Serological evidence of association between <i>Helicobacter pylori</i> infection and coronary artery disease

Background: Studies have reported relationship between chronic Helicobacter pylori infection and coronary artery disease (CAD). The cytotoxin-associated gene A product (CagA) is an immunodominant protein which indicates infection with virulent H. pylori strains. Significant associations of CagA-positive H. pylori strains with coronary artery disorders have been widely reported. H. pylori is also known to produce different heat shock proteins (HSPs) which can stimulate the production of specific antibody against microbial proteins and capable of eliciting autoimmune reaction against human tissue expressing HSPs such as vascular endothelial cells. The objectives of this study are to investigate the association between H. pylori and CagA with coronary atherosclerosis and CAD, and to determine the possible role of H. pylori HSP60 protein in increasing the risk of CAD development. Methods: This study included 70 patients with stable angina and 70 age and gender-matched controls. Each group was evaluated by clinical history, physical examination, cardiac echocardiography (ECHO) and electrocardiography (ECG) with and without exercise. Fasting blood glucose, total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein (HDL) and triglycerides (TG) were estimated by automated enzymatic methods. H. pylori IgG, CagA IgG and HSP60 IgG were measured by enzyme-linked immunosorbent assay (ELISA) for both groups. Results: The seroprevalence of H. pylori infection was high in both groups; 75.7% in case and 68.6% in control (p=0.346). Serum IgG levels were significantly higher for CagA (p=0.028) and HSP60 (p<0.001) in cases than in controls. There was significant association between H. pylori and CagA IgGs in cases (p=0.007) but no association in controls (p=0.700). Higher HSP60 IgG level was significantly associated with both positive H. pylori IgG (p<0.001) and CagA IgG (p<0.001) in cases but no significant association was found with H. pylori (p=0.815) or CagA (p=0.332) IgG levels in the control group. Serum values were significantly higher for TC (p<0.001), TG (p<0.001) and LDL (p=0.004) while value for HDL was significantly lower (p<0.001) in H. pylori IgG-positive subjects (case and control). Conclusion: There is serological evidence that H. pylori infection may pose a significant risk factor for CAD. Since H. pylori can be eliminated by specific treatment, this may be a good preventive approach for CAD.Key words: H. pylori, coronary artery disease, CagA, HSP60, serology.

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Cytotoxin-associated gene-A bearing strains of Helicobacter pylori and atrial fibrillation due to ischemic origin: is there a link?

European Journal of Cardiovascular Prevention & Rehabilitation ◽

10.1097/hjr.0b013e328011a2a0 ◽

2007 ◽

Vol 14 (4) ◽

pp. 518-520 ◽

Cited By ~ 9

Author(s):

Hala Mahfouz Badran ◽

Magdi Elsayed Mahfouz

Keyword(s):

Atrial Fibrillation ◽

Coronary Artery Disease ◽

Helicobacter Pylori ◽

Coronary Artery ◽

Enzyme Linked Immunosorbent Assay ◽

C Reactive Protein ◽

Reactive Protein ◽

Atrial Size ◽

Artery Disease ◽

H Pylori

Background Helicobacter pylori CagA strains could increase the risk for atrial fibrillation in patients with coronary artery disease Methods Serological status for H. pylori CagA using enzyme-linked immunosorbent assay, C-reactive protein, total leucocytic count and atrial size were determined in 185 coronary artery disease patients (with and without atrial fibrillation) and 80 healthy subjects (control). Results CagA strain showed a higher prevalence in the atrial fibrillation group. Atrial dimension and C-reactive protein (independent predictors of atrial fibrillation) were significantly increased in the CagA seropositive subgroup Conclus ons There is a strong liaison between H. pylori CagA infection and atrial fibrillation in coronary artery disease. Increased C reactive protein and atrial size in atrial fibrillation patients may reflect atrial inflammatory remodeling.

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Association of C1q/TNF-related protein-1 (CTRP1) serum levels with coronary artery disease

Journal of International Medical Research ◽

10.1177/0300060519847372 ◽

2019 ◽

Vol 47 (6) ◽

pp. 2571-2579 ◽

Cited By ~ 9

Author(s):

Linhui Shen ◽

Shuhong Wang ◽

Yuan Ling ◽

Wei Liang

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Tumor Necrosis Factor ◽

Tumor Necrosis ◽

Multiple Logistic Regression Analysis ◽

Serum Levels ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Artery Disease ◽

Necrosis Factor

Objective Complement C1q tumor necrosis factor-related proteins (CTRPs), belonging to the CTRP superfamily, are extensively involved in regulating metabolism and the immune-inflammatory response. The inflammatory process is linked to the pathogenesis of coronary artery disease (CAD). Here, we investigated the association of serum levels of CTRP1 with CAD. Methods Study participants were divided into two groups according to the results of coronary angiography: a control group (n = 63) and a CAD group (n = 76). The concentrations of serum CTRP1 and inflammatory cytokines were determined by enzyme-linked immunosorbent assay. Further analysis of CTRP1 levels in individuals with different severities of CAD was conducted. The CAD severity was assessed by Gensini score. Results Serum levels of CTRP1 were significantly higher in CAD patients than in controls (17.24 ± 1.07 versus 9.31 ± 0.56 ng/mL), and CTRP1 levels increased with increasing severity of CAD. CTRP1 levels were positively correlated with concentrations of tumor necrosis factor-α and interleukin-6. Multiple logistic regression analysis showed that CTRP1 was significantly associated with CAD. Conclusions Our data showed close associations of serum CTRP1 levels with the prevalence and severity of CAD, indicating that CTRP1 can be regarded as a novel and valuable biomarker for CAD.

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Coronary artery disease risk predicted by plasma concentrations of high-density lipoprotein cholesterol, apolipoprotein AI, apolipoprotein B, and lipoprotein(a) in a general Chinese population

Clinical Chemistry ◽

10.1093/clinchem/39.2.209 ◽

1993 ◽

Vol 39 (2) ◽

pp. 209-212 ◽

Cited By ~ 18

Author(s):

J H Wu ◽

J T Kao ◽

M S Wen ◽

D Wu

Keyword(s):

Risk Factors ◽

Coronary Artery Disease ◽

Coronary Artery ◽

High Density Lipoprotein ◽

High Density Lipoprotein Cholesterol ◽

Apolipoprotein B ◽

Density Lipoprotein ◽

Control Group ◽

Apolipoprotein Ai ◽

Artery Disease

Abstract We measured lipid and lipoprotein concentrations in blood samples from control subjects and patients with coronary artery disease (CAD) in Taiwan. We found significant differences (P < 0.01) in the concentrations of high-density lipoprotein cholesterol (HDLC), apolipoprotein AI (ApoAI), apolipoprotein B (ApoB), and lipoprotein(a) [Lp(a)]. Concentrations of HDLC < 350 mg/L, ApoAI < 900 mg/L, ApoB > 800 mg/L, and Lp(a) > 200 mg/L occurred, respectively, 2.8, 5.2, 1.7, and 2.3 times more frequently in the patients than in the control group. If one considers HDLC at < 350 mg/L, ApoAI at < 900 mg/L, ApoB at > 800 mg/L, and Lp(a) at > 200 mg/L as separate risk factors for CAD, the ratio of individual patients to control subjects having 4, 3, 2, 1, or 0 risk factors was [symbol: see text] 9.4, 2.1, 0.2, 0.2, respectively. Individuals displaying three or more risk factors were found 15 times more frequently in the CAD group than in the control group. These risk factors may be used clinically for the prediction and prevention of CAD in the general population.

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Reduced Paraoxonase 1 Activity as a Marker for Severe Coronary Artery Disease

Disease Markers ◽

10.1155/2013/816189 ◽

2013 ◽

Vol 35 ◽

pp. 97-103 ◽

Cited By ~ 21

Author(s):

Chiyan Zhou ◽

Jia Cao ◽

Liang Shang ◽

Chuanfeng Tong ◽

Hanling Hu ◽

...

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Atherosclerotic Lesion ◽

Density Lipoprotein ◽

Hdl Cholesterol ◽

Paraoxonase 1 ◽

Pon1 Activity ◽

Control Group ◽

Potential Biomarker ◽

Artery Disease

Paraoxonase-1 (PON1), a high-density-lipoprotein- (HDL-) associated enzyme, has the potential to protect against atherogenesis. We examine the relationships between plasma PON1 activity and the progression of atherosclerosis as well as coronary artery disease (CAD). Fasting blood samples were collected from female apolipoprotein E-deficient (apoE−/−) mice and 149 patients undergoing coronary angiography for the biochemical parameters measurement. The severity of CAD was defined using angiographic Gensini score (GSS). Compared to 3-month-old apoE−/−mice, aged mice had significantly lower PON1 activity, which is negatively correlated with the size of atherosclerotic lesion and plasma interleukin-6 (IL-6) and tumor necrosis factorα(TNF-α) levels. In study patients, PON1 activity was correlated with age, sex, and HDL-cholesterol, apolipoprotein AI, and high-sensitivity C-reactive protein (hs-CRP) levels and was significantly lower in CAD group than that in non-CAD control group. Interestingly, PON1 activity in severe CAD group (GSS > 40) was further significantly reduced compared to those in mild and moderate subgroups (GSS ≤ 40) (P<0.01). There is a significant correlation between PON1 activity and the severity of CAD as assessed by GSS (r=-0.393,P<0.001). PON1 activity may be a potential biomarker for the severity of CAD.

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ID: 35: ASSOCIATION BETWEEN HELICOBACTER PYLORI INFECTION WITH CORONARY ARTERY DISEASE AND ACUTE MYOCARDIAL INFARCTION: A RETROSPECTIVE CHART ANALYSIS

Journal of Investigative Medicine ◽

10.1136/jim-2016-000120.58 ◽

2016 ◽

Vol 64 (4) ◽

pp. 940-941

Author(s):

N Vyas ◽

H Alkhawam ◽

E Saker ◽

R Sogomonian ◽

RA Ching Companioni ◽

...

Keyword(s):

Myocardial Infarction ◽

Coronary Artery Disease ◽

Acute Myocardial Infarction ◽

Helicobacter Pylori ◽

Risk Factor ◽

Coronary Artery ◽

Medical Center ◽

Control Group ◽

Igg Antibody ◽

Artery Disease

IntroductionHelicobacter pylori (HP) infection is known to target the gastrointestinal system and is associated with extra gastrointestinal manifestations, but there is limited literature on cardiac associations. The most supported pathogenesis uses chronic inflammation as a risk factor causing atherosclerosis resulting in cardiovascular disease. Our aim is to evaluate whether there is an association between HP infection and acute myocardial infarction (AMI) and coronary artery disease (CAD).MethodWe performed a retrospective single center study at our medical center from 2005 to 2014 consisting of 1,671 patients who underwent Coronary Angiography (CA). We divided these patients into two groups based on CA reports. Patients with CAD defined as left main stenosis of ≥50% or any stenosis of ≥70% versus normal coronaries. We reviewed each patient chart to determine the prevalence of positive serum HP IgG antibody. Smoking, hypertension, dyslipidemia and obesity were also considered in each group.ResultsOf 1,671 patients, 1,237 had evidence of CAD vs 434 with normal coronary arteries. Twelve percent of CAD patients were found to have seropositive HP (SPHP) versus 1% in the control group (OR: 7.3, 95% CI: 3.5–15, p<0.0001) as depicted in figure 1. When we looked at the CAD group and compared SPHP patients to seronegative HP (SNHP) patients we found a greater amount of multiple coronary vessels disease in the SPHP group (OR: 1.4, 95% CI: 1.1–2, P=0.04). With regards to AMI, 30% of the SPHP group presented with AMI versus 10% seen in the SNHP group (OR: 4.3, 95% CI: 3–6.5, p<0.0001). In the CAD group with SPHP there was more hyperlipidemia and a higher BMI than in the CAD SNHP group (p<0.0001 and <0.0001, respectively), but there was no statistical difference between the two groups for the risk factors of smoking, hypertension and diabetes.ConclusionAccording to this study, the results showed a correlation with SPHP patients and CAD. Patients with HP seropositivity also tend to have multiple coronary artery vessel disease. In addition, our results also confirmed that there is an association between with HP infection and AMI. We hypothesize that the associated maybe secondary to inflammatory reaction associated with HP. Additional studies with larger sample groups are needed to investigate the possible role of this pathogen as a risk factor for heart disease.Abstract ID: 35 Figure 1Twelve percent of CAD patients were found to have seropositive HP (SPHP) versus 1% in the control group (OR: 7.3, 95% CI: 3.5–15, p<0.0001).

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Positive Correlation between Circulating Fetuin A and Severity of Coronary Artery Disease in Military Males

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1871530320666200601164253 ◽

2020 ◽

Vol 20 ◽

Cited By ~ 1

Author(s):

Reza Afrisham ◽

Maliheh Paknejad ◽

Davod Ilbeigi ◽

Sahar Sadegh-Nejadi ◽

Sattar Gorgani-Firuzjaee ◽

...

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Military Personnel ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Ectopic Calcification ◽

Dual Function ◽

Fetuin A ◽

Nonalcoholic Fatty Liver ◽

Artery Disease

Introduction: Fetuin-A serves a dual function; its high levels are associated with metabolic syndrome, type 2 diabetes, obesity, insulin resistance, and nonalcoholic fatty liver disease and on the other hand, it serves as a potent inhibitor of vascular ectopic calcification. Because of the opposing findings, the aim of the current study was to investigate serum fetuin-A levels in military personnel males with coronary artery disease (CAD). Methods: In the case-control study, anthropometric and biochemical parameters were determined in 83 military personnel males (43 CAD patients and 40 control subjects). At last, the serum fetuin-A levels were measured using the fetuin-A human enzyme-linked immunosorbent assay (ELISA) kit. Results: A significant differences were detected among the two groups for triglyceride and cholesterol levels (P=0.003 and P=0.002, respectively). The mean fetuin A levels were determined 230.57 ± 63.76 and 286.35 ± 64.07 µg/ml for the control group and the CAD patients, respectively (P<0.001). Fetuin A were significantly correlated to the severity of CAD (r 0.393, P<0.001) and associated with the risk of CAD in subjects (OR [CI] = 1. 144 [1.060–1. 235]; p = 0.001). A cut-off value of 237.4 µg/ml had good sensitivity (76.7%) and specificity (65.0%) for differentiating between two groups [area under curve (AUC) = 0.732 (CI=0.621–0.842); p < 0.001]. Conclusion: Our results indicated that fetuin A levels were positively correlated to the severity of CAD. The findings suggest that there are a possible link between pathogenic mechanisms of atherosclerosis and fetuin A; however, more investigations are needed in this regard.

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Abstract 387: HDL Structure and Function in Individuals with Extreme High HDL-C Who Have Coronary Artery Disease Compared with Those Without CAD

Arteriosclerosis Thrombosis and Vascular Biology ◽

10.1161/atvb.33.suppl_1.a387 ◽

2013 ◽

Vol 33 (suppl_1) ◽

Author(s):

Anandita P Agarwala ◽

Jeffrey Billheimer ◽

Amrith Rodrigues ◽

Marjorie Risman ◽

Marina Cuchel ◽

...

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Cholesterol Acyltransferase ◽

Density Lipoprotein ◽

Study Groups ◽

Epidemiologic Studies ◽

Control Group ◽

Older Individuals ◽

Premature Cad ◽

Artery Disease

Plasma levels of high-density lipoprotein cholesterol (HDL-C) are strongly inversely associated with coronary artery disease (CAD) in epidemiologic studies, and high HDL-C is generally associated with apparent ‘protection’ from CAD. We have been recruiting individuals with high HDL-C for about 15 years, and while most have no CAD, a minority has premature CAD, a paradoxical phenotype. We hypothesize that such individuals may have HDL with altered structure and/ or function, and are systematically comparing these individuals (cases) to older individuals with extreme high HDL-C without CAD (controls) and a healthy control group with normal HDL-C levels. We identified 60 subjects with HDL-C above the 90th percentile, premature CAD, and no other major risk factors for coronary disease. We selected 2 controls per case, each matched for age, race, gender, and HDL level. Demographic information and lipid profile (mean ± SD) of the study groups are shown below. Controls are well matched to the cases. Studies are well underway to assess HDL size distribution by NMR, HDL composition, total and ABCA1-specific cholesterol efflux capacity, lecithin-cholesterol acyltransferase (LCAT) activity, and cholesteryl ester transfer protein (CETP) activity in cases and controls. We will also compare to a group of healthy controls with normal HDL-C levels. We expect that the findings from this study will provide insight into the etiology of CAD in this paradoxical phenotype.

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Association between Helicobacter. pylori and coronary artery disease

Open Medicine ◽

10.2478/s11536-010-0051-4 ◽

2011 ◽

Vol 6 (1) ◽

pp. 107-112

Author(s):

Setareh Davoudi ◽

Abbas Omran ◽

Mohammad Boroumand ◽

Nasrin Rahimian ◽

Soheil Saadat

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Predictive Factor ◽

Coronary Atherosclerosis ◽

Multivariable Analysis ◽

Density Lipoprotein ◽

Control Group ◽

Independent Predictive Factor ◽

Significant Difference ◽

Artery Disease

AbstractThe high prevalence of both Helicobacter.pylori infection and coronary atherosclerosis in our country prompted us to assess the probable association between both conditions. This cross-sectional study recruited 153 patients scheduled to undergo coronary artery angiography. Patients were divided into two groups on the basis of coronary angiography results. Sixty-nine patients had coronary atherosclerosis and the 84 remaining patients were normal. Characteristics and pre-angiographic serum levels of triglyceride, low-density lipoprotein cholesterol, high-density lipoprotein, and Helicobacter.pylori IgG antibody were assessed in the patients and compared between the groups. Helicobacter.pylori infection occurred in 88 (57.51%) patients: 40 (58%) in the atherosclerotic group and 48 (57.1%) in the control group with no significant differences (P=0.918). Our multivariable analysis revealed that Helicobacter.pylori infection was not an independent predictive factor for coronary artery disease (P =0.915). Also, the prevalence of atherosclerosis risk factors with respect to the seropositive and seronegative Helicobacter.pylori infection was assessed in the case group, which showed no significant difference. Furthermore, the prevalence of seropositive Helicobacter.pylori infection in terms of the number of diseased coronary vessels was evaluated, this demonstrated no significant association between the number of the diseased vessels and Helicobacter.pylori infection. This study demonstrated that Helicobacter.pylori infection was not an independent predictive factor of atherosclerosis.

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Association of Helicobacter Pylori Infection in Patients Having Coronary Artery Disease

Nepalese Heart Journal ◽

10.3126/njh.v3i3.26054 ◽

2004 ◽

Vol 3 (3) ◽

pp. 7

Author(s):

A Ranjit ◽

CM Pathak ◽

K.L. Khanduja ◽

H.K. Ball ◽

D.K. Bhasin

Keyword(s):

Gastric Cancer ◽

Coronary Artery Disease ◽

Coronary Heart Disease ◽

Helicobacter Pylori ◽

Gastric Ulcer ◽

Coronary Artery ◽

Breath Test ◽

Urea Breath Test ◽

Artery Disease ◽

H Pylori

Helicobacter pylori usually cause a lifelong infection of gastric mucosa that may lead to gastric ulcer and later on gastric cancer. H, pylori infection and coronary heart disease are common conditions in late middle and old age. Recently, some studies have found casual association between H. Pylori infection and coronary artery disease, cerebrovascular disease and atherosclerosis. The study was done to detect the presence of H. Pylori in patients of coronary artery disease (CAD) by 14C-urea breath test and to find out any possible association between H. Pylori infection and coronary artery disease,

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Role of Helicobacter pylori specific heat shock protein-60 antibodies in the aetiology of coronary artery disease

Microbiology Research ◽

10.4081/mr.2012.e15 ◽

2012 ◽

Vol 3 (2) ◽

pp. 15

Author(s):

Mahadev D. Dixit ◽

Kishore G. Bhat ◽

Aruneshwari Dayal

Keyword(s):

Coronary Artery Disease ◽

Helicobacter Pylori ◽

Coronary Artery ◽

Heat Shock ◽

Heat Shock Protein ◽

Heat Shock Protein 60 ◽

Artery Disease ◽

H Pylori ◽

Antibody Levels

The role of chronic infections in causing coronary artery disease (CAD) has been investigated for the past several years. Among them, the role of Helicobacter pylori has stimulated keen interest. Though initial results were conflicting, there are growing data to support the role of H. pylori in CAD. The main mechanism of endothelial damage is hypothesized to be through molecular mimicry involving heat shock proteins. This study was designed to determine the prevalence of H.pylori and cytotoxin associated gene A (cagA) positive H.pylori infection in patients undergoing coronary artery bypass grafting (CABG) and the potential role of anti-H. pylori specific heat shock protein-60 (Hp-HSP-60) antibody response in these patients, for cardiac events. One hundred patients undergoing CABG and 100 controls were studied. The H.pylori infection and cagA status were determined serologically by enzyme-linked immunosorbent assay (ELISA). Hp-HSP-60 Immunoglobulin G (IgG) antibodies were estimated by using an in house ELISA. Although there was no difference in the prevalence of H.pylori infection in patients and controls (74% vs 70%), 58% of patients were infected with cagA positive H.pylori compared to 36% of controls (P=0.002). Mean systemic levels of Hp-HSP-60 IgG were also higher in patients than in controls (27.9 vs 18.7, P=0.0001). These antibody levels were also significantly higher in H.pylori positive patients (P=0.0001). There was a strong correlation between Hp-HSP-60 antibody levels and occurrence of myocardial infarction (P=0.003). CagA positive H.pylori infection may be associated with the development of CAD. High levels of Hp-HSP-60 antibodies may constitute a marker and/or concomitant pathogenic factor of the disease.

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