PRELIMINARY OBSERVATIONS ON BIOLOGICAL INSECTICIDES AGAINST IMPORTED CABBAGEWORM

1966 ◽  
Vol 46 (5) ◽  
pp. 497-499 ◽  
Author(s):  
C. J. S. Fox ◽  
R. P. Jaques
Keyword(s):  
Nova Scotia ◽  
Bacillus Thuringiensis ◽  
Parasitic Nematode ◽  
Pieris Rapae ◽  
Granulosis Virus ◽  
Adequate Control ◽  
Bacterium Bacillus

At Kentville, Nova Scotia, in 1963 and 1965, three biological insecticides were evaluated in the field for control of the imported cabbageworm, Pieris rapae (L.) (Lepidoptera:Pieridae). One or two spray treatments were applied at 9- or 14-day intervals. All treatments significantly reduced numbers of the insect. The bacterium Bacillus thuringiensis Berliner applied once gave the best control at first but in 1965 did not maintain adequate control to harvest. A granulosis virus was as effective as DDT followed by an application of mevinphos. The parasitic nematode DD136 gave control late in the season.

EFFICACY OF MIXTURES OF BACILLUS THURINGIENSIS, VIRUSES, AND CHLORDIMEFORM AGAINST INSECTS ON CABBAGE

1978 ◽  
Vol 110 (4) ◽  
pp. 443-448 ◽  
Author(s):  
R. P. Jaques ◽  
D. R. Laing
Keyword(s):  
Bacillus Thuringiensis ◽  
Trichoplusia Ni ◽  
Pieris Rapae ◽  
Autographa Californica ◽  
Granulosis Virus ◽  
Growing Seasons ◽  
Low Concentrations ◽  
Materials Used ◽  
Nuclear Polyhedrosis ◽  
Field Plots

AbstractThe efficacy of mixtures of chlordimeform with Bacillus thuringiensis and(or) Autographa californica nuclear polyhedrosis virus and Pieris rapae granulosis virus against Trichoplusia ni and Pieris rapae on the late cabbage was assessed in field plots over four growing seasons. Mixtures of 0.07 kg of chlordimeform/ha (1/8 of the recommended dosage) with low concentrations of the microbial insecticides were as effective or more effective than the materials used alone at full rates. The advantages of such mixtures in pest management are discussed.

FIELD TESTS WITH BACILLUS THURINGIENSIS BERLINER AND DDT FOR CONTROL OF TWO PESTS OF CABBAGE

1961 ◽  
Vol 41 (2) ◽  
pp. 428-430 ◽  
Author(s):  
C. J. S. Fox ◽  
R. P. Jaques
Keyword(s):  
Nova Scotia ◽  
Bacillus Thuringiensis ◽  
Pieris Rapae ◽  
Diamondback Moth ◽  
Field Tests ◽  
Good Control ◽  
Commercial Preparation

At Kentville, Nova Scotia, during 1960, seven spray schedules including a commercial preparation of Bacillus thuringiensis Berliner and DDT were evaluated in the field for control of the imported cabbageworm, Pieris rapae (L.), and larvae of the diamondback moth, Plutella maculipennis (Curt.). Up to three spray treatments were applied at 2-week intervals. Three applications of B. thuringiensis or one application of DDT followed by two of B. thuringiensis gave good control of both insects. In general, DDT and B. thuringiensis were equally effective against imported cabbageworm but DDT was more effective against larvae of the diamondback moth.

scholarly journals THE USE OF AGENTS BASED ON THE BACTERIUM BACILLUS THURINGIENSIS VAR. ISRAELENSIS FOR MOSQUITO CONTROL

2019 ◽  
Vol 98 (8) ◽  
pp. 893-896
Author(s):  
Svetlana A. Roslavtseva
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Synergistic Effect ◽  
Mosquito Control ◽  
Mosquito Species ◽  
Bacillus Sphaericus ◽  
Development Of Resistance ◽  
Blood Sucking ◽  
Low Concentrations ◽  
Bacterium Bacillus

Mosquito control is necessary to improve the epidemic and, consequently, the sanitary and hygienic situation in human settlements. At the same time, the safest and more environmentally friendly way of controlling is not the fight against adult mosquitoes, but the treatment of reservoirs with microbiological larvicides based on entomopathogenic, aerobic, spore-forming, saprophytic bacteria Bacillus thuringiensis (de Barjac) (Bti). A new serotype of the bacterium B. thuringiensis was found in Israel in the Negev desert. This serotype being more active against larvae of blood-sucking and non-blood-sucking mosquitoes and midges than previously known serotypes, was named israelensis. Bti endotoxin is a typical insecticide with intestinal type of action for different mosquito species. For example, Bti H14 is highly insecticidal to the larvae of Aedes aegypti and Ae. albopictus at very low concentrations. The parasporal body (endotoxin crystal), a crystalline protein consisted of four main polypeptides and two minor polypeptides, possesses of a larvicidal action. Larvicidal activity is associated with a synergistic effect in a combination of four polypeptides. The possibility of development of resistance to products based on Bti and Bacillus sphaericus in populations of mosquitoes (Culicidae) was investigated. The use of domestic microbiological formulations based on Bti («Baktitsid», «Larviol-pasta», and «Antinat») was shown an eradication the larvae of bloodsucking mosquitoes and midges to be possible and rational, since they are not generated resistant populations of mosquitoes. This is confirmed by more than 30 years of the use of such formulations.

scholarly journals Enhanced Degradation of Naproxen by Immobilization of Bacillus thuringiensis B1(2015b) on Loofah Sponge

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 872 ◽  
Author(s):  
Anna Dzionek ◽  
Danuta Wojcieszyńska ◽  
Małgorzata Adamczyk-Habrajska ◽  
Urszula Guzik
Keyword(s):  
Bacillus Thuringiensis ◽  
System Analysis ◽  
Treatment Plant ◽  
Trickling Filter ◽  
Significant Drop ◽  
Trickling Filters ◽  
Degrading Bacterium ◽  
Loofah Sponge ◽  
Immobilised Cells ◽  
Bacterium Bacillus

The naproxen-degrading bacterium Bacillus thuringiensis B1(2015b) was immobilised onto loofah sponge and introduced into lab-scale trickling filters. The trickling filters constructed for this study additionally contained stabilised microflora from a functioning wastewater treatment plant to assess the behavior of introduced immobilized biocatalyst in a fully functioning bioremediation system. The immobilised cells degraded naproxen (1 mg/L) faster in the presence of autochthonous microflora than in a monoculture trickling filter. There was also abundant colonization of the loofah sponges by the microorganisms from the system. Analysis of the influence of an acute, short-term naproxen exposure on the indigenous community revealed a significant drop in its diversity and qualitative composition. Bioaugmentation was also not neutral to the microflora. Introducing a new microorganism and increasing the removal of the pollutant caused changes in the microbial community structure and species composition. The incorporation of the immobilised B1(2015b) was successful and the introduced strain colonized the basic carrier in the trickling filter after the complete biodegradation of the naproxen. As a result, the bioremediation system could potentially be used to biodegrade naproxen in the future.

scholarly journals Bacillus thuringiensis Bioinsecticides Induce Developmental Defects in Non-Target Drosophila melanogaster Larvae

Insects ◽  
2020 ◽  
Vol 11 (10) ◽  
pp. 697
Author(s):  
Marie-Paule Nawrot-Esposito ◽  
Aurélie Babin ◽  
Matthieu Pasco ◽  
Marylène Poirié ◽  
Jean-Luc Gatti ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Bacillus Thuringiensis ◽  
Developmental Time ◽  
Probiotic Bacterium ◽  
Intestinal Cell ◽  
Cellular Mechanisms ◽  
Developmental Defects ◽  
Insecticidal Toxins ◽  
Bacterium Bacillus ◽  
Protein Rich

Bioinsecticides made from the bacterium Bacillus thuringiensis (Bt) are the bestselling bioinsecticide worldwide. Among Bt bioinsecticides, those based on the strain Bt subsp. kurstaki (Btk) are widely used in farming to specifically control pest lepidopteran larvae. Although there is much evidence of the lack of acute lethality of Btk products for non-target animals, only scarce data are available on their potential non-lethal developmental adverse effects. Using a concentration that could be reached in the field upon sprayings, we show that Btk products impair growth and developmental time of the non-target dipteran Drosophila melanogaster. We demonstrate that these effects are mediated by the synergy between Btk bacteria and Btk insecticidal toxins. We further show that Btk bioinsecticides trigger intestinal cell death and alter protein digestion without modifying the food intake and feeding behavior of the larvae. Interestingly, these harmful effects can be mitigated by a protein-rich diet or by adding the probiotic bacterium Lactobacillus plantarum into the food. Finally, we unravel two new cellular mechanisms allowing the larval midgut to maintain its integrity upon Btk aggression: First the flattening of surviving enterocytes and second, the generation of new immature cells arising from the adult midgut precursor cells. Together, these mechanisms participate to quickly fill in the holes left by the dying enterocytes.

scholarly journals Bacillus thuringiensis and Its Pesticidal Crystal Proteins

1998 ◽  
Vol 62 (3) ◽  
pp. 775-806 ◽  
Author(s):  
E. Schnepf ◽  
N. Crickmore ◽  
J. Van Rie ◽  
D. Lereclus ◽  
J. Baum ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Integrated Management ◽  
Management Strategies ◽  
Resistance Mechanisms ◽  
Maximum Efficiency ◽  
Crystal Proteins ◽  
Evolution Of Resistance ◽  
Complex Relationships ◽  
Bacterium Bacillus ◽  
Natural Settings

SUMMARY During the past decade the pesticidal bacterium Bacillus thuringiensis has been the subject of intensive research. These efforts have yielded considerable data about the complex relationships between the structure, mechanism of action, and genetics of the organism’s pesticidal crystal proteins, and a coherent picture of these relationships is beginning to emerge. Other studies have focused on the ecological role of the B. thuringiensis crystal proteins, their performance in agricultural and other natural settings, and the evolution of resistance mechanisms in target pests. Armed with this knowledge base and with the tools of modern biotechnology, researchers are now reporting promising results in engineering more-useful toxins and formulations, in creating transgenic plants that express pesticidal activity, and in constructing integrated management strategies to insure that these products are utilized with maximum efficiency and benefit.

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