Effect of Honey on the Growth of and Acid Production by Human Intestinal Bifidobacterium spp.: An In Vitro Comparison with Commercial Oligosaccharides and Inulin

2002 ◽  
Vol 65 (1) ◽  
pp. 214-218 ◽  
Author(s):  
S. KAJIWARA ◽  
H. GANDHI ◽  
Z. USTUNOL
Keyword(s):  
Growth Rate ◽  
High Pressure ◽  
Acid Production ◽  
Specific Growth ◽  
Control Treatment ◽  
Acetic Acid Production ◽  
End Products ◽  
In Vitro Comparison ◽  
Acetic Acids

Five human intestinal Bifidobacterium spp., B. longum, B. adolescentis, B. breve, B. bifidum, and B. infantis, were cultured in reinforced clostridial medium (control) and in reinforced clostridial medium supplemented with 5% (wt/vol) honey, fructooligosaccharide (FOS), galactooligosaccharide (GOS), and inulin. Inoculated samples were incubated anaerobically at 37°C for 48 h. Samples were collected at 12-h intervals and examined for specific growth rate. Levels of fermentation end products (lactic and acetic acids) were measured by high-pressure liquid chromatography. Honey enhanced the growth of the five cultures much like FOS, GOS, and inulin did. Honey, FOS, GOS, and inulin were especially effective (P < 0.05) in sustaining the growth of these cultures after 24 h of incubation as compared with the control treatment. Overall, the effects of honey on lactic and acetic acid production by intestinal Bifidobacterium spp. were similar to those of FOS, GOS, and inulin.

Growth and Viability of Commercial Bifidobacterium spp. in Honey-Sweetened Skim Milk

2001 ◽  
Vol 64 (11) ◽  
pp. 1775-1779 ◽  
Author(s):  
Z. USTUNOL ◽  
H. GANDHI
Keyword(s):  
Growth Rate ◽  
High Performance ◽  
Specific Growth ◽  
Growth Promotion ◽  
Skim Milk ◽  
Bifidobacterium Bifidum ◽  
Refrigerated Storage ◽  
Dry Milk ◽  
End Products ◽  
Acetic Acids

Two commercial Bifidobacterium bifidum (Bf-1 and Bf-6) were cultured in 12% (wt/vol) reconstituted nonfat dry milk (NDM) containing 5% (wt/wt) honey, sucrose, fructose, or glucose. Inoculated samples were incubated anaerobically at 37°C for 48 h. Samples were collected at 12-h intervals and examined for (i) specific growth rate, (ii) pH, and (iii) levels of fermentation end products (lactic and acetic acids) as measured by high-performance liquid chromatography(HPLC). Viability of the organisms during 28 days of refrigerated storage at 4°C was also assessed at 7-day intervals. Growth promotion and acid production were greatest when Bf-1 and Bf-6 were grown in the presence of honey. For both Bf-1 and Bf-6, retention of viability was greatest up to 14 days of refrigerated storage at 4°C when they were grown and stored in the presence of honey compared to other sweeteners.

scholarly journals In vitro growth and indoleacetic acid production by Mesorhizobium loti SEMIA806 and SEMIA816 under the influence of copper ions

2017 ◽  
Vol 8 (2) ◽  
Author(s):  
Jéssica Dutra Vieira ◽  
Paulo Roberto Diniz Da Silva ◽  
Valdir Marcos Stefenon
Keyword(s):  
Contaminated Soils ◽  
Acid Production ◽  
Indoleacetic Acid ◽  
Copper Ions ◽  
Control Culture ◽  
Control Treatment ◽  
Symbiotic Interaction ◽  
Mesorhizobium Loti ◽  
Significant Difference

The indoleacetic acid produced by symbiotic bacteria is an important phytohormone signaling microbe-plant interaction, being therefore essential for rhizoremediation. In this study, the effect of different concentrations of copper ions on the bacterial growth and indoleacetic acid production was investigated in two strains of Mesorhizobium loti in in vitro conditions, aiming to determine critical concentrations of this heavy metal for rhizoremediation of contaminated soils using this bacterium. The experiment consisted on a control culture without copper and three treatments supplemented with 10 mg.L-1, 20 mg.L-1 or 50 mg.L-1 of CuSO4. For both strains, the growth stopped after 48h and no significant difference was observed across treatments. The production of indoleacetic acid by the control treatment without copper was significantly higher in comparison to the copper- containing treatments. Mesorhizobium loti SEMIA806 and SEMIA816 are resistant to up to 50 mg.L-1 of CuSO4 in the culture medium, presenting effective growth. The synthesis of indoleacetic acid was strongly reduced but not excluded by ions copper in the medium. So, it is expected that environmental copper found in the soil up to the concentration of 50 mg.L-1 will not preclude the symbiotic interaction between M. loti and leguminous host plant in rhizoremediation enterprises.

scholarly journals Comparison of antibacterial effect of seven 1-monoglycerides on food-borne pathogens or spoilage bacteria

2011 ◽  
Vol 80 (1) ◽  
pp. 29-39 ◽  
Author(s):  
Leona Buňková ◽  
František Buňka ◽  
Rahula Janiš ◽  
Jiří Krejčí ◽  
Iva Doležálková ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Growth Rate ◽  
Specific Growth ◽  
Lag Time ◽  
Gram Negative Bacteria ◽  
Inhibitory Effects ◽  
Gram Negative ◽  
Food Borne Pathogens ◽  
Food Borne

The aim of this study was to compare under the same conditionsin vitrothe inhibitory effects of seven 1-monoglycerides (MAG) containing fatty acids with a medium chain on ten strains of food-borne pathogens or spoilage gram-positive and gram-negative bacteria (Bacillus cereus, Bacillus subtilis, Enterococcus faecalis, Micrococcus luteus, Staphylococcus aureus, Citrobacter freundii, Escherichia coli, Proteus mirabilis, Salmonella entericaser.Enteritidis andPseudomonas aeruginosa) and on their growth indicatos. The inhibitory effect of MAGs (monocaprylin, monocaprin, monolaurin, monomyristin, monopalmitin, MAG of undecanoic and 10-undecenoic acids) at a concentration of 25 – 1500 mg·l-1was observed. Growth of bacteria in the presence of MAG was studied by means of optical density of bacteria for 24 h. The data were modelled through a Gompertz equation and the lag-time, the maximum specific growth rate and the maximal value reached were calculated. MAGs inhibited mainly the growth of gram-positive bacteria, which was shown by the extended lag-time, decrease in specific growth rate and decrease in cell density. Inhibitory effects of tested MAGs could be ranked from point of view of the minimum inhibitory concentration: MAG-C12:0 > MAG-C11:0 > MAG-C10:0 > MAG-C14:0 > MAG-C11:1 > MAG-C8:0 > MAG-C16:0.In vitro, no significant inhibitory effects of 1-monoglycerides, with the exception of the highest concentrations applied, on the growth of gram-negative bacteria were detected. The main contribution of this study is to compare the effects of several MAG containing fatty acids with a medium chain under the same conditions on the growth indicators of bacteria.

scholarly journals Soymilk residue (okara) as a natural immobilization carrier forLactobacillus plantarumcells enhances soymilk fermentation, glucosidic isoflavone bioconversion, and cell survival under simulated gastric and intestinal conditions

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2701 ◽  
Author(s):  
Xia Xiudong ◽  
Wang Ying ◽  
Liu Xiaoli ◽  
Li Ying ◽  
Zhou Jianzhong
Keyword(s):  
Specific Growth ◽  
Cell Immobilization ◽  
Low Ph ◽  
Plate Count ◽  
Lag Phase ◽  
Titrable Acidity ◽  
Colony Forming Units ◽  
Kinetics Of ◽  
Acetic Acids

Cell immobilization is an alternative to microencapsulation for the maintenance of cells in a liquid medium. However, artificial immobilization carriers are expensive and pose a high safety risk. Okara, a food-grade byproduct from soymilk production, is rich in prebiotics. Lactobacilli could provide health enhancing effects to the host. This study aimed to evaluate the potential of okara as a natural immobilizer forL. plantarum70810 cells. The study also aimed to evaluate the effects of okara-immobilizedL. plantarum70810 cells (IL) on soymilk fermentation, glucosidic isoflavone bioconversion, and cell resistance to simulated gastric and intestinal stresses. Scanning electron microscopy (SEM) was used to show cells adherence to the surface of okara. Lactic acid, acetic acid and isoflavone analyses in unfermented and fermented soymilk were performed by HPLC with UV detection. Viability and growth kinetics of immobilized and freeL. plantarum70810 cells (FL) were followed during soymilk fermentation. Moreover, changes in pH, titrable acidity and viscosity were measured by conventional methods. For in vitro testing of simulated gastrointestinal resistance, fermented soymilk was inoculated with FL or IL and an aliquot incubated into acidic MRS broth which was conveniently prepared to simulate gastric, pancreatic juices and bile salts. Survival to simulated gastric and intestinal stresses was evaluated by plate count of colony forming units on MRS agar. SEM revealed that the lactobacilli cells attached and bound to the surface of okara. Compared with FL, IL exhibited a significantly higher specific growth rate, shorter lag phase of growth, higher productions of lactic and acetic acids, a faster decrease in pH and increase in titrable acidity, and a higher soymilk viscosity. Similarly, IL in soymilk showed higher productions of daizein and genistein compared with the control. Compared with FL, IL showed reinforced resistance to simulatedgastric and intestinal stresses in vitro that included low pH, low pH plus pepsin, pancreatin, and bile salt. Our results indicate that okara is a new potential immobilization carrier to enhance the growth and glucosidic isoflavone bioconversion activities ofL. plantarumin soymilk and improve cell survivability following simulated gastric and intestinal conditions.

Co-inoculation of nitrogen-fixing and phosphate-solubilizing bacteria to promote growth, yield and nutrient uptake in chickpea

2007 ◽  
Vol 55 (3) ◽  
pp. 315-323 ◽  
Author(s):  
P. Wani ◽  
M. Khan ◽  
A. Zaidi
Keyword(s):  
Seed Yield ◽  
N Uptake ◽  
Growth Yield ◽  
Luria Bertani ◽  
Control Treatment ◽  
Grain Protein ◽  
Phosphate Solubilizing Bacteria ◽  
Acetic Acid Production ◽  
Phosphate Solubilizing

A total of 32 bacterial isolates including Mesorhizobium (N=10), Azotobacter (N=12) and phosphate-solubilizing bacteria (N=10) were isolated and tested for siderophore, HCN, ammonia, indole acetic acid production and phosphate solubilization in vitro . The bacterial cultures were positive for siderophore, HCN and ammonia. Among the isolates, M. ciceri RC3 and A. chrococcum A4 displayed 35 and 14 μg ml −1 of IAA, respectively, whereas Bacillus produced 19 ( Bacillus PSB1) and 17 μg ml −1 ( Bacillus PSB10) of IAA in Luria Bertani broth. The diameter of the P solubilization zone varied between 4 ( Bacillus PSB1) and 5 mm ( Bacillus PSB10) and a considerable amount of tricalcium phosphate (7 and 8 μg ml −1 by Bacillus PSB1 and Bacillus PSB10, respectively) was released in liquid medium, with a concomitant drop in pH. The effects of N 2 -fixing and PS bacteria on the growth, chlorophyll content, seed yield, grain protein and N uptake of chickpea plants in field trials varied considerably between the treatments. Nodule number and biomass were significantly greater at 90 days after sowing (DAS), decreasing by 145 DAS. Seed yield increased by 250% due to inoculation with M. ciceri RC3 + A. chroococcum A4 + Bacillus PSB10, relative to the control treatment. Grain protein content ranged from 180 ( Bacillus PSB1) to 309 ng g −1 ( M. ciceri RC3 + A. chroococcum A4 + Bacillus PSB10) in inoculated chickpea. The N contents in roots and shoots differed considerably among the treatments.

scholarly journals Mathematical Modeling of Lactobacillus paracasei CBA L74 Growth during Rice Flour Fermentation Performed with and without pH Control

2021 ◽  
Vol 11 (7) ◽  
pp. 2921
Author(s):  
Rosa Colucci Cante ◽  
Marianna Gallo ◽  
Federica Nigro ◽  
Francesca Passannanti ◽  
Andrea Budelli ◽  
...  
Keyword(s):  
Mathematical Modeling ◽  
Growth Rate ◽  
Lactic Acid ◽  
Specific Growth Rate ◽  
Biomass Production ◽  
Acid Production ◽  
Specific Growth ◽  
Lactic Acid Production ◽  
Rice Flour ◽  
Ph Control

The mathematical modeling of fermentation processes allows for the formulation of predictions about the kinetics of biomass growth and metabolite production as well as setting or verifying the best operative conditions in view of the economical convenience of the process. For this purpose, we performed a kinetic study of a rice flour fermentation process using Lactobacillus paracasei CBA L74 with and without pH control; the pH value was set to 5.8 under pH control. Monod, Logistic, and Contois models were proposed to describe the bacterial growth rate in both conditions. The best mathematical model, which was able to describe the experimental data obtained without pH control, was the Contois model, as the specific growth rate was influenced by both the glucose reduction (from 14.31 g/L to 10.22 g/L) and the biomass production (2 log growth) that occurred during fermentation. Conversely, when pH control was implemented, both Monod and Contois models satisfactorily described the specific growth rate trend. The estimated kinetic parameters confirmed that biomass production (2 log growth) and glucose consumption (from 14.31 g/L to 6.06 g/L) did not affect the microorganism’s growth capacity when the fermenting medium was maintained at an optimal pH. The lactic acid production rate described by the Luedeking–Piret model did not appear to be linked to growth in the absence of pH control while, on the other hand, this model was unsuitable for describing the experimental lactic acid concentration when pH control was applied. The kinetic modeling of lactic acid production and the percentage of added glucose in the protocol with controlled pH will be optimized in the future.

scholarly journals IMPROVEMENT IN THE GROWTH PERFORMANCE OF TIGER GROUPER Epinephelus fuscoguttatus (Forsskal, 1775) BY PROBIOTIC MICROCAPSULES, Bacillus sp. D2.2

AQUASAINS ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 853
Author(s):  
Ignatius Sandra Setyabudi ◽  
Esti Harpeni ◽  
Wardiyanto Wardiyanto
Keyword(s):  
Growth Rate ◽  
Survival Rate ◽  
Specific Growth ◽  
Protein Digestibility ◽  
Control Treatment ◽  
Bacillus Sp ◽  
Absolute Weight ◽  
Epinephelus Fuscoguttatus ◽  
Tiger Grouper ◽  
Pass Through

Probiotics in the fish's body are able to optimize the nutrition of feed given for growth. Microcapsule techniques can protect probiotics as they pass through the digestive tract. This study aims to study the increase in growth of tiger grouper fish fed with probiotic Bacillus sp. Microcapsules. D2.2 with different dosages. The study was conducted in May - June (30 days) with 5 treatments and 3 replications. The treatments applied are K- (feed without probiotics), K + (feed + liquid probiotics), A (feed + probiotic microcapsules 1 gram / kg of feed), B (feed + probiotic microcapsules 2 gram / kg of feed), C (feed + probiotic microcapsules 3 grams / kg of feed). The following are the highest observations, namely absolute weight gain (23.8 ± 0.7) grams, specific growth rate (1.35 ± 0.04)% /day, feed covertion ratio (FCR) (1.3 ± 0.1) ), survival rate (83 ± 2.9)% and protein digestibility 90.56% while the lowest value is in the control treatment. The results showed that the administration of probiotic microcapsules was able to significantly increase the growth of tiger grouper fish compared to control treatments.

scholarly journals Effect of Adding BIOM-S Probiotic on Survival and Growth Rate of Nile Tilapia (Oreochromis niloticus)

2018 ◽  
pp. 1-6
Author(s):  
Yuli Andriani ◽  
Taufik Ikhsan Kamil ◽  
Irfan Zidni ◽  
Iskandar . ◽  
Titin Herawati
Keyword(s):  
Growth Rate ◽  
Survival Rate ◽  
Specific Growth Rate ◽  
Nile Tilapia ◽  
Specific Growth ◽  
Culture Media ◽  
Control Treatment ◽  
Randomized Design ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Survival And Growth

This research was conducted in May-June 2017 in Hatchery Indoor Maksudi, Astanaanyar, Bandung. This research aims to find out the optimal BIOM-S probiotic on culture media of Nile tilapia, and the influence to survival rate and growth rate of Nile tilapia. This research was conducted by an experiment using Completely Randomized Design (CRD). This research consisted of five treatments and three replications, which were treatment A (control), treatment B (giving probiotic with 0,6 ml/L concentration), treatment C (giving probiotic with 0,8 ml/L concentration), treatment D (giving probiotic with ,0 ml/L concentration), and treatment E (giving probiotic with 1,2 ml/L concentration). The parameters in this research were the survival rate and specific growth rate. Survival rate and specific growth rate used data analysis with F test to find out the influence of each treatment. The concentration of optimal probiotic based on the results was 0.8 ml/L, it produced the highest survival rate for 81.67% and the specific growth rate for 0.039%.

scholarly journals Impact of Pseudomonas spp. on Plant Growth, Lytic Enzymes and Secondary Metabolites Production

2021 ◽  
Vol 3 ◽  
Author(s):  
Rajesh R. Waghunde ◽  
Ambalal N. Sabalpara
Keyword(s):  
Plant Growth ◽  
Acid Production ◽  
Plant Pathogens ◽  
Growth Promotion ◽  
Root Weight ◽  
Bacterial Strains ◽  
Lytic Enzymes ◽  
Acetic Acid Production ◽  
Metabolites Production

Seven strains of Pseudomonas spp. were isolated from the south Gujarat region of India. Antifungal and bacterial activities of bacterial strains were evaluated against important plant pathogens in vitro, among them, PaRS was found most effective. The indole acetic acid production was recorded in all isolated Pseudomonas spp. Seed treatment with PaRS at 6 g/kg was significantly superior over other treatments for plant growth promotion (germination, shoot and root length, shoot and root weight, vigor index, and both shoot and root colonization) under standard roll towel method and pot conditions. The maximum siderophore production was observed in PaRS and medium production in PfRB, PaNS, and PfNC. PaNS and PaRS strains recorded strong HCN production but moderate production recorded in PaWP, PaWS, and PfNC. The maximum phosphate solubalization zone (22 mm) was found in PaRS. PaRS recorded maximum chitinase, ß-1,3-glucanase activity, hydrogen cyanide, and salicylic acid production as compared to other strains.

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