scholarly journals Tuberculous meningoencephalitis with severe neurological sequel in an immigrant child

2011 ◽  
Vol 02 (01) ◽  
pp. 077-079 ◽  
Author(s):  
Kadriye Kart Yasar ◽  
Filiz Pehlivanoglu ◽  
Gonul Sengoz ◽  
E Rahsan Ince ◽  
Semra Sandikci
Keyword(s):  
Positive Family History ◽  
Diagnostic Tools ◽  
Family Migration ◽  
Chain Reaction ◽  
Mortality And Morbidity ◽  
Delayed Treatment ◽  
Tb Control ◽  
Polymerase Chain ◽  
Neurological Sequel ◽  
Prompt Diagnosis

ABSTRACTCentral nervous system tuberculosis (TB) is the most devastating manifestation of TB. It is a challenge for clinicians because of the diffi culty in making an early diagnosis and the severe consequences of delayed treatment. The aim of this report is to point out the relation between migration and TB based on a 14-year-old child with tuberculous meningoencephalitis (TBM) of an immigrant family. Migration, crowded living conditions and positive family history contribute to the severe course of TB as TBM and miliary TB forms. TB control may prevent these severe manifestations of the disease among immigrants. Prompt diagnosis with helpful early diagnostic tools like polymerase chain reaction in TBM is crucial due to the high mortality and morbidity.

scholarly journals The management of postoperative COVID-19 pneumonia in a case with right pneumonectomy

2021 ◽  
Vol 29 (2) ◽  
pp. 275-278
Author(s):  
Bülent Mustafa Yenigün ◽  
Farrukh Ibrahimov ◽  
Yusuf Kahya ◽  
Gökhan Kocaman ◽  
Ezgi Gülten ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Polymerase Chain Reaction ◽  
Case Report ◽  
Reverse Transcription ◽  
Lung Mass ◽  
Chain Reaction ◽  
Mortality And Morbidity ◽  
Thoracic Computed Tomography ◽  
Polymerase Chain ◽  
Prompt Diagnosis

A 61-year-old male patient was hospitalized for the evaluation of a lung mass. The patient underwent right pneumonectomy. Although reverse-transcription polymerase chain reaction tests were negative for COVID-19, the diagnosis was supported by thoracic computed tomography. The patient responded to COVID-19 treatment, as evidenced by thoracic computed tomography. This case report highlights the importance of prompt diagnosis and treatment of COVID-19 in a patient who underwent pneumonectomy, which has high mortality and morbidity rates.

A Study of the Efficiency of Modern Domestic Disinfectants in the System of TB Control Activities

2015 ◽  
Vol 2 (2) ◽  
pp. 26-31 ◽  
Author(s):  
A. Paliy ◽  
A. Zavgorodniy ◽  
B. Stegniy ◽  
A. Gerilovych
Keyword(s):  
Molecular Genetic ◽  
Critical Role ◽  
Bactericidal Effect ◽  
Chain Reaction ◽  
Tb Control ◽  
Source Of Infection ◽  
Polymerase Chain ◽  
And Control ◽  
Chemical Groups ◽  
Test Objects

Due to the absence of elaborated effi cient means for specifi c prevention of bovine tuberculosis, it is ex- tremely important to detect and eliminate the source of infection and to take veterinary and sanitary preven- tive measures. Here the critical role is attributed to disinfection, which breaks the epizootic chain due to the elimination of pathogenic microorganisms in the environment and involves the application of disinfectants of different chemical groups. Aim. To study the tuberculocidal properties of new disinfectants DZPT-2 and FAG against atypical mycobacteria Mycobacterium fortitum and a TB agent Mycobacterium bovis. Methods. The bacteriological and molecular-genetic methods were used. Results. It was determined that DZPT-2 prepara- tion has bactericidal effect on M. fortuitum when used in the concentration of 2.0 % of the active ingredient (AI) when exposed for 5–24 h, while disinfectant FAG has a bactericidal effect in the concentration of 2.0 % when exposed for 24 h. Disinfectant DZPT-2 in the concentration of 2.0 % of the AI, when exposed for 5–24 h, and FAG preparation in the concentration of 2.0 %, when exposed for 24 h, and with the norm of consump- tion rate of 1 cubic decimeter per 1 square meter disinfect the test-objects (batiste, wood, glazed tile, metal, glass), contaminated with the TB agent M. bovis. Conclusions. Disinfecting preparations of DZPT-2 in the concentration of 2.0 % of AI when exposed for 5 h and FAG in the concentration of 2.0 % when exposed for 24 h may be used in the complex of veterinary and sanitary measures to prevent and control TB of farm ani- mals. The possibility of using the polymerase chain reaction as an additional method of estimating tuberculo- cide activity of disinfectants was proven.

scholarly journals Comparative Study of Immune-Diagnostic Tools with Polymerase Chain Reaction in Sub-Clinical Leishmaniasis Isolates

2011 ◽  
Vol 12 (1) ◽  
pp. 34-39 ◽  
Author(s):  
Nazar M Abdalla
Keyword(s):  
Polymerase Chain Reaction ◽  
Skin Test ◽  
Diagnostic Methods ◽  
Diagnostic Tools ◽  
Enzyme Linked Immunosorbent Assay ◽  
Clinical Form ◽  
Chain Reaction ◽  
Leishmanin Skin Test ◽  
Wide Range ◽  
Polymerase Chain

Objective: This study aimed to identify cases of leishmaniasis in the Nuba Mountain area, which is situated in a unique geographical site located in the centre of Sudanese leishmania belt. Wide range of investigations are available for detection of leishmania cases, but still the most reliable and easy test used as screening and epidemiological tool in field studies needs to be evaluated. The most commonly used conventional diagnostic methods direct microscopy and culture have some drawbacks in diagnosing subclinical cases of leishmaniasis. Materials and methods: In this study, comparative properties of various immune-diagnostic tools with Polymerase Chain Reaction used in sub-clinical leishmaniasis isolates were explored. The immune-diagnostic tools involved in this study include- Leishmanin Skin Test (LST), Enzyme Linked Immunosorbent Assay (ELISA) and Direct Agglutination Test (DAT). The study was conducted in the Green Valley village (Rashad Province, South Kordofan State) with a population of 332. Most of the villagers presented with sub-clinical form of leishmaniasis with minor symptoms and signs without the features found in clinical form of visceral leishmaniasis such as fever, diarrhoea, epistaxis, enlarged lymph nodes, spleen and liver. In this study we collected demographic, clinical and epidemiological data using special questionnaire. Leishmanin skin test (LST), ELISA, DAT and PCR for parasite DNA detection were used. Result: The final positive cases detected by PCR were 32 out of 332 belong to L. donovani species. The final positive cases detected by LST were 51.2% of the total population under study, while 11 out of the 37 tested samples were positive by ELISA. All of the 332 villagers showed negative readings by DAT with exception of three individuals who were positive with very high titers. Conclusion: DNA etxtraction and amplification with primers can be a good screening tool in subclinical leishmaniasis isolates. Keyword: Sub-clinical; Leishmaniasis; Leishmanin Skin Test; ELISA; DAT; PCR. DOI: 10.3329/jom.v12i1.5422J Medicine 2011; 12 : 34-39

Application of laser capture microdissection and polymerase chain reaction in the diagnosis of Trichoderma longibrachiatum infection: a promising diagnostic tool for ‘fungal contaminants’ infection

2019 ◽  
Vol 58 (3) ◽  
pp. 315-321
Author(s):  
Ya Bin Zhou ◽  
Gong Jie Zhang ◽  
Ying Gai Song ◽  
Li Na Sun ◽  
Ya Hong Chen ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Laser Capture Microdissection ◽  
Diagnostic Tool ◽  
Diagnostic Tools ◽  
Identical Result ◽  
Chain Reaction ◽  
Trichoderma Species ◽  
Polymerase Chain ◽  
Fungal Contaminants ◽  
Laser Capture

Abstract Although Trichoderma species are usually considered to be culture contaminants, an increasing number of case reports have demonstrated their pathogenicity. Current diagnostic tools, including fungal culture, radiology, histopathology, and direct microscopy examination, are often unable to differentiate the pathogenicity of ‘fungal contaminants’ such as Trichoderma species in patients. Accurate diagnostic tools for ‘fungal contaminants’ infection have become the urgent needs. To that end, we applicated laser capture microdissection (LCM) and polymerase chain reaction (PCR) to confirm T. longibrachiatum infection for the first time. A 57-year-old man presented with a cough and hemoptysis lasting for more than 40 days. Computed tomography scan revealed a mass at the left hilum. In addition to pulmonary spindle cell carcinoma, fungal hyphae were also detected in histopathological examination. The cultured fungus was identified as T. longibrachiatum using molecular procedures. The results from DNA sequencing of DNA obtained by LCM revealed the identical result. Antifungal susceptibility testing revealed resistance to itraconazole, fluconazole and flucytosine. The patient was managed with oral voriconazole for 4 months. No relapse of Trichoderma infection was observed at a year follow-up visit. Although there are potential disadvantages, LCM-based molecular biology technology is a promising diagnostic tool for ‘fungal contaminants’ infection.

scholarly journals Diagnostic Tools for Borrelia Assessment in Humans

2016 ◽  
Vol 10 (1) ◽  
pp. 62-69 ◽  
Author(s):  
Serena Bonin
Keyword(s):  
Polymerase Chain Reaction ◽  
Lyme Disease ◽  
Web Sites ◽  
Clinical Manifestations ◽  
Review Article ◽  
Diagnostic Tools ◽  
Chain Reaction ◽  
Wide Range ◽  
Controversial Topic ◽  
Polymerase Chain

Although the etiological agent of Lyme disease has been known since 1980s, diagnosis of Lyme disease is still a controversial topic because of the wide range of clinical manifestations and the limited diagnostic tools available to assessBorreliain humans.The most used diagnostic tool for Lyme disease is currently serology, but also Polymerase chain reaction (PCR) and other methods are often used to proveBorreliainfection in different patients’ specimens. The present article deals with most of the diagnostic tools used in clinical practice for Lyme disease detection in human samples. Direct and indirect specific methods forBorreliainfection detection will be discussed.The most recent peer reviewed publications as well as original results from our study and information provided by companies’ web sites have been analyzed to compile this review article.

scholarly journals Microscopy and Polymerase Chain Reaction Detection ofLeishmania chagasiin the Pleural and Ascitic Fluid of a Patient with AIDS: Case Report and Review of Diagnosis and Therapy of Visceral Leishmaniasis

2004 ◽  
Vol 15 (4) ◽  
pp. 231-234 ◽  
Author(s):  
Ada RS Diehl ◽  
Rodrigo P dos Santos ◽  
Ricardo Zimmerman ◽  
Letícia P Luz ◽  
Tanara Weiss ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Visceral Leishmaniasis ◽  
Ascitic Fluid ◽  
Clinical Manifestations ◽  
Histological Evaluation ◽  
Diagnostic Tools ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Brazilian Patient ◽  
Polymerase Chain

Atypical visceral leishmaniasis is increasingly reported in immunocompromised patients, including patients with AIDS. A case of visceral leishmaniasis in an HIV-infected Brazilian patient with pulmonary and peritoneal involvement is reported. Histological evaluation of pleural fluid and ascites aspirate revealed macrophages with intracellularLeishmania. Polymerase chain reaction analysis was positive forLeishmaniain the pleural and ascitic fluid with use of primers specific forLeishmania chagasi. In addition to classical methods for diagnosing leishmaniasis, such as microscopy and culture, polymerase chain reaction detection and identification ofLeishmaniaspecies in pleural effusions and ascites are important diagnostic tools that should be considered by clinicians evaluating HIV-infected patients from endemic areas of visceral leishmaniasis. The authors review the clinical manifestations, diagnostic and therapeutic aspects of visceral leishmaniasis in immunocompetent and HIV-infected patients.

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