scholarly journals In vitro growth and indoleacetic acid production by Mesorhizobium loti SEMIA806 and SEMIA816 under the influence of copper ions

2017 ◽  
Vol 8 (2) ◽  
Author(s):  
Jéssica Dutra Vieira ◽  
Paulo Roberto Diniz Da Silva ◽  
Valdir Marcos Stefenon
Keyword(s):  
Contaminated Soils ◽  
Acid Production ◽  
Indoleacetic Acid ◽  
Copper Ions ◽  
Control Culture ◽  
Control Treatment ◽  
Symbiotic Interaction ◽  
Mesorhizobium Loti ◽  
Significant Difference

The indoleacetic acid produced by symbiotic bacteria is an important phytohormone signaling microbe-plant interaction, being therefore essential for rhizoremediation. In this study, the effect of different concentrations of copper ions on the bacterial growth and indoleacetic acid production was investigated in two strains of Mesorhizobium loti in in vitro conditions, aiming to determine critical concentrations of this heavy metal for rhizoremediation of contaminated soils using this bacterium. The experiment consisted on a control culture without copper and three treatments supplemented with 10 mg.L-1, 20 mg.L-1 or 50 mg.L-1 of CuSO4. For both strains, the growth stopped after 48h and no significant difference was observed across treatments. The production of indoleacetic acid by the control treatment without copper was significantly higher in comparison to the copper- containing treatments. Mesorhizobium loti SEMIA806 and SEMIA816 are resistant to up to 50 mg.L-1 of CuSO4 in the culture medium, presenting effective growth. The synthesis of indoleacetic acid was strongly reduced but not excluded by ions copper in the medium. So, it is expected that environmental copper found in the soil up to the concentration of 50 mg.L-1 will not preclude the symbiotic interaction between M. loti and leguminous host plant in rhizoremediation enterprises.

scholarly journals In Vitro Effects of Different Combinations of Phytohormones on Callus Induction from Different Explants of Cabbage Brassica Oleracea Var. Capitata L. Seedlings

2021 ◽  
pp. 3476-3486
Author(s):  
Alaa. M. Hasan ◽  
Ekhlas. A.J. ElKaaby ◽  
Rakad. M.Kh. AL-Jumaily
Keyword(s):  
Brassica Oleracea ◽  
Callus Induction ◽  
Basal Medium ◽  
Culture Conditions ◽  
Control Treatment ◽  
Fresh Weight ◽  
Superior Result ◽  
Significant Difference ◽  
In Vitro Effects

    The leading purpose of this work is the development of efficient culture conditions to induce calli from cabbage (Brassica oleracea var. capitata L.) under in vitro conditions. The mature seeds were surface sterilized with combinations of different concentrations of ethanol and NaOCl in different time durations and  were germinated on MS basal medium. The results revealed that the best sterilization method of cabbage seeds was by using 70% ethanol for one minute, followed by 15 min in 2% (NaOCl). Seedlings were used as donor sources for hypocotyls, cotyledon leaves, true leaves, and shoot tip explants. These explants were cultured on different combinations of cytokinins (TDZ, BAP, Ad) and auxins (IAA, NAA, 2, 4-D) then implanted in Murashige and Skoog (MS) media. 4 weeks after culturing, a significant difference was found among the explants in response to plant hormones. The maximum percentage of callus induction (100%) was using the combinations of 1 BAP + 1 2, 4-D, 1 BAP + 1 NAA, and 1 BAP + 2 2,4-D mg. l-1. In addition, explants responses varied and the hypocotyls showed a superior result (85.71 %) as compared to other explants. For callus fresh weight, the combination of 0.22 TDZ + 79.9 Ad mg. l-1    had a significant effect, causing the highest fresh weight (0.2745g), while control treatment gave the lowest mean of 0.0066 g. Data showed that cotyledon explants were significantly superior in giving highest callus fresh weight with the mean of 0.1723 g. On the other hand, hypocotyl explants gave the lowest mean, reaching 0.1542 g.

Development of prepubertal goat oocytes after their in vitro maturation and chemical activation

Zygote ◽  
2020 ◽  
Vol 28 (6) ◽  
pp. 447-452
Author(s):  
Seungbum Hong ◽  
Binoy S. Vettical ◽  
Nisar Ahmad Wani
Keyword(s):  
Culture Media ◽  
Chemical Activation ◽  
Control Treatment ◽  
Developmental Potential ◽  
Embryo Culture Medium ◽  
Significant Difference ◽  
Maturation Rate ◽  
Further Development ◽  
Oviductal Fluid

SummaryExperiments were conducted to study in vitro maturation of prepubertal goat oocytes and their developmental potential after chemical activation. In Experiment 1, cumulus–oocytes complexes collected from the ovaries of prepubertal goats slaughtered at a local abattoir were matured in vitro in TCM-199-based medium supplemented with 10 µg/ml luteinizing hormone (LH) (treatment 1) or 10 µg/ml LH + 0.1 mM l-cysteine (treatment 2). In Experiment 2, mature oocytes were activated with either 5 µM ionomycin or 7% ethanol. After 18 h, some oocytes were randomly fixed and stained to evaluate their chromatin status, while others were cultured in embryo culture medium to study their further development. In Experiment 3, oocytes activated with 5 µM ionomycin were cultured for 7 days in one of the four different culture media [Charles Rosenkrans medium (CR-1), TCM-199, potassium simplex optimization medium (KSOM) and synthetic oviductal fluid (SOF)] to study their developmental potential. The maturation rate in control, treatment 1, and treatment 2 media did not differ from each other (P > 0.05). However, the lowest degeneration of oocytes was observed in treatment 3 (P < 0.05) when compared with the other two groups. The proportion of activated oocytes was higher, while non-activated oocytes were lower in ionomycin group when compared with the group activated with ethanol (P < 0.05). The proportions of oocytes cleaved were 65.7, 56.8, 61.0 and 54.4% in CR-1, TCM-199, KSOM and SOF medium, respectively, with no significant difference. However, further development of cleaved oocytes was better in KSOM followed by SOF.

scholarly journals Influence of pesticide-treated seeds on survival of Mesorhizobium sp. Cicer, symbiotic efficiency and yield in chickpea

2012 ◽  
Vol 48 (No. 1) ◽  
pp. 37-43 ◽  
Author(s):  
Kunal ◽  
P. Sharma
Keyword(s):  
Grain Yield ◽  
Seed Treatment ◽  
Fungal Pathogens ◽  
Winter Season ◽  
Control Treatment ◽  
Symbiotic Efficiency ◽  
Effective Measure ◽  
Significant Difference ◽  
Uninoculated Control

Chemical seed protectants are used to reduce the adverse effects of seedling fungal pathogens or insect attack on legume pastures and crops. Chickpea seeds are also frequently treated with Mesorhizobium sp. Cicer inoculant to promote effective symbiotic nitrogen fixation (SNF), which seems to be a cost effective measure. The population of viable Mesorhizobium sp. Cicer on seeds of chickpea declined with time of storage (4&deg;C) in pesticide treated and untreated chickpea seeds in vitro. A significant reduction in chickpea rhizobia was observed in seed treatment with Captan followed by Endosulfan and Chlorpyrifos. In a field experiment during the winter season 2006&ndash;2008, no difference in the emergence count of chickpea plants was observed. Treatments inoculated with Mesorhizobium sp. Cicer alone or along with Captan, Chlorpyrifos or Endosulfan showed improved plant growth and symbiotic parameters (plant height, nodulation, leghaemoglobin content, and nitrogen content) in comparison with the uninoculated control treatment. Significantly higher grain yield (9.6%) was observed in the treatment inoculated with Mesorhizobium sp. Cicer alone as compared to the uninoculated control. A non-significant difference in grain yield among treatments where Mesorhizobium sp. Cicer along with a mixture of fungicide and insecticides was applied was observed in contrast to the Mesorhizobium sp. Cicer treatment. In conclusion, the recommended rates of fungicide and insecticides as seed treatment were not detrimental to chickpea-Mesorhizobium sp. Cicer symbiosis, hence they can be safely used to obtain higher productivity. &nbsp;

scholarly journals Effects of the bendazol fungicide on in vitro development of the nim (Azadirachta indica A. JUSS)

2016 ◽  
Vol 7 ◽  
Author(s):  
Elisabete Pacheco Dos Santos ◽  
Robson Antonio De Souza ◽  
Maria Jaislanny Lacerda e Medeiros ◽  
Gilberto Dias Alves ◽  
Laureen Michelle Houllou
Keyword(s):  
Culture Medium ◽  
Callus Formation ◽  
Production Costs ◽  
Control Treatment ◽  
Nodal Segment ◽  
Shoot Height ◽  
Significant Difference ◽  
Lateral Bud ◽  
Vitro Development

Two experiments were conducted to evaluate Bendazol fungicidal effects in neem micropropagation. In these experiments, the nodal segment explants from <em>in vitro</em> plants were used. In the first experiment, the explants remained in DKW culture medium for a period of 30 days containing different concentrations of Bendazol (M1 -50, M2 - 100, M3 - 200, and M4 - 400 mg.L<sup>-1</sup>). The control treatment (M0) was prepared with DKW medium + BAP (0.225 mg.L<sup>-1</sup>). In the second experiment, the explants were maintained for only one week in media supplemented with Bendazol or BAP, and then they were transferred and kept in free Bendazol/BAP media for three weeks. In each experiment, the design was completely randomized with five treatments, 10 replicates per treatment, and one explant/cultivation flask.  The variables analyzed included the formation of calluses and roots, lateral bud development, shoot height, contamination and plant death. There was no significant difference in tree variables (shoot, callus formation and shoot height) between treatments in both experiments. There was no death, plant contamination and rooting during the experiment. The results indicate that Bendazol can be used at low doses for <em>in vitro</em> neem cloning thereby replacing BAP and ultimately reducing production costs.

scholarly journals Chemical Disinfestation for Sowing and in Vitro Re-cultivation of Orchids and its Influence on the Seedlings Nutrition

2017 ◽  
Vol 5 (4) ◽  
pp. 65
Author(s):  
Andréia Aline Fontes ◽  
Victor Hugo Alvarez V. ◽  
Sarah Vieira Novais ◽  
Wagner Campos Otoni ◽  
André Ferreira Santos ◽  
...  
Keyword(s):  
Laminar Flow ◽  
In Vitro Propagation ◽  
Dry Matter ◽  
Culture Media ◽  
Flow Chamber ◽  
Control Treatment ◽  
Significant Difference ◽  
Different Types ◽  
Dry Matter Weight

The use of a commercial bleach solution (CB) for in vitro propagation of orchids has proven to be an efficient technique and an alternative to the use of the autoclave and laminar flow chamber (LFC). The aim of this study was to evaluate the use of CB in disinfestation of culture media (CM) for seeding and for re-cultivation of orchid plantlets and its effect in different types of flasks and on the nutritional state of the plantlets. In the seeding experiment, seeds of Laelia tenebrosa Rolfe were used, with evaluation of three types of flasks; five concentrations of CB applied to the CM of 2, 5, 10, 20 and 40 mL L-1 and the control treatment. In the re-cultivationexperiment, Cattleya kerri Brieger & Bicalho plantlets were used. The rates of CB added to the CM were 2, 5, 10, 20, 30, 35 and 38 mL L-1 in polypropylene containers, and the rates sprayed on the plantlets at re-cultivation were 5, 12.5, 25, 50, 75, 87.5 and 95 mL L-1. The proportion of germinated seeds and the dry matter weight of the protocorms were greater in the control treatment. The increasing rates of CB added to the CM had worse results for germination in glass flasks. In re-cultivation, there was no significant difference in the growth of the plantlets between the autoclaved flasks and those disinfested in CB.Keymessage: Both orchid seeding and re-cultivation of seedlings can be carried out entirely and efficiently without the use of an autoclave and laminar flow chamber, using chemical disinfection with commercial bleach.

Effect of Honey on the Growth of and Acid Production by Human Intestinal Bifidobacterium spp.: An In Vitro Comparison with Commercial Oligosaccharides and Inulin

2002 ◽  
Vol 65 (1) ◽  
pp. 214-218 ◽  
Author(s):  
S. KAJIWARA ◽  
H. GANDHI ◽  
Z. USTUNOL
Keyword(s):  
Growth Rate ◽  
High Pressure ◽  
Acid Production ◽  
Specific Growth ◽  
Control Treatment ◽  
Acetic Acid Production ◽  
End Products ◽  
In Vitro Comparison ◽  
Acetic Acids

Five human intestinal Bifidobacterium spp., B. longum, B. adolescentis, B. breve, B. bifidum, and B. infantis, were cultured in reinforced clostridial medium (control) and in reinforced clostridial medium supplemented with 5% (wt/vol) honey, fructooligosaccharide (FOS), galactooligosaccharide (GOS), and inulin. Inoculated samples were incubated anaerobically at 37°C for 48 h. Samples were collected at 12-h intervals and examined for specific growth rate. Levels of fermentation end products (lactic and acetic acids) were measured by high-pressure liquid chromatography. Honey enhanced the growth of the five cultures much like FOS, GOS, and inulin did. Honey, FOS, GOS, and inulin were especially effective (P &lt; 0.05) in sustaining the growth of these cultures after 24 h of incubation as compared with the control treatment. Overall, the effects of honey on lactic and acetic acid production by intestinal Bifidobacterium spp. were similar to those of FOS, GOS, and inulin.

scholarly journals Rumen Fermentation Characteristics of West African Dwarf Sheep Fed Supplementary Diets Containing Water-Washed Neem (azadirachtaindica A. Juss) Fruit and Garlic (Allium sativum)

2016 ◽  
Vol 3 (8) ◽  
pp. 135
Author(s):  
Joshua Abolade Opeyemi ◽  
Ajayi Adeola
Keyword(s):  
Propionic Acid ◽  
Rumen Fermentation ◽  
West African ◽  
Control Treatment ◽  
Panicum Maximum ◽  
West African Dwarf ◽  
Significant Difference ◽  
West African Dwarf Sheep ◽  
Dwarf Sheep

An experiment was conducted for 37 days for the purpose of investigating Rumen Fermentation Characteristics of West African Dwarf Sheep Fed supplementary diets containing Water-Washed Neem (Azadirachtaindica a. Juss0 Fruit and garlic (Alliumsativum). The experimental design used was a completely randomized design and the obtained data were subjected to analysis of variance (ANOVA). 12 West African dwarf Sheep were randomly allotted into 4 treatments of 3 replicates per treatment, these treatments were supplements containing 0%, 5%, 10% neem and 1.25% garlic. Panicum maximum was fed as the basal diet. The basal and supplementary diets were fed at ratio 50:50 and at 3% body weight on dry matter bases. At the end of 37 days feeding trial, rumen liquor from the experimental animals were collected for rumen fermentation characteristics and in vitro gas production. The pH of the rumen liquor had values ranging from 6.30 - 7.00 and there was a significant difference between the 4 treatments. Treatment IV had the highest acetic acid and butyric acid production while treatment II had the lowest. However, there was no significant difference amongst the 4 treatments. Propionic acid was highest for treatment I while treatment II had the lowest value. For the propionic acid, the treatment that had no inclusion of neem and garlic (the control treatment) had the highest mean value for the study. The in vitro production obtained for a standard feed sample incubated in the rumen liquor obtained from the 4 treatments were significantly affected by the inclusion of neem and garlic. It is then concluded that the inclusion of neem at 10% and garlic at 1.25% in the supplementary feed of WAD sheep will significantly reduce methane production and support ruminant production.

scholarly journals Micropropagation of Vanda tricolor Lindl. var. suavis with various concentrations of organic growth supplements

2021 ◽  
Vol 886 (1) ◽  
pp. 012004
Author(s):  
M Tuwo ◽  
A I Latunra ◽  
E T Ana
Keyword(s):  
Tissue Culture ◽  
Culture Media ◽  
Good Alternative ◽  
Control Treatment ◽  
Tissue Culture Media ◽  
Organic Growth ◽  
Randomized Design ◽  
Significant Difference ◽  
Number Of Leaves

Abstract Plant propagation through in vitro culture is increasingly being used to produce hybrid orchids. Plant tissue culture provides a good alternative to produce plants in large numbers in a short time. The provision of organic growth supplements in tissue culture media plays an important role as a substitute for growth regulators in stimulating the growth of explants. In this study, young coconut water, banana extract (cv. ambon), and tomato extract were used to stimulate the growth of the Vanda tricolor Lindl. Var suavis protocorm. Data were analyzed using a completely randomized design (CRD) with five replications using the Kruskal-Wallis test and continued with the Mann-Whitney test if there was a significant difference between each treatment and its concentration. Parameters observed were the percentage of the number of shoots and the number of leaves. The results showed that there was a significant difference between the treatments given to the number of leaves. Mann-Whitney further test results on the number of leaves showed a significant difference in the banana extract treatment and the control treatment.

LDL-Oxidation und -Subgruppenverteilung bei normo- und hypertensiven Patienten mit und ohne familiäre hypertonische Belastung

VASA ◽  
2002 ◽  
Vol 31 (2) ◽  
pp. 75-80
Author(s):  
Brockes ◽  
Locher ◽  
Böttcher ◽  
Schmitz ◽  
Vetter
Keyword(s):  
Copper Ions ◽  
Density Lipoprotein ◽  
Oxidative Modification ◽  
Ldl Oxidation ◽  
Control Group ◽  
Hypertensive Patients ◽  
Family Histories ◽  
Increased Risk ◽  
Significant Difference

Objectives: Oxidative modification of low-density-lipoprotein (LDL) increases its atherogenic potential to induce the accumulation of lipids and cells in the vascular wall. Patients have different lipoprotein profiles according to their LDL-subgroup pattern. The subgroup of LDL, which is most susceptible to oxidation, is most likely the dense LDL3 subfraction. In order to study an assumed association between hypertension, LDL subgroup distribution and the susceptibility of LDL to oxidation, 14 normotensive patients without family histories of hypertension (NT), 13 normotensive patients with family histories of hypertension (NT-FH), 10 hypertensive patients without family histories of (HT) and 11 hypertensive patients with family histories of hypertension (HT-FH) were evaluated. Patients and methods: LDL was oxidatively modified by incubation with copper ions (1.6 muM/L). The course of LDL-oxidation was measured in vitro by continuous photometric monitoring and the quantitative distribution of 3 LDL-subgroups by capillary isotachophoresis (ITP). Results: The lag-phases of NT-FH and hypertensive patients were shorter than those of the control group (NT: 116 ± 36 minutes; NT-FH 92 ± 32 minutes, p < 0.05; HT: 95 ± 41 minutes; HT-FH: 76 ± 33 minutes, p < 0.05). Compared to NT a significant difference in the relative preponderance of LDL3 subgroup was observed for HT-FH (23.5 ± 4.6% versus NT: 19.3 ± 6.6%), additionally, statistical analysis showed a similar trend amongst the other patient groups (NT-FH: 20.4 ± 7.4%, HT: 21.4 ± 4.6%). Conclusions: The increased occurence of the LDL3 subgroup might contribute to a higher susceptibility to LDL oxidation and therefore create an increased risk of vascular disease in the genotypic and phenotypic hypertensive patient population.

Predictive Model for Live Birth at 12 Months After Starting In-Vitro Fertilization Treatment

MedPharmRes ◽  
2018 ◽  
Vol 2 (2) ◽  
pp. 5-20
Author(s):  
Vu Ho ◽  
Toan Pham ◽  
Tuong Ho ◽  
Lan Vuong
Keyword(s):  
In Vitro Fertilization ◽  
Live Birth ◽  
Cox Regression ◽  
Financial Burden ◽  
Oocyte Retrieval ◽  
Operating Characteristics ◽  
Vitro Fertilization ◽  
Cox Regression Analysis ◽  
Significant Difference

IVF carries a considerable physical, emotional and financial burden. Therefore, it would be useful to be able to predict the likelihood of success for each couple. The aim of this retrospective cohort study was to develop a prediction model to estimate the probability of a live birth at 12 months after one completed IVF cycle (all fresh and frozen embryo transfers from the same oocyte retrieval). We analyzed data collected from 2600 women undergoing in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) at a single center in Vietnam between April 2014 and December 2015. All patients received gonadotropin-releasing hormone (GnRH) antagonist stimulation, followed by fresh and/or frozen embryo transfer (FET) on Day 3. Using Cox regression analysis, five predictive factors were identified: female age, total dose of recombinant follicle stimulating hormone used, type of trigger, fresh or FET during the first transfer, and number of subsequent FET after the first transfer. The area under the receiver operating characteristics curve for the final model was 0.63 (95% confidence interval [CI] 0.60‒0.65) and 0.60 (95% CI 0.57‒0.63) for the validation cohort. There was no significant difference between the predicted and observed probabilities of live birth (Hosmer-Lemeshow test, p > 0.05). The model developed had similar discrimination to existing models and could be implemented in clinical practice.

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