Anti-inflammatory effect of the methanol extract from Anthocephalus cadamba stem bark in animal models

Background: Anthocephalus cadamba (Rebox)Miq. (Rubiaceae) is widely distributed throughout the greater part of India, especially at low levels in wet place. Traditionally the bark is used as tonic, febrifuge and to reduce the pain and inflammation. The anti-inflammatory effect of methanol extract obtained from Anthocephalus cadamba aerial parts, MEAC, were investigated in this study. Design and methods: The effects of MEAC on the acute and chronic phases of inflammation were studied in carrageenan, dextran and mediators (histamine and serotonin) induced paw edema and cotton pallet-induced granuloma, respectively. The anti-edema effect of MEAC was compared with 10 mg/kg of indomethacin orally. Results: The results suggested that MEAC possess potent anti-inflammatory activity. The acute inflammatory model showed that all the doses of MEAC effectively suppressed the edema produced by histamine, so it may be suggested that its anti-inflammatory activity is possibly backed by its antihistaminic activity. In chronic inflammatory model the effect may be due to the cellular migration to injured sites and accumulation of collagen and mucopolysaccharide. Conclusions: On the basis of these findings, it may be inferred that Anthocephalus cadamba is an anti-inflammatory agent and the results are in agreement with its traditional use.

Download Full-text

Anti-Inflammatory Activity and Composition ofSenecio salignusKunth

BioMed Research International ◽

10.1155/2013/814693 ◽

2013 ◽

Vol 2013 ◽

pp. 1-4 ◽

Cited By ~ 4

Author(s):

Cuauhtemoc Pérez González ◽

Roberto Serrano Vega ◽

Marco González-Chávez ◽

Miguel Angel Zavala Sánchez ◽

Salud Pérez Gutiérrez

Keyword(s):

Medicinal Plant ◽

Methanol Extract ◽

Chloroform Extract ◽

Inflammatory Activity ◽

Paw Edema ◽

Active Fraction ◽

Ear Edema ◽

Methanol Extracts ◽

Anti Inflammatory ◽

Inflammatory Effect

We investigated the anti-inflammatory activity ofSenecio salignus. This medicinal plant is often used in Mexico for the treatment of fever and rheumatism. Chloroform and methanol extracts of the plant were tested on 12-O-tetradecanoylphorbol-13-acetate- (TPA-) induced edema in mice ears. The methanol extract of the plant inhibited edema by36±4.4% compared with the control, while the chloroform extract exhibited an even greater level of inhibition (64.1%). The chloroform extract was then fractionated, and the composition of the active fraction was determined by GC-MS. The anti-inflammatory activity of this fraction was then tested on TPA-induced ear edema in mice, and we found that the active fraction could inhibit edema by 46.9%. The anti-inflammatory effect of the fraction was also tested on carrageenan-induced paw edema in rats at doses of 100 mg/kg; a58.9±2.8% reduction of the edema was observed 4 h after administration of carrageenan, and the effect was maintained for 5 h.

Download Full-text

Pharmacognostic Screening and Anti-inflammatory Investigation of the Methanol extract of stem bark of Blighia unijugata Baker (Sapindaceae)

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v10i4.4167 ◽

2020 ◽

Vol 10 (4) ◽

pp. 146-152

Author(s):

Felix N. Osuala ◽

Uchenna E. Odoh ◽

VictoriaC. Onuigbo ◽

Sylvester C. Ohadoma

Keyword(s):

Methanol Extract ◽

Lethal Dose ◽

Numerical Data ◽

Stem Bark ◽

Water Soluble ◽

Herbal Remedies ◽

Albino Rats ◽

Standard Drug ◽

Anti Inflammatory ◽

Inflammatory Effect

Introduction: Blighia unijugata is a potent medicinal plant extensively employed in traditional herbal remedies for the treatment of various diseases such as fever, fertility, inflammation, hypertension, migraine and treatment of infections due to microorganisms. Aim: This work, investigates the pharmacognostic screening and anti-inflammatory activities of the methanol extract of B. unijugata stem bark. Method: Collection, drying, pulverization, and methanol extraction of the stem bark were done accordingly. The screening of phytochemical constituents and Pharmacognostic numerical data were carried out. The chromatographic analysis was carried out using TLC. The acute toxicity was determined using Lorke’s method. Methanol extract was investigated for anti-inflammatory effect in albino rats using egg-induced hind paw oedema at doses of 200, 400 and 600mg/kg body weight respectively. Result: The macroscopical investigation showed the stem bark outer layer is greyish and, the inner layer is pale reddish brown, disagreeable odour, bitter, Microscopical screening revealed the presence of starch grains, trichomes and sclerenchyma cells. Chemomicroscopic result revealed the presence of lignin, starch, calcium oxalate, cellulose, stone cells. Qualitative phytochemical screening of the powdered bark showed the presence of saponins, steroids, tannins, and resins. Numerical data: moisture content/ weight loss on drying gave value of 7.7%, percentage yield of 6.7%, alcoholic soluble extractive 5.6%, water soluble extractive 6.3%, total ash of 7.1%, acid insoluble ash 0.57% and water soluble ash 4.56%. The chromatographic screening results were close compared with the standard drug. Toxicity test established the lethal dose of greater than 5000mg/kg. There was a significant inhibition of the edema p>0.05, the presence of various bioactive constituents may have contributed to the anti-inflammatory properties of the plant extract. Conclusion: The result of this study confirms that the barks of B. unijugata have anti-inflammatory effect and justifies the use as traditional treatment of inflammation and pain. Keywords: Blighia unijugata, Inflammation, Inflammatory agents, Anti inflammatory

Download Full-text

Effects of Methanol Extract of Piper chaba Stem Bark on Acute Inflammation in rats

Faridpur Medical College Journal ◽

10.3329/fmcj.v7i1.10294 ◽

2012 ◽

Vol 7 (1) ◽

pp. 26-28

Author(s):

F Begum ◽

ZA Begum ◽

MR Uddin ◽

AHMZ Haider ◽

RC Barman

Keyword(s):

Body Weight ◽

Acute Inflammation ◽

Methanol Extract ◽

Stem Bark ◽

Oedema Formation ◽

Medicinal Value ◽

Anti Inflammatory ◽

Inflammatory Effect

The plant Piper chaba Hunter (Piperaceae), a climbing glabrous shrub grows in plenty in southern Bangladesh. Popularly known as 'Choi' it is used as spices and believed to have medicinal value in a wide variety of disease conditions including arthritis, asthma, bronchitis and piles. In the present study, effect of methanol extract of Piper chaba stem bark on acute inflammation has been reported. The anti-inflammatory effect was studied in rats by injecting 0.1ml of 1% carrageenan suspension into the planter surface, where oedema of the rat's hind paw was used as an index of acute inflammation. Methanol extract of Piper chaba stem bark given orally 1 hour before injection at doses of 125 and 250 mg/kg body weight, produced significant (p< 0.05) anti inflammatory effect compared to control and the percentage of inhibition of oedema formation was 33% and 35% respectively, which however was less compared to aspirin (46%) and hydrocortisone (56%). The result suggest that in case of acute inflammation, Piper chaba stem bark possess mild to moderate anti inflammatory effect compared to that of aspirin and hydrocortisone.DOI: http://dx.doi.org/10.3329/fmcj.v7i1.10294Faridpur Med. Coll. J. 2012;7(1): 26-28

Download Full-text

Effects of Methanol Extract of Piper chaba Stem Bark on Chronic Inflamation in Rats

Ibrahim Medical College Journal ◽

10.3329/imcj.v2i2.2934 ◽

1970 ◽

Vol 2 (2) ◽

pp. 37-39 ◽

Cited By ~ 2

Author(s):

Fouzia Begum ◽

Kamal Uddin ◽

Syeeda Sultana ◽

Abul Hasnat Ferdous ◽

Zinnat Ara Begum

Keyword(s):

Chronic Inflammation ◽

Acute Inflammation ◽

Methanol Extract ◽

Stem Bark ◽

Granuloma Formation ◽

Medicinal Value ◽

Cotton Pellet ◽

Anti Inflammatory ◽

Implantation Method ◽

Inflammatory Effect

Piper chaba Hunter (Piperaceae), a climbing glabrous shrub grows in plenty in southern Bangladesh. Popularly known as ‘Choi' it is used as spices and believed to have medicinal value in a wide variety of disease conditions including arthritis, asthma, bronchitis and piles. Earlier studies on methanol extract of Piper chaba stem bark have reported anti-inflammatory activities against acute inflammation. In the present study, effect of methanol extract of Piper chaba stem bark on chronic inflammation has been reported. The anti-inflammatory effect was studied in rats using cotton pellet implantation method, where granuloma formation was used as an index of chronic inflammation. Methanol extracts of Piper chaba stem bark given orally for 14 days daily at doses of 125 and 250 mg/kg body weight produced statistically significant (p < 0.05 and p < 0.01) anti-inflammatory effect compared to control. The percent inhibition of granuloma formation was 25% and 28% respectively, which however was less compared to aspirin (41%) and hydrocortisone (58%). The results suggest that in case of chronic inflammation, Piper chaba stem bark possess mild to moderate anti-inflammatory effect compared to that of aspirin and hydrocortisone. Ibrahim Med. Coll. J. 2008; 2(2): 37-39 Key Words: Rats, chronic inflammation, Piper chaba, aspirin, hydrocortisone, choi doi: 10.3329/imcj.v2i2.2934

Download Full-text

In vitro anti-urease, antioxidant, anticholinesterase, cytotoxic and in vivo anti-inflammatory potential of Satureja cuneifolia Ten.

Notulae Scientia Biologicae ◽

10.15835/nsb12210687 ◽

2020 ◽

Vol 12 (2) ◽

pp. 222-232

Author(s):

Turgut TASKIN ◽

Murat DOGAN ◽

Muhammet E. CAM ◽

Talip SAHIN ◽

Ismail SENKARDES

Keyword(s):

Methanol Extract ◽

Radical Scavenging ◽

Inflammatory Activity ◽

Cytotoxic Activities ◽

Methanol Extracts ◽

Aerial Parts ◽

Direct Methanol ◽

Anti Inflammatory

Satureja cuneifolia Ten. (wild savoury) belongs to the Lamiaceae family and is used to produce essential oil and aromatic water. This plant is also used as a condiment and herbal tea due to its stimulating, tonic and carminative effects. The in vitro antioxidant, anti-urease, anticholinesterase and cytotoxic activities of the different extracts from the plant’s aerial parts were examined. Besides, the in vivo anti-inflammatory activities of the fraction and direct methanol extracts were determined comparatively. In the current study, fraction methanol extract exhibited the strongest ABTS (52.34 mM trolox/mg extract) radical scavenging and ferric reduction (17.22 mM Fe2+/mg extract) activity. It was also found that the fraction methanol extract had stronger anti-urease (12.52%) and anticholinesterase (69.02%) activity than other extracts. The XTT results showed that fraction methanol extract had the most cytotoxic activity on MCF-7 cell lines (39.92%). According to the results of in vivo anti-inflammatory activity, it was found that both fraction and direct methanol extracts exhibited close and significant anti-inflammatory activity. The fact that methanol extracts have significant biological activity suggests that these may be used as a natural source in the future.

Download Full-text

In vitro Anti-inflammatory Effect of Carthamus lanatus L.

Zeitschrift für Naturforschung C ◽

10.1515/znc-2003-11-1215 ◽

2003 ◽

Vol 58 (11-12) ◽

pp. 830-832 ◽

Cited By ~ 5

Author(s):

Saima Jalil ◽

Bozhanka Mikhova ◽

Rilka Taskova ◽

Maya Mitova ◽

Helmut Duddeck ◽

...

Keyword(s):

Inflammatory Activity ◽

Human Neutrophils ◽

Inhibitory Effects ◽

Aerial Parts ◽

Anti Inflammatory ◽

Inflammatory Effect

Abstract The anti-inflammatory activity of four total extracts, their fractions and two main constituents (α-bisabolol β-ᴅ-fucopyranoside and luteolin 7-O-glucoside) of Carthamus lanatus L. aerial parts, were assessed in vitro by determining the inhibitory effects on induced human neutrophils. The dichloromethane extract and its water-alcoholic part exhibited the most significant inhibitory effects.

Download Full-text