Green Analytical Methods in Analysis of Aflatoxins

2016 ◽  
Vol 842 ◽  
pp. 172-181
Author(s):  
Sana Ali
Keyword(s):  
Analytical Methods ◽  
High Performance ◽  
Point Of View ◽  
Free Sample ◽  
Highly Sensitive ◽  
Carcinogenic Effects ◽  
Layer Chromatography ◽  
Nanogram Level ◽  
Animal Consumption

The detection and determination of aflatoxins at nanogram level is important from human health point of view. Aflatoxins exhibit acute toxicity demonstrating teratogenic, mutagenic and carcinogenic effects. Amongst the available aflatoxins, B1, B2, G1, G2, M1 and M2 have been the more frequently analysed aflatoxins by chromatographic, spectroscopic and immuochemical methods. Because of the presence of aflatoxins at trace level in food and feedstuff used for human and animal consumption, new analytical methods have been designed for sensitive and selective detection and quantification of aflatoxins. Each of the employed analytical methods, described herein, such as thin layer chromatography, high performance liquid chromatography, gas chromatography and immunochemical methods, among others, has inherent merits and demerits in aflatoxins analysis. This chapter critically examines each of the analytical methods currently in use in the analysis of aflatoxins. Deviating from the earlier approaches, this chapter focuses on the need of methodologies that have negligible environmental impact. Though most of the existing methods are highly sensitive and reasonably safer to use but still there is the need of development of new green methodologies focusing on the reduction of solvent consumption, replacement of environmentally hazardous solvents with more benign alternatives, miniaturization of instrumentation and solvent-free sample preparation.

Quantitative Determination of Vitamin E Isomers using Gas Chromatography, High Performance Liquid Chromatography and High Performance Thin Layer Chromatography

2017 ◽  
Vol 54 (3) ◽  
pp. 294
Author(s):  
Hari Ramakrishnan K. ◽  
Janaky Ranjithkumar
Keyword(s):  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin E ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Highly Sensitive ◽  
Layer Chromatography

Vitamin E, the fat soluble vitamin is present naturally in some foods and added in food supplements, nutraceuticals etc due to its vital biological function as an antioxidant. Various methods are available for the analysis of vitamin E. Especially High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC) are exclusively used for the quantitative evaluation of vitamin E, which has also identified the four different isomeric forms of this vitamin. The rate of losses of this vitamin during food processing and analysis, in addition to their transient dynamics, presents complexities in developing a highly sensitive procedure for their separations. Though effective, HPLC instrument is expensive and comparatively cumbersome. In this prospective, the study was to evaluate the usefulness of High Performance Thin Layer Chromatography (HPTLC) in the analysis of vitamin E. There are methods available using Thin Layer Chromatography for its analysis, but they are not sensitive enough to identify the isomeric forms of vitamin E. In this HPTLC method, the different isomeric forms of vitamin E - α, β, γ and δ were identified. This technique shall be considered as an alternative to the other methods such as HPLC and GC.

scholarly journals Review for Analytical Methods for the Determination of Mefenamic Acid

2021 ◽  
pp. 426-437
Author(s):  
T. V. Ashira ◽  
T. Mohamed Rafeeque ◽  
Saba Maanvizhi ◽  
S. Kathirvel ◽  
P. C. Indukala ◽  
...  
Keyword(s):  
Mefenamic Acid ◽  
Analytical Methods ◽  
High Performance ◽  
Active Pharmaceutical Ingredient ◽  
Review Article ◽  
Computer Assisted ◽  
Extensive Literature ◽  
Pharmaceutical Dosage Forms ◽  
Layer Chromatography

Mefenamic acid (MFA) is a non-steroidal anti-inflammatory drug that belongs to the anthranilic acid derivative family. It is used to relieve mild to moderate pain. The present review article includes a compilation of articles on the various properties along with an extensive literature survey on the reported analytical methods of MFA. Using a comprehensive computer assisted literature review; this article discusses the analytical methodologies for quantifying MFA both in active pharmaceutical ingredient and pharmaceutical dosage forms. This is the first review article in this series with focus on the analytical profile of MFA. Although, several methods like High Performance Liquid Chromatography (HPLC), Thin Layer Chromatography (TLC), spectrophotometry, fluorimetry, turbidimetry, Atomic Absorption Spectroscopy (AAS), Mass Spectroscopy (MS) and electro analytical methods were reported in the literature, HPLC stands out first for the quantification of MFA.

scholarly journals Rapid, Highly-Sensitive and Ecologically Greener Reversed-Phase/Normal-Phase HPTLC Technique with Univariate Calibration for the Determination of Trans-Resveratrol

Separations ◽  
2021 ◽  
Vol 8 (10) ◽  
pp. 184
Author(s):  
Prawez Alam ◽  
Faiyaz Shakeel ◽  
Mohammed H. Alqarni ◽  
Ahmed I. Foudah ◽  
Mohammed M. Ghoneim ◽  
...  
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Normal Phase ◽  
Commercial Formulation ◽  
Highly Sensitive ◽  
Validation Parameters ◽  
Layer Chromatography ◽  
Chloroform Methanol

The rapid, highly-sensitive and ecologically greener reversed-phase (RP)/normal-phase (NP) high-performance thin-layer chromatography (HPTLC) densitometric technique has been developed and validated for the determination of trans-resveratrol (TRV). The reversed-phase HPTLC-based analysis of TRV was performed using ethanol–water (65:35, v v−1) combination as the greener mobile phase, while, the normal-phase HPTLC-based estimation of TRV was performed using chloroform–methanol (85:15, v v−1) combination as the routine mobile phase. The TRV detection was carried out at 302 nm for RP/NP densitometric assay. The linearity was recorded as 10–1200 and 30–400 ng band−1 for RP and NP HPTLC techniques, respectively. The RP densitometric assay was observed as highly-sensitive, accurate, precise and robust for TRV detection in comparison with the NP densitometric assay. The contents of TRV in commercial formulation were recorded as 101.21% utilizing the RP densitometric assay, while, the contents of TRV in commercial formulation were found to be 91.64% utilizing the NP densitometric assay. The greener profile of RP/NP technique was obtained using the analytical GREEnness (AGREE) approach. The AGREE scales for RP and NP densitometric assays were estimated 0.75 and 0.48, respectively. The recorded AGREE scale for the RP densitometric assay indicated that this technique was highly green/the ecologically greener compared to the NP densitometric assay. After successful optimization of analytical conditions, validation parameters, AGREE scale and chromatography performance, the RP densitometric assay with univariate calibration was found to be better than the NP densitometric assay for the analysis of TRV.

scholarly journals Recent Trends in the Application of Chromatographic Techniques in the Analysis of Luteolin and Its Derivatives

Biomolecules ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 731 ◽  
Author(s):  
Juszczak ◽  
Zovko-Končić ◽  
Tomczyk
Keyword(s):  
Liquid Chromatography ◽  
Medicinal Plants ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Analytical Methods ◽  
High Performance ◽  
Biological Effects ◽  
Immunomodulatory Activity ◽  
Layer Chromatography

Luteolin is a flavonoid often found in various medicinal plants that exhibits multiple biological effects such as antioxidant, anti-inflammatory and immunomodulatory activity. Commercially available medicinal plants and their preparations containing luteolin are often used in the treatment of hypertension, inflammatory diseases, and even cancer. However, to establish the quality of such preparations, appropriate analytical methods should be used. Therefore, the present paper provides the first comprehensive review of the current analytical methods that were developed and validated for the quantitative determination of luteolin and its C- and O-derivatives including orientin, isoorientin, luteolin 7-O-glucoside and others. It provides a systematic overview of chromatographic analytical techniques including thin layer chromatography (TLC), high performance thin layer chromatography (HPTLC), liquid chromatography (LC), high performance liquid chromatography (HPLC), gas chromatography (GC) and counter-current chromatography (CCC), as well as the conditions used in the determination of luteolin and its derivatives in plant material.

scholarly journals Highly Sensitive and Ecologically Sustainable Reversed-Phase HPTLC Method for the Determination of Hydroquinone in Commercial Whitening Creams

Processes ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1631
Author(s):  
Mohammed H. Alqarni ◽  
Prawez Alam ◽  
Faiyaz Shakeel ◽  
Ahmed I. Foudah ◽  
Sultan Alshehri
Keyword(s):  
Analytical Method ◽  
High Performance ◽  
Solvent System ◽  
Reversed Phase ◽  
Highly Sensitive ◽  
Validation Parameters ◽  
Ecologically Sustainable ◽  
Layer Chromatography ◽  
Hptlc Method

Hydroquinone (HDQ) is a natural depigmenting agent, which is commonly used in skin-toning preparations. The safety and greenness of analytical methods of HDQ quantification were not considered in previous literature. Therefore, a highly sensitive and ecologically greener reversed-phase high-performance thin-layer chromatography (RP-HPTLC)-based assay was established for HDQ estimation in four different commercial whitening creams (CWCs). The binary ethanol–water (60:40, v·v−1) mixture was utilized as the green solvent system. The estimation of HDQ was carried out at 291 nm. The present RP-HPTLC-based assay was linear in the 20–2400 ng band−1 range. The present analytical method was highly sensitive based on the detection and quantification data. The other validation parameters, such as accuracy, precision, and robustness, were also suitable for the determination of HDQ. Maximum HDQ quantities were obtained in CWC A (1.23% w·w−1) followed by CWC C (0.81% w·w−1), CWC D (0.43% w·w−1), and CWC B (0.37% w·w−1). The analytical GREEnness (AGREE) score for the present analytical method was estimated as 0.91, indicating the excellent greener characteristics of the present RP-HPTLC assay. These results suggest that the present analytical method is highly sensitive and ecologically sustainable for the quantitation of HDQ in its commercial formulations.

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