Screening of aqueous and alcoholic extracts of some Indian medicinal plants for antibacterial activity

2006 ◽  
Vol 68 (6) ◽  
pp. 835 ◽  
Author(s):  
Sumitra Chanda ◽  
Jigna Parekh
2007 ◽  
Vol 61 (3) ◽  
pp. 313-317 ◽  
Author(s):  
Daljit Singh Arora ◽  
Gurinder Jeet Kaur

Author(s):  
Sumathy Rengarajan ◽  
Vijayalakshmi Melanathuru ◽  
Deecaraman Munuswamy ◽  
Sankaranarayanan Sundaram ◽  
Saravanan Thiruverkadu Selvaraj

ABSTRACTObjective: The present study was to evaluate the in vitro antibacterial activity, and thin-layer chromatography (TLC) studies from the petals of fourdifferent Indian medicinal plants (Punica granatum, Hibiscus rosa-sinensis, Cassia auriculata, and Moringa oleifera).Methods: The phytochemical screening of the methanol extract of petals of four different Indian medicinal plants was performed using standardprocedures. The antimicrobial activity was tested against various test organisms using the agar disc diffusion method.Results: The preliminary phytochemical screening for petals of four different medicinal plants revealed the presence of flavonoids, alkaloids, tannins,and saponins. From the above study, the results indicated that the methanol extract of M. oleifera petals showed the highest antimicrobial activityagainst Staphylococcus aureus and Bacillus subtilis with zone of inhibition 17.93 and 23.40, respectively, at the concentration of 20 µl/ml and alsoshowed the maximum inhibitory activity at the highest concentration (20 µl/ml) than the lowest concentration (5 µl/ml) against Gram-negativebacteria such as Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, and Gram-positive B. subtilis and S. aureus. TLC studies of methanolextracts of petals of Indian medicinal plants revealed the presence of different phytoconstituents as evidenced by separated compounds with differentRf values.Conclusion: The results obtained in the present study indicate that the petals of four different Indian medicinal plants showed the highest antibacterialactivity and can be used as an antibacterial agent against bacterial diseases.Keywords: Phytochemicals, Antibacterial activity, Thin-layer chromatography.


2015 ◽  
Vol 8 (3/4) ◽  
pp. 262 ◽  
Author(s):  
T. Lakshmipriya ◽  
T. Meenambal ◽  
R. Lavanyasri ◽  
S. Nandhini ◽  
N. Pavithra ◽  
...  

2020 ◽  
Vol 18 ◽  
Author(s):  
Mulugeta Mulat ◽  
Fazlurrahman Khan ◽  
Archana Pandita

Background: Medicinal plants have been used for treatments of various health ailments and the practices as a remedial back to thousands of years. Currently, plant-derived compounds used as alternative ways of treatment for multidrug-resistant pathogens. Objective: In the present study, various parts of six medical plants such as Solanum nigrum, Azadirachta indica, Vitex negundo, Mentha arvensis, Gloriosa superba, and Ocimum sanctum were extracted for obtaining biological active constituents. Methods: Soxhlet method of extraction was used for obtaining crude extracts. Agar disc diffusion and 96-well plate spectroscopic reading were used to detect the extract’s antibacterial and antibiofilm properties. Results: The obtained extracts were tested for antimicrobial and antibiofilm properties at 25 mg/mL concentrations. Maximum antibacterial activity was observed in O. sanctum chloroform extract (TUCE) against Staphylococcus aureus (24.33±1.52 mm), S. nigrum acetone extract (MAAC) against Salmonella Typhimurium (12.6 ± 1.5 mm) and Pseudomonas aeruginosa (15.0 ±2.0 mm). Only TUCE exhibited antibacterial activity at least a minimum inhibitory concentration of 0.781 mg/mL. Better antibiofilm activities were also exhibited by petroleum extracts of G. superba (KAPE) and S. nigrum (MAPE) against Escherichia coli, S. Typhimurium, P. aeruginosa and S. aureus. Moreover, S. nigrum acetone extract (MAAC) and O. sanctum chloroform extract (TUCE) were showed anti-swarming activity with a reduction of motility 56.3% against P. aeruginosa and 37.2% against S. aureus. MAAC also inhibits Las A activity (63.3% reduction) in P. aeruginosa. Conclusion: Extracts of TUCE, MAAC, MAPE, and KAPE were exhibited antibacterial and antibiofilm properties against the Gram-positive and Gram-negative pathogenic bacteria. GCMS identified chemical constituents are responsible for being biologically active.


Sign in / Sign up

Export Citation Format

Share Document