Short-term visual outcomes of Boston keratoprosthesis type i in Saudi Arabia

AbstractInspired by the effectiveness of low-intensity ultrasound on tissue regeneration, we investigated the potential effect of short-term high-intensity ultrasound treatment for acceleration of wound healing in an in vitro wound model and dermal equivalent, both comprising human dermal fibroblasts. Short-term ultrasound of various amplitudes significantly increased the proliferation and migration of fibroblasts and subsequently increased the production of the extracellular matrix components fibronectin and collagen type I, both of which are important for wound healing and are secreted by fibroblasts. In addition, ultrasound treatment increased the contraction of a fibroblast-embedded three-dimensional collagen matrix, and the effect was synergistically increased in the presence of TGF-β. RNA-sequencing and bioinformatics analyses revealed changes in gene expression and p38 and ERK1/2 MAPK pathway activation in the ultrasound-stimulated fibroblasts. Our findings suggest that ultrasound as a mechanical stimulus can activate human dermal fibroblasts. Therefore, the activation of fibroblasts using ultrasound may improve the healing of various types of wounds and increase skin regeneration.

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Errata: Advantages of microscope-integrated intraoperative online optical coherence tomography: usage in Boston keratoprosthesis type I surgery

Journal of Biomedical Optics ◽

10.1117/1.jbo.21.2.029801 ◽

2016 ◽

Vol 21 (2) ◽

pp. 029801 ◽

Cited By ~ 1

Author(s):

Sebastian Siebelmann ◽

Philipp Steven ◽

Deniz Hos ◽

Gereon Hüttmann ◽

Eva Lankenau ◽

...

Keyword(s):

Optical Coherence Tomography ◽

Type I ◽

Optical Coherence ◽

Boston Keratoprosthesis

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Long-term outcomes of Boston keratoprosthesis type I: the Chinese People’s Liberation Army General Hospital experience

British Journal of Ophthalmology ◽

10.1136/bjophthalmol-2019-315617 ◽

2021 ◽

pp. bjophthalmol-2019-315617

Author(s):

Li-Qiang Wang ◽

Teng-Yun Wu ◽

Xiao-Niao Chen ◽

Ze-Quan Xu ◽

Min Yang ◽

...

Keyword(s):

General Hospital ◽

Ocular Surface ◽

Retention Rate ◽

Case Series ◽

Type I ◽

Long Term Outcomes ◽

Boston Keratoprosthesis ◽

People's Liberation Army ◽

People’S Liberation Army

PurposeTo report the long-term outcomes of Boston keratoprosthesis type I (B-KPro type I) implantation in the management of severe ocular surface disorders.MethodsRetrospective case series. Patients who underwent B-KPro type I implantation at the People’s Liberation Army General Hospital were enrolled between March 2011 and September 2019. Data regarding visual acuity (VA), B-KPro type I retention and postoperative complications were recorded and analysed.ResultsA total of 103 eyes of 100 patients who underwent B-KPro type I implantation were included. The main indications were chemical burn (59.2%), ocular trauma (25.2%), herpetic keratitis (11.7%) and autoimmune diseases (3.9%). The percentage of eyes with postoperative VA of 10/200 or better was 82.7% at 6 months, 82.8% at 12 months, 77.9% at 2 years, 72.4% at 3 years, 71.1% at 4 years, 69.4% at 5 years, 58.9% at 6 years, 56.8% at 7 years and 42.9% at 8 years. Preoperatively, 8.7% eyes were diagnosed with new-onset glaucoma. Retroprosthetic membrane formation occurred in 19.4% eye. Corneal melting occurred in 18.4% eyes. Sterile vitritis was diagnosed in 4.9% eyes and infectious endophthalmitis in 2.9% eyes. Retinal detachment occurred in 0.9% eyes.ConclusionsIn a Chinese patient group, B-KPro type I is a viable option for treating severe ocular surface disorders in eyes where conventional keratoplasty would have a poor prognosis, especially in patients with chemical and thermal burns. Improved visual outcomes and high retention rate can be achieved and maintained in most cases.

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The amounts of rat liver cyclic AMP-dependent protein kinase I and II are differentially regulated by diet

Biochemical Journal ◽

10.1042/bj2560447 ◽

1988 ◽

Vol 256 (2) ◽

pp. 447-452 ◽

Cited By ~ 6

Author(s):

R Ekanger ◽

O K Vintermyr ◽

S O Døskeland

Keyword(s):

Protein Kinase ◽

Cyclic Amp ◽

Protein Restriction ◽

Type I ◽

Short Term ◽

Rat Hepatocyte ◽

Dependent Protein Kinase ◽

Key Enzyme ◽

Dependent Protein ◽

Fasted Rats

1. The fluctuations in rat hepatocyte volume and protein content in response to dietary perturbations (starvation, protein restriction, refeeding) were accompanied by corresponding fluctuations in the amount of the regulatory (R) and catalytic (C) subunits of cyclic AMP-dependent protein kinase. Thus the intracellular concentration of this key enzyme was adjusted to be near constant. 2. The adjustment of cellular R was accomplished almost exclusively by regulating cytosolic RI (R subunit of type I kinase). The preferential down-regulation of cytosolic RI in response to starvation/protein restriction indicates that particulate RI and cytosolic as well as particulate RII are more resistant to breakdown during general catabolism in the hepatocyte. 3. The diet-induced fluctuations of kinase subunits were uniformly distributed in all populations of parenchymatous hepatocytes, regardless of their size and density. It is thus possible to isolate hepatocytes with uniformly altered RI/RII ratio from livers of rats with different feeding regimens. 4. The binding of endogenous cyclic AMP to RI and RII was similar in livers with high RI/RII ratio (fed rats) and low RI/RII ratio (fasted rats) as well as in hepatocytes isolated from fasted rats. Under the conditions of the experiment (short-term stimulation by glucagon), therefore, neither the dietary state nor the RI/RII ratio seemed to affect the apparent affinity of the isoreceptors for cyclic AMP. However, RI appeared to show a slightly higher co-operativity of intracellular cyclic AMP binding than did RII in all states.

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