo Jr. ◽  
Judith A. Reighard
Keyword(s):  
Cyclical Growth ◽  
The Relationship

Some reports on chloroplast infrastructure have emphasized a helical pattern of interconnections among the compartments of the grana. This pattern has been related to a theory of spiral–cyclical growth of grana stacks. The occurrence of multiple helices on the grana in angiosperm chloroplasts impugns the ontogenetic significance of the helical pattern. The compartments of a granum do not discriminate among the helices for quantity or quality of interconnections. No genetic helix can be distinguished. The theory of spiral–cyclical growth of grana, as currently stated, is inadequate to account for the in situ structure of normal grana in the chloroplasts of angiosperms.

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