Topical treatment of standardised burns with herbal remedies in model rats

Experimental studies of burns require the use of different animal models. The aim of this work was to establish experimental model of thermal injuries and to evaluate the effects of topical agents on healing of the burn wounds. Forty female Wistar rats were randomly classified in 4 groups and isolated for 2 weeks before the onset of experiment. Animals were primarily anaesthetized with pentobarbital-sodium and then shaved (skin area of their back with diameters 5 cm x 5 cm). A round metal stamp with contact area of 5 cm2 and total weight of 100 g was heated up to 80°C and then applied without additional pressure on the depilated skin of the back for 14 seconds. This procedure produced a standardized burn wound. Induced burn wounds were immediately drowned in the 4°C-water for 3 s in order to maintain microcirculation. After the inducement of thermal injures, all rats were treated with 1% silver sulfadiazine cream, herbal topical preparations or were not treated at all. Burn wounds were treated twice a day until the healing completion. The result of treatment application was a significant reduction of burn wound diameters. Herbal topical preparations expressed positive therapeutic effects on the parameters of burn wounds. The efficiency of silver sulfadiazine cream in burn wound healing was significantly more expressed in comparison to healing process in control group of animals (p<0,001). We conclude that herbal topical preparations efficiently caused the completion of burn wound healing process without scar formation.

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Sufficient therapeutic effect of cryopreserved frozen adipose-derived regenerative cells on burn wounds

10.1101/436444 ◽

2018 ◽

Author(s):

Yasuhiko Kaita ◽

Takehiko Tarui ◽

Hideaki Yoshino ◽

Takeaki Matsuda ◽

Yoshihiro Yamaguchi ◽

...

Keyword(s):

Wound Healing ◽

Therapeutic Effect ◽

Healing Process ◽

Control Group ◽

Type I ◽

Burn Wound ◽

Burn Wounds ◽

Burn Wound Healing ◽

Significant Difference ◽

Regenerative Cells

AbstractThe purpose of this study was to evaluate whether cryopreserved (frozen) adipose-derived regenerative cells (ADRCs) have a therapeutic effect on burn wound healing as well as freshly isolated (fresh) ADRCs.Full thickness burns were created on dorsum of nude mice and burn wound was excised. The wound was covered by artificial dermis with; (i) fresh ADRCs, (ii) frozen ADRCs, and (iii) PBS (control). The assessment for wound healing was performed by morphological, histopathological and immunohistochemical analyses.In vivo analyses exhibited the significant therapeutic effect of frozen ADRCs on burn wound healing up to the similar or higher level of fresh ADRCs. There were significant differences of wound closure, epithelized tissue thickness, and neovascularization between the treatment groups and control group. Although there was no significant difference of therapeutic efficacy between fresh ADRC group and frozen ADRC group, frozen ADRCs improved burn wound healing process in dermal regeneration with increased great type I collagen synthesis compared with fresh ADRCs.These findings indicate that frozen ADRCs allow us to apply not only quickly but also for multiple times, and the cryopreserved ADRCs could therefore be useful for the treatment of burn wounds in clinical settings.

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673 Effect of Subcutaneous Topical Ozone Therapy on Second Degree Burn Wounds in Rats: An Experimental Study

Journal of Burn Care & Research ◽

10.1093/jbcr/irab032.319 ◽

2021 ◽

Vol 42 (Supplement_1) ◽

pp. S193-S193

Author(s):

Emre Karakaya ◽

Aydincan Akdur ◽

H Ebru ◽

Ayvazoglu Soy ◽

Alev Ok Atilgan ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

Silver Sulfadiazine ◽

Male Rats ◽

Control Group ◽

Ozone Therapy ◽

Burn Wounds ◽

Degree Burn ◽

Significant Difference ◽

Second Degree

Abstract Introduction Burn is one of the most severe traumas that causes coagulative destruction of the skin. The use of various products that accelerate wound healing in patients with burn may affect the patient’s survival and reduce the complications that may be seen. In the present study we aimed effects of subcutaneous ozone injection on second degree burn wound. Methods A total of 72 Sprague-Dawley male rats included in the study were divided randomly into three groups (control group (CG), silver sulfadiazine group (SG), ozone group (OG)) and each group was divided randomly two subgroups (as sacrificed on d7 and on d14).A deep second degree scald burns were created on the lower back. In CG subcutaneous 0.9% serum saline was injected daily into the burn area. In SG, burns were dressed with silver sulfadiazine daily and in OG subcutaneous ozone was injected daily into the burn area. Tissue hydroxyproline level measurement and histopathological evaluation were done. Results When the groups were compared in terms of weight change, no significant difference was found on the 7th and 14th days. In the evaluation made in terms of tissue hydroxyproline, tissue hydroxyproline level in OG was found to be significantly higher on both the 7th and 14th days (p < 0.001). In histopathological evaluations, it was determined that wound healing in OG was significantly higher than in the other groups. Conclusions According to the results, subcutaneous ozone therapy is more effective than silver sulphadiazine in the healing process of second-degree burn wounds and it can be safely used in the treatment of burn wounds.

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Effects of extractum cepae, heparin, allantoin gel and silver sulfadiazine on burn wound healing: an experimental study in a rat model

Veterinární Medicína ◽

10.17221/6015-vetmed ◽

2012 ◽

Vol 57 (No. 6) ◽

pp. 287-292 ◽

Cited By ~ 7

Author(s):

AS Durmus ◽

M. Yaman ◽

HN Can

Keyword(s):

Wound Healing ◽

Rat Model ◽

Silver Sulfadiazine ◽

Control Group ◽

Albino Rats ◽

Burn Wound ◽

Tissue Samples ◽

Burned Areas ◽

Positive Effects ◽

Burn Wound Healing

These experiments were conducted in order to compare the effects of extractum cepae, heparin, allantoin gel (CTBX) and silver sulfadiazine (SSD) cream on burn wound healing in rats. Thirty six adult, female Wistar albino rats were divided into three equal groups. A burn was made on the back of all rats. The burned areas in the first, second and third groups were covered with cold cream (control), SSD skin cream and CTBX twice a day, respectively. Seven and 14 days later, the rats were sacrificed and burned skin tissue samples were collected from the rats for histopathological examinations. Histopatological evaluations on the 7<sup>th</sup> and 14<sup>th</sup> days showed burn healing to be better in the CTBX and SSD groups with respect to the control group. The best burn wound healing was observed in the CTBX group (P < 0.001). Wound healing was significantly different between the groups at days 7 and 14 (P < 0.001). In conclusion, application of CTBX has significant positive effects on the healing of burn wounds in a rat model.  

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Preparation and evaluation of burns wound healing ointment base of leaves and stem bark of Anthocleista djalonensis (L) extract using animal model.

INTERNATIONAL JOURNAL OF PHARMACEUTICAL EDUCATION AND RESEARCH (IJPER) ◽

10.37021/ijper.v1i2.1 ◽

2020 ◽

Vol 1 (02) ◽

pp. 25-32

Author(s):

Chinenye Nnenna Ugwu ◽

Ezinwanne Nneoma Ezeibe ◽

Chinekwu Sherridan Nwobi ◽

Chinelo Charity Eze ◽

Somtochukwu Amuche Evurani ◽

...

Keyword(s):

Wound Healing ◽

Stem Bark ◽

Positive Control ◽

Silver Sulfadiazine ◽

Wound Contraction ◽

Bark Extract ◽

Control Group ◽

Burn Wound ◽

Burn Wounds ◽

Ointment Base

BACKGROUND: Burn wounds are inescapable life events especially in low income areas. Contamination of the burn wound site results in localized wound infection, hence the need for potent phytochemicals readily available for wound healing. The use of stem bark of Anthocleista djalonensis efficacy for burn wound has not been evaluated to the best of our knowledge. AIM: The study is aimed at evaluating and comparing the wound healing potential of ointment base of leaves and Stem bark of Anthocleista djalonensis on burn wounds created on wistar albino rats. METHODS: The leaves and Stem bark of Anthocleista djalonensis collected were extracted using 95%v/v methanol and phytochemical analysis conducted. Simple ointments of varying concentrations were formulated to screen for wound healing activity using the burn wound model on experimental rats grouped into six (n=4). Group 1 was treated with silver sulfadiazine cream (positive control), group 2 with ointment base (negative control), group 3 with 1% stem bark extract ointment, group 4 with 2% stem bark ointment, group 5 with 1% leave extract ointment, and group 6 with 2% leave extract ointment. All animals were anesthetized before the creation of burn wounds. Measurement was taken on day zero and the wound was left untreated for 48 hours in order to allow bacterial colonization before daily treatment of the wound for 16 days. RESULTS: The result of the phytochemical screening revealed that both extract of Anthocleista djalonensis contains flavonoids, tannins and saponins. On day 2, 1% stem bark, 2% stem bark and 1% leaf extract had 16%, 15%, and 10% wound contraction respectively which was higher than the 8.5% wound contraction of silver sulfadiazine. Also as the concentration of the extract increased, the wound healing effect also increased as seen by the percentage wound contraction on day 16 for all treatment. CONCLUSION: The findings of the study have shown that methanolic extracts of stem bark and leaf of Anthocleista djalonensis contained bioactive constituents which have burn wound healing activity. The stem bark extract showed better activity when compared with the leaf extract and also the positive control (silver sulfadiazine).

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The Effect of Educated Platelets on the Healing Process of Burn Wounds in Rats: Educated Platelets in Rat Burn Wound Healing

Integrative Journal of Medical Sciences ◽

10.15342/ijms.2021.344 ◽

2021 ◽

Vol 8 ◽

Author(s):

Orkun Ilgen ◽

Sefa Kurt ◽

Osman Yilmaz ◽

Murat Celiloglu

Keyword(s):

Wound Healing ◽

Burn Injury ◽

Healing Process ◽

Recovery Period ◽

Control Group ◽

Study Group ◽

Second Line ◽

Burn Wound ◽

Wound Area ◽

Burn Wound Healing

Burns are serious life-threatening health problems. This study was performed to determine the effect of educated platelets on burn wound healing process. 28 female wistar albino 200-220 gr rats were randomly divided into four groups. Group A1 rats(n:7) were the first-line burnt group from which blood samples are extracted to develop platelet-rich plasma(PRP) with the educated platelets that have a response to burn injury. Group B1 rats(n:7) were the unburnt group with ordinary platelets. Group A2 rats(n:8) were the second-line burnt group which was given PRP with educated platelets. Group B2 rats(n:6), as control group, were the second-line burnt group which was given PRP with ordinary platelets. Photos of rats' dorsum were taken by digital camera on the first day and 21st day of the study. Wound healing was determined by scar surface area. In the study group (Group A2) mean wound area was 53±37 mm², in the control group (Group B2) mean wound area was 114±55 mm² on the last day of the experiment. The sizes of the wounded areas were significantly lower in the study group compared with the control group (p: 0.039). Educated platelets seem to facilitate the recovery period of burn wound healing in rats.

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Comparison Profile of Nutrition Status in Stage V Chronic Kidney Disease Patients Hemodialyzing Using New and Reuse Dialyzer

Journal of Agromedicine and Medical Sciences ◽

10.19184/ams.v5i2.9613 ◽

2019 ◽

Vol 5 (2) ◽

pp. 17

Author(s):

Yuli Hermansyah ◽

Ika Aulia Kurniasari ◽

Heni Fatmawati

Keyword(s):

Oxidative Stress ◽

Wound Healing ◽

Antioxidant Properties ◽

Negative Control ◽

Silver Sulfadiazine ◽

Control Group ◽

Burn Wound ◽

Burn Wound Healing ◽

Degree Burn ◽

Significant Difference

Burn wound healing process consists of three phases of inflammation, proliferation and remodelling. The number of fibroblasts has increased during the proliferation phase. Oxidative stress in burn may disrupt proliferation through TGF-β inhibition, result in decreased number of fibroblasts and poor burn wound healing. Genistein in edamame seeds has anti-inflammatory, antibacterial and antioxidant properties. Edamame contains vitamins A, C, and E which is also have antioxidant properties. Edamame has been suggested to protect burn wound healing from oxidative stress. This study aimed to understand the effectiveness of edamame seed (Glycine max L. Merril) ethanolic extract (ED) to fibroblast count in second degree burn wound healing. This is true experimental research with post test only control group design. The sample of this research was 24 rat models of second degree burn which was made by attaching hot metal (60 ° C) for 5 seconds on the shaved back of the rats. The sample was divided into 6 groups with topical treatment, among others: positive control group (silver sulfadiazine), negative control group (Na CMC 0.5%), group with ED 20%, ED 40%, ED 60%, and ED 80%. Treatment was given for 15 days and skin sample was taken on the 16th day. Observation of the number of fibroblasts was done by taking pictures of histhologic preparations in Hematoxylin and Eosin staining under 400x magnification of light microscope. In one way ANOVA test, significance value of 0.011 (p≤0,05) indicated that there was at least a significant difference between 2 groups. LSD’s post hoc analysis showed that there was a significant difference in the group ED 20, 40, and 60% compared with negative control. Keyword: burn wound healing, vegetable soybean, genistein, fibroblast count, silver sulfadiazine

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Evaluation of the Healing Effects of Hypericum perforatum and Curcumin on Burn Wounds in Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/6462956 ◽

2020 ◽

Vol 2020 ◽

pp. 1-5

Author(s):

Nevra Seyhan

Keyword(s):

Wound Healing ◽

Hypericum Perforatum ◽

Control Group ◽

Sprague Dawley ◽

Burn Wound ◽

Burn Wounds ◽

Burn Wound Healing ◽

Significant Difference ◽

Group A ◽

Second Degree

Background. For centuries, medicinal plants have been extensively used in wound healing of burn injuries. The aim of this study is to analyze comparatively the effects of curcumin and Hypericum perforatum (HP) on second-degree burn wounds in rats. Materials and Methods. This experimental study was conducted on 24 male Sprague-Dawley rats with second-degree burns. The animals were randomly divided into three groups. The burns were treated with curcumin (Group B) and Hypericum perforatum (Group C) with daily application. Group A was considered as the control group and received no medication. Histological parameters, reepithelization, granulation tissue formation, inflammation, and angiogenesis were assessed after the scar biopsy at the end of research. Results. All histological parameters of the control group showed statistically significant difference than the other groups (p<0.05). There was no statistically significant difference between Groups B and C in terms of reepithelization and inflammation (p=0.351, p=0.067). In the curcumin group compared with the HP group, a significant difference was observed for two parameters of fibrosis and angiogenesis (p<0.05). Conclusion. Both curcumin and Hypericum perforatum oil are effective in burn wound healing. Our findings showed a better quality of healing in curcumin-treated rats.

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Propolis Modulates Fibronectin Expression in the Matrix of Thermal Injury

BioMed Research International ◽

10.1155/2014/748101 ◽

2014 ◽

Vol 2014 ◽

pp. 1-10 ◽

Cited By ~ 14

Author(s):

Pawel Olczyk ◽

Katarzyna Komosinska-Vassev ◽

Grzegorz Wisowski ◽

Lukasz Mencner ◽

Jerzy Stojko ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

Silver Sulfadiazine ◽

Wound Healing Process ◽

Burn Wound ◽

Burn Treatment ◽

Burn Wound Healing ◽

Fibronectin Expression ◽

The Matrix ◽

Healing Wounds

The aim of the study was to assess the propolis effect on fibronectin metabolism in the course of burn wounds healing process. A model of burn wound healing of pig skin was applied. The amount of the released glycoprotein was assessed by a surface plasmon resonance. The profile of extracted fibronectin components was also assessed by an electrophoresis in polyacrylamide gel, with a subsequent immunodetection by Western Blotting. Propolis burn treatment decreased the release of fibronectin components from healing wounds in relation to damages treated with silver sulfadiazine. The main reason of decreased extraction of fibronectin components from wounds treated with propolis was a substantial decrease of degradation product release of the mentioned glycoprotein, which was observed particularly from the 3rd to 5th day of the repair. Wounds treatment with propolis demonstrated, especially in relation to damages treated with silver sulfadiazine, the decreased release of synthesized fibronectin molecules. The obtained results suggest that propolis modifies fibronectin metabolism in the course of wound healing process. The influence of propolis is reflected in prevention of fibronectin biosynthesis as well as its degradation in the wound area. The above-mentioned metabolic changes may decrease the risk of complications in the repair wounds process.

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Effects of Nigella sativa and silver sulfadiazine on burn wound healing in rats

Veterinární Medicína ◽

10.17221/2948-vetmed ◽

2010 ◽

Vol 55 (No. 12) ◽

pp. 619-624 ◽

Cited By ~ 36

Author(s):

I. Yaman ◽

AS Durmus ◽

S. Ceribasi ◽

M. Yaman

Keyword(s):

Wound Healing ◽

Nigella Sativa ◽

Silver Sulfadiazine ◽

Control Group ◽

Albino Rats ◽

Burn Wound ◽

Tissue Samples ◽

Burned Areas ◽

Burn Wound Healing ◽

Cold Cream

This experiment was conducted in order to compare the effects of Nigella sativa (NS) and silver sulfadiazine (SSD) cream on healing of burn wounds in rats. Fifty four adult, male Wistar-albino rats were divided into three groups of equal numbers. A burn was generated on the backs of all the rats. The burned areas in the first, second and third groups were covered with daily cold cream (control), SSD cream and NS cream (50% NS oil + 50% cold cream), respectively. Four, nine, and 14 days later, the rats were sacrificed and the burned skin tissue samples were collected for histopathological examinations. Histopatological evaluations on the 4th, 9th and 14th days showed burn healing to be better in the NS and SSD groups with respect to the control group. Wound healing was significantly different among the groups at 4th, 9th and 14th days (P < 0.001). In conclusion, application of NS and SSD cream are effective in healing burn related skin wounds in the rat model.

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The Effects of Topical Vitamin C on Burn Wound Healing

Journal of Arak University of Medical Sciences ◽

10.32598/jams.24.2.6140.1 ◽

2021 ◽

Vol 24 (2) ◽

pp. 204-215

Author(s):

Mohammad Haddadi ◽

◽

Davood Movahedzadeh ◽

Elaheh Jaghouri ◽

Hamid Robat Sarpooshi ◽

...

Keyword(s):

Wound Healing ◽

Vitamin C ◽

Topical Application ◽

Repeated Measures ◽

Assessment Tool ◽

Intervention Group ◽

Silver Sulfadiazine ◽

Control Group ◽

Burn Wound ◽

Burn Wounds

Background and Aim: Topical supplements and vitamins have often been used to accelerate the healing of burn wounds. This study investigated the effects of topical application of vitamin C solution on burn wounds. Methods & Materials: This research was performed on 30 patients with extensive second-degree burns on one or two organs with similar burns. The study subjects were randomly divided into the intervention and control groups. The intervention group received topical application of vitamin C solution in addition to silver sulfadiazine ointment in the burn area; however, only silver sulfadiazine ointment was applied in the burn area in the control group. The wounds were examined and scored on days 1, 3, 7, and 14 using the Bates-Jensen Wound Assessment Tool. The collected data were analyzed in SPSS using repeated-measures Analysis of Variance (ANOVA). Ethical Considerations: This study was approved by the ethics committee of sabzevar university medical sciences. (Code: IR.MEDSAB.REC.1394.60). Results Eighteen of the 30 examined patients were male and 12 were female. Their Mean±SD age and body mass index were 43.03±11.90 years and 27.82±4.08 kg/m2, respectively. Conclusion: The repeated-measures ANOVA data indicated that the study groups were significantly different concerning the final mean scores given to the wounds (P=0.047). In other words, the research groups significantly differed in wound healing; thus, topical application of vitamin C significantly improved wound healing in the study participants.

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