Growth and yield of doubled haploid lines of oilseed Brassica rapa

1998 ◽  
Vol 78 (4) ◽  
pp. 537-544 ◽  
Author(s):  
D. B. Dewan ◽  
G. Rakow ◽  
R. K. Downey
Keyword(s):  
Brassica Rapa ◽  
Seed Yield ◽  
Doubled Haploid ◽  
Field Tests ◽  
Field Evaluation ◽  
Growth And Yield ◽  
Consistent Performance ◽  
Delayed Flowering ◽  
Dh Lines ◽  
Number Of Seeds

The production of doubled haploid (DH) lines of Brassica rapa could be an efficient procedure for the development of inbred parents for hybrid production. A total of 162 B. rapa DH lines were evaluated in field tests at Saskatoon, Canada, in single row, replicated tests and 10 DH lines were tested in four-row plot, multilocation, replicated tests. Seed of DH lines was produced by bud selfing in the greenhouse. Approximately one-fifth of all DH lines tested were chlorophyll deficient, presumably due to the expression of recessive alleles. Inbreeding depression was evident in low seed and biological yields, low number of seeds per pod and delayed flowering. Seed yield of DH lines was positively associated with the number of seeds per pod, early flowering and a long pod-filling period. One DH line was equal in yield to its donor population (DP), suggesting that dominance deviation was the genetic basis for high seed yield in this species. The consistent performance of DH lines over years and locations indicated that DH lines may be selected after 1 year of evaluation for combining ability testing. Higher yielding DH lines of B. rapa must be selected before they can be used as parents for hybrid development. Key words: Brassica rapa, doubled haploid, field evaluation

scholarly journals Using Anther Culture Method for Flax Breeding Intensification

2015 ◽  
Vol 1 ◽  
pp. 149
Author(s):  
Andra Miķelsone ◽  
Dace Grauda ◽  
Veneranda Stramkale ◽  
Reinis Ornicāns ◽  
Isaak Rashal
Keyword(s):  
Anther Culture ◽  
Culture Method ◽  
Field Evaluation ◽  
Vegetation Period ◽  
Fibre Content ◽  
Bast Fibre ◽  
Culture Methods ◽  
Dh Lines ◽  
Anther Cultures ◽  
Number Of Seeds

Flax breeding is a long and complicated process based on hybridization and following selection of the best plants. Because of possible occasional cross-pollination the development of genetically stable homozygous lines could last more than 15 years. For more rapid creating of initial material for flax breeding anther culture methods for producing doubled haploid (DH) lines could be used successfully. The goal of this study was to develop the best anther culture protocol for producing DH lines from hybrids included in Latvian flax breeding programme and to do preliminary field evaluation of obtained DH lines. F4 hybrids were used in the experiment. Method, most applicable for establishing of DH from anther cultures, was elaborated; 13 DH lines were obtained during the experiment. Such agronomic important traits, as vegetation period, total plant height, number of seed vessels, number of seeds in a seed vessel, 1000 seeds weight, oil and bast fibre content were evaluated for obtained DH lines. Several accessions showed high 1000 seeds weight, number of seeds in a seed vessel, good oil and bast fibre content. It was concluded that anther culture method is of value of using as an adjunct to classical methods of flax breeding.

scholarly journals Using Self-compatible Inbreds of Broccoli as Seed Producers

HortScience ◽  
2003 ◽  
Vol 38 (1) ◽  
pp. 85-87 ◽  
Author(s):  
Mark W. Farnham ◽  
Howard F. Harrison
Keyword(s):  
Seed Production ◽  
Doubled Haploid ◽  
Environmental Conditions ◽  
Seed Weight ◽  
Field Tests ◽  
Plant Production ◽  
Selfed Seed ◽  
Dh Lines ◽  
High Yields ◽  
Relative Productivity

The discovery that broccoli (Brassicaoleracea L., Italica Group) sprouts contain high levels of sulforaphane, a constituent that may provide chemoprotection against certain carcinogens, has stimulated much interest in seed production of this crop. Studies were undertaken to determine the potential for producing broccoli seed using self-compatible selections from open-pollinated (OP) populations or doubled-haploid (DH) programs. In all outdoor and greenhouse trials, three OP selections and seven DH lines produced selfed seed, but seed weight per plant and number per plant varied significantly among the entries. In all environments there were individuals with relatively high (i.e., >3 g/plant) production that were significantly different from low (i.e., <2 g/plant) producers. The relative productivity of some lines varied greatly between experiments, which indicates that seed production of particular genotypes is affected differently by environmental conditions. This indicates the importance of identifying lines that are high producers of selfed seed across different environments. Two OP cultivar-derived lines (USVL102 and USVL104) and two DH lines (USVL062 and USVL093) were identified that consistently produced relatively high yields in greenhouse and screen cage trials. These lines are good candidates for evaluating seed production in field tests and as possible sources of seed for sprouting.

scholarly journals Influence of Genotype on High Glucosinolate Synthesis Lines of Brassica rapa

2021 ◽  
Vol 22 (14) ◽  
pp. 7301
Author(s):  
Prabhakaran Soundararajan ◽  
Sin-Gi Park ◽  
So Youn Won ◽  
Mi-Sun Moon ◽  
Hyun Woo Park ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Brassica Rapa ◽  
Doubled Haploid ◽  
Industrial Production ◽  
Dry Weight ◽  
Nucleotide Polymorphisms ◽  
Phenethyl Isothiocyanate ◽  
Single Nucleotide ◽  
Pak Choi ◽  
Dh Lines

This study was conducted to investigate doubled haploid (DH) lines produced between high GSL (HGSL) Brassica rapa ssp. trilocularis (yellow sarson) and low GSL (LGSL) B. rapa ssp. chinensis (pak choi) parents. In total, 161 DH lines were generated. GSL content of HGSL DH lines ranged from 44.12 to 57.04 μmol·g−1·dry weight (dw), which is within the level of high GSL B. rapa ssp. trilocularis (47.46 to 59.56 μmol g−1 dw). We resequenced five of the HGSL DH lines and three of the LGSL DH lines. Recombination blocks were formed between the parental and DH lines with 108,328 single-nucleotide polymorphisms in all chromosomes. In the measured GSL, gluconapin occurred as the major substrate in HGSL DH lines. Among the HGSL DH lines, BrYSP_DH005 had glucoraphanin levels approximately 12-fold higher than those of the HGSL mother plant. The hydrolysis capacity of GSL was analyzed in HGSL DH lines with a Korean pak choi cultivar as a control. Bioactive compounds, such as 3-butenyl isothiocyanate, 4-pentenyl isothiocyanate, 2-phenethyl isothiocyanate, and sulforaphane, were present in the HGSL DH lines at 3-fold to 6.3-fold higher levels compared to the commercial cultivar. The selected HGSL DH lines, resequencing data, and SNP identification were utilized for genome-assisted selection to develop elite GSL-enriched cultivars and the industrial production of potential anti-cancerous metabolites such as gluconapin and glucoraphanin.

scholarly journals Performance of Soybean Under Different Levels of Phosphorus and Potassium

2016 ◽  
Vol 19 (1) ◽  
pp. 99-108 ◽  
Author(s):  
M Khanam ◽  
MS Islam ◽  
MH Ali ◽  
Imtiaz Faruk Chowdhury ◽  
SM Masum
Keyword(s):  
Seed Yield ◽  
Seed Weight ◽  
Maximum Yield ◽  
Growth And Yield ◽  
Combined Application ◽  
Stover Yield ◽  
Biological Yield ◽  
Phosphorus And Potassium ◽  
Pod Number ◽  
Number Of Seeds

A field experiment was conducted at the Sher-e-Bangla Agricultural University Farm, Dhaka, Bangladesh during December 2013 to April 2014 to evaluate the effect of phosphorus (P0: 0 kg TSP ha-1, P1: 100 kg TSP ha-1, P2: 175 kg TSP ha-1, P3: 250 kg TSP ha-1) and potassium (K0: 0 kg MoP, K1: 60 kg MoP ha-1, K2: 120 kg MoP ha-1, K3: 180 kg MoP ha-1), and their combinations on growth and yield of soybean (Glycine max). Number of nodules plant-1, number of filled pods plant-1, number of seeds pod-1, 1000-seed weight, seed yield, biological yield and harvest index increased significantly up to 175 kg ha-1 TSP. On the other hand, numbers of nodules plant-1, number of filled pods plant-1, length of pod, number of seeds pod-1, 1000-seed weight, seed yield, stover yield and biological yield were enhanced significantly up to 120 kg ha-1 MoP. The treatment of combined phosphorus @ 175 kg ha-1 and potassium @ 120 kg MoP ha-1 depicted the highest number of filled pods plant-1 (63.00), length of pod (3.16 cm), number of seeds pod-1 (3.11) vis a vis the highest (3.67 t ha-1) seed yield. Thus, the combined application of 175 kg ha-1 TSP and 120 kg ha-1 MoP could be the optimum for getting maximum yield of soybean.Bangladesh Agron. J. 2016, 19(1): 99-108

scholarly journals Effect of Phosphorus and Boron on the Growth and Yield of French Bean

2020 ◽  
pp. 18-25
Author(s):  
F. M. Jamil Uddin ◽  
Mohammad Hadiuzzaman ◽  
Harun Or Rashid ◽  
Saedy Karim
Keyword(s):  
Seed Yield ◽  
Seed Weight ◽  
Harvest Index ◽  
French Bean ◽  
Growth And Yield ◽  
Stover Yield ◽  
Biological Yield ◽  
Four Levels ◽  
Pod Length ◽  
Number Of Seeds

An experiment was conducted at the Agronomy Field Laboratory of the Department of Agronomy, Bangladesh Agricultural University, and Mymensingh to study the effect of phosphorus and boron on the growth and yield of var. BARI Jharsheem-3 along with French bean during November 2017 to March 2018. The experiment was comprised of two factors involving number of levels viz. phosphorus had four levels  15, 20, 25 and 35 kg ha-1 and four levels of boron viz. 0, 0.5, 1.0 and  1.5 kg  ha-1. Triple super phosphate and Borax were used as source of phosphorus and boron, respectively. The experiment was laid out in a Randomized Complete Block Design (RCBD) with three replications. The results exhibited that yield and its contributing characters were significantly influenced by the phosphorus and boron applications. The experimental findings revealed that highest  number of pods per  plant (4.95), pod length (13.06 cm), number of seeds per pod (4.34), and 1000-seed weight (427.99 g), seed yield (1.40 t ha-1), stover yield (1.76 t ha-1), biological yield (3.16 t ha-1) and harvest index (46%) were recorded under phosphorus level at 25 kg ha-1. The experimental findings also revealed that the highest number of pods per plant (4.91), pod length (14.45 cm), number of seeds  per pod (4.36), and 1000-seed weight (427.22 g), seed yield (1.39 t ha-1), stover yield (1.68 t ha-1), biological yield (3.07 t ha-1) and harvest index (45.27 %) were recorded  under boron level at 1.5 kg ha-1. In case of  interaction of phosphorus and boron, the highest number of pods  per plant (5.18),  pod length (14.35 cm), number of seeds pod-1 (4.84), and 1000-seed weight (427.19 g), seed yield (1.86 t ha-1), stover yield (2.05 t ha-1), biological yield (3.91 t ha-1) and harvest index (47.57 %) were recorded under P level at 25 kg  ha-1  and B level at1.5 kg ha-1. So it may be concluded that 25 kg P ha-1 and 1.5 kg B ha-1 could be applied to obtain higher yield of bean.

scholarly journals Effect of Nitrogen and Phosphorus Fertilization on the Growth and Yield of Mustard Under Irrigated Conditions in Agra District

2022 ◽  
Vol 22 (1) ◽  
pp. 151-154
Author(s):  
Avinash Singh ◽  
Keyword(s):  
Seed Yield ◽  
Agricultural Research ◽  
Growth And Yield ◽  
Phosphorus Fertilization ◽  
Rabi Season ◽  
Experimental Site ◽  
Nitrogen And Phosphorus ◽  
Additional Increase ◽  
N Level ◽  
Number Of Seeds

The present field experiment was conducted at Agricultural Research Farm of RBS College, Bichpuri Agra, during Rabi season of 2018-19. The soil of the experimental site is Gangetic alluvial. The experimental was laid out in RBD factorial having 3 main treatment (N level) and 4 sub treatments (P2O5 level) with 3 replications. All growth and yield attributing character increase with application of N @ 120 Kg-1 and P2O5 @ 60 kg-1. All the yield components i.e., number of siliquae plant-1, length of siliqua and number of seeds siliqua-1 improved with the increase in the level of nitrogen. Higher value of harvest index was associated with the application of 60 kg P2O5 ha-1. Seed yield increased appreciably with every additional increase in the level of nitrogen. Respectively 26.85 and 40.05 per cent higher seed yield ha-1 was obtained with the application of 80 and 120 kg ha-1 nitrogen as compared to 40 kg ha-1 .

scholarly journals Genetic variability and character association of Bangladeshi popular varieties of mustard (Brassica rapa L.)

2021 ◽  
Vol 44 (2) ◽  
pp. 95-107
Author(s):  
Fahmida Sultana ◽  
Jamilur Rahman ◽  
Md Mahmudul Hasan
Keyword(s):  
Genetic Variability ◽  
Brassica Rapa ◽  
Seed Yield ◽  
Seed Weight ◽  
Positive Association ◽  
Yield Attributes ◽  
High Heritability ◽  
Academy Of Sciences ◽  
Number Of Seeds ◽  
Additive Genes

A study was conducted to estimate genetic variability, correlation and direct and indirect effects of yield attributes on seed yield of fourteen Bangladeshi varieties of Brassica rapa. High heritability coupled with high genetic advance was recorded in a number of secondary branches plant-1, a number of siliqua plant-1 and a number of seeds siliqua-1 indicating the effects of additive genes in controlling the traits. Significant and positive association with seed yield plant-1 were observed in the number of primary branches plant-1, a number of seeds siliqua-1, while a negative association was found in days to maturity. The path analysis revealed that the days to first flowering, number of secondary branches plant-1 and 1000 seed weight were the main contributors to seed yield. The results suggest that days to first flowering, number of primary and secondary branches plant-1 and 1000 seed weight may be considered for selection to improve the seed yield in B. rapa. Journal of Bangladesh Academy of Sciences, Vol. 44, No. 2, 95-107, 2020

Effect of phosphorus and potassium on the growth and yield of French bean

2020 ◽  
pp. 108-112
Author(s):  
Saedy Karim ◽  
F. M. Jamil Uddin ◽  
Harun Or Rashid ◽  
Mohammad Hadiuzzaman
Keyword(s):  
Seed Yield ◽  
Harvest Index ◽  
French Bean ◽  
Growth And Yield ◽  
Yield Response ◽  
Stover Yield ◽  
Biological Yield ◽  
Phosphorus And Potassium ◽  
Pod Length ◽  
Number Of Seeds

An experiment was conducted at the Agronomy Field Laboratory of the Department of Agronomy, Bangladesh Agricultural University, Mymensingh to investigate the yield response of French bean (Phaseolus vulgaris L.) as affected by phosphorus and potassium management. The experiment consisted of two factors. Factor A: Phosphorus fertilizer (4 levels) viz. P1=15Kg P ha-1, P2=20 Kg P ha-1 and P3=25 Kg P ha-1 , P4=35 Kg P ha-1 and factor B: Potassium fertilizer (4 levels) viz. K1=0 Kg K ha-1, K2=20 Kg K ha-1, K3=30 Kg K ha-1 and K4=40 Kg K ha-1. The variety BARI Jharsheem-3 was used in this experiment as the test crop. The experiment was laid out in a randomized complete block design (RCBD) with three replications. In case of phosphorus @ 25 kg ha-1  the highest number of pods plant-1 (4.96), pod length (13.34 cm), number of seeds pod-1 (4.54), weight of 1000-seed (431.21 g), seed yield (1.33 t ha-1), stover yield (1.53 t ha-1), biological yield (2.86 t ha-1) and harvest index 46.50%  were found. In case of potassium @ 40 kg ha-1, he highest number of pods plant-1 (5.80), pod length (14.89 cm), number of seeds pod-1 (4.24), weight of 1000-seed (430.37 g), seed yield (1.53 t ha-1 ), stover yield (1.46 t ha-1 ), biological yield (2.99 t ha-1) and harvest index 51.17% were found. Interaction effect of phosphorus and potassium the highest  number of pods plant-1 (5.18), pod length (13.40 cm), number of seeds pod-1 (4.30), weight of 1000-seed (430.53 g), seed yield (1.40 t ha-1 ), stover yield (1.60 t ha-1 ), biological yield (3.00 t ha-1 ) and harvest index 46.66% were recorded from P @ 25 kg ha-1 and K @ 40 kg ha-1. However, from the study it can be concluded that application of P @ 25 kg ha-1 and K @ 40 kg ha-1 was the most suitable combination for better yield of French bean.

scholarly journals Growth, Yield and Yield Components of Lentil as Influenced by Irrigation and Weeding Regime

2015 ◽  
Vol 7 (2) ◽  
pp. 155-161
Author(s):  
MB Hossain ◽  
MZF Begum ◽  
MS Alam ◽  
MM Hasan ◽  
MR Amin
Keyword(s):  
Seed Yield ◽  
Growth Yield ◽  
Growth And Yield ◽  
Stover Yield ◽  
Four Levels ◽  
Growth Character ◽  
Number Of Seeds ◽  
Filled Seeds ◽  
Number Of Branches ◽  
Plot Design

The experiment was carried out to study the effect of irrigation and weeding regime on growth, yield and yield contributing characters of lentil. The experiment was laid out in a split-split plot design with three replications. Four levels of irrigation viz. control or no irrigation (I0), one irrigation (I1), two irrigation (I2) and three irrigations (I3) and three weeding regime viz. control weeding (W0), one weeding (W1) and two weedings (W2) were used. Irrigation had significant effect on all the growth, yield and yield contributing characters. The highest TDM , CGR and all the yield contributing characters like plant height, number of branches plant-1, number of pods plant-1, number of effective pods plant-1, number of seeds plant-1, number of filled seeds plant-1, 1000-seed weight and stover yield were obtained from three irrigations (I3) and consequently it produced the highest seed yield. Effect of weeding regime was significant in respect of on the growth, yield and yield contributing characters. All the yield contributing characters except non-effective pods plant-1 were found the best when two weedings were done and as a result it produced the highest seed yield. With some exception two weedings showed highest values of TDM, CGR and other growth character. Finally the results suggest that three irrigations with two weedings are better practices to get higher growth and yield of lentil.DOI: http://dx.doi.org/10.3329/jesnr.v7i2.22225 J. Environ. Sci. & Natural Resources, 7(2): 155-161 2014

scholarly journals Evaluation of Doubled Haploid Derivatives of the Flue-cured Nicotianatabacum cv. Wislica

2006 ◽  
Vol 22 (2) ◽  
pp. 107-113
Author(s):  
A Berbec ◽  
D Laskowska
Keyword(s):  
Doubled Haploid ◽  
Unit Area ◽  
Chromosome Doubling ◽  
Growth Parameters ◽  
Parental Genotype ◽  
True Breeding ◽  
Delayed Flowering ◽  
Dh Lines ◽  
Derivatives Of ◽  
Parental Cultivar

AbstractA total of 51 doubled haploid (DH) derivatives of the flue-cured true-breeding tobacco variety Wislica were obtained via anther culture and colchicine-induced chromosome doubling. Of that number eight DH lines showed morphological aberrations and/or reduced vigour that made them unfit to be grown in the field. The remaining 43 DH lines were grown in 2001 under regular flue-cured tobacco management and evaluated for principal growth indicators, yield, curability and gross money returns from unit area. In 2002, the evaluation was scaled down to include 13 best performing DH lines of 2001. The DH lines showed a considerable variation for basic growth parameters. Usually, the DH lines had more leaves but were shorter than the parental genotype mostly due to shorter internodes. The majority of DH lines showed delayed flowering compared to the parental cultivar. Yields of cured leaves varied extensively, some of the DH lines yielding above and some below cv. Wislica. Curability measured as percentage of light (1st to 3rd) grades was generally lower in DH lines compared to that in cv. Wislica. However, because of the high leaf yield some of the DH lines were not inferior to the parental cultivar with respect to money returns from unit area.

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