Molecular characterization and expression patterns of PsSVP genes reveal distinct roles in flower bud abortion and flowering in tree peony (Paeonia suffruticosa)

Wang, S., Xue, J., Ahmadi, N., Holloway, P., Zhu, F., Ren, X. and Zhang, X. 2014. Molecular characterization and expression patterns of PsSVP genes reveal distinct roles in flower bud abortion and flowering in tree peony (Paeonia suffruticosa). Can. J. Plant Sci. 94: 1181–1193. Container culture and flower forcing are used for off-season production of tree peony for the Chinese Spring Festival. Storage of potted tree peony for 10 d at 12°C in a refrigerator before 4°C chilling treatment can help new root growth and promote leaf development. Development from bud swelling to anthesis was divided into nine stages. Some aborted flower buds usually emerge in Stage III. Removal of two to four leaflets in an alternating pattern and applying gibberellic acid 3 (GA3) around the flower bud at Stage III can decrease the flower bud abortion rate and promote flower formation rate. Two MADS-box genes with homology to Arabidopsis SVP, designated PsSVP1 and PsSVP2, which probably caused flower-bud abortion, were isolated by reverse transcription-PCR. Sequence comparison analysis showed that PsSVP was most similar to SVP-like gene in apple. Phylogenetic analysis indicates that PsSVP was evolutionarily close to SVP-like genes from Malus domestica, SVP genes from Cruciferae and SVP-like genes from Vitis vinifera. The qRT-PCR results suggested that expression of PsSVP was high in vegetative growth phase, especially in the leaves of tree peony, and its expression was regulated by GA3. Further analysis showed that more PsSVP transcripted in the aborted flower bud, especially in the buds where leaflets grew well. It was deduced that PsSVP can promote vegetative growth and suppress flowering in tree peony. Thus, it is very important to further investigate PsSVP and decipher the mechanisms of flower-bud abortion to improve forcing culture of tree peony.

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Molecular Characterization and Expression of Ethylene Biosynthetic Genes During Cut Flower Development in Tree Peony (Paeonia suffruticosa) in Response to Ethylene and Functional Analysis of PsACS1 in Arabidopsis thaliana

Journal of Plant Growth Regulation ◽

10.1007/s00344-012-9306-4 ◽

2012 ◽

Vol 32 (2) ◽

pp. 362-375 ◽

Cited By ~ 8

Author(s):

Lin Zhou ◽

Chao Zhang ◽

Jianxin Fu ◽

Miao Liu ◽

Yuhuan Zhang ◽

...

Keyword(s):

Functional Analysis ◽

Arabidopsis Thaliana ◽

Molecular Characterization ◽

Flower Development ◽

Cut Flower ◽

Paeonia Suffruticosa ◽

Tree Peony ◽

Biosynthetic Genes

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Transcriptome profiling for floral development in reblooming cultivar ‘High Noon’ of Paeonia suffruticosa

Scientific Data ◽

10.1038/s41597-019-0240-1 ◽

2019 ◽

Vol 6 (1) ◽

Author(s):

Yanting Chang ◽

Tao Hu ◽

Wenbo Zhang ◽

Lin Zhou ◽

Yan Wang ◽

...

Keyword(s):

Flower Development ◽

Floral Development ◽

Developmental Stages ◽

Transcriptome Profiling ◽

Full Length ◽

Rna Seq ◽

Paeonia Suffruticosa ◽

Tree Peony ◽

Flower Bud ◽

High Noon

Abstract Tree peony (Paeonia suffruticosa Andrew) is a popular ornamental plant due to its large, fragrant and colorful flowers. The floral development is the most important event in its lifecycle. To explore the mechanism that regulate flower development, we sequenced the flower bud transcriptomes of ‘High Noon’, a reblooming cultivar of P. suffruticosa × P. lutea, using both full-length isoform-sequencing (ISO-seq) and RNA-seq were sequenced. A total of 15.94 Gb raw data were generated in full-length transcriptome sequencing of the 3 floral developmental stages, resulting 0.11 M protein-coding transcripts. Over 457.0 million reads were obtained by RNA-seq in the 3 floral buds. Here, we openly released the full-length transcriptome database of ‘High Noon’ and RNA-seq database of floral development. These databases can provide a fundamental genetic information of tree peony to investigate its transcript structure, variants and evolution. Data will facilitate to deep analyses of the transcriptome for flower development.

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Analysis of SSR motifs in tree peony (Paeonia suffruticosa Andrews.) EST sequences

JOURNAL OF HUNAN AGRICULTURAL UNIVERSITY ◽

10.3724/sp.j.1238.2011.00172 ◽

2011 ◽

Vol 37 (2) ◽

pp. 172-176 ◽

Cited By ~ 1

Author(s):

Xiao-gai HOU ◽

Juan WANG ◽

Da-long GUO ◽

Gai-xiu LIU ◽

Hui-li MA ◽

...

Keyword(s):

Paeonia Suffruticosa ◽

Tree Peony

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Regulation of Flowering Timing by ABA-NnSnRK1 Signaling Pathway in Lotus

International Journal of Molecular Sciences ◽

10.3390/ijms22083932 ◽

2021 ◽

Vol 22 (8) ◽

pp. 3932

Author(s):

Jing Cao ◽

Qijiang Jin ◽

Jiaying Kuang ◽

Yanjie Wang ◽

Yingchun Xu

Keyword(s):

Protein Kinase ◽

Environmental Changes ◽

Molecular Genetic ◽

Maximal Response ◽

Protein Kinase Activity ◽

Hormonal Changes ◽

Flower Buds ◽

Flower Bud ◽

Aba Content ◽

Flower Bud Abortion

The lotus produces flower buds at each node, yet most of them are aborted because of unfavorable environmental changes and the mechanism remains unclear. In this work, we proposed a potential novel pathway for ABA-mediated flower timing control in the lotus, which was explored by combining molecular, genetic, transcriptomic, biochemical, and pharmacologic approaches. We found that the aborting flower buds experienced extensive programmed cell death (PCD). The hormonal changes between the normal and aborting flower buds were dominated by abscisic acid (ABA). Seedlings treated with increasing concentrations of ABA exhibited a differential alleviating effect on flower bud abortion, with a maximal response at 80 μM. Transcriptome analysis further confirmed the changes of ABA content and the occurrence of PCD, and indicated the importance of PCD-related SNF1-related protein kinase 1 (NnSnRK1). The NnSnRK1-silenced lotus seedlings showed stronger flowering ability, with their flower:leaf ratio increased by 40%. When seedlings were treated with ABA, the expression level and protein kinase activity of NnSnRK1 significantly decreased. The phenotype of NnSnRK1-silenced seedlings could also be enhanced by ABA treatment and reversed by tungstate treatment. These results suggested that the decline of ABA content in lotus flower buds released its repression of NnSnRK1, which then initiated flower bud abortion.

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Selection and validation of appropriate reference genes for RT-qPCR analysis of flowering stages and different genotypes of Iris germanica L

Scientific Reports ◽

10.1038/s41598-021-89100-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Yinjie Wang ◽

Yongxia Zhang ◽

Qingquan Liu ◽

Haiying Tong ◽

Ting Zhang ◽

...

Keyword(s):

Reference Genes ◽

Molecular Mechanisms ◽

Gene Selection ◽

Expression Patterns ◽

Herbaceous Plant ◽

Flower Bud ◽

Perennial Herbaceous Plant ◽

Iris Germanica ◽

Accurate Normalization ◽

Suitable Reference

AbstractIris germanica L. is a perennial herbaceous plant that has been widely cultivated worldwide and is popular for its elegant and vibrantly colorful flowers. Selection of appropriate reference genes is the prerequisite for accurate normalization of target gene expression by quantitative real-time PCR. However, to date, the most suitable reference genes for flowering stages have not been elucidated in I. germanica. In this study, eight candidate reference genes were examined for the normalization of RT-qPCR in three I. germanica cultivars, and their stability were evaluated by four different algorithms (GeNorm, NormFinder, BestKeeper, and Ref-finder). The results revealed that IgUBC and IgGAPDH were the most stable reference genes in ‘00246’ and ‘Elizabeth’, and IgTUB and IgUBC showed stable expression in ‘2010200’. IgUBC and IgGAPDH were the most stable in all samples, while IgUBQ showed the least stability. Finally, to validate the reliability of the selected reference genes, the expression patterns of IgFT (Flowering Locus T gene) was analyzed and emphasized the importance of appropriate reference gene selection. This work presented the first systematic study of reference genes selection during flower bud development and provided guidance to research of the molecular mechanisms of flowering stages in I. germanica.

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Molecular characterization and expression patterns of adenosine deaminases acting on tRNAs in Dabry’s sturgeon ( Acipenser dabryanus )

Journal of Applied Ichthyology ◽

10.1111/jai.14293 ◽

2021 ◽

Author(s):

Qian Li ◽

Huizhi Guo ◽

Shuhuan Zhang ◽

Hanwen Yuan ◽

Jiang Zhou ◽

...

Keyword(s):

Molecular Characterization ◽

Expression Patterns ◽

Adenosine Deaminases ◽

Dabry’S Sturgeon

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Genome-Wide Analysis of the NAC Transcription Factor Gene Family Reveals Differential Expression Patterns and Cold-Stress Responses in the Woody Plant Prunus mume

Genes ◽

10.3390/genes9100494 ◽

2018 ◽

Vol 9 (10) ◽

pp. 494 ◽

Cited By ~ 17

Author(s):

Xiaokang Zhuo ◽

Tangchun Zheng ◽

Zhiyong Zhang ◽

Yichi Zhang ◽

Liangbao Jiang ◽

...

Keyword(s):

Cold Stress ◽

Gene Family ◽

Cold Tolerance ◽

Stress Responses ◽

Expression Profiles ◽

Expression Patterns ◽

Freezing Stress ◽

Prunus Mume ◽

Flower Bud ◽

Freezing Resistance

NAC transcription factors (TFs) participate in multiple biological processes, including biotic and abiotic stress responses, signal transduction and development. Cold stress can adversely impact plant growth and development, thereby limiting agricultural productivity. Prunus mume, an excellent horticultural crop, is widely cultivated in Asian countries. Its flower can tolerate freezing-stress in the early spring. To investigate the putative NAC genes responsible for cold-stress, we identified and analyzed 113 high-confidence PmNAC genes and characterized them by bioinformatics tools and expression profiles. These PmNACs were clustered into 14 sub-families and distributed on eight chromosomes and scaffolds, with the highest number located on chromosome 3. Duplicated events resulted in a large gene family; 15 and 8 pairs of PmNACs were the result of tandem and segmental duplicates, respectively. Moreover, three membrane-bound proteins (PmNAC59/66/73) and three miRNA-targeted genes (PmNAC40/41/83) were identified. Most PmNAC genes presented tissue-specific and time-specific expression patterns. Sixteen PmNACs (PmNAC11/19/20/23/41/48/58/74/75/76/78/79/85/86/103/111) exhibited down-regulation during flower bud opening and are, therefore, putative candidates for dormancy and cold-tolerance. Seventeen genes (PmNAC11/12/17/21/29/42/30/48/59/66/73/75/85/86/93/99/111) were highly expressed in stem during winter and are putative candidates for freezing resistance. The cold-stress response pattern of 15 putative PmNACs was observed under 4 °C at different treatment times. The expression of 10 genes (PmNAC11/20/23/40/42/48/57/60/66/86) was upregulated, while 5 genes (PmNAC59/61/82/85/107) were significantly inhibited. The putative candidates, thus identified, have the potential for breeding the cold-tolerant horticultural plants. This study increases our understanding of functions of the NAC gene family in cold tolerance, thereby potentially intensifying the molecular breeding programs of woody plants.

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