scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL ACTIVITY OF INDIAN MEDICINAL PLANTS AGAINST HUMAN PATHOGENS.

2017 ◽  
Vol 5 (8) ◽  
pp. 1459-1464
Author(s):  
MargiH Patel ◽  
◽  
FaridaP Minocheherhomji ◽  
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Phytochemical Screening ◽  
Human Pathogens ◽  
Indian Medicinal Plants

A COMPARATIVE STUDY OF IN VITRO ANTIMICROBIAL ACTIVITY AND TLC STUDIES OF PETALS OF SELECTED INDIAN MEDICINAL PLANTS

2016 ◽  
Vol 9 (5) ◽  
pp. 259 ◽  
Author(s):  
Sumathy Rengarajan ◽  
Vijayalakshmi Melanathuru ◽  
Deecaraman Munuswamy ◽  
Sankaranarayanan Sundaram ◽  
Saravanan Thiruverkadu Selvaraj
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Methanol Extract ◽  
Phytochemical Screening ◽  
Layer Chromatography ◽  
Indian Medicinal Plants

ABSTRACTObjective: The present study was to evaluate the in vitro antibacterial activity, and thin-layer chromatography (TLC) studies from the petals of fourdifferent Indian medicinal plants (Punica granatum, Hibiscus rosa-sinensis, Cassia auriculata, and Moringa oleifera).Methods: The phytochemical screening of the methanol extract of petals of four different Indian medicinal plants was performed using standardprocedures. The antimicrobial activity was tested against various test organisms using the agar disc diffusion method.Results: The preliminary phytochemical screening for petals of four different medicinal plants revealed the presence of flavonoids, alkaloids, tannins,and saponins. From the above study, the results indicated that the methanol extract of M. oleifera petals showed the highest antimicrobial activityagainst Staphylococcus aureus and Bacillus subtilis with zone of inhibition 17.93 and 23.40, respectively, at the concentration of 20 µl/ml and alsoshowed the maximum inhibitory activity at the highest concentration (20 µl/ml) than the lowest concentration (5 µl/ml) against Gram-negativebacteria such as Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, and Gram-positive B. subtilis and S. aureus. TLC studies of methanolextracts of petals of Indian medicinal plants revealed the presence of different phytoconstituents as evidenced by separated compounds with differentRf values.Conclusion: The results obtained in the present study indicate that the petals of four different Indian medicinal plants showed the highest antibacterialactivity and can be used as an antibacterial agent against bacterial diseases.Keywords: Phytochemicals, Antibacterial activity, Thin-layer chromatography.

Antibacterial activity of some Indian medicinal plants

2007 ◽  
Vol 61 (3) ◽  
pp. 313-317 ◽  
Author(s):  
Daljit Singh Arora ◽  
Gurinder Jeet Kaur
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Indian Medicinal Plants

scholarly journals The Antibacterial Activity of Aqueous and Ethanolic Leaf Extracts of Balanites aegyptiaca (l.) Del Plant on Some Selected Clinical Human Pathogens

2020 ◽  
pp. 51-66
Author(s):  
G. C. Ezemokwe ◽  
J. C. Aguiyi ◽  
F. P. Chollom
Keyword(s):  
Antibacterial Activity ◽  
Leaf Extract ◽  
Activity Index ◽  
Phytochemical Screening ◽  
Human Pathogens ◽  
Leaf Extracts ◽  
Balanites Aegyptiaca ◽  
Minimal Inhibitory Concentrations ◽  
Significant Difference ◽  
Zones Of Inhibition

The antibacterial activity of the leaf extract of Balanites aegyptiaca plant was investigated on five selected clinical common human pathogens: Staphylococcus aureus, Streptococcus pneumonia, Salmonella typhimurium, Shigella dysentriae and Escherichia coli, in vitro. The phytochemical screening, susceptibility testing and Minimal Inhibitory Concentrations were determined. Preliminary phytochemical screening of the leaf extracts indicated the presence of alkaloids, cardiac glycosides, flavonoids, glycosides, resins, saponins, steroids, tannins, and carbohydrates in varying concentrations. Ethanolic leaf extract was more effective. The extracts inhibited the growth of isolates with increasing concentrations, ranging from 8-12 mm zones of inhibitions, showing the susceptibility of the test organisms. S. dysentriae had the highest susceptibility for both extracts with 12 mm and 13 mm zones of inhibition for aqueous and ethanolic extracts respectively at the highest concentration of 400 mg/ml. S. aureus showed the least susceptibility with 8 mm for aqueous extract while S. typhimurium showed the least susceptibility for the ethanolic extract with 9mm zones of inhibition. The analysis of variance results on zones of inhibition revealed a significant difference for both extract treatments, concentrations, and interactions between the treatments and concentrations. The Minimal Inhibitory Concentrations determined from the corresponding concentration-response curves showed that S. dysentriae had the least value of 79.433 mg/ml while the aqueous extracts against S. aureus and S. typhimurium were the largest with 125.893 mg/ml value. The activity index and activity were also deduced to measure activity. The antimicrobial activity of leaf extracts of B. aegyptiaca on bacteria of the tested isolates have been established in this study and justify the claims by the traditional healers in its use to treat infectious diseases.

Phytochemical Screening and Antibacterial Activity of Five Malaysian Medicinal Plants

2014 ◽  
Vol 4 (17) ◽  
pp. 2019-2032 ◽  
Author(s):  
Rasha Saad ◽  
Loshini Appalasamy ◽  
Jiyauddin Khan ◽  
Hamid Kazi ◽  
Eddy Yusuf ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Phytochemical Screening

scholarly journals Phytochemical Screening, Antibacterial Activity and Heavy Metal Analysis of Ethnomedicinal Recipes and Their Sources Used Against Infectious Diseases

Plants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 454 ◽  
Author(s):  
Nasir Mahmood ◽  
Ruqia Nazir ◽  
Muslim Khan ◽  
Rashid Iqbal ◽  
Muhammad Adnan ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Respiratory Tract ◽  
Respiratory Tract Infections ◽  
Phytochemical Screening ◽  
Metal Analysis ◽  
Heavy Metal Analysis ◽  
Maximum Quantity ◽  
Tract Infections

Plants are a rich source of secondary metabolites that have been found to have medicinal properties. The present study was conducted to evaluate the phytochemical screening, antibacterial activities and heavy metal analysis of seven medicinal plants i.e., Nigella sativa (seeds), Trigonella foenum-graecum (seeds), Brassica campestris (seeds), Pistacia integerrima (galls), Linum usitatissimum (seeds), Hyssopus officinalis (flowers), Ephedra vulgaris (dry branches) and its two recipes which are used by hakims (Practitioners of local herbal medicines), against different diseases particularly respiratory tract infections. The obtained results revealed that alkaloids (30%) and flavonoids (41%) were in maximum quantity in P. integerrima (galls) while saponins (10.9%) were in maximum quantity in Recipe 1. The antibacterial activity was determined by the agar well disc diffusion method using methanol, ethanol, chloroform and deionized water extracts. Each plant extract was tested against one Gram-positive (Streptococcus pneumonia) and two Gram-negative (Pseudomonas aeruginosa and Klebsiella pneumonia) bacteria. Maximum zones of inhibition in methanol, ethanol, chloroform and aqueous extract were seen in T. foenum-graecum against S. pneumonia (20.06 ± 0.16 mm), B. campestris against S. pneumonia (22.40 ± 0.24 mm), Recipe 2 against K. pneumonia (20.06 ± 0.16 mm) and N. sativa against S. pneumonia (20.23 ± 0.16 mm), respectively. The concentrations of heavy metals were determined by atomic absorption spectrophotometer and showed the existence of high concentration of Iron (Fe), Lead (Pb) and Chromium (Cr). Thus, it has been found that medicinal plants individually as well as their recipes are potentially active against various diseases particularly respiratory tract infections

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