Comparative evaluation of the three different surface treatments - conventional, laser and Nano technology methods in enhancing the surface characteristics of commercially pure titanium discs and their effects on cell adhesion: An in vitro study

This study investigated the surface characteristics of ultrafine-grain commercially pure titanium (UFG CP-Ti) substrates produced by equal channel angular pressing (ECAP), compared with those of coarse-grain commercially pure titanium (CG CP-Ti) and Ti–6Al–4V (Ti-64) substrates. All Ti surfaces were sandblasted and acid-etched (SLA-treated) to produce micro-rough surfaces. Tensile and microhardness tests were carried out to measure the mechanical properties of fabricated samples. Then the surface characteristics of samples including contact angle measurements, surface morphology and in vitro cell response were evaluated after polishing, sandblasting and acid etching procedures. The results showed that after applying four passes of ECAP, the average grain size of microstructure decreased from 25 µm to 170 nm, while the ultimate tensile strength increased from 545 ± 24 MPa to 971 ± 38 MPa. Investigation of surface morphologies carried out by scanning electron microscopy indicated that ECAP-processed substrate exhibits nano-topography compared with CG CP-Ti and Ti-64 substrates after applying SLA process. In addition, the contact angle of SLA-treated CG CP-Ti and UFG CP-Ti substrates decreased from 68.3° to 9.5° and 51.9° to 7.4°, respectively, indicating a significant improvement of surface wettability. The morphologies of MG63 cells cultured on the developed surfaces proved the potential superior osteoblast cell compatibility of the micro-roughened surface made of UFG CP-Ti substrates over CG CP-Ti and Ti-64 substrates.

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Platelet Adhesion on Commercially Pure Titanium Plates in Vitro II. Immunofluorescence Visualization of PDGF-B, TGFβ1, and PPARγ Released from Activated Adherent Platelets

Dentistry Journal ◽

10.3390/dj7040109 ◽

2019 ◽

Vol 7 (4) ◽

pp. 109 ◽

Cited By ~ 2

Author(s):

Tetsuhiro Tsujino ◽

Akira Takahashi ◽

Taisuke Watanabe ◽

Kazushige Isobe ◽

Yutaka Kitamura ◽

...

Keyword(s):

Industrial Development ◽

Platelet Adhesion ◽

Alveolar Bone ◽

Platelet Rich Plasma ◽

Pure Titanium ◽

Commercially Pure Titanium ◽

Commercially Pure ◽

Titanium Surfaces ◽

Cp Ti

Recent progress in the industrial development of dental implants has improved their surface bio-affinity, while clinical implantologists attempt to improve it through coating with various compounds, including platelet-rich plasma (PRP) in clinical settings. However, it is poorly understood how PRP acts on titanium surfaces. To validate this surface modification method and demonstrate how platelet-derived soluble biomolecules released from the activated adherent platelets act on plain, commercially pure-titanium (cp-Ti) plates, we evaluated the distribution of biomolecules by immunofluorescence. PPARγ, PDGF-B, and TGFβ1 were similarly released at immunofluorescence levels from activated adherent platelets, retained in the surrounding extra-platelet spaces for a while, and did not immediately diffuse away to distant spaces. Exogenously added CaCl2 augmented release and retention of those biomolecules along with activation and aggregation. Taken together with our previous data regarding platelet adhesion, these findings suggest that especially when treated with CaCl2, platelets immediately adhere on cp-Ti plates to release their stored biomolecules in the absence of plasma proteins and that these biomolecules do not diffuse away, but stay longer in extra-platelet spaces around the platelets by newly formed, immature fibrin fiber fragments. Consequently, these retained biomolecules are anticipated to cooperatively stabilize implants by stimulating alveolar bone regeneration and integration.

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The Construction and Characterization of Nano-FHA Bioceramic Coating on Titanium Surface

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.330-332.333 ◽

2007 ◽

Vol 330-332 ◽

pp. 333-336 ◽

Cited By ~ 2

Author(s):

Xiao Xiao Cai ◽

Ping Gong ◽

Yi Man ◽

Zhi Qing Chen ◽

Gang He

Keyword(s):

Titanium Surface ◽

Sol Gel ◽

Pure Titanium ◽

Cellular Responses ◽

Commercially Pure Titanium ◽

X Ray Diffraction ◽

Commercially Pure ◽

Scale Surface

This research was aimed at the construction and characterization of nano-FHA bioceramic coating on titanium surface. Nano-FHA coating was constructed on the surface of commercially pure titanium by sol-gel route. X-ray diffraction (XRD), scanning electromicroscope (SEM) and dissolution test was employed to characterize the obtained coating. In vitro cellular responses of osteoblasts to the coating were also evaluated by MTT assay, ALP assay and SEM observation. Conventional HA coatings and commercially pure titanium (cpTi) were taken as control. Results show the nano-FHA bioceramic coating has good crystallization and homogeneous, nano-scale surface morphology. The dissolution rate of the coating is favorable. The in vitro osteoblasts culture exhibits satisfactory bioactivity.

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The qualitative effects of various types of hygiene instrumentation on commercially pure titanium and titanium alloy implant abutments: an in vitro and scanning electron microscope study

Journal of Prosthetic Dentistry ◽

10.1016/s0022-3913(97)70028-3 ◽

1997 ◽

Vol 78 (3) ◽

pp. 286-294 ◽

Cited By ~ 21

Author(s):

Frieda Von Giese Brookshire ◽

William W. Nagy ◽

Virendra B. Dhuru ◽

Gerald J. Ziebert ◽

Srinivas Chada

Keyword(s):

Titanium Alloy ◽

Electron Microscope ◽

Electron Microscope Study ◽

Pure Titanium ◽

Commercially Pure Titanium ◽

Scanning Electron Microscope Study ◽

Commercially Pure ◽

Implant Abutments ◽

Scanning Electron

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Retention Strength of Cobalt-Chromium vs Nickel-Chromium Titanium vs CP Titanium in a Cast Framework Association of Removable Partial Overdenture

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1031 ◽

2011 ◽

Vol 12 (3) ◽

pp. 179-186

Author(s):

Adriana Cristina Zavanelli ◽

Recardo Alexandre Zavanelli ◽

José Everaldo de Aquino Souza ◽

Nelson Renato França Alves da Silva ◽

Paulo Guilherme Coelho ◽

...

Keyword(s):

Potential Risk ◽

Artificial Saliva ◽

Pure Titanium ◽

Commercially Pure Titanium ◽

Cobalt Chromium ◽

Nickel Chromium ◽

Commercially Pure ◽

Significant Difference ◽

Retention Strength

ABSTRACT Aim There is little information considering the framework association between cast clasps and attachments. The aim of this study was to evaluate the retention strength of frameworks match circumferential clasps and extra resilient attachment cast in three different alloys (cobalt-chromium, nickel-chromium titanium and commercially pure titanium), using two undercut (0.25 and 0.75 mm) and considering different period of time (0, 1/2, 1, 2, 3, 4 and 5 years). Methods Using two metallic matrices, representing a partially edentulous mandibular right hemiarch with the first molar crown, canine root and without premolars, 60 frameworks were fabricated. Three groups (n = 20) of each metal were cast and each group was divided into two subgroups (n = 10), corresponding the molar undercut of 0.25 mm and 0.75 mm. The nylon male was positioned at the matrix and attached to the acrylic resin of the prosthetic base. The samples were subjected to an insertion and removal test under artificial saliva environment. Results The data were analyzed and compared with ANOVAs and Tukey's test at 95% of probability. The groups cast in cobaltchromium and nickel-chromium-titanium had the highest mean retention strength (5.58 N and 6.36 N respectively) without significant difference between them, but statistically different from the group cast in commercially pure titanium, which had the lowest mean retention strength in all the periods (3.46 N). The association frameworks using nickel-chromium- titanium and cobalt-chromium could be used with 0.25 mm and 0.75 mm of undercut, but the titanium samples seems to decrease the retention strength, mainly in the 0.75 mm undercut. The circumferential clasps cast in commercially pure titanium used in 0.75 mm undercuts have a potential risk of fractures, especially after the 2nd year of use. Conclusion This in vitro study showed that the framework association between cast clasp and an extra resilient attachment are suitable to the three metals evaluated, but strongly suggest extra care with commercially pure titanium in undercut of 0.75 mm. Clinical significance Frameworks fabricated in Cp Ti tend to decrease in retentive strength over time and have a potential risk of fracture in less than 0.75 mm of undercut. How to cite this article Souza JEdA, da Silva NRFA, Coelho PG, Zavanelli AC, Ferracioli RCSR, Zavanelli RA. Retention Strength of Cobalt-Chromium vs Nickel-Chromium Titanium vs CP Titanium in a Cast Framework Association of Removable Partial Overdenture. J Contemp Dent Pract 2011;12(3):179-186.

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