Composite Cerium Oxide Nanoparticles - Containing Polysaccharide Hydrogel as Effective Agent for Burn Wound Healing

2021 ◽  
Vol 899 ◽  
pp. 493-505
Author(s):  
Anton L. Popov ◽  
Nikolai V. Khohlov ◽  
Nelli R. Popova ◽  
Victoria V. Andreeva ◽  
Kristina A. Kamenskikh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Wound Healing ◽  
Healing Process ◽  
Burn Injuries ◽  
Burn Wound ◽  
Effective Agent ◽  
Physiological Level ◽  
Burn Wounds ◽  
Thermal Burns ◽  
Polysaccharide Hydrogel

A high risk of complications in burn injuries is associated with the development of systemic inflammatory response syndrome. Thermal injury (burn) causes the release of cytokines and prostaglandia, resulting in increased interaction between leukocytes, platelets and endothelial cells. Activation of leukocytes leads to an increase in the formation of reactive oxygen species (ROS) and nitrogen, which is normally compensated by the activation of protective antioxidant enzymes. An imbalance between the activity of the radical-producing and antioxidant systems leads to an excess of free radicals and the development of oxidative stress. Oxidative stress limits the repair of damaged tissue and also leads to localized chronic inflammation. Thus, the removal of inflammation and a decrease in the ROS level, which exceeds the physiological level in the burn zone, seems to be expedient for accelerating the healing process of burn injuries of the skin. In this study, we used a polysaccharide hydrogel modified with cerium dioxide (CeO2) nanoparticles, which have unique anti-inflammatory and antioxidant properties, as an effective agent for the treatment of thermal burns. It has been shown that modification of the hydrogel with CeO2 nanoparticles provides accelerated healing of a model burn wound in rats. Already on the 5th day after the treatment of damage to the skin with the modified hydrogel, a decrease in the area of ​​the burn wound that is different from the control is observed. The use of a hydrogel accelerates the healing process of a burn wound on the 25th day by 25.42% (p <0.05) and ensures complete healing of burn wounds on average 5 days earlier in comparison with the control group with Levomekol ointment. Treatment of burn wounds using a hydrogel leads to the formation of a small post-burn scar. Thus, a polysaccharide hydrogel modified with CeO2 nanoparticles can be considered as an effective wound healing agent in the treatment of thermal burns and skin lesions of various etiologies.

556 The Use of a Concentrated Surfactant Based Gel to Promote Burn Wound Healing

2021 ◽  
Vol 42 (Supplement_1) ◽  
pp. S126-S127
Author(s):  
Rebecca Coffey ◽  
Rachel Penny
Keyword(s):  
Wound Healing ◽  
New Product ◽  
Ease Of Use ◽  
Burn Injuries ◽  
Full Thickness ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Care ◽  
Product Trial ◽  
Burn Wound Healing

Abstract Introduction Strategies to remove necrotic tissue from burn wounds include excisional and non-excisional debridement. Alternative treatments could promote burn wound healing while minimizing patient discomfort and the need for surgery. We evaluated the usage of a concentrated surfactant gel (CSG) to promote burn wound healing in those with indeterminate depth and full thickness burn injuries. Methods An IRB approved retrospective study was conducted during a 10-patient new product trial period with enrollment between September and October 2019. Patients included in this study had indeterminate or full thickness burn wounds and were treated with a concentrated surfactant-based gel. Patients with non-burn diagnoses were excluded. Data collected included demographic information, injury descriptors, and additional burn wound characteristics. Results A total of 10 patients were included in this study as part of a new product trial. The subjects were 80% male with an average TBSA of 7.5%. 40% had indeterminate and 60% had full thickness burn wounds. Prior to initiation of the CSG, the burn wounds had been open for an average of 41 days. There were no infections or complications with usage of the CSG. 90% of patients reported less pain than the standard of care topical agents for burns. Average duration of treatment with the CSG until healing was 28 days. After usage of the CSG, no patients required surgery. Conclusions Our findings support the usage of a concentrated surfactant-based gel in patients with burn wounds. Patients reported decreased pain during dressing changes and ease of use compared to the standard topical agent in burn care. It also prevented surgical debridement in those with indeterminate and full thickness burn injuries.

scholarly journals Sufficient therapeutic effect of cryopreserved frozen adipose-derived regenerative cells on burn wounds

2018 ◽  
Author(s):  
Yasuhiko Kaita ◽  
Takehiko Tarui ◽  
Hideaki Yoshino ◽  
Takeaki Matsuda ◽  
Yoshihiro Yamaguchi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Therapeutic Effect ◽  
Healing Process ◽  
Control Group ◽  
Type I ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Wound Healing ◽  
Significant Difference ◽  
Regenerative Cells

AbstractThe purpose of this study was to evaluate whether cryopreserved (frozen) adipose-derived regenerative cells (ADRCs) have a therapeutic effect on burn wound healing as well as freshly isolated (fresh) ADRCs.Full thickness burns were created on dorsum of nude mice and burn wound was excised. The wound was covered by artificial dermis with; (i) fresh ADRCs, (ii) frozen ADRCs, and (iii) PBS (control). The assessment for wound healing was performed by morphological, histopathological and immunohistochemical analyses.In vivo analyses exhibited the significant therapeutic effect of frozen ADRCs on burn wound healing up to the similar or higher level of fresh ADRCs. There were significant differences of wound closure, epithelized tissue thickness, and neovascularization between the treatment groups and control group. Although there was no significant difference of therapeutic efficacy between fresh ADRC group and frozen ADRC group, frozen ADRCs improved burn wound healing process in dermal regeneration with increased great type I collagen synthesis compared with fresh ADRCs.These findings indicate that frozen ADRCs allow us to apply not only quickly but also for multiple times, and the cryopreserved ADRCs could therefore be useful for the treatment of burn wounds in clinical settings.

scholarly journals A 3D In Vitro Model for Burn Wounds: Monitoring of Regeneration on the Epidermal Level

Biomedicines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1153
Author(s):  
Verena Schneider ◽  
Daniel Kruse ◽  
Ives Bernardelli de Mattos ◽  
Saskia Zöphel ◽  
Kendra-Kathrin Tiltmann ◽  
...  
Keyword(s):  
Wound Healing ◽  
Inflammatory Response ◽  
Healing Process ◽  
Three Dimensional ◽  
Source Model ◽  
Burn Wound ◽  
Thermal Burn ◽  
Burn Wounds

Burns affect millions every year and a model to mimic the pathophysiology of such injuries in detail is required to better understand regeneration. The current gold standard for studying burn wounds are animal models, which are under criticism due to ethical considerations and a limited predictiveness. Here, we present a three-dimensional burn model, based on an open-source model, to monitor wound healing on the epidermal level. Skin equivalents were burned, using a preheated metal cylinder. The healing process was monitored regarding histomorphology, metabolic changes, inflammatory response and reepithelialization for 14 days. During this time, the wound size decreased from 25% to 5% of the model area and the inflammatory response (IL-1β, IL-6 and IL-8) showed a comparable course to wounding and healing in vivo. Additionally, the topical application of 5% dexpanthenol enhanced tissue morphology and the number of proliferative keratinocytes in the newly formed epidermis, but did not influence the overall reepithelialization rate. In summary, the model showed a comparable healing process to in vivo, and thus, offers the opportunity to better understand the physiology of thermal burn wound healing on the keratinocyte level.

scholarly journals The Use of Stem Cells in Burn Wound Healing: A Review

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Fadi Ghieh ◽  
Rosalyn Jurjus ◽  
Amir Ibrahim ◽  
Alice Gerges Geagea ◽  
Hisham Daouk ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Therapeutic Effect ◽  
Healing Time ◽  
Burn Injuries ◽  
Burn Wound ◽  
Burn Wounds ◽  
Complex Processes ◽  
Burn Wound Healing

Burn wound healing involves a series of complex processes which are subject to intensive investigations to improve the outcomes, in particular, the healing time and the quality of the scar. Burn injuries, especially severe ones, are proving to have devastating effects on the affected patients. Stem cells have been recently applied in the field to promote superior healing of the wounds. Not only have stem cells been shown to promote better and faster healing of the burn wounds, but also they have decreased the inflammation levels with less scar progression and fibrosis. This review aims to highlight the beneficial therapeutic effect of stem cells in burn wound healing and to discuss the involved pathways and signaling molecules. The review covers various types of burn wound healing like skin and corneal burns, along with the alternative recent therapies being studied in the field of burn wound healing. The current reflection of the attitudes of people regarding the use of stem cells in burn wound healing is also stated.

scholarly journals Enhancement of Fibroblast Proliferation, Vascularization and Collagen Synthesis in the Healing Process of Third-Degree Burn Wounds by Topical Arnebia euchroma, a Herbal Medicine

2013 ◽  
Vol 1 (2) ◽  
pp. 53-59
Author(s):  
Soheil Ashkani-Esfahani ◽  
Mohammad Hossein Imanieh ◽  
Aidin Meshksar ◽  
Mahsima Khoshneviszadeh ◽  
Ali Noorafshan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Closure ◽  
Healing Process ◽  
Volume Density ◽  
Fibroblast Proliferation ◽  
Burn Wound ◽  
Closure Rate ◽  
Burn Wounds ◽  
Arnebia Euchroma ◽  
Degree Burn

Introduction: The present study was conducted to evaluate the wound healing effect of Arnebia euchroma (AE) extract, which is traditionally used in some Indian, Chinese, and Iranian tribes, on histomorphometrical parameters involved in the healing process of third-degree burn wounds by using stereological analyses. Methods and Materials: In an experimental study, 48 female Sprague-Dawley rats, each with a standard third-degree burn wound on the posterior surface of the neck, were divided into four groups; AE10 and AE20 groups were treated with carboxymethylcellulose (CMC) gels which contained AE hydroalcoholic extract at the concentration of 10% and 20%, respectively; the untreated burned (UB) group, which received no treatment; and the gel-base treated group. Wound closure rate, fibroblast proliferation, volume density of collagen bundles, length density, and mean diameter of the vessels were measured. Results: Wound closure rate, fibroblast population, volume density of collagen bundles, and length density of vessels were significantly improved by AE10 and AE20 in comparison with the gel-base and UB groups (P value <0.05). Conclusion: Although previous investigations on the different aspects of the wound healing effects of AE and the results of this study exhibited the positive effects of topical Arnebia euchroma on third-degree burn wound, introducing AE as an alternative wound healing agent requires more investigations on its efficacy on human, safety, and possible adverse effects.[GMJ. 2012;1(2):53-59]

scholarly journals Topical treatment of standardised burns with herbal remedies in model rats

2005 ◽  
Vol 5 (4) ◽  
pp. 50-57 ◽  
Author(s):  
Fahir Bečić ◽  
Nedžad Mulabegović ◽  
Zakira Mornjaković ◽  
Elvedina Kapić ◽  
Senad Prašović ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Silver Sulfadiazine ◽  
Herbal Remedies ◽  
Control Group ◽  
Therapeutic Effects ◽  
Skin Area ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Wound Healing

Experimental studies of burns require the use of different animal models. The aim of this work was to establish experimental model of thermal injuries and to evaluate the effects of topical agents on healing of the burn wounds. Forty female Wistar rats were randomly classified in 4 groups and isolated for 2 weeks before the onset of experiment. Animals were primarily anaesthetized with pentobarbital-sodium and then shaved (skin area of their back with diameters 5 cm x 5 cm). A round metal stamp with contact area of 5 cm2 and total weight of 100 g was heated up to 80°C and then applied without additional pressure on the depilated skin of the back for 14 seconds. This procedure produced a standardized burn wound. Induced burn wounds were immediately drowned in the 4°C-water for 3 s in order to maintain microcirculation. After the inducement of thermal injures, all rats were treated with 1% silver sulfadiazine cream, herbal topical preparations or were not treated at all. Burn wounds were treated twice a day until the healing completion. The result of treatment application was a significant reduction of burn wound diameters. Herbal topical preparations expressed positive therapeutic effects on the parameters of burn wounds. The efficiency of silver sulfadiazine cream in burn wound healing was significantly more expressed in comparison to healing process in control group of animals (p<0,001). We conclude that herbal topical preparations efficiently caused the completion of burn wound healing process without scar formation.

Topical Insulin Modulates Inflammatory and Proliferative Phases of Burn-Wound Healing in Diabetes-Induced Rats

2019 ◽  
Vol 21 (5) ◽  
pp. 473-484 ◽  
Author(s):  
Flávia Figueiredo Azevedo ◽  
Gabriela Virgínia Moreira ◽  
Caio Jordão Teixeira ◽  
Ana Flávia Marçal Pessoa ◽  
Michele Joana Alves ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Healing Process ◽  
Elastic Fibers ◽  
Enzyme Linked Immunosorbent Assay ◽  
Type I ◽  
Burn Wound ◽  
Burn Wounds ◽  
Inflammatory Phase ◽  
Burn Wound Healing

The healing time of burn wounds depends on surface area and depth of the burn and associated comorbidities. Diabetes mellitus (DM) causes delays in the healing process by extending the inflammatory phase. Treatment with topical insulin can improve the inflammatory phase, restore metabolic dysregulation, and modulate impaired cellular signaling in burn wounds. The objective of this study was to evaluate markers of the inflammatory and proliferative phases of second-degree burns after topical insulin treatment in diabetic rats. Type I DM was induced with streptozotocin in male Wistar rats. The animals’ backs were shaved and subjected to thermal burning. Rats were randomized into two groups: control diabetic (DC) and insulin diabetic (DI). At Days 7 and 14 postburn, rats were euthanized, and wound-tissue sections were evaluated by hematoxylin and eosin, Weigert, and Verhöeff staining, immunohistochemistry-paraffin, and enzyme-linked immunosorbent assay. A significant increase in reepithelialization was seen on Days 7 and 14 in DI versus DC rats. On Day 7, interleukin (IL)-1β, IL-6, monocyte chemotactic protein (MCP)-1, and F4/80 expression were increased in DI versus DC rats. On Day 14, MCP-1 expression was decreased and F4/80 increased in DI versus DC rats. On Days 7 and 14, Ki-67, transforming growth factor-β1, vascular endothelial growth factor expression, and formation of elastic fibers were increased in DI versus DC rats. Topical insulin modulates burn-wound healing in diabetic animals by balancing inflammation and promoting angiogenesis and formation of elastic fibers.

An Overview of the Most Important Medicinal Plants Native to Iran by Property of Burn Wound Healing: A Systematic Review

2017 ◽  
Vol 8 (02) ◽  
Author(s):  
Mehrdad Karimi ◽  
Mahnaz Mardani ◽  
Leila Mahmoodnia
Keyword(s):  
Wound Healing ◽  
Medicinal Plants ◽  
Cinnamic Acid ◽  
Healing Process ◽  
Review Article ◽  
The Body ◽  
Wound Healing Process ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Wound Healing

Burn is an injury where the skin is destroyedby various factors such as heat, cold, electricity, etc.When skin injuryoccurs, pathogens can invade the body and burn wounds get infected shortly after the damage. The wound healing process is a process done by the coordination of tissues, cells and various factors. In the acute phase, inflammatory mechanism of burns has negative effects due to capillary leak. On the other hand, in the later stages of burn,inflammation is necessary for wound healing. Silver nitrate, silver sulfadiazine, and Mafenide acetate are also used for wound care. These compounds cause delayed wound healing and have cytotoxic effects. Iran is among the countries that have rich traditional medicine and herbal treatment of burn wounds in history. Therefore, in this review article, we tried to report the medicinal plants native to Iran which are effective for burn wounds. In this review article, by searching databases such as Scopus, Google Scholar, Spy DVD, mag Iran and… the articles were searched by searching keywords including burn wounds, herbs, extracts, essences and Iran. After finally searching, it was determined that medicinal plants below are the most important medicinal plants native to Iran that affect burn wounds. 11 Medicinal herbs 11 Abukhalsa plant, chamomile, oak, hungry for love, green tea, purslane, cinnamon, Hypericum, and Commiphora are the most important Medicinal Plants which are effective for burn wound healing in Iran. Alkanin, Naphthoquinone and Shikonin, tannins, pectin, alkaloids, saponins and tannins fixed, resin and vitamin C, flavonoids rutin, aucubin and catalpolCatechin, Epicatechin, EpicatechinGallate, Epigallocatechin and Epigallocatechin-gallate, oxalic acid, Cinnamic acid, caffeic acid , maleic acid, Citric acid, coumarin, flavonoids, alanine, tannins, alpha-linolenic acid, cinnamic acid, phenolic compounds such as eugenol, and safrolephellandrene, terpene compounds such as limonene and linalool, trans-cinnamaldehyde (MDA), tannins, coumarin, resin, Cinema-hydroxy phenyl propane compounds such as formaldehyde and mannitol, curcumin, terpenoids, steroids, Flavonoids, lignans are active compounds of plant bioactive substances that are effective on burn wound healing.

scholarly journals Coconut Oil Nanoemulsion Loaded with a Statin Hypolipidemic Drug for Management of Burns: Formulation and In Vivo Evaluation

Pharmaceutics ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1061 ◽  
Author(s):  
Khaled M. Hosny ◽  
Nabil A. Alhakamy ◽  
Amal M. Sindi ◽  
Rasha A. Khallaf
Keyword(s):  
Drug Delivery ◽  
Wound Healing ◽  
Drug Delivery System ◽  
Delivery System ◽  
Healing Process ◽  
Coconut Oil ◽  
Wound Healing Process ◽  
Burn Wound ◽  
Burn Wounds ◽  
The Mean

Burn wound healing is a complex process that involves the repair of injured tissues and the control of infection to diminish the scar formation, pain, and discomfort associated with such injuries. The aim of this research was to formulate and optimize a self-nanoemulsion drug delivery system based on the use of coconut oil and loaded with simvastatin. Coconut oil possesses antiinflammatory and antibacterial activity, and simvastatin has interesting properties for promoting the wound-healing process because it increases the production of the vascular endothelial growth factor at the site of injury. The Box–Behnken design was employed for the optimization of the coconut oil–simvastatin self-nanoemulsion drug delivery system. The prepared formulations were characterized according to globular size and their activity in the healing of burn wounds by assessing the mean wound diameter and level of interlukin-6 in experimental animals. Additionally, the antimicrobial activity of the prepared formulations was assessed. The nanoemulsion was considered adequately formed when it had droplets of between 65 and 195 nm. The statistical design proved the important synergistic effect of coconut oil and simvastatin for burn wound management in their synergistic potentiation of wound closure and their anti-inflammatory and antimicrobial effects. The optimum formulation achieved up to a 5.3-fold decrease in the mean burn wound diameter, a 4.25-fold decrease in interleukin-6 levels, and a 6-fold increase in the inhibition zone against Staphylococcus aureus when compared with different control formulations. Therefore, the designed nanoemulsions containing a combination of coconut oil and simvastatin could be considered promising platforms for the treatment of chronic and burn wounds.

119 A Nitric Oxide Releasing Bio-active Wound Dressing for Burn Wound Infection Treatment

2020 ◽  
Vol 41 (Supplement_1) ◽  
pp. S79-S80
Author(s):  
Jahnabi Roy ◽  
Lori Estes ◽  
Robert J Christy ◽  
Hitesh Handa ◽  
Shanmugasundaram Natesan
Keyword(s):  
Wound Healing ◽  
Wound Infection ◽  
Wound Dressing ◽  
Healing Process ◽  
Fibroblast Proliferation ◽  
Burn Wound ◽  
Burn Wounds ◽  
Log Reduction

Abstract Introduction The future of multi-domain battlefield operation requires wound dressings to prevent infection at the point of injury. Majority of antimicrobial agents only target wound infection while other healing events are left to their natural fates. Nitric oxide (NO) acts against both gram-positive and -negative bacteria and has the potential to positively affect wound healing. In this study we have developed a novel wound dressing integrated with a NO donor - S-nitroso- glutathione (GSNO) in a hybrid formulation of alginate and poly(vinyl alcohol) (PVA) to prevent/treat burn wound infection. Methods The NO releasing wound dressing was fabricated using PVA, alginate, and glycerol, crosslinked with CaCl2 incorporating GSNO. Thereafter, release kinetics were measured up to 4 days. The antibacterial efficacy was determined against both P. aeruginosa and S. aureus. Then the biocompatibility of the NO wound dressing was assessed using in vitro fibroblast proliferation and wound healing assay. Finally, the efficacy of the wound dressing was assessed in vivo using a 3-cm diameter porcine burn wound infection model. Results The Alginate-PVA-GSNO dressing showed a desired physiological level NO flux of 4.42 × 10-10 mol cm-2 min-1 for 72 hours. Alginate−PVA−GSNO dressings showed ~3 log reduction in S. aureus and ~2 log reduction in P. aeruginosa CFU/mg when compared to control. The NO-releasing dressing improved fibroblast proliferation and migration resulting in complete closure of the wound within 48 h in vitro. The safety and efficacy of NO-releasing dressing were successfully established in the both P. aeruginosa and S. aureus infected porcine burn wounds. Histological assessments are carried out to determine the effect of NO-releasing dressing on overall healing process. Conclusions This study shows Alginate−PVA-GSNO wound dressing provides antimicrobial and wound healing properties in vitro. Preliminary in vivo wound healing studies established the safety and efficacy profile of NO-releasing dressing to treat burn wounds. Applicability of Research to Practice An easy to apply, field deployable and effective antimicrobial wound dressing is still a major requisite for combat burn wounds. NO delivering alginate-PVA based wound dressing may be an ideal candidate to inhibit infection as well as promote the wound healing process.

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