Detection ofEscherichia coliO157 andEscherichia coliO157:H7 by the immunomagnetic separation technique andstx1andstx2genes by multiplex PCR in slaughtered cattle in Samsun Province, Turkey

Journal of Veterinary Science ◽

10.4142/jvs.2010.11.4.321 ◽

2010 ◽

Vol 11 (4) ◽

pp. 321 ◽

Author(s):

Gökhan Inat ◽

Belgin Siriken

Keyword(s):

Multiplex Pcr ◽

Immunomagnetic Separation ◽

Separation Technique

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Detection of Escherichia coli O157:H7 in ground beef using immunomagnetic separation and multiplex PCR

Food Control ◽

10.1016/j.foodcont.2008.06.002 ◽

2009 ◽

Vol 20 (4) ◽

pp. 357-361 ◽

Author(s):

Belgin Sarimehmetoglu ◽

Mihriban Hatun Aksoy ◽

Naim Deniz Ayaz ◽

Yildiz Ayaz ◽

Ozlem Kuplulu ◽

...

Keyword(s):

Escherichia Coli ◽

Multiplex Pcr ◽

Ground Beef ◽

Immunomagnetic Separation ◽

Escherichia Coli O157

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Development of immunomagnetic separation technique for isolation ofPseudomonas syringae pv. phaseolicola

Folia Microbiologica ◽

10.1007/bf02817554 ◽

2000 ◽

Vol 45 (4) ◽

pp. 321-324 ◽

Author(s):

K. Güven ◽

M. B. Mutlu

Keyword(s):

Immunomagnetic Separation ◽

Separation Technique

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The prevalence of Escherichia coli O157 and O157:H7 in ground beef and raw meatball by immunomagnetic separation and the detection of virulence genes using multiplex PCR

Meat Science ◽

10.1016/j.meatsci.2009.10.011 ◽

2010 ◽

Vol 84 (3) ◽

pp. 553-556 ◽

Author(s):

Özgür Çadırcı ◽

Belgin Sırıken ◽

Gökhan Inat ◽

Tahsin Onur Kevenk

Keyword(s):

Escherichia Coli ◽

Multiplex Pcr ◽

Virulence Genes ◽

Ground Beef ◽

Immunomagnetic Separation ◽

Escherichia Coli O157

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One-Step Purification of a Fusion Protein of Glucagon-Like Peptide-1 and Human Serum Albumin Expressed inPichia pastorisby an Immunomagnetic Separation Technique

Bioscience Biotechnology and Biochemistry ◽

10.1271/bbb.70190 ◽

2007 ◽

Vol 71 (11) ◽

pp. 2655-2662 ◽

Author(s):

Jiaqi CHEN ◽

Gang BAI ◽

Yu CAO ◽

Zhihui GAO ◽

Qi ZHANG ◽

...

Keyword(s):

Human Serum Albumin ◽

Fusion Protein ◽

Serum Albumin ◽

Human Serum ◽

Immunomagnetic Separation ◽

Separation Technique ◽

One Step ◽

Glucagon Like Peptide ◽

Glucagon Like Peptide 1

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Detection of genetic diversity by pulsed-field gel electrophoresis among Escherichia coli O157 isolated from bovine faecal samples by immunomagnetic separation technique

Letters in Applied Microbiology ◽

10.1111/j.1472-765x.2006.02034.x ◽

2007 ◽

Vol 44 (1) ◽

pp. 19-23 ◽

Author(s):

L. Vali ◽

A. Hamouda ◽

M.C. Pearce ◽

H.I. Knight ◽

J. Evans ◽

...

Keyword(s):

Escherichia Coli ◽

Genetic Diversity ◽

Gel Electrophoresis ◽

Immunomagnetic Separation ◽

Pulsed Field Gel Electrophoresis ◽

Separation Technique ◽

Escherichia Coli O157 ◽

Pulsed Field ◽

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Use of Immunomagnetic Separation Technique and Polymerase Chain Reaction for Detection of Verocytotoxin-Producing Escherichia coli in Cattle at Slaughter

Veterinary Research Communications ◽

10.1007/s11259-005-0072-7 ◽

2005 ◽

Vol 29 (S2) ◽

pp. 323-326

Author(s):

S. Bonardi ◽

C. Bacci ◽

G. Pizzin ◽

B. Orlandini ◽

E. Donati ◽

...

Keyword(s):

Escherichia Coli ◽

Polymerase Chain Reaction ◽

Immunomagnetic Separation ◽

Separation Technique ◽

Chain Reaction ◽

Polymerase Chain

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Detection and Identification of Salmonella Typhimurium in Bovine Diarrhoeic Fecal Samples by Immunomagnetic Separation and Multiplex PCR Assay

Zoonoses and Public Health ◽

10.1111/j.1863-2378.2007.01061.x ◽

2007 ◽

Vol 54 (6-7) ◽

pp. 231-236 ◽

Author(s):

T. Zahraei Salehi ◽

H. Tadjbakhsh ◽

N. Atashparvar ◽

M. G. Nadalian ◽

M. R. Mahzounieh

Keyword(s):

Salmonella Typhimurium ◽

Multiplex Pcr ◽

Immunomagnetic Separation ◽

Pcr Assay ◽

Fecal Samples ◽

Multiplex Pcr Assay ◽

Detection And Identification

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Large-volume immunomagnetic separation combined with multiplex PCR assay for simultaneous detection of Listeria monocytogenes and Listeria ivanovii in lettuce

Food Control ◽

10.1016/j.foodcont.2015.06.048 ◽

2016 ◽

Vol 59 ◽

pp. 601-608 ◽

Author(s):

Yan Mao ◽

Xiaolin Huang ◽

Sicheng Xiong ◽

Hengyi Xu ◽

Zoraida P. Aguilar ◽

...

Keyword(s):

Listeria Monocytogenes ◽

Multiplex Pcr ◽

Large Volume ◽

Simultaneous Detection ◽

Immunomagnetic Separation ◽

Pcr Assay ◽

Listeria Ivanovii ◽

Multiplex Pcr Assay

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Evaluation of a modified semi-automated immunomagnetic separation technique for the detection of Cryptosporidium oocysts in human faeces

Journal of Microbiological Methods ◽

10.1016/j.mimet.2008.04.006 ◽

2008 ◽

Vol 75 (1) ◽

pp. 139-141 ◽

Author(s):

G. Robinson ◽

J. Watkins ◽

R.M. Chalmers

Keyword(s):

Immunomagnetic Separation ◽

Separation Technique ◽

Human Faeces ◽

Cryptosporidium Oocysts

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Comparison between immunomagnetic separation, coupled with immunofluorescence, and the techniques of Faust et al. and of Lutz for the diagnosis of Giardia lamblia cysts in human feces

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652003000600008 ◽

2003 ◽

Vol 45 (6) ◽

pp. 339-342 ◽

Author(s):

Doris Sobral Marques Souza ◽

Juliana Teixeira Barreiros ◽

Karina Maria Papp ◽

Mário Steindel ◽

Cláudia Maria Oliveira Simões ◽

...

Keyword(s):

Data Analysis ◽

Giardia Lamblia ◽

Recovery Rate ◽

Immunomagnetic Separation ◽

Separation Technique ◽

Sample Storage ◽

Human Feces ◽

Routine Procedure ◽

Higher Sensitivity

In the present study, the performance of Immunomagnetic Separation technique, coupled with Immunofluorescence (IMS-IFA), was compared with the FAUST et al. and Lutz parasitological techniques for the detection of Giardia lamblia cysts in human feces. One hundred and twenty-seven samples were evaluated by the three techniques at the same time showing a rate of cyst detection of 27.5% by IMS-IFA and 15.7% by both Faust et al. and Lutz techniques. Data analysis showed a higher sensitivity of IMS-IFA for the detection of G. lamblia cysts in comparison with the techniques of FAUST et al. and Lutz. The use of this methodology as a routine procedure enables the processing of many samples simultaneously, in order to increase recovery rate of G. lamblia cysts and reduce the time of sample storage.

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