SOME CHARACTERISTICS OF AEROMONAS HYDROPHILA AND VIBRIO SPECIES ISOLATED FROM BACTERIAL DISEASE OUT-BREAKS IN ORNAMENTAL FISH CULTURE IN SRI LANKA

Malaysia is the world's major producer and exporter of ornamental fish, contributing 9% to the global trade and taking the second position after Singapore. Because of their artistic appeal and tremendous commercial value for international trade, ornamental fish recently gain rapid importance for foreign exchange and as a source of employment. While ornamental fish production is growing, there is an increase in infectious diseases, resulting in high fish mortality with significant economic loss. Bacterial disease is a serious problem for ornamental fish industry. Bacterial species surveillance in diseased freshwater ornamental fish from an aquarium shop reveals that Aeromonas hydrophila is the most dominant bacteria isolated. Consequently, Malaysia is stepping up its efforts by implementing the Economic Transformation Program and other biosecurity steps to address the aquaculture issues and encourage the regrowth of the ornamental fish market. Chemotherapeutic medications, phytobiotics, probiotics, yeast extracts, vaccines, and disinfectants can be used in controlling bacteria. Further studies should be done to find new antibacterial agents from natural sources to combat bacterial fish diseases and reduce fish mortality rate in sustainable aquaculture farms. This review summarizes the literature on ornamental fish industries and aquaculture production in relation to A. hydrophila-associated diseases and ornamental fish health management in Malaysia.

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Draft Genome Sequence of Aeromonas hydrophila Strain Ae25, Isolated from a Septicemic Moribund Koi Carp ( Cyprinus carpio ) in Sri Lanka

Genome Announcements ◽

10.1128/genomea.01523-17 ◽

2018 ◽

Vol 6 (5) ◽

Cited By ~ 2

Author(s):

Karim Honein ◽

S. S. S. De S. Jagoda ◽

A. Arulkanthan ◽

Hideki Ushio ◽

Shuichi Asakawa

Keyword(s):

Sri Lanka ◽

Genome Sequence ◽

Cyprinus Carpio ◽

Aeromonas Hydrophila ◽

Draft Genome ◽

Multidrug Resistant ◽

Ornamental Fish ◽

Draft Genome Sequence ◽

Koi Carp ◽

Fish Breeding

ABSTRACT Motile aeromonad septicemia caused by mesophilic strains of Aeromonas hydrophila is a widespread problem in cultured freshwater fish. We announce here the draft genome sequence of the multidrug-resistant A. hydrophila strain Ae25, isolated from a koi carp ( Cyprinus carpio ) with motile aeromonad septicemia that was collected from an ornamental fish-breeding farm in Sri Lanka.

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The effect of vaccination in controling bacterial disease caused by Aeromonas hydrophila in nile tilapia (Oreochromis niloticus)

AQUATIC SCIENCE & MANAGEMENT ◽

10.35800/jasm.0.0.2014.7302 ◽

2014 ◽

pp. 27

Author(s):

Arne A Ratulangi ◽

Reiny Tumbol ◽

Hengky Manoppo ◽

Henneke Pangkey

Keyword(s):

Survival Rate ◽

Aeromonas Hydrophila ◽

Oreochromis Niloticus ◽

Nile Tilapia ◽

Bacterial Disease ◽

Immersion Method ◽

Nile Tilapia Oreochromis Niloticus ◽

Different Ages ◽

Motile Aeromonas Septicaemia

This study aims to apply vaccination against bacterial disease. The purpose of vaccination is to trigger the immune respone both non-specific and specific of fish against bacteria Motile Aeromonas Septicaemia (MAS) caused by Aeromonas hydrophila. The vaccination for fish with different ages: 2-3 weeks and 5 weeks were done using immersion method. The fish were re-vaccinated (booster) after two weeks of the first vaccination. The survival rate was < 50 % for juveniles 2-3 weeks and > 50% for juveniles 5 weeks. Survival rate for juveniles 5 weeks was higher than juveniles of 2-3 weeks. This shows that organs of juveniles of 5 weeks were more complete than the 2-3 weeks juveniles. The age of fish is one of the important factors for successfully vaccination. Penelitian ini bertujuan untuk melakukan penerapan vaksinasi terhadap penyakit bakterial. Vaksinasi ditujukan untuk merangsang respon kekebalan non- spesifik dan spesifik pada tubuh ikan terhadap penyakit Motile Aeromonas Septicaemia (MAS) yang disebabkan oleh bakteri Aeromonas hydrophila. Vaksin diberikan pada benih ikan nila yang berbeda umur yaitu 2-3 minggu dan 5 minggu dengan menggunakan metode perendaman. Ikan divaksinasi ulang (booster) setelah 2 (dua) minggu dari vaksinasi yang pertama. Prosentase kelangsungan hidup < 50 % untuk benih umur 2-3 minggu dan > 50% untuk benih umur 5 minggu. Jumlah kematian benih umur 2-3 minggu lebih tinggi dari 5 minggu. Hal ini menunjukkan bahwa fungsi organ benih umur 5 minggu telah lebih lengkap dari pada benih umur 2-3 minggu. Umur ikan merupakan salah satu faktor penting penentu keberhasilan suatu kegiatan vaksinasi.

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STUDI BACILLUS FIRMUS SEBAGAI KANDIDAT PROBIOTIK DALAM MENGHADAPI Aeromonas hydrophila PADA MEDIA BUDIDAYA The Study of Bacillus firmus as Probiotic Candidate in Supressing Aeromonas hydrophila in Culture Media

SAINTEK PERIKANAN Indonesian Journal of Fisheries Science and Technology ◽

10.14710/ijfst.11.2.111-114 ◽

2016 ◽

Vol 11 (2) ◽

pp. 111

Author(s):

Alfabetian Harjuno Condro Haditomo ◽

Angela Mariana Lusiastuti ◽

Widanarni Widanarni

Keyword(s):

Aeromonas Hydrophila ◽

Pathogenic Bacteria ◽

Culture Media ◽

Inhibition Zone ◽

Probiotic Bacteria ◽

Bacterial Disease ◽

Second Phase ◽

Bacillus Firmus ◽

Culture Methods

ABSTRAK Pengendalian penyakit bakterial yang umum dilakukan dengan pemakaian antibiotik atau bahan kimia sudah tidak diperbolehkan lagi karena menimbulkan patogen yang resisten terhadap bahan kimia tersebut, terlebih jika penggunaan tidak sesuai dengan anjuran yang diberikan. Dampak negatif terhadap kesehatan konsumen berupa residu antibiotik juga menjadi pertimbangan yang harus diperhatikan. Manipulasi terhadap populasi mikroba yang berada di perairan guna pencegahan sebelum terjadinya serangan bakteri yang bersifat mematikan perlu dilakukan sebagaimana konsep probiotik sebagai biokontrol. Tujuan penelitian ini adalah menguji kandidat probiotik dalam menekan atau menghambat bakteri patogen Aeromonas hydrophila. Penelitian ini dilaksananakan dalam dua tahap. Tahap pertama adalah tahap pengujian bakteri kandidat probiotik secara in vitro menggunakan metode zona hambat dan kultur bersama pada media agar. Tahap kedua adalah uji tentang bakteri kandidat probiotik dengan patogen pada media budidaya. Hasil terbaik penelitian tahap pertama pada uji kultur bersama antara kandidat probiotik B. firmus dengan A. hydrophila pada skala in vitro adalah dengan penambahan probiotik B. firmus sebanyak 108 cfu/ml. Sedangkan pada penelitian tahap kedua didapatkan hasil berturut-turut perlakuan D dengan tingkat kelangsungan hidup (SR) mencapai 90%, perlakuan C dengan SR 75%, perlakuan A dengan SR 50% dan perlakuan K dengan SR 50%. Kata kunci: Bacillus firmus, probiotik, Aeromonas hydrophila, media budidaya ABSTRACT Controlling bacterial disease with the use of antibiotics or chemicals is no longer allowed as it results in pathogens that are resistant to the chemicals, especially when not in accordance with the recommendations provided. The negative impactsof the antibiotics residues on the consumers’ health also need to be considered. Manipulation of microbial populations present in the waters as preventation before the lethal attack of bacteria needs to be done which is in accordance with the concept of probiotics as biocontrol.The purpose of this study was to test the probiotic candidates in suppressing or inhibiting pathogenic bacteria Aeromonas hydrophila. This study was conducted in two stages. The first stage was to test a candidate probiotic bacteria in vitro using culture methods and inhibition zone on the media together. The second stage wasto test candidate probiotic bacteria to pathogens on the cultivation media. The best results in the first phase of the research is shared culture test between probiotic candidate B. FIRMUS with A. hydrophila on vitro scale is the addition of the probiotic B. FIRMUS 108 cfu / ml. While in the second phase of the research results obtained successively: treatment D with a survival rate (SR) reaches 90%, treatment C with SR 75%, treatment A with SR 50% and treatment K with SR 50%. Keywords: Bacillus FIRMUS, probiotics, Aeromonas hydrophila, media cultivation

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Application of Costing Techniques in Ornamental Fish Culture

Journal of Scientific Research and Reports ◽

10.9734/jsrr/2017/33739 ◽

2017 ◽

Vol 14 (6) ◽

pp. 1-10

Author(s):

T Umamaheswari ◽

R Jayaraman ◽

N Felix

Keyword(s):

Ornamental Fish ◽

Fish Culture

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The Isolation DNA Chromosomal DNA from Local Isolate of Aeromonas hydrophila Bacteria from East Java

Jurnal Vokasi Indonesia ◽

10.7454/jvi.v6i1.115 ◽

2018 ◽

Vol 6 (1) ◽

Author(s):

Retno Sri Wahjuni ◽

M. Gandul Atik Yuliani

Keyword(s):

Aeromonas Hydrophila ◽

Economic Loss ◽

Dna Purification ◽

Bacterial Disease ◽

Bacterial Cells ◽

Chromosomal Dna ◽

Fish Farms ◽

Antigenic Protein ◽

Ulcer Disease ◽

Improved Sanitation

Aeromoniasis is a bacterial disease caused by Aeromonas hydrophila that affects fish and shrimps in ponds and aquariums. This is an ulcer disease that causes petechiae in scales of fish and may be fatal. It can cause economic loss if it is not treated with medication accompanied with improved sanitation. It affects numerous freshwater fish farms in East Java. A previous study has characterized an antigenic protein derived from the outer membrane protein of A. hydrophila. In order to perform sequencing of the DNA coding for this protein, we first conducted a study for isolating DNA fragments of A. hydrophila via four stages: cultivation and harvesting of bacterial cells, cell lysis, DNA purification, and the concentration of chromosomal DNA. The results will be used as a predictive immunogenic determinant by the method by Kolaskar and Tongaonkar. Keywords: Aeromonas hydrophila, chromosomal DNA, immunogenic determinant

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