Biosynthesis of Silver Nanoparticles Made from Green Tea Leaf Extract (Camellia sinensis)

2019 ◽  
Vol 967 ◽  
pp. 161-167
Author(s):  
Hasri ◽  
Iwan Dini ◽  
Satria Putra Jaya Negara ◽  
Subaer
Keyword(s):  
Silver Nanoparticles ◽  
Reaction Time ◽  
Green Tea ◽  
Leaf Extract ◽  
Average Diameter ◽  
Particle Size Analyzer ◽  
Green Tea Leaves ◽  
Optimum Reaction ◽  
Tea Leaf

Silver nanoparticles act as anti-bacterial and anti-inflammatory. On the other hand, some plants contain reducing agents. Therefore, it is deemed necessary to know the potentiality of plant extracts such as green tea leaf extract on the synthesis of silver nanoparticles. The Methanol extract of green tea leaves serves as a reduction of AgNO3 solution. Determination of the optimum reaction time in forming nanosize using UV-Vis spectrophotometer every 30 minutes. Characterization of nanoparticles obtained using scanning electron microscopy (SEM) and particle size analyzer (PSA). The results showed that green tea leaf extract was able to reduce Ag + to silver nanoparticles at a reaction time of 90 minutes, the temperature of 70°C. Morphology is not uniform, tends to aggregate, and the size distribution of silver nanoparticles is 82.33-740.89 nm with an average diameter of 157.8 nm.

scholarly journals DEVELOPMENT OF SUNSCREEN PRODUCTS CONTAINING GREEN TEA LEAF EXTRACT

2021 ◽  
pp. 30-33
Author(s):  
SETYO NURWAINI ◽  
LITA ALIANTI ◽  
DHIAH AYU PERMATA SARI ◽  
WAHYU UTAMI
Keyword(s):  
Antioxidant Activity ◽  
Physical Properties ◽  
Green Tea ◽  
Leaf Extract ◽  
Protection Factor ◽  
Green Tea Leaves ◽  
High Antioxidant Activity ◽  
Tea Leaf ◽  
Sunscreen Products

Objective: The purpose of this research was to determine antioxidant activity of green tea leaf extract, the value of sun protection factor (SPF),physical properties, and stability of green tea cream.Methods: Green tea leaf extract was obtained by maceration using 96% ethanol. Cream was prepared in three formulas with various concentrationof the green tea leaves extract. The physical evaluations included organoleptic, pH, viscosity, adhesion, spreadability, and stability tests weredone. The determination of the SPF value is calculated using the Mansur equation. Extract of green tea leaf has strong antioxidant activity(IC50 2.19 μg/ml).Results: The green tea leaf extract showed high antioxidant activity (2.19 μg/m). All formulas are organoleptically creamy brownish-green to brown,with a distinctive green tea odor and homogeneous. All formulas met the requirements of physical properties of cream. The creams showed significantchange while they were stored at 4°C and at 40±2°C, but showed no difference when they were stored ad 26°C. SPF values of cream are 0.54; 2.03,and 2.41, respectively.Conclusions: It is clearly indicated that the sunscreen cream of green tea leaf extract is potential to be further developed as cosmetic preparations.

scholarly journals Sintesis Nanopartikel Perak Menggunakan Bioreduktor Ekstrak Daun Teh Hijau (Camellia Sinensis) dengan Iradiasi Microwave

2020 ◽  
Vol 21 (1) ◽  
pp. 30
Author(s):  
Dewi Mustika Rahim ◽  
Netti Herawati ◽  
Hasri Hasri
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Silver Nanoparticles ◽  
Green Tea ◽  
Leaf Extract ◽  
Synthesis Time ◽  
Particle Size Analyzer ◽  
Average Size ◽  
Tea Leaf ◽  
Scanning Electron

ABSTRAK Telah dilakukan penelitian tentang sintesis nanopartikel perak menggunakan bioreduktor ekstrak daun teh hijau (Camellia Sinensis) dengan iradiasi microwave. yang untuk mengetahui pengaruh waktu sintesis dan pengaruh pH stabilizer terhadap sintesis nanopartikel perak ekstrak daun teh hijau dengan iradiasi microwave serta karakterisasinya. Larutan AgNO3 3 mM direduksi menggunakan ekstrak daun teh hijau dan di iradiasi microwave pada variasi waktu sintesis 1-5 menit. Penentuan waktu sintesis optimal menggunakan spektrofotometer UV-Vis diukur setiap 1 menit. Nanopartikel dengan waktu sistesis 4 menit, pH stabilizer menggunakan asam sitrat dan NaOH dengan pH 6-9. Karakterisasi nanopartikel menggunakan instrumen SEM-EDS (Scanning Electron Microscopy-Energy Dispersive X-Ray Spectroscopy) dan PSA (Particle Size Analyzer). Hasil penelitian menunjukkan bahwa waktu sintesis optimal adalah 4 menit. Morfologi nanopatikel perak yang dikarakterisasi menggunakan SEM (Scanning Electron Microscopy) yang dihasilkan cenderung beragregasi. Adanya kecenderungan nanopartikel untuk beragregasi menyebabkan ukuran atau diameter nanopartikel tidak seragam. Ukuran dan distribusi ukuran nanopartikel perak dikarakterisasi menggunakan PSA(Particle Size Analyzer) dihasilkan pada pH 6 antara 31,01 – 402,44 nm dengan rata-rata ukuran sebesar 91 nm, pH 7 antara 35,03 – 740,899 nm dengan rata-rata ukuran sebesar 106,3 nm, pH 8 antara 39,58 – 193,48 nm dengan rata-rata ukuran 71,7, dan pH 9 antara 35,03 – 171,25 nm dengan rata-rata ukuran sebesar 64,4 nm. Disimpulkan bahwa hasil sintesis nanopartikel perak terkecil diperoleh pada pH 9 dan waktu sintesis 4 menit. Kata kunci: Ekstrak Daun Teh Hijau, Nanopartikel Perak, Iradiasi Microwave ABSTRACT Research has been carried out on the synthesis of silver nanoparticles using a bioreductor of green tea leaf extract (Camellia Sinensis) by irradiation microwave.This research aimed to determine the effect of synthesis time and pH stabilizer on the synthesis of silver nanoparticles of green tea leaf extract by microwave irradiation and it’s characterization. The 3 mM AgNO3 solution was reduced using green tea leaf extract and microwave irradiated in a variation of the synthesis time 1-5 minutes. The determining optimum of sythesis time was done by analysis of UV-Vis spectrum for every minutes. Nanoparticles with a synthesis time of 4 minutes, the stabilizer of silver nanoparticles used citric acid and NaOH with a pH of 6-9. Characterization of nanoparticles using SEM-EDS instrument (Scanning Electron Microscopy-Energy Dispersive X-Ray Spectroscopy) and PSA (Particle Size Analyzer). The results showed that the optimal synthesis time was 4 minutes. The morphology of silver nanopaticles characterized using SEM produced tends to aggregate. The tendency of nanoparticles to aggregate causes size or diameter of nanoparticles was random. The size and distribution of size silver nanoparticles characterized using PSA were produced at pH 6 between 31.01 - 402.44 nm with an average size of 91 nm, pH 7 between 35.03 - 740,899 nm with an average size amounting to 106.3 nm, pH 8 between 39.58 - 193.48 nm with an average size of 71.7, and pH 9 between 35.03 - 171.25 nm with an average size of 64.4 nm. It was concluded that the synthesis of the smallest silver nanoparticles was obtained at pH 9 and synthesis time of 4 minutes. Keywords: Green Tea Leaf Extract, Silver Nanoparticles, Microwave Irradiation

scholarly journals Sintesis Nanopartikel Perak Menggunakan Ekstrak Daun Salam (Syzygium polyanthum) Sebagai Bioreduktor Dan Uji Aktivitasnya Sebagai Antioksidan

2019 ◽  
Vol 7 (1) ◽  
pp. 51-60
Author(s):  
Paulina Taba ◽  
Nadya Yuli Parmitha ◽  
Syahruddin Kasim
Keyword(s):  
Particle Size ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Average Particle Size ◽  
Average Diameter ◽  
Average Particle ◽  
X Ray ◽  
Synthesis Of Nanoparticles ◽  
Particle Size Analyzer ◽  
Ic50 Value

Silver nanoparticles have been synthesized by the reduction method using salam leaf extract (Syzygium polyanthum) as a reducing agent. The silver nanoparticles produced were then tested for their activity as antioxidants. The formation of silver nanoparticles was carried out by adding the leaf extract into the solution of AgNO3 and homogenized using a magnetic stirrer. A UV-Vis spectrophotometer was used to confirm the formation of silver nanoparticles. Particle Size Analyzer (PSA), Scanning Electron Microscope (SEM), X-Ray Diffractometer (XRD) and Fourir Transform Infra Red (FTIR) spectrometers were used to characterize the nanoparticles produced before being tested for antioxidant activity. The results showed that the absorbance value increased with increasing reaction contact time. The maximum uptake was obtained at wavelengths of 432-446 nm using a UV-Vis spectrophotometer. The particle size was determined using a PSA with an average particle size distribution of 45.7 nm. The average diameter of silver nanoparticles was 10.06 – 13.97 nm and the silver nanoparticles had rod-shapes. Functional groups that play a role in the synthesis of nanoparticles were –OH, –C=O, and –C-O groups. Silver nanoparticles inhibited free radicals as antioxidants with the IC50 value of 582.7 ppm.

scholarly journals Development of antibacterial and UV protective cotton fabrics using plant food waste and alien invasive plant extracts as reducing agents for the in-situ synthesis of silver nanoparticles

Cellulose ◽  
2021 ◽  
Author(s):  
Nina Čuk ◽  
Martin Šala ◽  
Marija Gorjanc
Keyword(s):  
Silver Nanoparticles ◽  
Green Tea ◽  
Food Waste ◽  
Cotton Fabrics ◽  
Reducing Agent ◽  
Plant Food ◽  
Japanese Knotweed ◽  
Tea Leaves ◽  
Green Tea Leaves

Abstract The development of cellulose-based textiles that are functionalised with silver nanoparticles (AgNP), synthesised according to a green approach, and offer protection against ultraviolet (UV) radiation and pathogenic bacteria is very important today. In the present work we demonstrate the environmentally friendly approach to obtain such textile material by AgNP synthesis directly (in-situ) on cotton fabrics, using water extracts of plant food waste (green tea leaves, avocado seed and pomegranate peel) and alien invasive plants (Japanese knotweed rhizome, goldenrod flowers and staghorn sumac fruit) as reducing agents. The extracts were analysed for their total content of phenols and flavonoids and their antioxidant activity. The synthesised AgNP on cotton were round, of different size and amount depending on the reducing agent used. The highest amount of AgNP was found for samples where Japanese knotweed rhizome extract was used as reducing agent and the lowest where extracts of goldenrod flowers and green tea leaves were used. Regardless of the reducing agent used to form AgNP, all cotton samples showed excellent protection against E. coli and S. aureus bacteria and against UV radiation with UV protection factor values above 50. The best results for UV protection even after the twelve repetitive washing cycles were found for the sample functionalized with AgNP synthesised with an extract of the Japanese knotweed rhizome. Due to the presence of AgNP on cotton, the air permeability and thermal conductivity decreased. AgNP had no effect on the change in breaking strength or elongation of fabrics. Graphic abstract

scholarly journals Antioxidant Effects of Green Tea Leaf Extract on Chemical properties of Corn Refined Oil of Microwave Fried Potatoes

2019 ◽  
Vol 4 (3) ◽  
pp. 96-101
Author(s):  
Masomeh Hoseini ◽  
Maryam Fahim Danesh
Keyword(s):  
Green Tea ◽  
Leaf Extract ◽  
Polar Compounds ◽  
Butylated Hydroxytoluene ◽  
Heating Process ◽  
Ionic Bond ◽  
Refined Oil ◽  
Peroxide Content ◽  
Tea Leaf ◽  
Fried Potatoes

Introduction: Oxygen, light, heat, metal ions, and enzymes are among the agents that oxidize oils, fats, and high-fat food products which result in oxidative spoilage. Due to the toxicity and cancer risk of oxidative agents, the use of synthetic antioxidants is important for delaying the oxidation processes. The purpose of this study was to evaluate the protective effect of natural antioxidants including green tea leaf extract and butylated hydroxytoluene (BHT) on the oxidation of the corn refined oil of microwave-heated fried potatoes. Methods: To this end, corn refined oil and corn oil were treated with green tea leaf extracts (i.e., 200, 400, 600, and 800 ppm), followed by treating the oils with a combination of green tea leaf extract (600 ppm) and BHT (200 ppm). The samples with no anti-oxidant treatments were used as the controls. Eventually, several parameters related to the corn oils were determined, including peroxide content, acidity, polar compounds, and ionic bond conjugates. Results: Based on the results, significant increases were observed in peroxide, acidity, polar compounds, and ionic bond conjugates in the corn refined oil of microwave fried potatoes (P<0.05). On the other hand, the results showed that the heating process reduced peroxide content (P<0.05). However, acidity increased significantly during the heating process (P>0.05) and the lowest acid value was observed in the mixture of 600 ppm of the green tea leaf with 200 ppm of the BHT. There was no significant difference between the control group and the samples treated with 200 ppm of the green leaf extract (P<0.05) in terms of polar components. In addition, a significant decrease was found in the conjugated dienes by increasing the green tea leaf extract concentration (P<0.05). Finally, the highest protection was achieved in the oils that were treated with a combination of green tea leaf extract and BHT. Conclusion: In general, the green tea leaf extract and BHT have antioxidant activities that make them suitable agents for use in food protection industries.

scholarly journals Determination of chlorothalonil residues in raw tea and green tea leaves by an enzyme-linked immunosorbent assay

2008 ◽  
Vol 33 (4) ◽  
pp. 387-392
Author(s):  
Eriko Hatakeyama ◽  
Hiroko Kajita ◽  
Takashi Sugawara ◽  
Satoru Takahashi
Keyword(s):  
Green Tea ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tea Leaves ◽  
Green Tea Leaves

scholarly journals Effect of feed supplementation with biosynthesized silver nanoparticles using leaf extract of Morus indica L. V1 on Bombyx mori L. (Lepidoptera: Bombycidae)

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Sudip Some ◽  
Onur Bulut ◽  
Kinkar Biswas ◽  
Anoop Kumar ◽  
Anupam Roy ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Bombyx Mori ◽  
Leaf Extract ◽  
Silver Ions ◽  
Average Diameter ◽  
Face Centered Cubic ◽  
Tem Analysis ◽  
Beneficial Effects ◽  
Selected Area Electron Diffraction ◽  
Face Centered

Abstract Herein, we report the synthesis of silver nanoparticles (AgNPs) by a green route using the aqueous leaf extract of Morus indica L. V1. The synthesized AgNPs exhibited maximum UV-Vis absorbance at 460 nm due to surface plasmon resonance. The average diameter (~54 nm) of AgNPs was measured from HR-TEM analysis. EDX spectra also supported the formation of AgNPs, and negative zeta potential value (−14 mV) suggested its stability. Moreover, a shift in the carbonyl stretching (from 1639 cm−1 to 1630 cm−1) was noted in the FT-IR spectra of leaf extract after AgNPs synthesis which confirm the role of natural products present in leaves for the conversion of silver ions to AgNPs. The four bright circular rings (111), (200), (220) and (311) observed in the selected area electron diffraction pattern are the characteristic reflections of face centered cubic crystalline silver. LC-MS/MS study revealed the presence of phytochemicals in the leaf extract which is responsible for the reduction of silver ions. MTT assay was performed to investigate the cytotoxicity of AgNPs against two human cell lines, namely HepG2 and WRL-68. The antibacterial study revealed that MIC value of the synthesized AgNPs was 80 µg/ml against Escherichia coli K12 and Staphylococcus aureus (MTCC 96). Finally, the synthesized AgNPs at 10 µg/ml dosages showed beneficial effects on the survivability, body weights of the Bombyx mori L. larvae, pupae, cocoons and shells weights via enhancing the feed efficacy.

scholarly journals FORMULATION AND DISSOLUTION PROFILE STUDY OF TRANSFERSOME-LOADED MICROSPHERES FROM GREEN TEA LEAF EXTRACT

2018 ◽  
Vol 10 (1) ◽  
pp. 211 ◽  
Author(s):  
Effionora Anwar ◽  
Putri Amalia Handayani
Keyword(s):  
Green Tea ◽  
Leaf Extract ◽  
Spherical Shape ◽  
Entrapment Efficiency ◽  
Polydispersity Index ◽  
Dissolution Profile ◽  
Physicochemical Stability ◽  
Span 80 ◽  
Tea Leaf ◽  
Profile Study

Objective: The aim of this study was to prepare transfersome-loaded microspheres which had good characteristics and physicochemical stability toincrease bioavailability of the polyphenol component of green tea leaf extract in the body.Methods: Transfersomes were prepared using a thin-layer hydration method. Green tea leaf extract transfersomes were formulated in the ratio of95:5, 90:10, and 85:15 based on their phospholipid and Span 80 content.Results: The most successful formula produced transfersomes of a spherical shape, 78.75 nm in size with a polydispersity index of 0.187, zetapotential of −37.5 mV, and entrapment efficiency of 47.96±5.81%. Subsequently, the transfersome was loaded into a microsphere using the spray-drymethod. The microspheres had a non-spherical, wrinkled shape, their size was 2058.44 nm, their polydispersity index was 0.545, their entrapmentefficiency was 59.27±0.59%, their moisture content was 5.21%, and their swelling index was 289.36% after 4 h. The total cumulative amount ofEpigallocatechin-3-gallate after a dissolution test was 69.15±7.66%.Conclusion: The physicochemical stability of transfersome-loaded microspheres was not significantly different from that of transfersome powder.

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