Detection and categorization of biofilm-forming Staphylococcus aureus, Viridans streptococcus, Klebsiella pneumoniae, and Escherichia coli isolated from complete denture patients and visualization using scanning electron microscopy

Telah dilakukan penelitian tentang kemampuan aktivitas anti bakteri untuk bakteri Staphylococcus aureus (gram positif) dan Escherichia coli (gram negatif) dengan menggunakan nanokomposit nata de coco/TiO2, nata de coco/Ag, dan nata de coco/TiO2/Ag dengan variasi konsentrasi Ag 0,5 M; 0,6 M; 0,7 M; 0,8 M dan 0,9 M menggunakan metode reduksi kimia. Nanopartikel tersebut dikarakterisasi menggunakan X-Ray Diffractometry (XRD), scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDS) dan anti bakteri. Untuk uji aktivitas antibakteri menggunakan metode kertas cakram dan dilakukan sebanyak dua kali ulangan untuk tiap-tiap sampel dan bakteri yang diuji. Hasil penelitian menunjukan, aktivitas anti bakteri nanokomposit yang paling baik dalam menghambat pertumbuhan bakteri adalah nanokomposit Nata de coco/TiO2/Ag mampu menghambat pertumbuhan bakteri Escherichiacoli dan Staphylococcusaureus, namun nanokomposit tersebut lebih efektif menghambat pertumbuhan bakteri Escherichiacoli.ABSRACT Research on the ability of anti-bacterial activity for Staphylococcus aureus (gram positive) and Escherichia coli (gram negative) bacteria using nata de coco / nanocomposites TiO2, nata de coco / Ag, and nata de coco / TiO2 / Ag with variations of Ag 0,5 M; 0.6 M; 0.7 M; 0.8 M and 0.9 M using the chemical reduction method. Nanoparticles were characterized using X-Ray Diffractometry (XRD), scanning electron microscopy-energy dispersive X-ray spctroscopy (SEM-EDS) and anti-bacterial actvity. Test the antibacterial activity using the paper disc method and repeated two times for each sample and bacteria tested. The results showed that the good anti-bacterial activity of nanocomposites in inhibiting bacterial growth was nanocomposite nata de coco /TiO2/Ag able to inhibit the growth of Escherichia coli and S. aureus, but the nanocomposite is more effective in inhibiting the growth of Escherichia coli bacteria.

Download Full-text

Synthesis of nano-zinc oxide with different morphologies and its application on fabrics for UV protection and microbe-resistant defense clothing

Textile Research Journal ◽

10.1177/0040517520920952 ◽

2020 ◽

Vol 90 (21-22) ◽

pp. 2492-2503

Author(s):

MA Mousa ◽

M Khairy

Keyword(s):

Electron Microscopy ◽

Escherichia Coli ◽

Scanning Electron Microscopy ◽

Staphylococcus Aureus ◽

Zinc Oxide ◽

X Ray Diffraction ◽

X Ray ◽

Nano Zinc Oxide ◽

Nano Zinc ◽

Scanning Electron

A liquid precipitation method was used to prepare zinc oxide nanoparticles in three diverse media: water, methanol, and ethylene glycol. The studied materials were examined by scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and ultraviolet-visible spectroscopy. X-ray diffraction patterns showed a hexagonal Wurtzite structure of zinc oxide with a nanocrystalline size. Acquired powders showed different morphologies (rod, star, and spherical structures), which were affected by the nature of the solvent in the reaction. The different zinc oxide powders have varied optical band gaps. Scanning electron microscopy examinations confirmed the arrangement of nano-zinc oxide on the surfaces of the materials. The zinc oxide-covering procedure was carried out on cotton, polyester, and 50/50 wt% polyester/cotton blended fabrics using a simple dip and curing system. The cotton fabric treated with nanorod zinc oxide exhibited the highest ultraviolet protection factor with a value of 247.2. The antimicrobial properties of untreated and treated fabrics with nano-zinc oxide were measured against Gram-negative bacteria (Escherichia coli), Gram-positive bacteria (Staphylococcus aureus), and diploid fungus (Candida albicans). The results showed the antimicrobial action relies on the morphological structure and the particle size of zinc oxide and that it increases with a reduced particle size. The cotton fabric treated with 26 nm nonspherical zinc oxide particles showed the highest antimicrobial efficiency with values of 91.4%, 86.8%, and 84.7% for Staphylococcus aureus, Escherichia coli, and Candida albicans, respectively. The mechanical properties of treated fabrics were studied. The results confirm that nano-zinc oxide is highly useful for improving the performance of defense textile products because of its biocompatibility, environmental friendliness, and nontoxicity.

Download Full-text

SINTESIS DAN KARAKTERISASI NANOPARTIKEL CuFe2O4 SERTA APLIKASINYA SEBAGAI ANTIBAKTERI

CHEMISTRY PROGRESS ◽

10.35799/cp.12.2.2019.27929 ◽

2020 ◽

Vol 12 (2) ◽

Author(s):

Almendo G. Lasera ◽

Henry Aritonang ◽

Harry Koleangan

Keyword(s):

Electron Microscopy ◽

Escherichia Coli ◽

Scanning Electron Microscopy ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Gram Negative Bacteria ◽

E Coli ◽

Activity Test ◽

Sem Imaging ◽

Scanning Electron

ABSTRAKNanopartikel CuFe2O4 disintesis menggunakan metode kopresipitasi. Material yang telah disintesis, kemudian dikarakterisasi dengan menggunakan Scanning Electron Microscopy (SEM) dan Xray-Diffraction (XRD) serta di uji aktifitasnya sebagai antibakteri dengan menggunakan bakteri Staphylococcus aureus (Gram positif) dan Escherichia coli (Gram negatif). Hasil citra SEM memperlihatkan morfologi nanopartikel CuFe2O4 berbentuk persegi yang tidak beraturan. Difraktogram XRD menunjukkan bahwa ukuran sampel CuFe2O4 yang diperoleh adalah sebesar 20,136 nm. Uji aktifitas antibakteri yang telah dilakukan menunjukkan bahwa, nanopartikel CuFe2O4 jauh lebih baik dalam menghambat pertumbuhan bakteri E. coli dibandingkan dengan bakteri S.aureus, masing-masing 24 mm dan 6,5 mm. ABSTRACTCuFe2O4 nanoparticles were synthesized using the coprecipitation method. The synthesized material was then characterized using Scanning Electron Microscopy (SEM) and Xray-Difraction (XRD) and tested as antibacterial activity using Staphylococcus aureus (Gram positive) and Escherichia coli (Gram negative) bacteria. SEM imaging showed morphology of CuFe2O4 nanoparticles shaped like a rod. XRD diffractogram showed that the size of CuFe2O4 nanoparticles that obtained at 20.13 nm. The antibacterial activity test that has been done shows, CuFe2O4 nanoparticles are much better in inhibiting the growth of E. coli bacteria compared with S. aureus bacteria, 24 mm and 6,5 respertivelly.

Download Full-text

The Effect of silver and titanium dioxide nanoparticles on Klebsiella pneumoniae isolates multi resistant to antibiotics and observed by Scanning electron microscopy

10.24086/bios17.26 ◽

2017 ◽

Author(s):

Payman Kareem ◽

Essra Alsammak

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Titanium Dioxide ◽

Klebsiella Pneumoniae ◽

Titanium Dioxide Nanoparticles ◽

Scanning Electron

Download Full-text

Biological Evaluation of Selected 1,2,3-triazole Derivatives as Antibacterial and Antibiofilm Agents

Current Topics in Medicinal Chemistry ◽

10.2174/1568026620666200710104737 ◽

2020 ◽

Vol 20 (24) ◽

pp. 2186-2191

Author(s):

Lialyz Soares Pereira André ◽

Renata Freire Alves Pereira ◽

Felipe Ramos Pinheiro ◽

Aislan Cristina Rheder Fagundes Pascoal ◽

Vitor Francisco Ferreira ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Staphylococcus Aureus ◽

Biofilm Formation ◽

Antimicrobial Agents ◽

Public Health Problem ◽

Biological Evaluation ◽

Major Public Health Problem ◽

Community Environments ◽

Scanning Electron

Background: Resistance to antimicrobial agents is a major public health problem, being Staphylococcus aureus prevalent in infections in hospital and community environments and, admittedly, related to biofilm formation in biotic and abiotic surfaces. Biofilms form a complex and structured community of microorganisms surrounded by an extracellular matrix adhering to each other and to a surface that gives them even more protection from and resistance against the action of antimicrobial agents, as well as against host defenses. Methods: Aiming to control and solve these problems, our study sought to evaluate the action of 1,2,3- triazoles against a Staphylococcus aureus isolate in planktonic and in the biofilm form, evaluating the activity of this triazole through Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) tests. We have also performed cytotoxic evaluation and Scanning Electron Microscopy (SEM) of the biofilms under the treatment of the compound. The 1,2,3-triazole DAN 49 showed bacteriostatic and bactericidal activity (MIC and MBC 128 μg/mL). In addition, its presence interfered with the biofilm formation stage (1/2 MIC, p <0.000001) and demonstrated an effect on young preformed biofilm (2 MICs, p <0.05). Results: Scanning Electron Microscopy images showed a reduction in the cell population and the appearance of deformations on the surface of some bacteria in the biofilm under treatment with the compound. Conclusion: Therefore, it was possible to conclude the promising anti-biofilm potential of 1,2,3-triazole, demonstrating the importance of the synthesis of new compounds with biological activity.

Download Full-text

N-halamine antibacterial nanofibrous mats based on polyacrylonitrile and N-halamine for protective face masks

Journal of Engineered Fibers and Fabrics ◽

10.1177/1558925019843222 ◽

2019 ◽

Vol 14 ◽

pp. 155892501984322 ◽

Cited By ~ 1

Author(s):

Chengbo Huang ◽

Ying Liu ◽

Zhiguang Li ◽

Rong Li ◽

Xuehong Ren ◽

...

Keyword(s):

Electron Microscopy ◽

Escherichia Coli ◽

Scanning Electron Microscopy ◽

Air Permeability ◽

Escherichia Coli O157 ◽

Electrospinning Technique ◽

Antibacterial Materials ◽

Fourier Transformed Infrared Spectroscopy ◽

Biocidal Properties ◽

Scanning Electron

The main objective of this study was to develop antibacterial materials based on polyacrylonitrile for potential application in protective face masks to combat airborne pathogens. To achieve biocidal properties, 1-chloro-2, 2, 5, 5-tetramethyl-4-imidazolidinone as a kind of N-halamine was introduced into the polyacrylonitrile nanofibers by an electrospinning technique to form nanofibers by an electrospinning technique to form polyacrylonitrile/1-chloro-2, 2, 5, 5-tetramethyl-4-imidazolidinone-5% nanofibers. Scanning electron microscopy and Fourier transformed infrared spectroscopy were employed to characterize the structure of nanofibers. The antimicrobial efficacies of electrospinning nanofibers with 1-chloro-2, 2, 5, 5-tetramethyl-4-imidazolidinone against both Staphylococcus aureus and Escherichia coli O157:H7 were evaluated at different contact times. The antimicrobial efficacies against bioaerosol of S. aureus were also performed. The polyacrylonitrile/1-chloro-2, 2, 5, 5-tetramethyl-4-imidazolidinone-5% nanofibers possess excellent antimicrobial efficacies against bacteria bioaersol, and it has good air permeability.

Download Full-text

Influence of Titanium Alloying Element Substrata on Bacterial Adhesion

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.535-537.992 ◽

2012 ◽

Vol 535-537 ◽

pp. 992-995

Author(s):

Kun Mediaswanti ◽

Vi Khanh Truong ◽

Jafar Hasan ◽

Elena P. Ivanova ◽

Francois Malherbe ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Staphylococcus Aureus ◽

Bacterial Adhesion ◽

Infection Risk ◽

Bacterial Attachment ◽

Bacterial Strains ◽

Alloying Element ◽

Bacteria Adhesion ◽

Scanning Electron

Titanium and titanium alloys have been widely employed in many load-bearing orthopaedic applications due to their excellent strength and corrosion resistance. However, postimplantation infections might occur even though considerable studies have been made. Choosing a bio-friendly alloying element is one way to reduce infection risk. The aim of this study is to evaluate the extent of bacterial attachment on titanium, tantalum, niobium and tin surfaces. Two pathogenic bacterial strains, namely Staphylococcus aureus CIP 65.8T and Pseudomonas aeruginosa ATCC 9027, were used in this study. Quantification of bacterial attachment was performed using scanning electron microscopy. Results indicated that the surface chemistry and topography of the investigated materials significantly influence the degree of P. aeruginosa and S. aureus adhesion; however, surface wettability did not show a significant impact upon bacterial retention. In this study, tin was shown to be the most attractive material for bacteria adhesion but tantalum limits the bacterial adhesion. Therefore, it is suggested to limit the amount of tin as an titanium alloying element due to its nature to attract P. aeruginosa and S. aureus adhesion.

Download Full-text

Ultrastructural Study of Elaeis guineensis (Oil Palm) Leaf and Antimicrobial Activity of its Methanol Extract against Staphylococcus Aureus

Tropical Journal of Pharmaceutical Research ◽

10.4314/tjpr.v12i3.22 ◽

2016 ◽

Vol 12 (3) ◽

pp. 419-423 ◽

Cited By ~ 2

Author(s):

Amala Rajoo ◽

Sreenivasan Sasidharan ◽

Subramanion L Jothy ◽

Surash Ramanathan ◽

Sharif M Mansor

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Staphylococcus Aureus ◽

Transmission Electron Microscopy ◽

Antimicrobial Activity ◽

Methanol Extract ◽

Elaeis Guineensis ◽

Transmission Electron ◽

Inhibition Concentration ◽

Scanning Electron

Purpose: To evaluate the antimicrobial activity of the methanol extract of Elaeis guineensis leaf against Staphylococcus aureus and to determine the effect of extract treatment on the microstructure of the microbeMethods: The antimicrobial activity of the methanol leaf extract of the plant against S. aureus was examined using disc diffusion and broth dilution methods. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were carried out to determine the major alterations in the microstructure of S. aureus after treatment with the extract.Results: The extract showed a good antimicrobial activity against S. aureus with a minimum inhibition concentration (MIC) of 6.25 mg/mL and for Chloramphenicol was 30.00 ìg/mL. The main changes observed under SEM and TEM were structural disorganization of the cell membrane which occurred after 12 h and total collapse of the cell 36 h after exposure to the extract.Conclusion: We concluded that the methanolic extract of E. guineensis leaf exhibited good antimicrobial activity against S. aureus and this is supported by SEM and TEM.Keywords: Antimicrobial activity, Elaeis guineensis, Staphylococcus aureus, Scanning electron microscopy, Transmission electron microscopy

Download Full-text