Vaccination against follistatin affects reproductive potential in cycling gilts

2000 ◽  
Vol 80 (2) ◽  
pp. 337-342
Author(s):  
C. R. Christensen ◽  
M. J. Redmond ◽  
B. Laarveld
Keyword(s):  
Key Words ◽  
Litter Size ◽  
Antibody Titer ◽  
High Titer ◽  
Reproductive Potential ◽  
High Antibody ◽  
High Antibody Titer ◽  
Antibody Titers

Primiparous sows (gilts) were actively vaccinated against follistatin in an attempt to modify litter size. Forty-seven gilts were vaccinated four times against a recombinant porcine follistatin (FS) or a sham vaccine (CTL) and were allowed to mature naturally prior to breeding. At breeding, FS antibody titers ranged from 0 to 1:6400 in the FS vaccinated gilts, and were not detectable in the CTL gilts. Overall, follistatin vaccination did not affect the total number of pigs born live (FS = 10.9 ± 0.5, CTL = 10.3 ± 0.4), stillborn (FS = 0.4 ± 0.1, CTL = 0.4 ± 0.2) or mummified (FS = 0.1 ± 0.1, CTL = 0.3 ± 0.1). However, separation of the FS vaccinated gilts into low (≤1:400, n = 16) and high (>1:400, n = 7) titer groups revealed significant differences in piglets born alive (FS high titer = 12.9 ± 0.9, FS low titer = 10.0 ± 0.5: P = 0.01) and total number of piglets born (FS high titer = 13.0 ± 0.8, FS low titer = 10.8 ± 0.6: P = 0.08). This study shows that vaccination of gilts against follistatin increased litter size in those gilts which achieved a high antibody titer to follistatin. Key words: Swine, follistatin, immunoneutralization, fecundity, litter size

scholarly journals Neutralizing Anti-SARS-CoV-2 Antibody Titer and Reported Adverse Effects, in a Sample of Italian Nursing Home Personnel after Two Doses of the BNT162b2 Vaccine Administered Four Weeks Apart

Vaccines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 652
Author(s):  
Alberto Modenese ◽  
Stefania Paduano ◽  
Annalisa Bargellini ◽  
Rossana Bellucci ◽  
Simona Marchetti ◽  
...  
Keyword(s):  
Nursing Home ◽  
Adverse Effects ◽  
Antibody Titer ◽  
Healthcare Workers ◽  
Vaccination Campaign ◽  
Northern Italy ◽  
High Antibody ◽  
Protective Level ◽  
High Antibody Titer ◽  
Main Factors

Background: The immunization of healthcare workers (HCWs) plays a recognized key role in prevention in the COVID-19 pandemic: in Italy, the vaccination campaign began at the end of December 2020. A better knowledge of the on-field immune response in HCWs, of adverse effects and of the main factors involved is fundamental. Methods: We performed a study on workers at a nursing home in Northern Italy, vaccinated in January–February 2021 with two doses of the BNT162b2 vaccine four weeks apart, instead of the three weeks provided for in the original manufacturer protocol. One month after the second dose, the serological titer of IgG-neutralizing anti-RBD antibodies of the subunit S1 of the spike protein of SARS-CoV-2 was determined. The socio-demographic and clinical characteristics of the subjects and adverse effects of vaccination were collected by questionnaire. Results: In all of the workers, high antibody titer, ranging between 20 and 760 times the minimum protective level were observed. Titers were significantly higher in subjects with a previous COVID-19 diagnosis. Adverse effects after the vaccine were more frequent after the second dose, but no severe adverse effects were observed. Conclusions: The two doses of the BNT162b2 vaccine, even if administered four weeks apart, induced high titers of anti-SARS-CoV-2 neutralizing IgG in all the operators included in the study.

scholarly journals High Antibody Titer against Apical Membrane Antigen-1 Is Required to Protect against Malaria in the Aotus Model

PLoS ONE ◽  
2009 ◽  
Vol 4 (12) ◽  
pp. e8138 ◽  
Author(s):  
Sheetij Dutta ◽  
JoAnn S. Sullivan ◽  
Katharine K. Grady ◽  
J. David Haynes ◽  
Jack Komisar ◽  
...  
Keyword(s):  
Antibody Titer ◽  
Apical Membrane ◽  
High Antibody ◽  
Apical Membrane Antigen 1 ◽  
Apical Membrane Antigen ◽  
High Antibody Titer

Ascending myelitis with high antibody titer to herpes simplex virus in the cerebrospinal fluid

1982 ◽  
Vol 227 (3) ◽  
pp. 187-191 ◽  
Author(s):  
M. -L. Koskiniemi ◽  
A. Vaheri ◽  
V. Manninen ◽  
P. Nikki
Keyword(s):  
Cerebrospinal Fluid ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Antibody Titer ◽  
High Antibody ◽  
High Antibody Titer ◽  
Simplex Virus

scholarly journals KEMAMPUAN NETRALISASI KEKEBALAN TUBUH INDUK MELALUI KUNING TELUR AYAM PETELUR PADA VIRUS AI (H5N1) ISOLAT LAPANGAN

2020 ◽  
Vol 6 (2) ◽  
pp. 89
Author(s):  
Agung Puji Haryanto
Keyword(s):  
Antibody Titer ◽  
Neutralization Test ◽  
Egg Yolk ◽  
Maternal Antibody ◽  
Control Group ◽  
High Antibody ◽  
Field Isolates ◽  
High Antibody Titer ◽  
Serum Neutralization Test ◽  
Group 2

Day old chick in the first week of their life got the immunity from maternal antibody / immunoglobulin trough egg yolk. Hens with high antibody titer will decended the immunoglobulin to their harvest trough egg yolk. The research studied the capability of antibody anti H5N1 from egg yolk to neutralized H5N1 AIV.  Twenty hens were devided to five group, one group as  a control group were unvaccinated group and four other groups were vaccinated with H5N1 AI inactive vaccine produced by several  Indonesia vaccine company. Four H5N1 AI inactive vaccines code VS1, VC1, VV1 and VM1 were vaccinated twice to group 2, 3, 4 and 5. A week after second vaccination the egg were collected and analyzed the antibody titer against H5N1 AIV  by hemagglutination test using H5N1 AIV field isolates as standard virus (NG and LW)). The egg yolk were contain high antibody titer (above 26) collected and tested against H5N1 AIV field isolates NG and LW by serum neutralization test.  The result showed that H5N1 AIV NG isolate were able to neutralized by antibody anti H5N1 AIV from egg yolk produced by hens vaccinated with VS1, VC1, VV1 and VM1, but LW isolate were able to neutralized by showed that H5N1 AIV NG isolate were able to neutralized by antibody anti H5N1 AIV from egg yolk produced by hens vaccinated with VS1, VC1, and VM1.  It’s concluded that hens were vaccinated with H5N1 AI inactive vaccine were able to protect their off spring against H5N1 AIV from the field by transferred maternal antibody trough the egg yolk with titer above 26.

scholarly journals Development of the scheme of obtaining antibodies to the ribonucleoprotein of attenuated rabies virus

2019 ◽  
pp. 3-8 ◽  
Author(s):  
Yu. K. Gavrilova ◽  
S. V. Generalov ◽  
M. N. Kireev ◽  
N. A. Sharapova ◽  
E. G. Abramova ◽  
...  
Keyword(s):  
Cell Culture ◽  
Rabies Virus ◽  
Antibody Titer ◽  
Colloidal Gold ◽  
Target Component ◽  
High Antibody ◽  
Experimental Animals ◽  
Colloidal Gold Nanoparticles ◽  
High Antibody Titer ◽  
Dot Immunoassay

Aim. Isolation of ribonucleoprotein (RNP) of an attenuated rabies virus, develop schemes for immunizing animals with RNP-based preparations and determine the most effective scheme that allows obtaining serum with a high antibody titer to the RNP.Materials and methods. We used the transplantable cell line Vero, the strain rabies virus «Moscow 3253 Vero», adapted for reproduction on Vero, rabbits of the chinchilla breed. In order to obtain serums containing antibodies to the RNP of the rabies virus, experimental schemes have been proposed for immunizing animals with RNP, including with adjuvants: polyoxidonium and colloidal gold. The dynamics of the accumulation of antibodies to the RNP of the rabies virus in the blood serum of experimental animals was studied by dot-immunoassay.Results. The target component (RNP of the rabies virus) was isolated directly from the cytoplasm of the Vero cell culture infected with the rabies virus according to the modified M. Dastkhosh (2014) method, lyophilized and used in the development of preparations for immunizing experimental animals. In the study of the dynamics of the formation of antibodies to RNP of the rabies virus by the method of dot-immunoassay, the effectiveness of an adjuvant is established — colloidal gold nanoparticles ranging in size from 15 to 17 nm, the use of which makes it possible to increase the antibody titer by 2 times.Conclusion. The results obtained are of interest for further research related to the design of diagnostic products and the development of methodological techniques using such preparations.

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