Role of Helicobacter pylori specific heat shock protein-60 antibodies in the aetiology of coronary artery disease

The role of chronic infections in causing coronary artery disease (CAD) has been investigated for the past several years. Among them, the role of Helicobacter pylori has stimulated keen interest. Though initial results were conflicting, there are growing data to support the role of H. pylori in CAD. The main mechanism of endothelial damage is hypothesized to be through molecular mimicry involving heat shock proteins. This study was designed to determine the prevalence of H.pylori and cytotoxin associated gene A (cagA) positive H.pylori infection in patients undergoing coronary artery bypass grafting (CABG) and the potential role of anti-H. pylori specific heat shock protein-60 (Hp-HSP-60) antibody response in these patients, for cardiac events. One hundred patients undergoing CABG and 100 controls were studied. The H.pylori infection and cagA status were determined serologically by enzyme-linked immunosorbent assay (ELISA). Hp-HSP-60 Immunoglobulin G (IgG) antibodies were estimated by using an in house ELISA. Although there was no difference in the prevalence of H.pylori infection in patients and controls (74% vs 70%), 58% of patients were infected with cagA positive H.pylori compared to 36% of controls (P=0.002). Mean systemic levels of Hp-HSP-60 IgG were also higher in patients than in controls (27.9 vs 18.7, P=0.0001). These antibody levels were also significantly higher in H.pylori positive patients (P=0.0001). There was a strong correlation between Hp-HSP-60 antibody levels and occurrence of myocardial infarction (P=0.003). CagA positive H.pylori infection may be associated with the development of CAD. High levels of Hp-HSP-60 antibodies may constitute a marker and/or concomitant pathogenic factor of the disease.

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Chlamydia pneumoniae heat shock protein 60 is associated with apoptotic signaling pathway in human atheromatous plaques of coronary artery disease patients

Journal of Cardiology ◽

10.1016/j.jjcc.2011.07.010 ◽

2011 ◽

Vol 58 (3) ◽

pp. 216-225 ◽

Cited By ~ 11

Author(s):

Hem Chandra Jha ◽

Pragya Srivastava ◽

Harsh Vardhan ◽

Laishram Chandreshwor Singh ◽

Apurb Rashmi Bhengraj ◽

...

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Heat Shock ◽

Heat Shock Protein ◽

Signaling Pathway ◽

Chlamydia Pneumoniae ◽

Heat Shock Protein 60 ◽

Apoptotic Signaling ◽

Atheromatous Plaques ◽

Artery Disease

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Antibodies to chlamydial heat shock protein 60 is associated with coronary artery disease but antibodies to human heat shock protein 60 and escherichia coli homologs are not

Journal of the American College of Cardiology ◽

10.1016/s0735-1097(02)81243-8 ◽

2002 ◽

Vol 39 ◽

pp. 277

Author(s):

Olaimatu S.M. Mahdi ◽

Benjamin D. Horne ◽

Kelly Muellen ◽

Joseph B. Muhlestein ◽

Gerald I. Byrne

Keyword(s):

Escherichia Coli ◽

Coronary Artery Disease ◽

Coronary Artery ◽

Heat Shock ◽

Heat Shock Protein ◽

Heat Shock Protein 60 ◽

Artery Disease ◽

Human Heat Shock Protein

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Serum Immunoglobulin G Antibodies to Chlamydial Heat Shock Protein 60 but Not to Human and Bacterial Homologs Are Associated With Coronary Artery Disease

Circulation ◽

10.1161/01.cir.0000031567.10814.d8 ◽

2002 ◽

Vol 106 (13) ◽

pp. 1659-1663 ◽

Cited By ~ 26

Author(s):

Olaimatu S. Mahdi ◽

Benjamin D. Horne ◽

Kelly Mullen ◽

Joseph B. Muhlestein ◽

Gerald I. Byrne

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Heat Shock ◽

Heat Shock Protein ◽

Immunoglobulin G ◽

Serum Immunoglobulin ◽

Heat Shock Protein 60 ◽

Artery Disease

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Antibodies to Human Heat-Shock Protein 60 Are Associated With the Presence and Severity of Coronary Artery Disease

Circulation ◽

10.1161/01.cir.103.8.1071 ◽

2001 ◽

Vol 103 (8) ◽

pp. 1071-1075 ◽

Cited By ~ 136

Author(s):

Jianhui Zhu ◽

Arshed A. Quyyumi ◽

David Rott ◽

Gyorgy Csako ◽

Hongsheng Wu ◽

...

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Heat Shock ◽

Heat Shock Protein ◽

Heat Shock Protein 60 ◽

Artery Disease ◽

Human Heat Shock Protein

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The Role of Heat Shock Protein 60 (HSP60) of Helicobacter pylori in Adhesion of H. pylori to Human Gastric Epithelial Cell

Kansenshogaku zasshi ◽

10.11150/kansenshogakuzasshi1970.72.487 ◽

1998 ◽

Vol 72 (5) ◽

pp. 487-492 ◽

Cited By ~ 2

Author(s):

Hiroyuki YAMAGUCHI ◽

Takako OSAKI ◽

Naoto KURIHARA ◽

Haruhiko TAGUCHI ◽

Shigeru KAMIYA

Keyword(s):

Helicobacter Pylori ◽

Heat Shock ◽

Epithelial Cell ◽

Heat Shock Protein ◽

Gastric Epithelial Cell ◽

Heat Shock Protein 60 ◽

H Pylori

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Association of serum soluble heat shock protein 60 with toll-like receptor 4 polymorphism and severity of coronary artery disease

Heart ◽

10.1136/hrt.2004.059170 ◽

2006 ◽

Vol 92 (5) ◽

pp. 683-685 ◽

Cited By ~ 10

Author(s):

K Mandal

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Heat Shock ◽

Heat Shock Protein ◽

Heat Shock Protein 60 ◽

Toll Like Receptor ◽

Toll Like Receptor 4 ◽

Artery Disease

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Tu-P10:479 Inflammatory markers, heat shock protein 60 and chlamydia pneumoniae infection in patients with stable coronary artery disease treated with PTCA

Atherosclerosis Supplements ◽

10.1016/s1567-5688(06)81180-3 ◽

2006 ◽

Vol 7 (3) ◽

pp. 290

Author(s):

R. Rajtar ◽

W. Kolasinska-Kloch ◽

M. Kloch ◽

B. Kiec-Wilk ◽

L. Partyka ◽

...

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Heat Shock ◽

Heat Shock Protein ◽

Inflammatory Markers ◽

Chlamydia Pneumoniae ◽

Stable Coronary Artery Disease ◽

Heat Shock Protein 60 ◽

Artery Disease

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Serological evidence of association between Helicobacter pylori infection and coronary artery disease

African Journal of Clinical and Experimental Microbiology ◽

10.4314/ajcem.v21i2.2 ◽

2020 ◽

Vol 21 (2) ◽

pp. 88-96

Author(s):

S.M. EL-Ageery ◽

N.S. Gouda ◽

I.M. Fawzy ◽

A. Bahy-Eldeen ◽

R. Mahmoud

Keyword(s):

Coronary Artery Disease ◽

Helicobacter Pylori ◽

Coronary Artery ◽

Density Lipoprotein ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Serological Evidence ◽

Autoimmune Reaction ◽

Artery Disease ◽

H Pylori

Background: Studies have reported relationship between chronic Helicobacter pylori infection and coronary artery disease (CAD). The cytotoxin-associated gene A product (CagA) is an immunodominant protein which indicates infection with virulent H. pylori strains. Significant associations of CagA-positive H. pylori strains with coronary artery disorders have been widely reported. H. pylori is also known to produce different heat shock proteins (HSPs) which can stimulate the production of specific antibody against microbial proteins and capable of eliciting autoimmune reaction against human tissue expressing HSPs such as vascular endothelial cells. The objectives of this study are to investigate the association between H. pylori and CagA with coronary atherosclerosis and CAD, and to determine the possible role of H. pylori HSP60 protein in increasing the risk of CAD development. Methods: This study included 70 patients with stable angina and 70 age and gender-matched controls. Each group was evaluated by clinical history, physical examination, cardiac echocardiography (ECHO) and electrocardiography (ECG) with and without exercise. Fasting blood glucose, total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein (HDL) and triglycerides (TG) were estimated by automated enzymatic methods. H. pylori IgG, CagA IgG and HSP60 IgG were measured by enzyme-linked immunosorbent assay (ELISA) for both groups. Results: The seroprevalence of H. pylori infection was high in both groups; 75.7% in case and 68.6% in control (p=0.346). Serum IgG levels were significantly higher for CagA (p=0.028) and HSP60 (p<0.001) in cases than in controls. There was significant association between H. pylori and CagA IgGs in cases (p=0.007) but no association in controls (p=0.700). Higher HSP60 IgG level was significantly associated with both positive H. pylori IgG (p<0.001) and CagA IgG (p<0.001) in cases but no significant association was found with H. pylori (p=0.815) or CagA (p=0.332) IgG levels in the control group. Serum values were significantly higher for TC (p<0.001), TG (p<0.001) and LDL (p=0.004) while value for HDL was significantly lower (p<0.001) in H. pylori IgG-positive subjects (case and control). Conclusion: There is serological evidence that H. pylori infection may pose a significant risk factor for CAD. Since H. pylori can be eliminated by specific treatment, this may be a good preventive approach for CAD.Key words: H. pylori, coronary artery disease, CagA, HSP60, serology.

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