Oxidative stress mitigation, kinetics of carbohydrate-enzymes inhibition and cytotoxic effects of flavonoids-rich leaf extract of Gazania krebsiana (Less.): An in vitro evaluation

Oxidative stress has gained attention as one of the fundamental mechanisms responsible for the development of hypertension. The present study investigated in vitro and in vivo antioxidant effects of 70% ethanol-water (v/v) leaf and root extracts of T. officinale (TOL and TOR, respectively). Total phenolic and flavonoid content of plant extracts were assessed using Folin Ciocalteau and aluminium chloride colorimetric methods; while, 2,2-diphenyl-1-picrlhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) protocols were used to determine the free radical scavenging and total antioxidant capacities (TAC), respectively. The in vivo total antioxidant capacity and malondialdehyde acid (MDA) levels for lipid peroxidation tests were performed on organ homogenate samples from Nω-nitro-L-arginine methyl ester (L-NAME)-induced hypertensive rats treated with leaf extract, TOL (500 mg/kg/day) and TOR (500 mg/kg/day) for 21 days. Results showed that compared to TOR, TOL possessed significantly higher (p < 0.01) polyphenol (4.35 ± 0.15 compared to 1.14 ± 0.01) and flavonoid (23.17 ± 0.14 compared to 3 ± 0.05) content; free radical scavenging activity (EC50 0.37 compared to 1.34 mg/mL) and total antioxidant capacities (82.56% compared to 61.54% ABTS, and 156 ± 5.28 compared to 40 ± 0.31 FRAP) and both extracts showed no toxicity (LD50 > 5000 mg/kg). TOL and TOR significantly (p < 0.01) elevated TAC and reduced MDA levels in targets organs. In conclusion, T. officinale leaf extract possesses significant anti-oxidant effects which conferred significant in vivo antioxidant protection against free radical-mediated oxidative stress in L-NAME-induced hypertensive rats.

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Antioxidant Effect of the Castanea sativa Mill. Leaf Extract on Oxidative Stress Induced upon Human Spermatozoa

Oxidative Medicine and Cellular Longevity ◽

10.1155/2019/8926075 ◽

2019 ◽

Vol 2019 ◽

pp. 1-9 ◽

Cited By ~ 3

Author(s):

Marco Biagi ◽

Daria Noto ◽

Maddalena Corsini ◽

Giulia Baini ◽

Daniela Cerretani ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Sperm Motility ◽

Leaf Extract ◽

Colorimetric Assay ◽

Castanea Sativa ◽

High Concentration ◽

Tem Analysis ◽

Dpph Test

This study was aimed at evaluating in vitro the effects of a 75% v/v ethanolic extract of leaves of Castanea sativa Mill. (var. Bastarda Rossa, Mount Amiata, Tuscany, Italy) on ejaculated human sperm. Total polyphenols and total flavonoids contained in the extract were determined by a colorimetric assay and HPLC-DAD. The DPPH test and electrochemistry were utilized to study the antioxidant activity of the extract. Swim-up-selected sperm from 8 healthy men were treated with the C. sativa leaf extract at different dilutions (1 : 100, 1 : 200, and 1 : 500), and sperm motility was assessed following the WHO guidelines. Swim-up-selected spermatozoa were incubated with 100 μM H2O2 to induce lipid peroxidation (LPO) and with H2O2 and the leaf extract (1 : 100, 1 : 200, and 1 : 500) to test the antioxidant activity of the extract. The levels of LPO were determined by measuring malondialdehyde (MDA) concentrations. The treated samples were also analyzed by transmission electron microscopy (TEM) for ultrastructural evaluation. The chemical analysis showed that one-third ca. of the polyphenols in the C. sativa extract were made up of flavonoids, with hyperoside present in high concentration. A good antioxidant activity was demonstrated by both the DPPH test and electrochemical analysis. The C. sativa leaf extract did not decrease sperm motility at all tested dilutions. Treatment with H2O2 alone caused a significant increment in MDA levels (P=0.006993), while the treatment with H2O2 plus C. sativa extract diluted to 1 : 100 and 1 : 200 significantly reduced MDA levels (P=0.01476 and P=0.01571, respectively), with respect to H2O2 alone. TEM analysis confirmed the protective effect of the extract on damage induced by LPO, in particular that occurring at the plasma membrane level. The C. sativa leaf extract could be used in human and farm animal protocols for gamete handling, such as techniques of assisted reproduction and cryopreservation of semen, all conditions in which oxidative stress is exacerbated.

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Erratum: Synthesis and in vitro evaluation of melatonin entrapped PLA nanoparticles: An oxidative stress and T-cell response using golden hamster. (J Biomed Mater Res Part A 2015:103A:3034-3044)

Journal of Biomedical Materials Research Part A ◽

10.1002/jbm.a.36340 ◽

2018 ◽

Vol 106 (3) ◽

pp. 858-858

Author(s):

Sanjeev K. Pandey ◽

Chandana Haldar ◽

Dipanshu K. Vishwas ◽

Pralay Maiti

Keyword(s):

Oxidative Stress ◽

T Cell ◽

Golden Hamster ◽

Cell Response ◽

T Cell Response ◽

In Vitro Evaluation ◽

Biomed Mater

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Moringa oleifera L. Extracts as Bioactive Ingredients That Increase Safety of Body Wash Cosmetics

Dermatology Research and Practice ◽

10.1155/2020/8197902 ◽

2020 ◽

Vol 2020 ◽

pp. 1-14

Author(s):

Zofia Nizioł-Łukaszewska ◽

Dominika Furman-Toczek ◽

Tomasz Bujak ◽

Tomasz Wasilewski ◽

Zofia Hordyjewicz-Baran

Keyword(s):

Oxidative Stress ◽

Leaf Extract ◽

Moringa Oleifera ◽

Skin Irritation ◽

Tree Leaves ◽

Model Formulation ◽

Different Types ◽

Bioactive Ingredients ◽

Positive Effect

The work attempts to obtain a multifunctional plant extract derived from Moringa tree leaves. Obtained results indicate a strong antioxidant potential of the tested extracts. It was shown that Moringa oleifera leaf extract is a rich source of flavonoid and phenolic compounds. Furthermore, it shows a strong antioxidant activity by scavenging free radicals. In vitro toxicity studies showed that the tested extracts in concentrations up to 5% showed a positive effect on cell proliferation and metabolism and may contribute to the reduction of oxidative stress in cells. It was noted that the tested model formulation of cosmetic (1% SCS) with the addition of different types of extracts might contribute to the reduction of skin irritation and improve the safety of the product.

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