scholarly journals Antioxidant capacity of lipid- and water-soluble antioxidants in dogs with subclinical myxomatous mitral valve degeneration anaesthetised with propofol or sevoflurane

2020 ◽  
Author(s):  
Katerina Tomsič ◽  
Alenka Nemec Svete ◽  
Ana Nemec ◽  
Aleksandra Domanjko Petrič ◽  
Tatjana Pirman ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mitral Valve ◽  
Antioxidant Capacity ◽  
Early Stage ◽  
Water Soluble ◽  
Oxygen Species ◽  
Dental Procedure ◽  
Blood Samples ◽  
Sampling Times

Abstract Background: Antioxidants located in both the hydrophilic and lipophilic compartments of plasma act as a defence system against reactive oxygen species (ROS). . Excessive production of ROS during anaesthesia affects the antioxidant capacity of plasma and may result in oxidative stress. The aim of this study was to evaluate the antioxidant capacity of lipid- (ACL) and water-soluble (ACW) antioxidants in client-owned dogs diagnosed with periodontal disease and early-stage myxomatous mitral valve degeneration (MMVD) and anaesthetised for a dental procedure with propofol and sevoflurane or with propofol only. Results: Dogs with MMVD were anaesthetised with propofol and sevoflurane (MMVD/PS, n = 8) or with propofol only (MMVD/P, n = 10). Dogs with no evidence of MMVD (PS, n = 12) were anaesthetised with propofol and sevoflurane. Blood samples for determination of ACL and ACW were collected before and 5 minutes, 60 minutes and 6 hours after induction to anaesthesia. In dogs with MMVD and anaesthetised with propofol and sevoflurane, ACL was significantly higher at all sampling times when compared to control dogs. Compared to basal values, only anaesthesia maintained with propofol significantly increased ACL at 60 minutes in dogs with MMVD. In dogs with MMVD and anaesthetised with propofol, ACW increased after induction to anaesthesia and remained elevated up to 6 hours after anaesthesia. Compared to basal values, anaesthesia maintained with sevoflurane significantly increased ACW only at 60 minutes in both dogs with and without MMVD.The only difference between propofol and propofol/sevoflurane anaesthesia in dogs with MMVD was significantly higher ACW at 60 minutes after induction to anaesthesia in the propofol group. Conclusions: Regarding antioxidant capacity, propofol could be a better choice than sevoflurane for anaesthesia of dogs with early-stage MMVD, although further studies are necessary to clarify the advantage of this antioxidant capacity.

scholarly journals Antioxidant capacity of lipid- and water-soluble antioxidants in dogs with subclinical myxomatous mitral valve degeneration anaesthetised with propofol or sevoflurane

2020 ◽  
Author(s):  
Katerina Tomsič ◽  
Alenka Nemec Svete ◽  
Ana Nemec ◽  
Aleksandra Domanjko Petrič ◽  
Tatjana Pirman ◽  
...  
Keyword(s):  
Mitral Valve ◽  
Antioxidant Capacity ◽  
The Body ◽  
Water Soluble ◽  
Oxygen Species ◽  
Dental Procedure ◽  
Blood Samples ◽  
Normal Metabolism ◽  
Sampling Times

Abstract Background Antioxidants located in both the hydrophilic and lipophilic compartments of plasma act as a defence system against reactive oxygen species (ROS), which are continuously generated in the body due to both normal metabolism and disease. Excessive production of ROS during anaesthesia affects the antioxidant capacity of plasma and may result in oxidative stress. The aim of this study was to evaluate the antioxidant capacity of lipid- (ACL) and water-soluble (ACW) antioxidants in client-owned dogs diagnosed with periodontal disease and myxomatous mitral valve degeneration (MMVD) and anaesthetised for a dental procedure with propofol and sevoflurane or with propofol only. Results Dogs with MMVD were anaesthetised with propofol and sevoflurane (MMVD/PS, n = 8) or with propofol only (MMVD/P, n = 10). Dogs with no evidence of MMVD (PS, n = 12) were anaesthetised with propofol and sevoflurane. Blood samples for determination of ACL and ACW were collected before and 5 minutes, 60 minutes and 6 hours after induction to anaesthesia. In dogs with MMVD and anaesthetised with propofol and sevoflurane, ACL was significantly higher at all sampling times when compared to control dogs. In dogs with MMVD and anaesthetised with propofol, ACW increased after induction to anaesthesia and remained elevated up to 6 hours after anaesthesia. The only difference between propofol and propofol/sevoflurane anaesthesia in dogs with MMVD was significantly higher ACW at 60 minutes after induction to anaesthesia in the propofol group. Conclusions Compared to basal values, anaesthesia maintained with either propofol or sevoflurane significantly increased ACW in both dogs with and without MMVD. In dogs with MMVD, ACW was significantly higher in the propofol group. Furthermore, only anaesthesia with propofol significantly increased ACL in dogs with MMVD. These results suggest that regarding antioxidant capacity, propofol might be a better choice than sevoflurane for anaesthesia in dogs with MMVD.

scholarly journals Effect of an extract of Hibiscus sabdariffa L., on oxidative stress induced in Saccharomyces cerevisiae

2020 ◽  
Vol 3 (1) ◽  
pp. 41-46
Author(s):  
Franklin Pacheco Coello ◽  
Corymar Orosco-Vargas ◽  
María Peraza-Marrero ◽  
Ibis Pinto-Catari ◽  
Doralys Ramirez-Azuaje
Keyword(s):  
Oxidative Stress ◽  
Saccharomyces Cerevisiae ◽  
Antioxidant Capacity ◽  
Statistical Difference ◽  
Positive Control ◽  
Antioxidant Effect ◽  
Hibiscus Sabdariffa ◽  
Oxygen Species ◽  
Scientific World

Hibiscus sabdariffa-based infusions or drinks are characterized by their high content of bioactive compounds that provide them with an antioxidant capacity that is of great interest in the scientific world. The research consisted in evaluating the antioxidant effect of an aqueous extract of H. sabdariffa, by inducing Saccharomyces cerevisiae to an oxidative stress due to the presence of hydrogen peroxide. The Folin-Ciocalteu method was used for the determination of total phenols in the extract. To evaluate the antioxidant capacity, the extract was diluted, a concentration of 4.5 106 cells/mL of S. cerevisiae was adjusted, and then aliquots of pure and diluted extract were added. Oxidative stress inhibition was observed in each of the assays with statistical difference, relative to the positive control. These results show that H. sabdariffa has an antioxidant capacity that favors the biological activity of S. cerevisiae, contributing to the decrease of the levels of reactive oxygen species and minimizing oxidative damage.

scholarly journals Influence of sevoflurane or propofol anaesthesia on oxidative stress parameters in dogs with early-stage myxomatous mitral valve degeneration. A preliminary study

2018 ◽  
Vol 68 (1) ◽  
pp. 32-42 ◽  
Author(s):  
Tomsič Katerina ◽  
Nemec Svete Alenka ◽  
Nemec Ana ◽  
Domanjko Petrič Aleksandra ◽  
Vovk Tomaž ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Mitral Valve ◽  
Glutathione Peroxidase ◽  
Early Stage ◽  
Glutathione Peroxidase Activity ◽  
Oxidative Stress Parameters ◽  
Preliminary Study ◽  
Stress Parameters

Abstract The aim of this study was to investigate the effects of total intravenous anaesthesia with propofol and anaesthesia induced with propofol and maintained with sevoflurane on oxidative stress parameters in dogs with early-stage myxomatous mitral valve degeneration (MMVD). Sixteen client-owned dogs with early stage MMVD that required periodontal treatment were included in the study. After induction with propofol, anaesthesia was maintained with propofol (group P) or sevoflurane (group PS). Blood samples for determination of vitamin E, superoxide dismutase, glutathione peroxidase and malondialdehyde were collected before premedication, 5 and 60 minutes and 6 hours after induction to anaesthesia. There were no significant differences between groups in any of the oxidative stress parameters at each sampling time. Compared to basal values, vitamin E concentration decreased significantly during anaesthesia in both groups and glutathione peroxidase activity increased 60 minutes after induction to anaesthesia in PS group. Anaesthesia with propofol or with propofol and sevoflurane did not have any significant impact on oxidative stress parameters in dogs with early stage MMVD. In terms of oxidative stress, both protocols may be equally safely used in dogs with early stage MMVD.

scholarly journals Oxidative Stress, Telomere Shortening, and Apoptosis Associated to Sarcopenia and Frailty in Patients with Multimorbidity

2020 ◽  
Vol 9 (8) ◽  
pp. 2669 ◽  
Author(s):  
Máximo Bernabeu-Wittel ◽  
Raquel Gómez-Díaz ◽  
Álvaro González-Molina ◽  
Sofía Vidal-Serrano ◽  
Jesús Díez-Manglano ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Telomere Length ◽  
Prospective Observational Study ◽  
Oxygen Species ◽  
Oxidative Stress Markers ◽  
Blood Leukocytes ◽  
Telomere Shortening ◽  
Blood Samples ◽  
Stress Markers ◽  
Total Antioxidant

Background: The presence of oxidative stress, telomere shortening, and apoptosis in polypathological patients (PP) with sarcopenia and frailty remains unknown. Methods: Multicentric prospective observational study in order to assess oxidative stress markers (catalase, glutathione reductase (GR), total antioxidant capacity to reactive oxygen species (TAC-ROS), and superoxide dismutase (SOD)), absolute telomere length (aTL), and apoptosis (DNA fragmentation) in peripheral blood samples of a hospital-based population of PP. Associations of these biomarkers to sarcopenia, frailty, functional status, and 12-month mortality were analyzed. Results: Of the 444 recruited patients, 97 (21.8%), 278 (62.6%), and 80 (18%) were sarcopenic, frail, or both, respectively. Oxidative stress markers (lower TAC-ROS and higher SOD) were significantly enhanced and aTL significantly shortened in patients with sarcopenia, frailty or both syndromes. No evidence of apoptosis was detected in blood leukocytes of any of the patients. Both oxidative stress markers (GR, p = 0.04) and telomere shortening (p = 0.001) were associated to death risk and to less survival days. Conclusions: Oxidative stress markers and telomere length were enhanced and shortened, respectively, in blood samples of polypathological patients with sarcopenia and/or frailty. Both were associated to decreased survival. They could be useful in the clinical practice to assess vulnerable populations with multimorbidity and of potential interest as therapeutic targets.

scholarly journals Effect of packing on changes in erythrocyte osmotic fragility and malondialdehyde concentration in donkeys administered with ascorbic acid

2012 ◽  
Vol 79 (1) ◽  
Author(s):  
Folashade Olaifa ◽  
Joseph O. Ayo ◽  
Suleiman F. Ambali ◽  
Peter I. Rekwot
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Osmotic Fragility ◽  
Blood Samples ◽  
Experimental Animals ◽  
Erythrocyte Osmotic Fragility ◽  
Nacl Concentration ◽  
Experimental Subjects ◽  
Apparently Healthy

Experiments were performed with the aim of investigating the effect of packing on erythrocyte osmotic fragility (EOF) and malondialdehyde (MDA) concentration in donkeys, and the effect of ascorbic acid (AA). Twelve apparently healthy donkeys raised under the traditional extensive system served as experimental subjects. Six donkeys administered orally with AA (200 mg/kg) and subjected to packing were used as experimental animals, whilst six others not administered with AA served as controls. Blood samples were collected pre- and post-packing from all the donkeys for the determination of MDA and EOF. At 0.3% Sodium Chloride (NaCl) concentration, the percentage haemolysis was 93.69% ± 2.21% in the control donkeys and the value was significantly (P < 0.05) higher than the value of 71.31% ± 8.33%, recorded in the experimental donkeys. The post-packing MDA concentration obtained in the control donkeys was 39.62 µmol ± 4.16 µmol, and was not significantly different (P > 0.05) from the value of 35.97 µmol ± 2.88 µmol recorded in the experimental donkeys. In conclusion, the increase in haemolysis obtained in the donkeys suggested that packing induced oxidative stress, which was ameliorated by AA administration.

The effects of a water-soluble alpha tetra-substituted zinc phthalocyanine derivative onArthrospira platensis-M2 strain

2018 ◽  
Vol 22 (08) ◽  
pp. 686-692 ◽  
Author(s):  
Armağan Günsel ◽  
Hatice Tunca ◽  
Ahmet T. Bilgiçli ◽  
Ali Doğru ◽  
M. Nilüfer Yaraşir ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Biomass Accumulation ◽  
Arthrospira Platensis ◽  
Water Soluble ◽  
Zinc Phthalocyanine ◽  
Natural Environments ◽  
Oxygen Species ◽  
Sod Activity ◽  
Future Studies

In this study, we have analyzed the effect a newly synthesized water-soluble alpha tetra-substituted zinc phthalocyanine (Pc) compound on superoxide dismutase (SOD), ascorbate peroxidase (APX) and glutathione reductase (GR) activities and biomass accumulation in the Arthrospira platensis-M2 strain to test whether this compound could be used as an algaecide or not. We found that lower concentrations (3 μg mL[Formula: see text] and 6 μg mL[Formula: see text] of Pc compound were not toxic to algae cells, as indicated by enduring biomass accumulation during the study (7 days). Higher Pc concentrations, however, were toxic and inhibited biomass accumulation. This inhibition appeared on the fourth day and persisted during the study. At higher Pc concentrations, SOD activity decreased significantly, but APX and GR activity were not affected. These results may show that Pc applications did not cause the accumulation of reactive oxygen species in Arthrospira platensis-M2 cells. Our result suggests that higher Pc concentrations did not cause oxidative stress but biomass accumulation inhibited, possibly due to some different toxicity mechanism(s), which should be carried out in the future studies. As a result, we may offer use of this compound as a means to keep under control algal populations in natural environments.

PSXV-27 Late-Breaking: Evaluation of antioxidant capacity in receiving beef calves challenged with lipopolysaccharide (LPS)

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 381-382
Author(s):  
Samantha N Barker ◽  
Treylr Jackson ◽  
John Richeson ◽  
Jeff A Carroll ◽  
Nicole C Burdick-Sanchez ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Repeated Measures ◽  
Colorimetric Assay ◽  
Post Challenge ◽  
Antioxidant Power ◽  
Blood Samples ◽  
Endotoxin Challenge ◽  
Beef Calves ◽  
Lps Challenge

Abstract The objective of this study was to evaluate antioxidant capacity in plasma of beef calves challenged with LPS. Following an initial feeding period of 40 d, steers (n = 32; 379 kg ± 30.7) were transported to the Livestock Issues Research Unit’s Bovine Immunology Research and Development Complex and challenged intravenously with LPS (0.25 µg/kg BW) on d 41. Blood samples were collected via jugular catheter at -2, 0, 2, 4, 6, 8, 10, 12, 18, 24, 36 and 48 h relative to the LPS challenge at 0 h. Blood samples were processed to isolate plasma for indicators of oxidative stress with a colorimetric assay to determine ferric reducing antioxidant power (FRAP) values via concentration of ferrous iron (µM). Data were analyzed as repeated measures using the GLIMMIX procedure of SAS. Antioxidant values did vary with time (P &lt; 0.001) being greater (P &lt; 0.05) at -2, 0, 2, 36, and 48 h. Antioxidant capacity was reduced at 6 and 8 h (P &lt; 0.05), with the least FRAP value observed at 8 h post-challenge. Antioxidant capacity increased (P &lt; 0.05) again at 10 h, showing similar (P &gt; 0.05) concentrations to those observed at 4 h. By 24 h post-challenge, plasma FRAP values increased (P &lt; 0.05) similar to initial values at -2, 0, and 2 h. It can be inferred that oxidative stress contributes to reduced antioxidant capacity, ultimately interfering with animal growth and productivity. While these values reflect the oxidative stress response to an acute endotoxin challenge, and a subsequent recovery returning to homeostasis within 24 to 48 h, they may also correlate with other physiological and immunological indicators associated with an acute endotoxin challenge.

Changes in the total amount of water-soluble antioxidants in the blood serum of Duroc boars depending on the duration of their fattening

2020 ◽  
Vol 1 (12) ◽  
pp. 112-118
Author(s):  
O. A. Voronina ◽  
◽  
A. A. Savina ◽  
N. S. Kolesnik ◽  
N. V. Bogolyubova ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Blood Serum ◽  
Water Soluble ◽  
Laboratory Studies ◽  
Blood Samples ◽  
Amperometric Method ◽  
Significant Difference ◽  
Total Antioxidant ◽  
First Time

The influence of fattening conditions on the digestive and metabolic processes in animals is actively studied to increase the production of animal food. The aim of the study was to estimate the total amount of water-soluble antioxidants in the blood serum of Duroc boars depending on the duration of fattening. Experiments were conducted on 102 animals to confirm the results of laboratory studies, as well as determination of the total antioxidant activity of pig blood serum by amperometric method. For the first time, a significant difference in antioxidant activity was found in blood samples of Duroc boars with the duration of their fattening from 81 to 101 days.

scholarly journals An Immunohistochemical Study of the Increase in Antioxidant Capacity of Corneal Epithelial Cells by Molecular Hydrogen, Leading to the Suppression of Alkali-Induced Oxidative Stress

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Cestmir Cejka ◽  
Jan Kossl ◽  
Vladimir Holan ◽  
John H. Zhang ◽  
Jitka Cejkova
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Antioxidant Capacity ◽  
Molecular Hydrogen ◽  
Reactive Oxygen ◽  
Corneal Neovascularization ◽  
Scar Formation ◽  
Oxygen Species ◽  
Buffer Solution ◽  
Corneal Epithelial

Corneal alkali burns are potentially blinding injuries. Alkali induces oxidative stress in corneas followed by excessive corneal inflammation, neovascularization, and untransparent scar formation. Molecular hydrogen (H2), a potent reactive oxygen species (ROS) scavenger, suppresses oxidative stress and enables corneal healing when applied on the corneal surface. The purpose of this study was to examine whether the H2 pretreatment of healthy corneas evokes a protective effect against corneal alkali-induced oxidative stress. Rabbit eyes were pretreated with a H2 solution or buffer solution, by drops onto the ocular surface, and the corneas were then burned with 0.25 M NaOH. The results obtained with immunohistochemistry and pachymetry showed that in the corneas of H2-pretreated eyes, slight oxidative stress appeared followed by an increased expression of antioxidant enzymes. When these corneas were postburned with alkali, the alkali-induced oxidative stress was suppressed. This was in contrast to postburned buffer-pretreated corneas, where the oxidative stress was strong. These corneas healed with scar formation and neovascularization, whereas corneas of H2-pretreated eyes healed with restoration of transparency in the majority of cases. Corneal neovascularization was strongly suppressed. Our results suggest that the corneal alkali-induced oxidative stress was reduced via the increased antioxidant capacity of corneal cells against reactive oxygen species (ROS). It is further suggested that the ability of H2 to induce the increase in antioxidant cell capacity is important for eye protection against various diseases or external influences associated with ROS production.

scholarly journals Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress

Antioxidants ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 16 ◽  
Author(s):  
Flávia Póvoa da Costa ◽  
Bruna Puty ◽  
Lygia S. Nogueira ◽  
Geovanni Pereira Mitre ◽  
Sávio Monteiro dos Santos ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Cell Death ◽  
Cell Viability ◽  
Antioxidant Capacity ◽  
Periodontal Ligament ◽  
Cellular Metabolism ◽  
Mitochondrial Activity ◽  
Oxygen Species ◽  
Atp Production

Piceatannol is a resveratrol metabolite that is considered a potent antioxidant and cytoprotector because of its high capacity to chelate/sequester reactive oxygen species. In pathogenesis of periodontal diseases, the imbalance of reactive oxygen species is closely related to the disorder in the cells and may cause changes in cellular metabolism and mitochondrial activity, which is implicated in oxidative stress status or even in cell death. In this way, this study aimed to evaluate piceatannol as cytoprotector in culture of human periodontal ligament fibroblasts through in vitro analyses of cell viability and oxidative stress parameters after oxidative stress induced as an injury simulator. Fibroblasts were seeded and divided into the following study groups: control, vehicle, control piceatannol, H2O2 exposure, and H2O2 exposure combined with the maintenance in piceatannol ranging from 0.1 to 20 μM. The parameters analyzed following exposure were cell viability by trypan blue exclusion test, general metabolism status by the 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) method, mitochondrial activity through the ATP production, total antioxidant capacity, and reduced gluthatione. Piceatannol was shown to be cytoprotective due the maintenance of cell viability between 1 and 10 μM even in the presence of H2O2. In a concentration of 0.1 μM piceatannol decreased significantly cell viability but increased cellular metabolism and antioxidant capacity of the fibroblasts. On the other hand, the fibroblasts treated with piceatannol at 1 μM presented low metabolism and antioxidant capacity. However, piceatannol did not protect cells from mitochondrial damage as measured by ATP production. In summary, piceatannol is a potent antioxidant in low concentrations with cytoprotective capacity, but it does not prevent all damage caused by hydrogen peroxide.

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