The Effect of Mixed Culture of Zymomonas mobilis and Pichia stipitis in Ethanol Production of Sugar Palm (Arenga pinnata)

2019 ◽  
Vol 964 ◽  
pp. 145-150
Author(s):  
Anastasia Sandra Dewi ◽  
Richie Andyllo Stevanus ◽  
Maria Amelia Sandra ◽  
Dennis Farina Nury ◽  
Lily Pudjiastuti ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Mixed Culture ◽  
Fermentation Process ◽  
Ethanol Concentration ◽  
Ethanol Yield ◽  
Tween 80 ◽  
Reducing Sugar ◽  
Organosolv Pretreatment ◽  
Mixed Culture Fermentation ◽  
Single Culture

In this study the effect of mixed culture of Zymomonasmobilis and Pichia stipitis to produce bioethanol from Solid Waste Arenga pinnata (SWAP) was investigated. The fermentation liquid substrate was resulted from an integrated process of pretreatment and enzymatic hydrolysis. Combination of diluted acid and ethanol organosolv pretreatment was used to increase the SWAP lignin removal. Raw pretreatment was used to decrease the SWAP particle size to 200 mesh. Acid pretreatment was done using 5% (v/v) diluted sulfate acid. Acid pretreated SWAP was treated for 65 min with organosolv pretreatment. Enzymatic hydrolysis by a combination of cellulase and xylanase was done for 48 h to convert cellulose into reducing sugar. The surfactants (Tween 80) addition was done to increase the sugar yield of the hydrolysis process. Fermentation variable consist of single culture of Z. mobilis as the control and mixed culture of Z. mobilis and P. stipitis, the microorganisms used to convert glucose and xylose into ethanol. The number of inoculum used in this experiment was more than 1.4 billion cells and the duration of fermentation process was 72 h. The delignification process decreased 95.43% lignin in SWAP. X-ray Diffraction (XRD) analysis assay showed an increase of crystallinity index of SWAP with pretreatment combination to 37.87%. Enzymatic hydrolysis by a combination of cellulase and xylanase with the addition of Tween 80 produced 9.16 gr glucose/L reducing sugar concentration. The highest ethanol resulted by fermentation process using mixed culture of Z. mobilis and P. stipitis with 0.33% (v/v) ethanol concentration and 0.57 (g ethanol/g reducing sugar) ethanol yield. Fermentation process using single culture Z. mobilis resulted 0.28% (v/v) ethanol concentration, and 0.48 (g ethanol/g reducing sugar) ethanol yield. The mixed culture fermentation with Z. mobilis and P. stipitis resulted ethanol yield 19 % higher than the single culture fermentation using Z. mobilis.

Ethanol Production from Non-Detoxified Steam-Exploded Corn Stover Subsequent Enzymatic Hydrolysis by Two Toxin-Tolerant Yeast Strains

2011 ◽  
Vol 365 ◽  
pp. 145-149
Author(s):  
Xun Men ◽  
Xiu Shan Yang ◽  
Shen Tian
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ethanol Production ◽  
Corn Stover ◽  
Fermentation Process ◽  
Ethanol Concentration ◽  
Enzymatic Hydrolysate ◽  
Yeast Strains ◽  
Inhibitory Compounds ◽  
Operation Cost ◽  
Detoxification Process

Fermentation process for ethanol production from steam-exploded corn stover using toxin-tolerant yeast strains was carried out in order to reduce the water consumption and operation cost. The substrate from steam-exploded did not undergo a detoxification process by wash, and was directly hydrolyzed by enzymes. Two toxin-tolerant stains, Y1 and Y5, were tested to ferment the enzymatic hydrolysate slurry directly to ethanol. In the enzymatic hydrolysate slurry containing inhibitory compounds, the strain Y1 and Y5 could convert the sugar to ethanol with ethanol concentration of 47.0 g/L and 47.2 g/L corresponding to 95.9% and 96.4% of the theoretical maximum, respectively.

scholarly journals Bioethanol Production from Enzymatic Hydrolyzates of Pretreated Flaxseed Meals by Baker’s Yeast

2021 ◽  
Author(s):  
SAHELI GHOSAL ◽  
JAYATI BHOWAL
Keyword(s):  
Enzymatic Hydrolysis ◽  
Fermentation Process ◽  
Reducing Sugars ◽  
Reducing Sugar ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Bioethanol Production ◽  
Dilute Acid ◽  
Cellulose Conversion ◽  
Flaxseed Meal

Abstract The present study investigated the usefulness of flaxseed meals as a novel feedstock for the production of bioethanol. The proximate composition of the flaxseed meal was carried out before the pretreatment of the flaxseed meal. In this study, flaxseed meal was pretreated with dilute acid, alkali, and aqueous for disruption of lignocellulosic compounds. The acid pretreated flaxseed meal was used for enzymatic hydrolysis by different enzymes (cellulase, α-amylase, and cellulase combined with α-amylase) for better release of reducing sugar. The cellulose conversion to reducing sugar was significantly higher for acid pretreated flaxseed meals. After enzymatic hydrolysis with cellulase, cellulose conversions to reducing sugars were found to be significantly higher than those of α-amylase and cellulase combined with α-amylase. The bioethanol production was also investigated. The fermentation process was carried out by using baker’s yeast (Saccharomyces cerevisiae) with the acid pretreated flaxseed meal enzymatic hydrolyzate. Maximum ethanol production (0.11 g/l) was achieved from the fermented medium obtained from the acid pretreated flaxseed meal followed by enzymatic hydrolysis by using cellulase enzyme. The structural analysis of bioethanol was also investigated by FTIR.

Effect of Detoxification of Natural Drying Steam Exploded Corn Straw with Organic Solvent on Enzymatic Hydrolysis and Ethanol Fermentation

2012 ◽  
Vol 512-515 ◽  
pp. 379-383
Author(s):  
Xian Chun Jin ◽  
An Dong Song ◽  
Tong Fu Su ◽  
Bia Liang Zhang
Keyword(s):  
Enzymatic Hydrolysis ◽  
Organic Solvent ◽  
Steam Explosion ◽  
Treatment Process ◽  
Ethanol Yield ◽  
Reducing Sugar ◽  
Corn Straw ◽  
Pachysolen Tannophilus ◽  
Fermentation Processes ◽  
Natural Drying

Corn straw from steam-explosion treatment process was treated with different organic solvents to reduce the inhibition for the sequent enzymatic hydrolysis and fermentation. The detoxified substrates (100 g DW/L) were hydrolyzed with a blend of Novozym 188 (15 IU/g cellulose), Celluclast CP cellulase (15 FPU/g cellulose) and lignase (15 IU/g hemicellulose). The reducing sugar was bio-converted to ethanol with separated hydrolysis and fermentation processes using Pachysolen tannophilus P-01. The reducing sugar yield varied from 34.8 to 89.7% depending on the detoxification solvents and modes. The highest ethanol yield (96.1% of theoretical value) was obtained with the combined extraction of ether and acetone.

scholarly journals Production of L-lactic acid from Cassava peel wastes using single and mixed cultures of Rhizopus oligosporus and Lactobacillus plantarum

2014 ◽  
Vol 20 (4) ◽  
pp. 457-461 ◽  
Author(s):  
Ogbonnaya Nwokoro
Keyword(s):  
Lactic Acid ◽  
Mixed Culture ◽  
Lactobacillus Plantarum ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Reducing Sugar ◽  
Rhizopus Oligosporus ◽  
Mixed Culture Fermentation ◽  
Cassava Peels ◽  
Reducing Sugar Concentration

Production of L-lactic acid using cultures of Rhizopus oligosporus and Lactobacillus plantarum was investigated. Cassava peels were hydrolyzed by boiling for 1 h in either NaOH or HCl solutions followed by neutralization to a pH of 6.2. Reducing sugar produced from the hydrolysates increased with increasing concentrations of alkali or acid. Samples hydrolyzed with HCl produced a maximum reducing sugar concentration of 402 mg/g substrate while alkali hydrolyzed samples produced a maximum reducing sugar concentration of 213 mg/g substrate. Hydrolysates were amended with 0.5% ammonium sulphate solution and inoculated with either single or mixed cultures of Rhizopus oligosporus and Lactobacillus plantarum and incubated for 48 h for lactic acid production. The best lactic acid production of 50.2 g/100g substrate was observed in a mixed culture fermentation of acid hydrolyzed peels. Mixed culture fermentation of alkali hydrolyzed peels produced a maximum lactic acid concentration of 36.4 g/100g substrate. Un hydrolyzed cassava peels inoculated with a mixed culture of the microorganisms produced only 4.6 g/100g substrate. This work reports an efficient use of cassava peels for bio-product formation through microbial fermentation.

scholarly journals Influence of 1-butyl-3-methylimidazolium Chloride on the Ethanol Fermentation Process of Pichia pastoris GS115

2015 ◽  
Vol 9 (1) ◽  
pp. 109-112
Author(s):  
Wenjing Huang ◽  
Yanjie Tong ◽  
Wangxiang Huang ◽  
Ke Wang ◽  
Qiming Chen ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Ethanol Fermentation ◽  
Fermentation Process ◽  
Ethanol Concentration ◽  
Ethanol Yield ◽  
Fermentable Sugars ◽  
Yeast Growth ◽  
Ethanol Formation ◽  
Negative Effect ◽  
Final Ethanol Concentration

To evaluate the influence of 1-butyl-3-methylimidazolium chloride ([Bmim]Cl) on the ethanol fermentation process of Pichia pastoris GS115, this paper investigated the yeast growth, ethanol formation and the fermentable sugars consumption during the ethanol fermentation process of Pichia pastoris GS115 at different [Bmim]Cl concentrations in the medium. The results indicated that the [Bmim]Cl had no influence on the ethanol fermentation process at its concentration less than 0.0001 g.L-1. The [Bmim]Cl inhibited the yeast growth and had a negative effect on ethanol formation at its concentration higher than 0.0001 g.L-1. The final biomass and ethanol concentration, and the overall ethanol yield from the fermentable sugars all decreased with its concentration increasing. The yeast growth was very slow and nearly no ethanol formed when its concentration reached 5 g.L-1. Compared to Saccharomyces cerevisiae, the growth of Pichia pastoris GS115 was more sensitive to the [Bmim]Cl, and its ethanol fermentation had lower final ethanol concentration and overall ethanol yield from fermentable sugars at the same [Bmim]Cl concentration. This work provides useful information on selecting suitable strains for ethanol fermentation containing the [Bmim]Cl in the medium.

Enhancement of Enzymatic Hydrolysis of Lignocellulosic Materials by Nonionic Surfactant

2017 ◽  
Vol 757 ◽  
pp. 151-155 ◽  
Author(s):  
Jatuporn Parnthong ◽  
Suratsawadee Kungsanant ◽  
Sumaeth Chavadej
Keyword(s):  
Enzymatic Hydrolysis ◽  
Nonionic Surfactant ◽  
Raw Materials ◽  
Sodium Hydroxide Solution ◽  
Freundlich Isotherm ◽  
Tween 80 ◽  
Reducing Sugar ◽  
Lignocellulosic Materials ◽  
Sugar Production ◽  
Palm Fruit

Palm fruit bunch (PFB) and palm fruit residue (PFR) are considered as potential raw materials for ethanol production due to their large availability from palm oil industry. However, the presence of lignin can retard the rate of enzymatic hydrolysis. Both PFB and PFR were pretreated with a 10% w/v sodium hydroxide solution. The amounts of their hemicellulose were substantially decreased whereas their lignin contents were slightly reduced. After that, they were hydrolyzed using cellulase from Trichoderma reesei (ATCC 26921) at 50 °C and pH 5. An addition of Tween 80, nonionic surfactant, with 0.25% w/v concentration provided an increase in reducing sugar production about 50.5% at 10 FPU/g PFB cellulase loading, while the addition of Tween 80 with 0.5% w/v concentration increased reducing sugar production by 38.8% at 20 FPU/g PFR cellulase loading. The greater the amount of lignin in the lignocellulosic materials, the higher the dosages of enzyme and surfactant required for the enzymatic hydrolysis. The adsorption of added nonionic surfactant onto the hydrophobic surface of lignin resulted in an increase in the availability of added enzyme to both cellulose and hemicellulose, leading to the enhancement of enzymatic hydrolysis. Additionally, the adsorption of cellulase on PFB and PFR were well fitted with the Freundlich isotherm.

scholarly journals Effect of Controlled Microbial Fermentation on Nutritional and Functional Characteristics of Cowpea Bean Flours

Foods ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 530
Author(s):  
Luis Ferreira ◽  
Ana Mendes-Ferreira ◽  
Clícia Benevides ◽  
Diana Melo ◽  
Anabela Costa ◽  
...  
Keyword(s):  
Dietary Fibre ◽  
Fermentation Process ◽  
Titratable Acidity ◽  
Acetic Acid Bacteria ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Soluble Carbohydrate ◽  
Mixed Culture Fermentation ◽  
Single Culture ◽  
Total Dietary Fibre

This study aimed to optimize bean flours fermentation through the use of appropriate technological procedure and, thereby, to obtain a high quality and safe product. In this line, cowpea bean flours with different moisture conditions (10, 20 and 30%) were incubated with (1) a single culture of Lactobacillus plantarum, or (2) a consortium of lactic acid and acetic acid bacteria, together with one strain of Saccharomyces cerevisiae. Effects of inoculation of cowpea beans flours on stability (i.e., evaluated by the decrease in pH), and variations in nutritional characteristics (i.e., protein, starch, water soluble carbohydrates, total dietary fibre) were investigated. In both fermented flours, the effect of fermentation was more noticeable in total water-soluble carbohydrate (WSC) concentration during the fermentation process (P < 0.001), accounted for by metabolic activity of the microorganisms. The pH values progressively decreased (P < 0.001) through the fermentation process, particularly in flours fermented with a single culture of L. plantarum. By contrast, titratable acidity increased (P < 0.001) throughout the fermentation process in F2 and F3, although more noticeable in F3. Total dietary fibre (TDF) was not variable over the time. In relation to the protein content, the fermentations behaved very similarly; F2 had a variation over the time, but the effect was not significant (P = 0.0690). Results revealed small changes in chemical composition except in the case of pH and sugar contents with the values lower in the fermented products (i.e., single- or mixed-culture fermentation), leading to a more stable and safety product. These results indicate that fermented dry beans flours have the potential as functional ingredients for new food formulations.

scholarly journals Evaluation of Some Biochemical Properties of the Juice of Two Varieties of Sweet Potato Grown in the North of Côte d’Ivoire During a Fermentation Process

2020 ◽  
Vol 5 (9) ◽  
pp. 747-753
Author(s):  
MOROH Jean-Luc Aboya ◽  
KOKORA Aya Philomène ◽  
KARAMOKO Detto ◽  
KAMATE Moustapha ◽  
UATTARA Kiyinma ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sweet Potato ◽  
Fermentation Process ◽  
Biochemical Parameter ◽  
Biochemical Properties ◽  
Reducing Sugar ◽  
Cassava Flour ◽  
The North ◽  
Juice Extraction ◽  
Yellow Flesh

Like cassava flour for making “attiéké” (cassava semolina), the sweet potato tuber, after grinding followed by pressing, produces flour and juice. The present work aims to enhance this juice from the sweet potato by a fermentation which leads to an alcoholic drink. The process consists of five steps including juice extraction, gelation, enzymatic hydrolysis by millet malt, pasteurization and fermentation. We have followed the evolution of some chemical and biochemical parameter at each step of the process. The results indicate that enzymatic hydrolysis with millet malt increases the reducing sugar level by 10 times which was 0.074 g/ml ± 0.013 and 0.041 mg/ml ± 0.011 respectively for white-fleshed sweet potato and yellow flesh after extraction. However, the process drops the vitamin C level from 0.1 mg / ml ± 0.013 of white-fleshed potato juice and 0.05 mg / ml ± 0.002 of yellow-fleshed potato juice to 6.6 10-3 mg/ml ± 1.9.10-3 and 5.9.10-3 mg / ml ± 0.8.10-3 in the same order. The optimal production of alcohol during fermentation is obtained with a concentration of 40 mg / L of yeast to reach an alcohol level of 5.56% ± 0.1 and 4.60% ± 0.1 respectively for the potatoes. sweet with yellow flesh and white flesh after 6 hours.

scholarly journals Enhanced co-generation of cellulosic ethanol and methane with the starch/sugar-rich waste mixtures and Tween 80 in fed-batch mode

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Meishan Fan ◽  
Jun Li ◽  
Guican Bi ◽  
Guangying Ye ◽  
Hongdan Zhang ◽  
...  
Keyword(s):  
Cellulosic Ethanol ◽  
Ethanol Concentration ◽  
Ethanol Yield ◽  
Tween 80 ◽  
Fed Batch ◽  
Mixed Fermentation ◽  
Batch Mode ◽  
Mixture Ratio ◽  
Theoretical Yield ◽  
Solids Loading

Abstract Background The mixed-feedstock fermentation is a promising approach to enhancing the co-generation of cellulosic ethanol and methane from sugarcane bagasse (SCB) and molasses. However, the unmatched supply of the SCB and molasses remains a main obstacle built upon binary feedstock. Here, we propose a cellulose–starch–sugar ternary waste combinatory approach to overcome this bottleneck by integrating the starch-rich waste of Dioscorea composita Hemls. extracted residue (DER) in mixed fermentation. Results The substrates of the pretreated SCB, DER and molasses with varying ratios were conducted at a relatively low solids loading of 12%, and the optimal mixture ratio of 1:0.5:0.5 for the pretreated SCB/DER/molasses was determined by evaluating the ethanol concentration and yield. Nevertheless, it was found that the ethanol yield decreased from 79.19 ± 0.20 to 62.31 ± 0.61% when the solids loading increased from 12 to 44% in batch modes, regardless of the fact that the co-fermentation of three-component feedstock was performed under the optimal condition defined above. Hence, different fermentation processes such as fed-batch and fed-batch + Tween 80 were implemented to further improve the ethanol concentration and yield at higher solids loading ranging between 36 and 44%. The highest ethanol concentration of 91.82 ± 0.86 g/L (69.33 ± 0.46% of theoretical yield) was obtained with fed-batch + Tween 80 mode during the simultaneous saccharification and fermentation at a high solids loading of 44%. Moreover, after the ethanol recovery, the remaining stillage was digested for biomethane production and finally yielded 320.72 ± 6.98 mL/g of volatile solids. Conclusions Integrated DER into the combination of SCB and molasses would be beneficial for ethanol production. The co-generation of bioethanol and biomethane by mixed cellulose–starch–sugar waste turns out to be a sustainable solution to improve the overall efficacy in biorefinery.

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