scholarly journals Effects of Methanol Extract of Piper chaba Stem Bark on Acute Inflammation in rats

2012 ◽  
Vol 7 (1) ◽  
pp. 26-28
Author(s):  
F Begum ◽  
ZA Begum ◽  
MR Uddin ◽  
AHMZ Haider ◽  
RC Barman
Keyword(s):  
Body Weight ◽  
Acute Inflammation ◽  
Methanol Extract ◽  
Stem Bark ◽  
Oedema Formation ◽  
Medicinal Value ◽  
Anti Inflammatory ◽  
Inflammatory Effect

The plant Piper chaba Hunter (Piperaceae), a climbing glabrous shrub grows in plenty in southern Bangladesh. Popularly known as 'Choi' it is used as spices and believed to have medicinal value in a wide variety of disease conditions including arthritis, asthma, bronchitis and piles. In the present study, effect of methanol extract of Piper chaba stem bark on acute inflammation has been reported. The anti-inflammatory effect was studied in rats by injecting 0.1ml of 1% carrageenan suspension into the planter surface, where oedema of the rat's hind paw was used as an index of acute inflammation. Methanol extract of Piper chaba stem bark given orally 1 hour before injection at doses of 125 and 250 mg/kg body weight, produced significant (p< 0.05) anti inflammatory effect compared to control and the percentage of inhibition of oedema formation was 33% and 35% respectively, which however was less compared to aspirin (46%) and hydrocortisone (56%). The result suggest that in case of acute inflammation, Piper chaba stem bark possess mild to moderate anti inflammatory effect compared to that of aspirin and hydrocortisone.DOI: http://dx.doi.org/10.3329/fmcj.v7i1.10294Faridpur Med. Coll. J. 2012;7(1): 26-28

scholarly journals Effects of Methanol Extract of Piper chaba Stem Bark on Chronic Inflamation in Rats

1970 ◽  
Vol 2 (2) ◽  
pp. 37-39 ◽  
Author(s):  
Fouzia Begum ◽  
Kamal Uddin ◽  
Syeeda Sultana ◽  
Abul Hasnat Ferdous ◽  
Zinnat Ara Begum
Keyword(s):  
Chronic Inflammation ◽  
Acute Inflammation ◽  
Methanol Extract ◽  
Stem Bark ◽  
Granuloma Formation ◽  
Medicinal Value ◽  
Cotton Pellet ◽  
Anti Inflammatory ◽  
Implantation Method ◽  
Inflammatory Effect

Piper chaba Hunter (Piperaceae), a climbing glabrous shrub grows in plenty in southern Bangladesh. Popularly known as ‘Choi' it is used as spices and believed to have medicinal value in a wide variety of disease conditions including arthritis, asthma, bronchitis and piles. Earlier studies on methanol extract of Piper chaba stem bark have reported anti-inflammatory activities against acute inflammation. In the present study, effect of methanol extract of Piper chaba stem bark on chronic inflammation has been reported. The anti-inflammatory effect was studied in rats using cotton pellet implantation method, where granuloma formation was used as an index of chronic inflammation. Methanol extracts of Piper chaba stem bark given orally for 14 days daily at doses of 125 and 250 mg/kg body weight produced statistically significant (p < 0.05 and p < 0.01) anti-inflammatory effect compared to control. The percent inhibition of granuloma formation was 25% and 28% respectively, which however was less compared to aspirin (41%) and hydrocortisone (58%). The results suggest that in case of chronic inflammation, Piper chaba stem bark possess mild to moderate anti-inflammatory effect compared to that of aspirin and hydrocortisone. Ibrahim Med. Coll. J. 2008; 2(2): 37-39 Key Words: Rats, chronic inflammation, Piper chaba, aspirin, hydrocortisone, choi   doi: 10.3329/imcj.v2i2.2934

scholarly journals Pharmacognostic Screening and Anti-inflammatory Investigation of the Methanol extract of stem bark of Blighia unijugata Baker (Sapindaceae)

2020 ◽  
Vol 10 (4) ◽  
pp. 146-152
Author(s):  
Felix N. Osuala ◽  
Uchenna E. Odoh ◽  
VictoriaC. Onuigbo ◽  
Sylvester C. Ohadoma
Keyword(s):  
Methanol Extract ◽  
Lethal Dose ◽  
Numerical Data ◽  
Stem Bark ◽  
Water Soluble ◽  
Herbal Remedies ◽  
Albino Rats ◽  
Standard Drug ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Introduction: Blighia unijugata is a potent medicinal plant extensively employed in traditional herbal remedies for the treatment of various diseases such as fever, fertility, inflammation, hypertension, migraine and treatment of infections due to microorganisms. Aim: This work, investigates the pharmacognostic screening and anti-inflammatory activities of the methanol extract of B. unijugata stem bark. Method: Collection, drying, pulverization, and methanol extraction of the stem bark were done accordingly. The screening of phytochemical constituents and Pharmacognostic numerical data were carried out. The chromatographic analysis was carried out using TLC. The acute toxicity was determined using Lorke’s method. Methanol extract was investigated for anti-inflammatory effect in albino rats using egg-induced hind paw oedema at doses of 200, 400 and 600mg/kg body weight respectively. Result: The macroscopical investigation showed the stem bark outer layer is greyish and, the inner layer is pale reddish brown, disagreeable odour, bitter, Microscopical screening revealed the presence of starch grains, trichomes and sclerenchyma cells. Chemomicroscopic result revealed the presence of lignin, starch, calcium oxalate, cellulose, stone cells. Qualitative phytochemical screening of the powdered bark showed the presence of saponins, steroids, tannins, and resins. Numerical data: moisture content/ weight loss on drying gave value of 7.7%, percentage yield of 6.7%, alcoholic soluble extractive 5.6%, water soluble extractive 6.3%, total ash of 7.1%,  acid insoluble ash 0.57% and water soluble ash 4.56%. The chromatographic screening results were close compared with the standard drug. Toxicity test established the lethal dose of greater than 5000mg/kg. There was a significant inhibition of the edema p>0.05, the presence of various bioactive constituents may have contributed to the anti-inflammatory properties of the plant extract. Conclusion: The result of this study confirms that the barks of B. unijugata have anti-inflammatory effect and justifies the use as traditional treatment of inflammation and pain. Keywords: Blighia unijugata, Inflammation, Inflammatory agents, Anti inflammatory

scholarly journals Anti-inflammatory effect of the methanol extract from Anthocephalus cadamba stem bark in animal models

2010 ◽  
Vol 1 (1) ◽  
pp. 6 ◽  
Author(s):  
Kodangala Subraya Chandrashekar ◽  
Kodangala Subraya Prasanna ◽  
Borthakur Abinash
Keyword(s):  
Methanol Extract ◽  
Stem Bark ◽  
Cellular Migration ◽  
Inflammatory Activity ◽  
Traditional Use ◽  
Aerial Parts ◽  
Anti Inflammatory ◽  
Design And Methods ◽  
Anthocephalus Cadamba ◽  
Inflammatory Effect

Background:<em> Anthocephalus cadamba </em>(Rebox)Miq. (Rubiaceae) is widely distributed throughout the greater part of India, especially at low levels in wet place. Traditionally the bark is used as tonic, febrifuge and to reduce the pain and inflammation. The anti-inflammatory effect of methanol extract obtained from <span style="font-style: italic;"> Anthocephalus cadamba </span> aerial parts, MEAC, were investigated in this study. Design and methods: The effects of MEAC on the acute and chronic phases of inflammation were studied in carrageenan, dextran and mediators (histamine and serotonin) induced paw edema and cotton pallet-induced granuloma, respectively. The anti-edema effect of MEAC was compared with 10 mg/kg of indomethacin orally. Results: The results suggested that MEAC possess potent anti-inflammatory activity. The acute inflammatory model showed that all the doses of MEAC effectively suppressed the edema produced by histamine, so it may be suggested that its anti-inflammatory activity is possibly backed by its antihistaminic activity. In chronic inflammatory model the effect may be due to the cellular migration to injured sites and accumulation of collagen and mucopolysaccharide. Conclusions: On the basis of these findings, it may be inferred that <span style="font-style: italic;"> Anthocephalus cadamba </span> is an anti-inflammatory agent and the results are in agreement with its traditional use.

Anti-inflammatory effects of aqueous extract of Mangifera indica in Wistar rats

2015 ◽  
Vol 26 (3) ◽  
Author(s):  
Oluwafemi Gabriel Oluwole ◽  
Celestine Esume
Keyword(s):  
Acetylsalicylic Acid ◽  
Aqueous Extract ◽  
Acute Inflammation ◽  
Mangifera Indica ◽  
Preliminary Investigation ◽  
Stem Bark ◽  
Significant Inhibition ◽  
Oedema Formation ◽  
Anti Inflammatory ◽  
The Right

AbstractRecent studies in standard laboratories have indicated that a typical mango stem bark aqueous extract (This study investigated the anti-inflammatory effects of the aqueous extract ofRats (n=5) were treated orally with MI (50, 100 and 200 mg/kg), acetylsalicylic acid (100 mg/kg) or distilled water (3 mL). Thirty minutes later, acute inflammation was induced with a sub-plantar injection of 0.1 mL of 1% carrageenin solution into the right hind paw of the rats. The paw oedema sizes were measured with the aid of a Vernier calliper over a period of 3 hours.The aqueous extract of MI (50–200 mg/kg, p.o.) produced a dose-dependent and significant inhibition of the acute inflammation induced by the carrageenin in rats when compared with controls. The percentage inhibition of oedema formation produced by MI (200 mg/kg, p.o.) was similar to that elicited by acetylsalicylic acid (100 mg/kg, p.o.).The results of this preliminary investigation suggest that MI contains active compounds with an anti-inflammatory activity. However, more detailed studies using additional models are necessary to further characterise the effects of MI in inflammatory disorders.

scholarly journals Effect of Dichloromethane-Methanol Extract and Tomentin Obtained from Sphaeralcea angustifolia Cell Suspensions in a Model of Kaolin/Carrageenan-Induced Arthritis

2017 ◽  
Vol 4 (01) ◽  
pp. e35-e42 ◽  
Author(s):  
María del Pilar Nicasio-Torres ◽  
Jade Serrano-Román ◽  
Juanita Pérez-Hernández ◽  
Enrique Jiménez-Ferrer ◽  
Maribel Herrera-Ruiz
Keyword(s):  
Body Weight ◽  
Cell Suspension ◽  
Inflammatory Cytokines ◽  
Methanol Extract ◽  
Wild Plants ◽  
Interleukin 4 ◽  
Necrosis Factor Alpha ◽  
Arthritis Model ◽  
Anti Inflammatory ◽  
Inflammatory Effect

AbstractCell suspension cultures from Sphaeralcea angustifolia produce compounds with anti-inflammatory activity, including scopoletin, tomentin, and sphaeralcic acid. Antiarthritic activity of scopoletin isolated from S. angustifolia and Erycibe obtusifolia wild plants was demonstrated in a complete Freud’s adjuvant-induced arthritis model. In this project, the dichloromethane-methanol extract and tomentin isolated from S. angustifolia cell suspension were evaluated in mice using a kaolin/carrageenan-induced arthritis model. After 9 days of treatment, the anti-inflammatory effect of S. angustifolia dichloromethane-methanol extract was 72% at a dose of 100 mg/kg, a recovery of 55% was observed with methotrexate (5.0 mg/kg), and the anti-inflammatory effect for tomentin was dose-dependent with a median effective dose of 10.32 mg/kg. The concentration of pro-inflammatory cytokines interleukin-1β and tumor necrosis factor alpha in the left joint of groups treated with S. angustifolia dichloromethane-methanol extract and tomentin at different doses was significantly lower than that detected in the group damaged with kaolin/carrageenan, and the concentration of interleukin-10 and interleukin-4 (anti-inflammatory cytokines) was significantly higher. The drop in mean body weight of mice treated with methotrexate (−4.34±0.47 g) was higher in comparison with the kaolin/carrageenan group (−2.40±0.29 g). On the other hand, the mean body weight of mice treated with S. angustifolia dichloromethane-methanol extract (−1.19±0.17 g) was similar, and mice treated with tomentin (0.19±0.11 g) was lower. The dichloromethane-methanol extract and tomentin isolated from S. angustifolia diminished joint edema induced by kaolin/carrageenan, possibly by acting as an immunomodulatory of the inflammatory response.

Reduction of paw edema and liver oxidative stress in carrageenan-induced acute inflammation by Lobaria pulmonaria and Parmelia caperata, lichen species, in mice

2019 ◽  
pp. 1-9
Author(s):  
Samira Salem ◽  
Essaid Leghouchi ◽  
Rachid Soulimani ◽  
Jaouad Bouayed
Keyword(s):  
Time Course ◽  
Acute Inflammation ◽  
Lichen Species ◽  
Paw Edema ◽  
Sod Activity ◽  
Edema Formation ◽  
Lobaria Pulmonaria ◽  
Anti Inflammatory ◽  
Endogenous Antioxidant ◽  
Inflammatory Effect

Abstract. Paw edema volume reduction is a useful marker in determining the anti-inflammatory effect of drugs and plant extracts in carrageenan-induced acute inflammation. In this study, the anti-inflammatory effect of Lobaria pulmonaria (LP) and Parmelia caperata (PC), two lichen species, was examined in carrageenan-induced mouse paw edema test. Compared to the controls in carrageenan-induced inflammation (n = 5/group), our results showed that pretreatment by single oral doses with PC extract (50–500 mg/kg) gives better results than LP extract (50–500 mg/kg) in terms of anti-edematous activity, as after 4 h of carrageenan subplantar injection, paw edema formation was inhibited at 82–99% by PC while at 35–49% by LP. The higher anti-inflammatory effect of PC, at all doses, was also observed on the time-course of carrageenan-induced paw edema, displaying profile closely similar to that obtained with diclofenac (25 mg/kg), an anti-inflammatory drug reference (all p < 0.001). Both LP and PC, at all doses, significantly ameliorated liver catalase (CAT) activity (all p < 0.05). However, superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity and glutathione (GSH) levels were found increased in liver of PC- compared to LP-carrageenan-injected mice. Our findings demonstrated on one hand higher preventive effects of PC compared to LP in a mouse carrageenan-induced inflammatory model and suggested, on the other hand, that anti-inflammatory effects elicited by the two lichens were closely associated with the amelioration in the endogenous antioxidant status of liver.

Anti-inflammatory effect of Mitragyna speciosa crude methanol extract on the guinea pig ileum

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
WM Shaik Mossadeq ◽  
K Syamimi ◽  
MP Azyyati ◽  
ZA Zakaria ◽  
AK Arifah ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Methanol Extract ◽  
Guinea Pig Ileum ◽  
Mitragyna Speciosa ◽  
Anti Inflammatory ◽  
Crude Methanol Extract ◽  
Inflammatory Effect

Anti-inflammatory Effect of Allium hookeri Root Methanol Extract in LPS-induced RAW264.7 Cells

2012 ◽  
Vol 41 (11) ◽  
pp. 1645-1648 ◽  
Author(s):  
Chang-Hyun Kim ◽  
Mi-Ai Lee ◽  
Tae-Woon Kim ◽  
Ja Young Jang ◽  
Hyun Ju Kim
Keyword(s):  
Methanol Extract ◽  
Raw264.7 Cells ◽  
Allium Hookeri ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals Anti-inflammatory effect of Barringtonia angusta methanol extract is mediated by targeting of Src in the NF-κB signalling pathway

2021 ◽  
Vol 59 (1) ◽  
pp. 799-810
Author(s):  
Minkyeong Jo ◽  
Jongsung Lee ◽  
Han Gyung Kim ◽  
Jin Kyeong Kim ◽  
Haeyeop Kim ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Signalling Pathway ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals TAK1/AP-1-Targeted Anti-Inflammatory Effects of Barringtonia augusta Methanol Extract

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 3053
Author(s):  
Anh Thu Ha ◽  
Mi-Yeon Kim ◽  
Jae Youl Cho
Keyword(s):  
Mouse Model ◽  
Methanol Extract ◽  
Folk Medicine ◽  
Activator Protein 1 ◽  
Western Blot Assay ◽  
In Vivo Experiments ◽  
Chemokine Ligand ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Barringtonia augusta methanol extract (Ba-ME) is a folk medicine found in the wetlands of Thailand that acts through an anti-inflammatory mechanism that is not understood fully. Here, we examine how the methanol extract of Barringtonia augusta (B. augusta) can suppress the activator protein 1 (AP-1) signaling pathway and study the activities of Ba-ME in the lipopolysaccharide (LPS)-treated RAW264.7 macrophage cell line and an LPS-induced peritonitis mouse model. Non-toxic concentrations of Ba-ME downregulated the mRNA expression of cytokines, such as cyclooxygenase and chemokine ligand 12, in LPS-stimulated RAW264.7 cells. Transfection experiments with the AP-1-Luc construct, HEK293T cells, and luciferase assays were used to assess whether Ba-ME suppressed the AP-1 functional activation. A Western blot assay confirmed that C-Jun N-terminal kinase is a direct pharmacological target of Ba-ME action. The anti-inflammatory effect of Ba-ME, which functions by β-activated kinase 1 (TAK1) inhibition, was confirmed by using an overexpression strategy and a cellular thermal shift assay. In vivo experiments in a mouse model of LPS-induced peritonitis showed the anti-inflammatory effect of Ba-ME on LPS-stimulated macrophages and acute inflammatory mouse models. We conclude that Ba-ME is a promising anti-inflammatory drug targeting TAK1 in the AP-1 pathway.

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